FastQCFastQC Report
Fri 17 Jun 2016
SRR1527544_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527544_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906834
Sequences flagged as poor quality0
Sequence length52
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCACCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG79570.8774483532818576No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC63500.7002384118813366No Hit
CGCACCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC47160.5200510788082493No Hit
CGCACCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT45640.5032894664293576No Hit
CGCACCGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAAA43620.4810141657679355No Hit
CGCACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA42650.47031761049982684No Hit
CGCACCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT41670.45951078146606766No Hit
CGCACCGGGGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGAA40090.4420875264932722No Hit
CGCACCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT29610.326520620091439No Hit
CGCACCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC25740.2838446727846552No Hit
CGCACCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC24770.2731481175165466No Hit
CGCACCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA20230.22308382791117226No Hit
CGCACCGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG18870.2080865957826901No Hit
CGCACCGGGGCGGATCTGCGGCGGCGACAGTTGGAGAGGGACGTTCCCGGGA18140.20003661089019598No Hit
CGCACCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT17990.19838250440543692No Hit
CGCACCGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG17990.19838250440543692No Hit
CGCACCGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTCGCACCGGGAAG17910.1975003142802321No Hit
CGCACCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT17880.19716949298328026No Hit
CGCACCGGGGGGCTGGCGAGATGGCTCAGTGGTTAAGAGCGCCGACTGCTCT16910.18647293771517168No Hit
CGCACCGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT16860.1859215688869187No Hit
CGCACCGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGAA16540.18239280838609934No Hit
CGCACCGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCG16020.1766585725722679No Hit
CGCACCGGGGGGCAGTGGTGGCTCATGCCTTTAATCCCAGCACTTGGGAGGC15390.16971132533627986No Hit
CGCACCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA14510.1600072339590267No Hit
CGCACCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT14450.15934559136512305No Hit
CGCACCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT14160.15614765216125553No Hit
CGCACCGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC13700.15107505894132775No Hit
CGCACCGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG13120.14467918053359272No Hit
CGCACCGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGAT12950.14280452651753242No Hit
CGCACCGGGGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCTC12820.14137096756407458No Hit
CGCACCGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA12720.14026822990756854No Hit
CGCACCGGGGTCGGGCGGTGGTGTGCAGTGCTCGCACCGGGGTCGGGCGGTG12500.13784220706325523No Hit
CGCACCGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC12370.1364086481097974No Hit
CGCACCGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACAG11970.1319976974837732No Hit
CGCACCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG11960.13188742371812262No Hit
CGCACCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG11910.1313360548898696No Hit
CGCACCGGTGCTGCCTGTTGACTCTGGAGGCATTGCAGTCCAGACTGAACCC11830.13045386476466475No Hit
CGCACCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT11760.12968194840511052No Hit
CGCACCGGGAGCTCTTTCTCTCGGGTACACACAGCTCCGCACATTCGCACCC11660.12857921074860448No Hit
CGCACCGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA11640.1283586632173033No Hit
CGCACCGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAAT11380.12549154531038756No Hit
CGCACCGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC11260.12416826012258031No Hit
CGCACCGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATG11090.12229360610652004No Hit
CGCACCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGC11060.12196278480956822No Hit
CGCACCGGGGGGCGTGGTGGCTCACGCCTTTAATCCCAGCACTCGTGAGGCA10380.11446416874532714No Hit
CGCACCGGGGATCCTCTGTGATTGTGATGAGTTCAGGAAGATCAAGCCAAAG10330.11391279991707413No Hit
CGCACCGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG10270.11325115732317051No Hit
CGCACCGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAATT10220.11269978849491748No Hit
CGCACCGGGTGCTCTCAGCTCCTGAAGGCAAATCCCAATTCCAGAGCAAGGA10080.11115595577580901No Hit
CGCACCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG9990.1101634918849536No Hit
CGCACCGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCAG9980.11005321811930298No Hit
CGCACCGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC9940.10961212305670057No Hit
CGCACCGGGGCAGACGTGGGCACCAGCCCCCACGTCCGCTTGCCCGCCCTAC9910.10928130175974875No Hit
CGCACCGGGCTCTTTCCAGCCAGCGCCGAGCGATGGGCATCTCTCGGGACAA9860.10872993293149574No Hit
CGCACCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA9860.10872993293149574No Hit
CGCACCGGGGCTCTTGAGTCACCCCCGCGCAGCCTAGGCTTGCCGTGCGAGT9500.10476007736807398No Hit
CGCACCGGGGAAAGCGGAAACGCTTCGGGCCCCGGGCCGGTGTATCTGTTTC9480.10453952983677277No Hit
CGCACCGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG9480.10453952983677277No Hit
CGCACCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG9380.10343679218026672No Hit
CGCACCGGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACTGACTGCTCT9380.10343679218026672No Hit
CGCACCGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATG9110.10045940050770041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTG351.0192525E-746.00000423
GCATTAT351.0192525E-746.0000049
AACGATT351.0192525E-746.00000422
ATAGTTG700.046.00000436
TTACCAG351.0192525E-746.00000445
ACGCGAT206.310747E-446.024
ACAACGA501.6370905E-1146.022
AGCGTTA453.092282E-1046.045
ACTATTA900.046.034
CCTTAAC551.8189894E-1246.037
GGTACGA206.310747E-446.023
CAAACGT501.6370905E-1146.015
AATTTCG206.310747E-446.012
ACTGATT253.4165812E-546.041
TACCACG405.6097633E-946.013
CAAAACG253.4165812E-546.025
CGAAACC206.310747E-446.032
CATGCGG206.310747E-446.030
TAGACGG301.8609662E-646.015
GGCACGA206.310747E-446.038
Produced by FastQC (version 0.11.5)