FastQCFastQC Report
Fri 17 Jun 2016
SRR1527549_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527549_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4038817
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT182310.45139455439550735No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGA182030.45070128208334276No Hit
GGACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC176550.43713295254526263No Hit
GGACATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC138300.342427002758481No Hit
GGACATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA133930.33160700274362515No Hit
GGACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG129990.3218516709224508No Hit
GGACATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT128750.3187814649685787No Hit
GGACATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC122940.30439606449116163No Hit
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA113730.28159235736603067No Hit
GGACATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC103930.257327826440267No Hit
GGACATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC99750.2469782612086658No Hit
GGACATGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG95130.23553926805794864No Hit
GGACATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG74460.1843609155849349No Hit
GGACATGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA73830.18280105288256437No Hit
GGACATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA70040.17341711694290682No Hit
GGACATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG70020.1733675974920379No Hit
GGACATGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG63780.1579175288209394No Hit
GGACATGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA62890.1557139132572731No Hit
GGACATGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC60430.14962302080039774No Hit
GGACATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC59960.14845931370497847No Hit
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA59490.14729560660955918No Hit
GGACATGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG57530.14244270042440646No Hit
GGACATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA56380.13959533199944438No Hit
GGACATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT56310.13942201392140322No Hit
GGACATGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC53660.13286068668127324No Hit
GGACATGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGG52490.1299637988054423No Hit
GGACATGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC47880.1185495653801596No Hit
GGACATGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT47830.11842576675298731No Hit
GGACATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG47780.11830196812581506No Hit
GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG47430.11743537773560922No Hit
GGACATGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT41500.10275286055297875No Hit
GGACATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT41020.10156439373212503No Hit
GGACATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTC40910.10129203675234605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAT218550.042.409525
ATGGGGC479600.041.6513755
ATGGGAC159350.041.570135
ATGGGAG402750.041.4435735
TGGGATT77000.041.26
CATGGGG2460550.041.1352734
ATGGGCT99900.041.1151125
ATGGGGT379950.040.8848535
ATGGGGA704950.040.8230365
ATGGGGG906500.040.7357985
ACATGGG4185400.040.7321173
TGGGGCT225850.040.6685836
GGACATG4350300.040.6319541
GACATGG4309200.040.6212772
TGGGGTT87700.040.53826
TGGGATA30300.040.514856
TGGGGTC93850.040.460316
TGGGGAC126400.040.2231036
TGGGGGC205000.040.1902476
CATGGGA1193350.040.146984
Produced by FastQC (version 0.11.5)