##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527548_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3390487
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73611755479375	33.0	31.0	34.0	30.0	34.0
2	32.33131877514941	34.0	31.0	34.0	30.0	34.0
3	32.107496061775194	33.0	31.0	34.0	30.0	34.0
4	36.25373817979541	37.0	37.0	37.0	35.0	37.0
5	35.47861767350826	37.0	35.0	37.0	33.0	37.0
6	36.11391814804186	37.0	35.0	37.0	35.0	37.0
7	36.49182167635505	37.0	37.0	37.0	35.0	37.0
8	36.611553443502366	37.0	37.0	37.0	35.0	37.0
9	38.591313283312985	39.0	39.0	39.0	39.0	39.0
10	37.42874430723374	39.0	37.0	39.0	34.0	39.0
11	37.46129213885793	39.0	37.0	39.0	35.0	39.0
12	37.29983303283569	39.0	37.0	39.0	35.0	39.0
13	37.25236993977561	39.0	37.0	39.0	34.0	39.0
14	38.06793861766761	40.0	38.0	40.0	34.0	40.0
15	38.235784121868036	40.0	38.0	40.0	35.0	40.0
16	38.26890060336465	40.0	38.0	40.0	35.0	40.0
17	38.25667581087909	40.0	38.0	40.0	35.0	40.0
18	38.15614069601211	40.0	38.0	40.0	34.0	40.0
19	38.00957060150946	40.0	38.0	40.0	34.0	40.0
20	38.008636222465974	40.0	38.0	40.0	34.0	40.0
21	37.99938946823863	40.0	38.0	40.0	34.0	40.0
22	37.97247150630573	40.0	38.0	40.0	34.0	40.0
23	37.836859129676654	40.0	37.0	40.0	34.0	40.0
24	37.73099852617043	40.0	37.0	40.0	34.0	40.0
25	37.58785655276071	39.0	37.0	40.0	34.0	40.0
26	37.330867512543186	39.0	35.0	40.0	34.0	40.0
27	37.20407333813697	39.0	35.0	40.0	33.0	40.0
28	37.028895848885426	39.0	35.0	40.0	33.0	40.0
29	36.92592067157314	39.0	35.0	40.0	33.0	40.0
30	36.645976816899754	39.0	35.0	40.0	32.0	40.0
31	36.57854845041435	39.0	35.0	40.0	32.0	40.0
32	36.3280546422977	39.0	35.0	40.0	31.0	40.0
33	36.08481613408339	38.0	35.0	40.0	30.0	40.0
34	35.589706139560484	38.0	35.0	40.0	28.0	40.0
35	35.376649726130786	38.0	35.0	40.0	27.0	40.0
36	35.143958375301246	38.0	35.0	40.0	26.0	40.0
37	34.6751906142097	38.0	34.0	40.0	23.0	40.0
38	34.61859255027375	38.0	34.0	40.0	23.0	40.0
39	34.298927263251564	38.0	34.0	40.0	21.0	40.0
40	33.83151889389342	38.0	33.0	40.0	17.0	40.0
41	33.6082256029886	38.0	33.0	40.0	15.0	40.0
42	33.394412661071996	37.0	33.0	40.0	15.0	40.0
43	33.22025301969894	37.0	33.0	40.0	13.0	40.0
44	33.05218984765315	37.0	33.0	40.0	12.0	40.0
45	32.91107236217098	36.0	33.0	40.0	11.0	40.0
46	32.75211732119899	36.0	33.0	40.0	10.0	40.0
47	32.60392327119968	35.0	32.0	40.0	10.0	40.0
48	32.38640260234002	35.0	32.0	39.0	10.0	40.0
49	32.18598419637061	35.0	31.0	39.0	10.0	40.0
50	32.393628407954374	35.0	32.0	39.0	10.0	40.0
51	32.445169381271775	35.0	33.0	39.0	10.0	40.0
52	32.06751773417801	35.0	32.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	34.0
12	48.0
13	61.0
14	116.0
15	253.0
16	458.0
17	962.0
18	1710.0
19	3003.0
20	4993.0
21	8007.0
22	12782.0
23	19862.0
24	28860.0
25	39554.0
26	56288.0
27	75866.0
28	91814.0
29	90270.0
30	74598.0
31	71198.0
32	85101.0
33	114489.0
34	210530.0
35	222606.0
36	356492.0
37	578761.0
38	1009329.0
39	232436.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.4819033371902	0.29600467425476046	0.13933101645869753	0.08276097209633897
8	98.83813151326049	0.7582686499019167	0.2762140070143316	0.1273858298232673
9	96.73197390227422	1.6735353947677722	0.7764961198789436	0.817994583079068
10	58.85856515597907	27.80924982163329	5.667474908471851	7.664710113915789
11	34.937576814186286	30.123460140091968	17.558215088274927	17.38074795744682
