##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527547_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3872939
Sequences flagged as poor quality	0
Sequence length	52
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.378102521108648	31.0	31.0	33.0	26.0	34.0
2	31.42361653514295	31.0	31.0	34.0	28.0	34.0
3	31.256110153038815	31.0	31.0	34.0	28.0	34.0
4	35.83627214371308	37.0	35.0	37.0	35.0	37.0
5	34.757545884404585	35.0	35.0	37.0	32.0	37.0
6	35.61596477507133	37.0	35.0	37.0	35.0	37.0
7	36.23791027950608	37.0	35.0	37.0	35.0	37.0
8	36.426633365513894	37.0	37.0	37.0	35.0	37.0
9	38.45539782578553	39.0	39.0	39.0	37.0	39.0
10	36.88261601848105	39.0	37.0	39.0	32.0	39.0
11	36.80922988975556	39.0	37.0	39.0	32.0	39.0
12	36.65493621252491	39.0	35.0	39.0	32.0	39.0
13	36.61089911305084	39.0	35.0	39.0	32.0	39.0
14	37.39822754760661	40.0	37.0	40.0	33.0	40.0
15	37.59880726239169	40.0	37.0	40.0	33.0	40.0
16	37.57874291332758	40.0	37.0	40.0	33.0	40.0
17	37.61747293205496	40.0	37.0	40.0	33.0	40.0
18	37.55632479623356	40.0	37.0	40.0	33.0	40.0
19	37.360935971364384	40.0	36.0	40.0	32.0	40.0
20	37.321495381156275	39.0	36.0	40.0	32.0	40.0
21	37.39775503822808	39.0	36.0	40.0	32.0	40.0
22	37.38475638268509	39.0	36.0	40.0	32.0	40.0
23	37.29332401052534	39.0	36.0	40.0	32.0	40.0
24	37.21221274076354	39.0	36.0	40.0	32.0	40.0
25	37.04712364434348	39.0	36.0	40.0	32.0	40.0
26	36.771150797882434	39.0	35.0	40.0	32.0	40.0
27	36.598482186267326	38.0	35.0	40.0	31.0	40.0
28	36.35947738913523	38.0	35.0	40.0	31.0	40.0
29	36.29945475516139	38.0	35.0	40.0	31.0	40.0
30	36.08277460605499	38.0	35.0	40.0	30.0	40.0
31	35.96089687960487	38.0	35.0	40.0	30.0	40.0
32	35.74228951191847	38.0	35.0	40.0	30.0	40.0
33	35.559151848247545	38.0	35.0	40.0	29.0	40.0
34	35.15231404367587	38.0	34.0	40.0	26.0	40.0
35	34.949423680569204	38.0	34.0	40.0	26.0	40.0
36	34.75004899380031	38.0	34.0	40.0	25.0	40.0
37	34.39277096799098	37.0	33.0	40.0	23.0	40.0
38	34.129019073112175	37.0	33.0	40.0	22.0	40.0
39	33.764295280664115	37.0	33.0	40.0	21.0	40.0
40	33.66873400278187	37.0	33.0	40.0	18.0	40.0
41	33.63443008010196	37.0	33.0	40.0	17.0	40.0
42	33.57264341111492	37.0	33.0	40.0	16.0	40.0
43	33.447669844528924	37.0	33.0	40.0	15.0	40.0
44	33.28203697502078	37.0	33.0	40.0	15.0	40.0
45	33.19015378243758	36.0	33.0	40.0	15.0	40.0
46	32.89278658920267	36.0	33.0	40.0	13.0	40.0
47	32.81585483272522	35.0	33.0	40.0	13.0	40.0
48	32.66362780307152	35.0	32.0	39.0	13.0	40.0
49	32.46407366601953	35.0	32.0	39.0	12.0	40.0
50	32.23743802832939	35.0	32.0	39.0	11.0	40.0
51	32.078389305899215	35.0	32.0	39.0	10.0	40.0
52	31.577033875307617	35.0	31.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	6.0
11	30.0
12	43.0
13	93.0
14	141.0
15	357.0
16	728.0
17	1459.0
18	2766.0
19	4870.0
20	7956.0
21	12053.0
22	18008.0
23	25775.0
24	35908.0
25	49266.0
26	66510.0
27	88901.0
28	107075.0
29	108534.0
30	99153.0
31	104323.0
32	127663.0
33	171775.0
34	278606.0
35	292234.0
36	450558.0
37	692561.0
38	983261.0
39	142324.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	100.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.47804496791713	0.2920262880463648	0.1489308248851841	0.08099791915132151
8	98.87886176363739	0.7084025852201649	0.27586285247456777	0.13687279866788504
9	96.80937396638573	1.645287984138144	0.7394900875020236	0.8058479619740978
10	58.99641073613604	27.858249252053803	5.516095141183479	7.629244870626674
11	34.69272301990814	29.95174982100157	17.73616883715442	17.619358321935874
