FastQCFastQC Report
Fri 17 Jun 2016
SRR1527546_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527546_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences851075
Sequences flagged as poor quality0
Sequence length50
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT70540.8288341215521546No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC60120.7064007284904386No Hit
CGCACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG42820.5031283964397967No Hit
CGCACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC41450.48703110771671126No Hit
CGCACCGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGA39590.4651763945598214No Hit
CGCACCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG38790.4557765179332022No Hit
CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC37240.4375642569691273No Hit
CGCACCGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGG34670.40736715330611284No Hit
CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT28610.3361630878594718No Hit
CGCACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT25850.30373351349763533No Hit
CGCACCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC22320.2622565578826778No Hit
CGCACCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTT19140.22489204829186618No Hit
CGCACCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC17090.20080486443615425No Hit
CGCACCGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCT16540.19434244925535354No Hit
CGCACCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA16380.1924624739300297No Hit
CGCACCGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT15930.18717504332755633No Hit
CGCACCGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTG15700.18447257879740328No Hit
CGCACCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT15500.18212260964074847No Hit
CGCACCGGGGCGGATCTGCGGCGGCGACAGTTGGAGAGGGACGTTCCCGG15050.17683517903827511No Hit
CGCACCGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTA15000.1762476867491114No Hit
CGCACCGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTC14820.1741327145081221No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGGGA14800.1738977175924566No Hit
CGCACCGGGGGGCAGTGGTGGCTCATGCCTTTAATCCCAGCACTTGGGAG14480.17013776694180888No Hit
CGCACCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC13540.15909291190553124No Hit
CGCACCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC13160.15462797050788707No Hit
CGCACCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC13000.1527479951825632No Hit
CGCACCGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT12820.1506330229415739No Hit
CGCACCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA12460.14640307845959522No Hit
CGCACCGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCC12200.14334811855594395No Hit
CGCACCGGGGTCGGGCGGTGGTGTGCAGTGCTCGCACCGGGGTCGGGCGG11960.14052815556795817No Hit
CGCACCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG11910.13994066327879445No Hit
CGCACCGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAAC11600.13629821108597948No Hit
CGCACCGGGAGCTCTTTCTCTCGGGTACACACAGCTCCGCACATTCGCAC11350.13336074964016098No Hit
CGCACCGGTGCTGCCTGTTGACTCTGGAGGCATTGCAGTCCAGACTGAAC11270.13242076197749905No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC11130.13077578356784067No Hit
CGCACCGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGG10890.12795582057985488No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGCGA10870.1277208236641894No Hit
CGCACCGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGT10770.12654583908586198No Hit
CGCACCGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAG10740.12619334371236376No Hit
CGCACCGGGGGGCGTGGTGGCTCACGCCTTTAATCCCAGCACTCGTGAGG10670.12537085450753457No Hit
CGCACCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCC10590.12443086684487267No Hit
CGCACCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC10420.12243339306171605No Hit
CGCACCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGT10400.12219839614605059No Hit
CGCACCGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAA10260.12055341773639221No Hit
CGCACCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC10260.12055341773639221No Hit
CGCACCGGGGATCCTCTGTGATTGTGATGAGTTCAGGAAGATCAAGCCAA9900.11632347325441353No Hit
CGCACCGGGTGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAG9880.11608847633874804No Hit
CGCACCGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAA9800.11514848867608612No Hit
CGCACCGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCA9770.1147959933025879No Hit
CGCACCGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATC9530.11197603031460211No Hit
CGCACCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT9470.11127103956760569No Hit
CGCACCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG9330.1096260611579473No Hit
CGCACCGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCT9270.10892107041095085No Hit
CGCACCGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT9090.10680609816996153No Hit
CGCACCGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTC9030.10610110742296508No Hit
CGCACCGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGAC8780.10316364597714656No Hit
CGCACCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA8550.10046118144699352No Hit
CGCACCGGGGCAGACGTGGGCACCAGCCCCCACGTCCGCTTGCCCGCCCT8520.1001086860734953No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG207.855328E-444.00000412
TGATAAT207.855328E-444.00000438
GTATTAT207.855328E-444.00000421
GGTCGAA408.307325E-944.00000426
TACCACG207.855328E-444.00000413
AACCCTA207.855328E-444.00000437
TTAGATC207.855328E-444.00000442
TATGGAT650.044.00000442
TGAGTTA207.855328E-444.00000431
TACGCTT207.855328E-444.00000441
CCCAATC207.855328E-444.00000440
CCGTAAT207.855328E-444.00000425
TACGCCC207.855328E-444.00000443
CTAGTAC207.855328E-444.00000439
CGTAGAA207.855328E-444.00000436
ACTACGT207.855328E-444.00000441
ACCACGC207.855328E-444.00000438
CTCGATG207.855328E-444.00000411
CGTAATG207.855328E-444.00000423
GGTGATA650.044.00000417
Produced by FastQC (version 0.11.5)