SRR1527548_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527548_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3390487
Sequences flagged as poor quality	0
Sequence length	52
%GC	55

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGACATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	15169	0.44739885450084305	No Hit
GGACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	14028	0.41374587190571743	No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAG	12031	0.3548457787922502	No Hit
GGACATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	11468	0.3382404946546027	No Hit
GGACATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	11386	0.33582196303952794	No Hit
GGACATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	11334	0.33428826006411466	No Hit
GGACATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	11105	0.3275340681146986	No Hit
GGACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	10838	0.3196590932217112	No Hit
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9632	0.2840889819073189	No Hit
GGACATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	9523	0.2808741045165488	No Hit
GGACATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	9246	0.2727041867436743	No Hit
GGACATGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	8140	0.24008350422815364	No Hit
GGACATGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	6403	0.1888519259917528	No Hit
GGACATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	6377	0.18808507450404618	No Hit
GGACATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	6291	0.18554856573701656	No Hit
GGACATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	6183	0.18236318263423512	No Hit
GGACATGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	5922	0.1746651734691801	No Hit
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5497	0.16213010107397552	No Hit
GGACATGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	5453	0.16083235240247198	No Hit
GGACATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	5407	0.159475615154991	No Hit
GGACATGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	5395	0.1591216836991264	No Hit
GGACATGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	5205	0.15351776898127023	No Hit
GGACATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	4706	0.13880011927490063	No Hit
GGACATGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	4615	0.1361161390679274	No Hit
GGACATGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA	4576	0.13496586183636747	No Hit
GGACATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	4532	0.13366811316486393	No Hit
GGACATGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	3971	0.11712181760319387	No Hit
GGACATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	3941	0.11623698896353239	No Hit
GGACATGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	3822	0.11272716869287508	No Hit
GGACATGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	3759	0.11086902854958594	No Hit
GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	3744	0.11042661422975521	No Hit
GGACATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	3713	0.10951229130210499	No Hit
GGACATGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	3474	0.1024631564728017	Illumina Single End Adapter 2 (97% over 34bp)
GGACATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	3434	0.10128338495325304	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCGAT	35	1.0203621E-7	46.000004	41
ATCGTTA	25	3.418576E-5	46.0	15
ATGGGAT	18755	0.0	44.45481	5
ATGGGGC	40800	0.0	43.50833	5
ATGGGAG	33500	0.0	43.466568	5
TGGGATT	6410	0.0	43.27301	6
ATGGGAC	13005	0.0	43.188004	5
ATGGGCT	8675	0.0	42.95101	5
CATGGGG	209015	0.0	42.897976	4
ACATGGG	350580	0.0	42.709877	3
ATGGGGT	32095	0.0	42.653374	5
GGACATG	364045	0.0	42.63319	1
GACATGG	360925	0.0	42.60345	2
ATGGGGG	78085	0.0	42.580265	5
TGGGGCT	19380	0.0	42.47523	6
TATAGTC	65	0.0	42.461536	19
TGGGATC	6090	0.0	42.147785	6
CATGGGA	97750	0.0	42.143528	4
TGGGGAC	10920	0.0	41.99817	6
ATGGGGA	59465	0.0	41.996803	5

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content