12	30.018494998242932	24.270870261576544	23.273281608618156	22.43735313156236
13	22.874643778277942	25.22438385938947	25.100085490631272	26.800886871701312
14	23.911608212781044	27.67624277067106	25.636267444439483	22.775881572108418
15	26.46661876161747	25.115241939002914	23.830507012891246	24.587632286488375
16	27.335855276832397	26.06547637336917	23.41376923313277	23.184899116665665
17	30.07829454582166	26.213064548654135	19.70854692005219	24.000093985472017
18	28.907555734805012	25.817964083606793	20.269671172202816	25.004809009385383
19	27.701417450675052	26.742946377415187	21.58577762262716	23.9698585492826
20	26.961643341142217	29.61045862070123	20.284646879282118	23.14325115887444
21	28.453043024948236	26.32902299778024	23.11002058126916	22.107913396002363
22	28.261353974333186	26.65319541567786	20.444448001892106	24.641002608096848
23	27.827987995679766	28.957621072782196	19.685954258510137	23.5284366730279
24	27.284059986485715	28.78516805970866	19.58894782489474	24.341824128910886
25	26.473332009618534	26.31799777894772	19.989625449819894	27.21904476161385
26	28.023782455649314	26.28786562349678	19.626593654069946	26.061758266783958
27	26.56718838071036	25.118133799680294	19.798943386405004	28.515734433204344
28	28.72906079853052	29.077116887201164	17.96183725072871	24.2319850635396
29	30.123092566136467	27.391394493948912	18.852891821947104	23.632621117967517
30	27.889620776366474	28.30318267341675	19.335987476177653	24.47120907403912
31	31.14794733405303	27.242412028694485	18.464969368223976	23.144671269028507
32	31.240848358313933	26.312704641100726	20.333885971351474	22.11256102923387
33	32.24352358764236	26.64142141149138	19.128289911098523	21.986765089767747
34	27.834055739065345	29.382647131803523	20.96307223015906	21.820224898972075
35	27.9484907972989	31.697684884786465	20.425831648781458	19.927992669133182
36	30.061020842311226	30.08077328354513	17.466089706034616	22.392116168109023
37	29.093099581480626	29.926368579520616	19.138592164761697	21.84193967423706
38	31.850876040133862	28.247617636115624	18.62562255692641	21.275883766824112
39	30.784760617195367	25.8705856198613	18.49220966299753	24.852444099945803
40	29.250292865444045	26.142859466673755	20.227558451088438	24.379289216793758
41	29.020028459007484	25.343234169192957	19.76648741433831	25.870249957461244
42	29.26235089166134	23.525493171981278	20.7279019886448	26.484253947712578
43	27.637848156141885	23.85828953154181	22.592016037433073	25.91184627488323
44	27.81634309241638	22.925148059393656	22.16621537287316	27.092293475316808
45	27.971367480871763	24.52434701398602	21.750871883084137	25.75341362205808
46	27.935580704989153	24.251195280896496	22.86640713938433	24.94681687473002
47	28.70659723791157	24.92610908666519	21.496930367351514	24.870363308071724
48	29.121734166223632	23.974428721960248	22.43990932983969	24.463927781976427
49	28.93192998908581	23.549609224415878	22.738442304410164	24.780018482088153
50	26.02953467637884	24.663724370561997	22.501258088495586	26.80548286456358
51	25.654057551642307	25.458082350380423	22.347111586317265	26.540748511660006
52	27.59483172856583	24.402682304059013	22.494699761602234	25.50778620577293
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	141.0
6	282.0
7	321.0
8	360.0
9	527.5
10	695.0
11	7800.5
12	14906.0
13	13813.5
14	11652.0
15	10583.0
16	9819.0
17	9055.0
18	9886.5
19	10718.0
20	11843.5
21	12969.0
22	15239.0
23	17509.0
24	21380.5
25	25252.0
