FastQCFastQC Report
Fri 17 Jun 2016
SRR1527550_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527550_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences402530
Sequences flagged as poor quality0
Sequence length52
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG29260.726902342682533No Hit
ATTTGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC12680.31500757707500066No Hit
ATTTGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC9820.24395697215114398No Hit
ATTTGCGGGGGCGGAGTCCGGCTCTCGCAACCCAAGATGGCGGATGAGAGTG9280.23054182296971654No Hit
ATTTGCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA8200.2037115246068616No Hit
ATTTGCGGGGGTCCTCCCGCGCCTCCTCCACGACCTCCAGTGAGCCTCCGCG7450.1850793729659901No Hit
ATTTGCGGGATCTCTAACCATGCCTACTAACCAGAGCTGAACTAAGGCTGCT7330.18209822870345066No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGCGCTTAAGAGCGCAGACTGCTC7170.17812336968673143No Hit
ATTTGCGGGATCCTCACCCTGCCACCCTAACCCACTAAGTGCCTGTGCGCAT7120.17688122624400668No Hit
ATTTGCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC7060.17539065411273694No Hit
ATTTGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT6740.16744093607929844No Hit
ATTTGCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT6720.1669440787022085No Hit
ATTTGCGGGAGGAACCAACCTATGTAAAAGTAAAATAAGAAAGGAAGGAATC6690.16619879263657367No Hit
ATTTGCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTC6630.16470822050530395No Hit
ATTTGCGGGGGACTCTGAGGCTCTTCCGCTGAGTTTAACTAGCTGTGGCTGT6600.16396293443966908No Hit
ATTTGCGGGAGCGTCTTCCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCG6510.16172707624276453No Hit
ATTTGCGGGATGGTGACACCCATTTGTAATCCCAGCAAGTGGGAGGTAGAGT6230.1547710729635058No Hit
ATTTGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT5930.14731821230715722No Hit
ATTTGCGGGATCTGACACTGCCTTCCTTCTTGGCCCTGTGGTACTACTGTCT5820.14458549673316273No Hit
ATTTGCGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAA5750.14284649591334808No Hit
ATTTGCGGGTCTCGCAACCCAAGATGGCGGATGAGAGTGAGACAGCAGTGAA5680.1411074950935334No Hit
ATTTGCGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAAT5560.13812635083099398No Hit
ATTTGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG5500.13663577869972424No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGTTAAGAGCTCTGACTACTCT5470.1358904926340894No Hit
ATTTGCGGGAGTGAAAATATTTTTAAGGTCTAATCAAGTGATTTTTTCCCCC5400.13415149181427471No Hit
ATTTGCGGGGATCTCAGCCATGGGCAAAAAGCACAAGAAGCACAAGGCGGAA5290.13141877624028023No Hit
ATTTGCGGGATGTGAACCCACTTCCGACCGGCTATGAAGATGAGTAAAGAGA5250.13042506148610042No Hit
ATTTGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT5240.13017663279755548No Hit
ATTTGCGGGAGATTATTGAAGTTTCCACAGACGGTCAGCTGTTGAGAGGTTC5190.1289344893548307No Hit
ATTTGCGGGGGTATCAATGAGAGTGCAGTGCTATTTGCGGGTGCAAAGCTGC5190.1289344893548307No Hit
ATTTGCGGGGAGTAGCCGGACCCCGCCTGCGCCCCAGGGGGTGCCTCCTCTC5180.12868606066628574No Hit
ATTTGCGGGAGACGCGGGCCCGCCATGGAGCACATCCGCACGCCCAAGGTTG5110.12694705984647106No Hit
ATTTGCGGGGCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTCGCGTGA5040.12520805902665638No Hit