12	30.1212775627808	24.80180575828782	23.00530867689509	22.071608002036285
13	22.97584388319436	25.927160316497304	24.730724524235015	26.366271276073324
14	23.884887333294596	28.40668611913274	25.407824893591986	22.30060165398068
15	26.46714174099473	25.858173176891697	23.542547132609563	24.13213794950401
16	27.198629577402894	26.96096460478981	23.12776896062424	22.712636857183057
17	29.999731601979303	26.951290478329515	19.46245480369044	23.58652311600074
18	28.71165705693607	26.522030610941734	20.112302450945837	24.65400988117636
19	27.54713408427757	27.562176171151815	21.332009236431226	23.55868050813939
20	26.83228102629504	30.29558880479412	20.055850383735436	22.816279785175404
21	28.24594224959423	27.092922049251335	22.871434103714307	21.789701597440132
22	28.040367062312875	27.42664991784366	20.368224387823933	24.16475863201953
23	27.65172082948556	29.56351108262618	19.491241228767432	23.293526859120828
24	27.106400938862173	29.463938366376276	19.4162962429881	24.013364451773448
25	26.31409588062128	26.960079776150152	19.861453531601803	26.864370811626763
26	27.74896349698436	27.044521922661847	19.472305895878677	25.734208684475124
27	26.464251300771835	25.813666296316722	19.630837693818027	28.091244709093417
28	28.550293807349796	29.68676771213103	17.76381976984427	23.999118710674896
29	29.855622510866432	28.05243022610026	18.740464128014647	23.351483135018654
30	27.818894453805605	28.793385728952803	19.244993418349633	24.14272639889196
31	30.879310258378812	27.813791941983556	18.50530616988061	22.801591629757024
32	31.171598652346994	26.862955085803307	20.12424763758127	21.841198624268433
33	32.06217867816629	27.232105594270084	19.024110695602136	21.681605031961485
34	27.781407213771946	29.860607045536526	20.900950217476133	21.457035523215396
35	27.99712843612142	31.975141034311587	20.29360383921248	19.73412669035451
36	30.04332415962663	30.5209546593159	17.351401140898048	22.084320040159422
37	29.330801150395207	30.095175117910788	19.01602926069323	21.557994471000775
38	31.879609035516136	28.593207996373383	18.524978859969085	21.0022041081414
39	31.069961335937872	26.077699162391717	18.384792509158714	24.467546992511696
40	29.831289723275745	26.189747962460853	19.930971568391207	24.047990745872198
41	29.56062064240329	25.43566750145333	19.52232820830754	25.48138364783584
42	30.041996916667134	23.52290393680908	20.577073441072034	25.858025705451755
43	28.16043830871494	23.825839768741186	22.344076234476052	25.66964568806782
44	28.263107925203666	22.94859116109279	22.00716298278094	26.78113793092261
45	28.42983913520388	24.520489239451443	21.557021159497147	25.492650465847532
46	28.192144668302817	24.320075552568113	22.61229728944544	24.875482489683634
47	29.102131935618686	24.85607524818706	21.320860395571493	24.72093242062276
48	29.4048023189589	23.94732674096671	22.32413809579568	24.323732844278712
49	29.223146999236395	23.459933631953167	22.65013846093496	24.666780907875477
50	26.074897205032787	24.714561654417196	22.474499975962157	26.73604116458786
51	25.555532287839476	25.52235121385217	22.525554588470623	26.39656190983773
52	27.546632681381762	24.419235348786177	22.67597545721308	25.358156512618983
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	147.0
6	294.0
7	335.5
8	377.0
9	487.5
10	598.0
11	7195.0
12	13792.0
13	12648.5
14	10507.5
15	9510.0
16	8582.5
17	7655.0
18	8264.0
19	8873.0
20	9625.0
21	10377.0
22	12080.5
23	13784.0