26	30495.5
27	35739.0
28	40924.0
29	46109.0
30	50648.5
31	55188.0
32	57756.5
33	60325.0
34	62931.5
35	65538.0
36	66551.0
37	67564.0
38	67881.0
39	69679.5
40	71161.0
41	76103.5
42	81046.0
43	83744.5
44	86443.0
45	90926.5
46	95410.0
47	104759.5
48	114109.0
49	118446.0
50	122783.0
51	133443.0
52	144103.0
53	173297.5
54	202492.0
55	224979.0
56	247466.0
57	278878.5
58	310291.0
59	339650.5
60	369010.0
61	349361.5
62	329713.0
63	334198.0
64	297404.5
65	256126.0
66	228638.0
67	201150.0
68	158375.0
69	115600.0
70	101254.5
71	86909.0
72	78485.0
73	70061.0
74	69283.5
75	68506.0
76	50017.0
77	31528.0
78	20939.5
79	10351.0
80	7341.0
81	4331.0
82	2852.0
83	1373.0
84	896.0
85	419.0
86	261.0
87	103.0
88	72.0
89	29.0
90	17.0
91	9.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3872939.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.93039256423434
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.89902758587503	14.146905375510938
2	8.26843282762354	2.965124929805844
3	2.782989375564703	1.4970027601790559
4	1.384231194270261	0.9927923485169884
5	0.833263574591284	0.7470371500949442
6	0.5373529139585828	0.5780969215687576
7	0.39499069141174087	0.4957636709361602
8	0.3366390193729524	0.482885581583674
9	0.25039019345386565	0.4040635016576153
>10	4.184224395007291	19.92331893644684
>50	1.399752742125094	17.157049597782137
>100	0.660122066721704	22.40075415925573
>500	0.0442753724205124	5.526821509441798
>1k	0.020546087855270463	6.385192160148693
>5k	0.0023150521527065307	2.786396803095629
>10k+	0.0014469075954415818	3.5107945939750924
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGACATGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	18663	0.48188210555343114	No Hit
GGACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	15717	0.405815841664431	No Hit
GGACATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	13893	0.35871982491849214	No Hit
GGACATGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGGACATGGGAAG	13852	0.3576611973490933	No Hit
GGACATGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	13411	0.3462744959319008	No Hit
GGACATGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	13122	0.3388124625768699	No Hit
GGACATGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	12896	0.3329771008528665	No Hit
GGACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	12247	0.3162198010348214	No Hit
GGACATGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	10988	0.2837121885988909	No Hit
GGACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10535	0.2720156449662647	No Hit
GGACATGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	9987	0.2578661837947874	No Hit
GGACATGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	9818	0.25350257259409453	No Hit
GGACATGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	7603	0.19631086366193734	No Hit
GGACATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	7204	0.1860086099987632	No Hit
GGACATGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	7122	0.18389135485996552	No Hit
GGACATGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	7036	0.18167081898268989	No Hit
GGACATGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	6479	0.16728897614963725	No Hit
GGACATGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	6307	0.162847904395086	No Hit
GGACATGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	6246	0.16127287313329747	No Hit
GGACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6101	0.15752894636347228	No Hit
GGACATGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	6050	0.1562121169478786	No Hit
GGACATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	5740	0.14820785971583855	No Hit
GGACATGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	5562	0.14361186685357036	No Hit
GGACATGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	5539	0.14301800260732225	No Hit
GGACATGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	5372	0.1387060317758684	No Hit
GGACATGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA	5236	0.13519448666761857	No Hit
GGACATGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	4747	0.12256841638869087	No Hit
GGACATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	4639	0.11977983644978658	No Hit
GGACATGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	4578	0.11820480518799806	No Hit
GGACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	4381	0.1131182288179597	No Hit
GGACATGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC	4274	0.11035546906367491	No Hit
GGACATGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTT	3989	0.10299671644712194	No Hit
GGACATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	3976	0.10266105404706866	No Hit
GGACATGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	3884	0.1002855970620761	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0017041321848859485	0.0	0.0	0.0	0.0
8	0.004725093785365584	0.0	0.0	0.0	0.0
9	0.008804682955244067	0.0	0.0	0.0	0.0
10	0.051614549054348646	0.0	0.0	0.0	0.0
11	0.11546786561833275	0.0	0.0	0.0	0.0
12	0.1691738496268596	0.0	0.0	0.0	0.0
13	0.2122677377567785	0.0	0.0	0.0	0.0
14	0.25858914896413293	0.0	0.0	0.0	0.0
15	0.3032064279866014	0.0	0.0	0.0	0.0
16	0.35074138787107156	0.0	0.0	0.0	0.0
17	0.39207950344686554	0.0	0.0	0.0	0.0
18	0.4338565621611908	0.0	0.0	0.0	0.0
19	0.482037026661148	0.0	0.0	0.0	0.0
20	0.5360786730697282	0.0	0.0	0.0	0.0
21	0.5849046421851726	0.0	0.0	0.0	0.0
22	0.6441103255176495	0.0	0.0	0.0	0.0
23	0.7144703286057436	0.0	0.0	0.0	0.0
24	0.7843913885553064	0.0	0.0	0.0	0.0
25	0.8501295785965128	0.0	0.0	0.0	0.0
26	0.9134664914681073	0.0	0.0	0.0	0.0
27	0.9826387660637051	0.0	0.0	0.0	0.0
28	1.089637611126847	0.0	0.0	0.0	0.0
29	1.139289826150115	0.0	0.0	0.0	0.0
30	1.20058694443677	0.0	0.0	0.0	0.0
31	1.2336109605650902	2.5820184619484065E-5	0.0	0.0	0.0
32	1.2635107343544527	2.5820184619484065E-5	0.0	0.0	0.0
33	1.290931770420345	2.5820184619484065E-5	0.0	0.0	0.0
34	1.3210381056866634	2.5820184619484065E-5	0.0	0.0	0.0
35	1.3870861379433035	2.5820184619484065E-5	0.0	0.0	0.0
36	1.4158498236094088	2.5820184619484065E-5	0.0	0.0	0.0
37	1.4411536045365032	2.5820184619484065E-5	0.0	0.0	0.0
38	1.4645208716171363	2.5820184619484065E-5	0.0	0.0	0.0
39	1.487655757036194	2.5820184619484065E-5	0.0	0.0	0.0
40	1.5133726609172	2.5820184619484065E-5	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAATT	25	3.418667E-5	46.0	44
ATGGGAT	22680	0.0	44.276012	5
ATGGGGC	47110	0.0	43.466145	5
ATGGGCT	10190	0.0	43.133465	5
ATGGGAG	38115	0.0	43.109535	5
ATGGGAC	14785	0.0	43.075417	5
ATGGGGT	36005	0.0	43.029575	5
CATGGGG	237975	0.0	42.985523	4
CGTGTAA	75	0.0	42.933334	30
TGGGATT	7795	0.0	42.84285	6
ACATGGG	399700	0.0	42.786217	3
GGACATG	414840	0.0	42.72165	1
GACATGG	411105	0.0	42.67844	2
ATGGGGG	87970	0.0	42.50438	5
TGGGATA	3200	0.0	42.334373	6
ATGGGGA	68435	0.0	42.279533	5
CATGGGA	112770	0.0	42.257427	4
TGGGGCT	21990	0.0	42.077763	6
ATGGGCG	4760	0.0	41.892857	5
TGGGATC	7350	0.0	41.681633	6
>>END_MODULE