ATTTGCGGACTCGCCCTGTATTGACCAAGGAGCAGCTGGACAACCAATTGGA5000.1242143442724766No Hit
ATTTGCGGGTGCTGTGCTGCCCATGGTGGCGCATGCCTTTAATCCCAGCACT5000.1242143442724766No Hit
ATTTGCGGGACCAAAGTGATCCTTCACCTCAAAGAAGACCAGACAGAGTACT4990.12396591558393162No Hit
ATTTGCGGGGAGCCACGCCCGCAACGCGAGCTGAGCAACGCCGAAGACAATG4900.12173005738702705No Hit
ATTTGCGGGGGAGACTCGCCATATTGTGCGGCGGCGCCGGCGGTTGATTCTC4860.12073634263284724No Hit
ATTTGCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT4830.11999105656721237No Hit
ATTTGCGGGGGCCCCAGCCGCCGCCAGCACCATGGCCAGCACCGTATCTCCC4690.11651305492758304No Hit
ATTTGCGGGGAAACGCTGGCTAAAGCGGTGTTGGCGGAGCTGCCTCAGCAAG4690.11651305492758304No Hit
ATTTGCGGGGATTGTTACCCAACAGTAAGGGCCTTTTCTTCCCTGTCAGTCA4680.11626462623903809No Hit
ATTTGCGGGGCTCTTTCGAGTCTTGGCTCCAAGATGACCAAAAAAAGAAGAA4550.1130350532879537No Hit
ATTTGCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT4520.11228976722231883No Hit
ATTTGCGGGGGCCGCCCTGAGTCACTGCCTGCGCAGCTCCCGCCGCCCGGCT4480.11129605246813902No Hit
ATTTGCGGCACTTCAAAGCAGCGAGGATTGCTATTGAGATCTATTTGAAGCT4460.11079919509104912No Hit
ATTTGCGGGAAGGCTGAGCTGGAGAAGCTGGACCTCAATGGCAATGCCCTCG4380.1088117655826895No Hit
ATTTGCGGAGAGTGCAGTGCTATTTGGGGGGATGCAAGAGCCTAGTGCGGTG4350.10806647951705463No Hit
ATTTGCGGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTC4270.106079050008695No Hit
ATTTGCGGGGGAGTACCAGCTCCCTATTACCCTGAGGGCGGGCGAGTGCAGC4270.106079050008695No Hit
ATTTGCGGGGCCAGACCCGCCCCGACTGGGGCTCGCCGTCGCCAGCAGTCAG4210.1045884778774253No Hit
ATTTGCGGGCATCTTGTGGGAAGAGCTGAAGCAGGCGCTTTTGGCTCGGCGC4180.10384319181179043No Hit
ATTTGCGGGATTATATATTCACTTCAAGAAGTCACTGTCAGAGAAGTGAAGA4160.10334633443470052No Hit
ATTTGCGGGTACTCAAAGAAAACAAAATTTTTATGTGCTGTGCGAATGGTTC4160.10334633443470052No Hit
ATTTGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC4130.10260104836906567No Hit
ATTTGCGGGGGCTTGGGACGCCGCGGATCTGCGGCGGCGACAGTTGGAGAGG4110.10210419099197575No Hit
ATTTGCGGGAGCTATGTCCGCAAGCCGACCAGGGAAACCCTAGAGCTTTGAG4070.10111047623779594No Hit
ATTTGCGGGACGCCATCCTTGCCTCAGCTTCTGATGTCCCCCTTCCTCCCAC4060.10086204754925099No Hit
ATTTGCGGGGACCCTCGGCTCCGGAGCGGCAGTGCCGTCCCTCTCGGCGCCT4060.10086204754925099No Hit
ATTTGCGGGGGCACTGGGCGGGATCTGGGGGGGTCTCCGTGCTGATTCCCAT4040.10036519017216107No Hit
ATTTGCAGGGGCCTGGTGCTGGAACCAGGAAGGCGCTGAGCTTAAATTGAAG4040.10036519017216107No Hit
ATTTGCGGGATTGAACGCTTAGCTTCAGCCTCATTCCTAGCAATCCATGCAA4030.10011676148361612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGAA351.0173608E-746.00000439
CTACGGG351.0173608E-746.00000428
CCTCACG351.0173608E-746.00000443
CGGGATA5800.046.0000046
TTGCGAG1450.046.0000043
AACGAAG351.0173608E-746.00000436
GAACGAA351.0173608E-746.00000435
CTACCGT351.0173608E-746.00000420
CGATCGT351.0173608E-746.00000424
AATACAC351.0173608E-746.00000418
CTAATAG351.0173608E-746.00000443
TGCAATA351.0173608E-746.00000428
GTTGCCG351.0173608E-746.00000422
ACGAAGG351.0173608E-746.00000441
ACAACAT206.306543E-446.029
AACCGCC206.306543E-446.021
ATCTCGG253.4131725E-546.014
ACCGGGG206.306543E-446.029
AATTTCG253.4131725E-546.039
CTCGTCA253.4131725E-546.011
Produced by FastQC (version 0.11.5)