24	16614.0
25	19444.0
26	23664.5
27	27885.0
28	32717.0
29	37549.0
30	43350.0
31	49151.0
32	53258.0
33	57365.0
34	60908.5
35	64452.0
36	65854.0
37	67256.0
38	67592.5
39	69103.0
40	70277.0
41	72772.5
42	75268.0
43	77357.0
44	79446.0
45	83416.5
46	87387.0
47	93571.5
48	99756.0
49	102892.5
50	106029.0
51	114975.0
52	123921.0
53	149711.5
54	175502.0
55	194114.0
56	212726.0
57	239697.0
58	266668.0
59	292090.5
60	317513.0
61	300271.5
62	283030.0
63	288915.0
64	257690.5
65	220581.0
66	196342.0
67	172103.0
68	135827.0
69	99551.0
70	87385.0
71	75219.0
72	67859.5
73	60500.0
74	60147.5
75	59795.0
76	43656.5
77	27518.0
78	18406.5
79	9295.0
80	6538.5
81	3782.0
82	2537.5
83	1293.0
84	899.0
85	505.0
86	331.5
87	158.0
88	100.5
89	32.5
90	22.0
91	12.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3390487.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.064863233613746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.5648214640976	13.065682067908796
2	8.50741082053196	2.9035560424908717
3	3.1163029499418395	1.5953785090579364
4	1.5215979558706314	1.0386344405391426
5	0.9281105857564335	0.7919040105801338
6	0.6305462765996106	0.6456111583582042
7	0.45328027593923587	0.5414616140778431
8	0.3686670733667957	0.503300254859281
9	0.3193540362491586	0.49047596565249757
>10	5.504445778361607	24.29993630021033
>50	1.3672897328520306	15.88463196176392
>100	0.646811245146733	20.77578390664866
>500	0.04652509854462417	5.394404781191938
>1k	0.020988766260732712	6.337527207082775
>5k	0.002448689397085483	2.825785573415695
>10k+	0.0013992510840488473	2.9059262061619737
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGACATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	15169	0.44739885450084305	No Hit
GGACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	14028	0.41374587190571743	No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAG	12031	0.3548457787922502	No Hit
GGACATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	11468	0.3382404946546027	No Hit
GGACATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	11386	0.33582196303952794	No Hit
GGACATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	11334	0.33428826006411466	No Hit
GGACATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	11105	0.3275340681146986	No Hit
GGACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	10838	0.3196590932217112	No Hit
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9632	0.2840889819073189	No Hit
GGACATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	9523	0.2808741045165488	No Hit
GGACATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	9246	0.2727041867436743	No Hit
GGACATGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	8140	0.24008350422815364	No Hit
GGACATGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	6403	0.1888519259917528	No Hit
GGACATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	6377	0.18808507450404618	No Hit
GGACATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	6291	0.18554856573701656	No Hit
GGACATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	6183	0.18236318263423512	No Hit
GGACATGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	5922	0.1746651734691801	No Hit
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5497	0.16213010107397552	No Hit
GGACATGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	5453	0.16083235240247198	No Hit
GGACATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	5407	0.159475615154991	No Hit
GGACATGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	5395	0.1591216836991264	No Hit
GGACATGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	5205	0.15351776898127023	No Hit
GGACATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	4706	0.13880011927490063	No Hit
GGACATGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	4615	0.1361161390679274	No Hit
GGACATGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA	4576	0.13496586183636747	No Hit
GGACATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	4532	0.13366811316486393	No Hit
GGACATGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	3971	0.11712181760319387	No Hit
GGACATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	3941	0.11623698896353239	No Hit
GGACATGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	3822	0.11272716869287508	No Hit
GGACATGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	3759	0.11086902854958594	No Hit
GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	3744	0.11042661422975521	No Hit
GGACATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	3713	0.10951229130210499	No Hit
GGACATGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA	3474	0.1024631564728017	Illumina Single End Adapter 2 (97% over 34bp)
GGACATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	3434	0.10128338495325304	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.002624991630995783	0.0	0.0	0.0	0.0
8	0.006252789053607933	0.0	0.0	0.0	0.0
9	0.011178335147723616	0.0	0.0	0.0	0.0
10	0.06273435055200034	0.0	0.0	0.0	0.0
11	0.14390263109694862	0.0	0.0	0.0	0.0
12	0.21085466483133544	0.0	0.0	0.0	0.0
13	0.26241068023561215	0.0	0.0	0.0	0.0
14	0.3201310018295307	0.0	0.0	0.0	0.0
15	0.375580263248318	0.0	0.0	0.0	0.0
16	0.4317078932908458	0.0	0.0	0.0	0.0
17	0.4828214943752918	0.0	0.0	0.0	0.0
18	0.532253921044381	0.0	0.0	0.0	0.0
19	0.5896498054704236	0.0	0.0	0.0	0.0
20	0.6537703875578936	0.0	0.0	0.0	0.0
21	0.7124345263674511	0.0	0.0	0.0	0.0
22	0.7769680284867632	0.0	0.0	0.0	0.0
23	0.8533582343775393	0.0	0.0	0.0	0.0
24	0.927093954349331	0.0	0.0	0.0	0.0
25	0.9927482394122142	0.0	0.0	0.0	0.0
26	1.0539193927008126	0.0	0.0	0.0	0.0
27	1.1194851948997298	0.0	0.0	0.0	0.0
28	1.2296758548255753	0.0	0.0	0.0	0.0
29	1.2781054757030479	0.0	0.0	0.0	0.0
30	1.3388637089598043	0.0	0.0	0.0	0.0
31	1.3699801827879003	0.0	0.0	0.0	0.0
32	1.3989730678808088	0.0	0.0	0.0	0.0
33	1.4240137183832293	0.0	0.0	0.0	0.0
34	1.456427940882829	0.0	0.0	0.0	0.0
35	1.5211089144420846	0.0	0.0	0.0	0.0
36	1.5489220280154443	0.0	0.0	0.0	0.0
37	1.5734612756220567	0.0	0.0	0.0	0.0
38	1.5969682231490638	0.0	0.0	0.0	0.0
39	1.622451287971315	0.0	0.0	0.0	0.0
40	1.6486717099932842	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCGAT	35	1.0203621E-7	46.000004	41
ATCGTTA	25	3.418576E-5	46.0	15
ATGGGAT	18755	0.0	44.45481	5
ATGGGGC	40800	0.0	43.50833	5
ATGGGAG	33500	0.0	43.466568	5
TGGGATT	6410	0.0	43.27301	6
ATGGGAC	13005	0.0	43.188004	5
ATGGGCT	8675	0.0	42.95101	5
CATGGGG	209015	0.0	42.897976	4
ACATGGG	350580	0.0	42.709877	3
ATGGGGT	32095	0.0	42.653374	5
GGACATG	364045	0.0	42.63319	1
GACATGG	360925	0.0	42.60345	2
ATGGGGG	78085	0.0	42.580265	5
TGGGGCT	19380	0.0	42.47523	6
TATAGTC	65	0.0	42.461536	19
TGGGATC	6090	0.0	42.147785	6
CATGGGA	97750	0.0	42.143528	4
TGGGGAC	10920	0.0	41.99817	6
ATGGGGA	59465	0.0	41.996803	5
>>END_MODULE