FastQCFastQC Report
Fri 17 Jun 2016
SRR1527545_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527545_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673011
Sequences flagged as poor quality0
Sequence length52
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG57830.8592727310549159No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC46970.6979083551383262No Hit
CGCACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC34670.51514759788473No Hit
CGCACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT34630.514553253958702No Hit
CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA34220.5084612287169155No Hit
CGCACCGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAAA31400.4665599819319447No Hit
CGCACCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT31030.461062300616186No Hit
CGCACCGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA27980.41574357625655445No Hit
CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT22820.3390732097989483No Hit
CGCACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC19910.2958346891804146No Hit
CGCACCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC17770.26403728913791896No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGGGAAG15180.22555351992760891No Hit
CGCACCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA15000.22287897226048312No Hit
CGCACCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT14180.21069492177691004No Hit
CGCACCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG13970.20757461616526326No Hit
CGCACCGGGGCGGATCTGCGGCGGCGACAGTTGGAGAGGGACGTTCCCGGGA13850.2057915843871794No Hit
CGCACCGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCT13570.2016311769049837No Hit
CGCACCGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT13100.19464763577415525No Hit
CGCACCGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG13050.1939047058666203No Hit
CGCACCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT13040.19375611988511332No Hit
CGCACCGGGGGGCAGTGGTGGCTCATGCCTTTAATCCCAGCACTTGGGAGGC12580.18692116473579184No Hit
CGCACCGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGAA12310.18290934323510313No Hit
CGCACCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA11260.16730781517686932No Hit
CGCACCGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCG11250.16715922919536233No Hit
CGCACCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT10860.16136437591658978No Hit
CGCACCGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC10800.16047286002754785No Hit
CGCACCGGGGTCGGGCGGTGGTGTGCAGTGCTCGCACCGGGGTCGGGCGGTG10500.15601528058233818No Hit
CGCACCGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG10380.15423224880425432No Hit
CGCACCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT10250.15230063104466346No Hit
CGCACCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA10000.14858598150698873No Hit
CGCACCGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTC9560.14204819832068122No Hit
CGCACCGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACAG9420.1399679945795834No Hit
CGCACCGGTGCTGCCTGTTGACTCTGGAGGCATTGCAGTCCAGACTGAACCC9360.13907647869054146No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC9320.13848213476451351No Hit
CGCACCGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGAT9080.13491607120834578No Hit
CGCACCGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA8720.1295669758740942No Hit
CGCACCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG8710.1294183898925872No Hit
CGCACCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT8670.12882404596655925No Hit
CGCACCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG8600.12778394409601032No Hit
CGCACCGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATG8450.12555515437340548No Hit
CGCACCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGC8400.12481222446587054No Hit
CGCACCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG8350.1240692945583356No Hit
CGCACCGGGAGCTCTTTCTCTCGGGTACACACAGCTCCGCACATTCGCACCC8200.12184050483573076No Hit
CGCACCGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAAT7970.11842302726107001No Hit
CGCACCGGGGGGCGTGGTGGCTCACGCCTTTAATCCCAGCACTCGTGAGGCA7830.11634282351997219No Hit
CGCACCGGGGATCCTCTGTGATTGTGATGAGTTCAGGAAGATCAAGCCAAAG7690.11426261977887434No Hit
CGCACCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG7680.11411403379736734No Hit
CGCACCGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAATT7510.11158807211174855No Hit
CGCACCGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC7480.11114231416722757No Hit
CGCACCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA7470.11099372818572058No Hit
CGCACCGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT7410.11010221229667866No Hit
CGCACCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG7400.10995362631517167No Hit
CGCACCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA7210.10713049266653889No Hit
CGCACCGGGGCAGACGTGGGCACCAGCCCCCACGTCCGCTTGCCCGCCCTAC7200.1069819066850319No Hit
CGCACCGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAA7120.10579321883297599No Hit
CGCACCGGGTGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGA7110.105644632851469No Hit
CGCACCGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCAT7040.10460453098092007No Hit
CGCACCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT7030.1044559449994131No Hit
CGCACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT7010.10415877303639912No Hit
CGCACCGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATG6760.10044412349872439No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGA301.8602459E-646.022
ACTATTA301.8602459E-646.034
CTAGACG253.4156357E-546.014
TAATACA206.30958E-446.027
ATCTCGA501.6370905E-1146.043
ACCGGTT453.092282E-1046.04
GTACCAC206.30958E-446.025
TACCCGA600.046.019
CGAACAA206.30958E-446.028
TCATTGG253.4156357E-546.044
TAGACGG253.4156357E-546.015
GCGAAAT206.30958E-446.09
CACATCG206.30958E-446.012
TATGGAT405.6061253E-946.042
GTTAGTT301.8602459E-646.036
CAATTCG253.4156357E-546.030
TACGGTC405.6061253E-946.019
GGATATA405.6061253E-946.08
CTAAGGT206.30958E-446.021
GAATAGT206.30958E-446.024
Produced by FastQC (version 0.11.5)