SRR1527547_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527547_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3872939
Sequences flagged as poor quality	0
Sequence length	52
%GC	55

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GGACATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	18663	0.48188210555343114	No Hit
GGACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	15717	0.405815841664431	No Hit
GGACATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	13893	0.35871982491849214	No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAG	13852	0.3576611973490933	No Hit
GGACATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	13411	0.3462744959319008	No Hit
GGACATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	13122	0.3388124625768699	No Hit
GGACATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	12896	0.3329771008528665	No Hit
GGACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	12247	0.3162198010348214	No Hit
GGACATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	10988	0.2837121885988909	No Hit
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10535	0.2720156449662647	No Hit
GGACATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	9987	0.2578661837947874	No Hit
GGACATGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	9818	0.25350257259409453	No Hit
GGACATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	7603	0.19631086366193734	No Hit
GGACATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	7204	0.1860086099987632	No Hit
GGACATGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	7122	0.18389135485996552	No Hit
GGACATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	7036	0.18167081898268989	No Hit
GGACATGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	6479	0.16728897614963725	No Hit
GGACATGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	6307	0.162847904395086	No Hit
GGACATGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	6246	0.16127287313329747	No Hit
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6101	0.15752894636347228	No Hit
GGACATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	6050	0.1562121169478786	No Hit
GGACATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	5740	0.14820785971583855	No Hit
GGACATGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	5562	0.14361186685357036	No Hit
GGACATGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	5539	0.14301800260732225	No Hit
GGACATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	5372	0.1387060317758684	No Hit
GGACATGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA	5236	0.13519448666761857	No Hit
GGACATGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	4747	0.12256841638869087	No Hit
GGACATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	4639	0.11977983644978658	No Hit
GGACATGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	4578	0.11820480518799806	No Hit
GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	4381	0.1131182288179597	No Hit
GGACATGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	4274	0.11035546906367491	No Hit
GGACATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	3989	0.10299671644712194	No Hit
GGACATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	3976	0.10266105404706866	No Hit
GGACATGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	3884	0.1002855970620761	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAATT	25	3.418667E-5	46.0	44
ATGGGAT	22680	0.0	44.276012	5
ATGGGGC	47110	0.0	43.466145	5
ATGGGCT	10190	0.0	43.133465	5
ATGGGAG	38115	0.0	43.109535	5
ATGGGAC	14785	0.0	43.075417	5
ATGGGGT	36005	0.0	43.029575	5
CATGGGG	237975	0.0	42.985523	4
CGTGTAA	75	0.0	42.933334	30
TGGGATT	7795	0.0	42.84285	6
ACATGGG	399700	0.0	42.786217	3
GGACATG	414840	0.0	42.72165	1
GACATGG	411105	0.0	42.67844	2
ATGGGGG	87970	0.0	42.50438	5
TGGGATA	3200	0.0	42.334373	6
ATGGGGA	68435	0.0	42.279533	5
CATGGGA	112770	0.0	42.257427	4
TGGGGCT	21990	0.0	42.077763	6
ATGGGCG	4760	0.0	41.892857	5
TGGGATC	7350	0.0	41.681633	6

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content