FastQCFastQC Report
Fri 17 Jun 2016
SRR1527540_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527540_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416729
Sequences flagged as poor quality0
Sequence length50
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT48251.1578267891123488No Hit
GACACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC33890.8132383395444042No Hit
GACACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC24170.5799932330123414No Hit
GACACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG18440.44249380292708207No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC16010.3841825262940664No Hit
GACACTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG14760.354187013622762No Hit
GACACTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG13770.33043056758708894No Hit
GACACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC11330.27187932685270283No Hit
GACACTGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGT9390.22532629118683845No Hit
GACACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGACACTGGGA9160.21980711685531845No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC8960.21500783482790975No Hit
GACACTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT8860.21260819381420537No Hit
GACACTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA8550.20516930667172192No Hit
GACACTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG8460.203009629759388No Hit
GACACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG7990.19173131699497756No Hit
GACACTGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAA7040.16893472736478624No Hit
GACACTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG6890.1653352658442297No Hit
GACACTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT6880.16509530174285927No Hit
GACACTGGGTGCTGACACTGGGGCTGACACTGGGGGGGTTGGGGATTTAG6610.1586162710058575No Hit
GACACTGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT6530.15669655819489403No Hit
GACACTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT6350.15237720437022623No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG6020.14445838902500185No Hit
GACACTGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTA6020.14445838902500185No Hit
GACACTGGGGAAGCTCGAGCTGCCGCGTCCGCCCTGCGCCTGCTCTCCTG5980.1434985326195201No Hit
GACACTGGGGAGACTGAGAAGGAGGACAGGAGCGGGCTGGCGATCTGGGA5970.1432585685181497No Hit
GACACTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA5730.13749943008525925No Hit
GACACTGGGGGTTGGGGTCGGACCGCTGCAGGGGATGGTGCGATTTGGGC5710.13701950188251835No Hit
GACACTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC5660.1358196813756662No Hit
GACACTGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA5540.132940112159221No Hit
GACACTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT5520.1324601839564801No Hit
GACACTGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC5460.13102039934825752No Hit
GACACTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGT5400.1295806147400349No Hit
GACACTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA5300.12718097372633053No Hit
GACACTGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAA5260.12622111732084879No Hit
GACACTGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTC5170.1240614404085149No Hit
GACACTGGGGGCCACCAGCGGCAAACTAGGACTGTGCGTTTGCTTTCTCA5070.12166179939481053No Hit
GACACTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT5040.12094190709069923No Hit
GACACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA4990.11974208658384707No Hit
GACACTGGGCAGGGGTTCATTGCCTGGGACAAGGGCTCCAGAACCCACCT4840.11614262506329054No Hit
GACACTGGGGGCAGCCGCCGGAGCCGAGATGCCTAAAGGAGGTAGAAAGG4820.11566269686054965No Hit
GACACTGGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG4780.11470284045506793No Hit
GACACTGGGGTTCTCCCCAGGTTGGTTCGGCCCCCGTCTACTCTGGGGTG4730.11350301994821575No Hit
GACACTGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCC4570.10966359432628878No Hit
GACACTGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT4530.10870373792080705No Hit
GACACTGGGGGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCGTTC4520.10846377381943662No Hit
GACACTGGGCTGAGCCAAAACCACTAGTCCAAGAAAAAGAAGAAATTAAA4420.10606413280573226No Hit
GACACTGGGGGCAGGAAGCGGAAGAACCGTAGGGTGGGCGGCTCTTTGTC4400.10558420460299141No Hit
GACACTGGGGAACGCGAGCTGAGCAACGCCGAAGACAATGGCAGGCTCGG4400.10558420460299141No Hit
GACACTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC4390.10534424050162094No Hit
GACACTGGGGGTGTCAATGTGTCTGTCCTTCACTCCTCCATTGTCTGCCT4380.10510427640025052No Hit
GACACTGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGT4350.10438438409613922No Hit
GACACTGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCT4260.1022247071838053No Hit
GACACTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC4230.101504814879694No Hit
GACACTGGGGGGCACCCGCGCAGAACCAGCTCCCTGAGCTGCCCTAAGCA4200.10078492257558269No Hit
GACACTGGGAAATGGAAGAGATGTGTTTTCTCACATCTTTTAGATATAAT4200.10078492257558269No Hit
GACACTGGGGGCAGGCCGAGCGCCGGGAGCGAGGTGGCTCAGACATGGAC4200.10078492257558269No Hit
GACACTGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC4170.10006503027147139No Hit
GACACTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCG4170.10006503027147139No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGT254.4379405E-544.042
ACAACGT302.5238187E-644.029
AAACTTT207.850699E-444.042
GAAATTA600.044.042
TTAGGTT254.4379405E-544.016
TTTAGAT700.044.039
CTTCGAT254.4379405E-544.018
AACCGTG254.4379405E-544.025
AACCGTC254.4379405E-544.011
TTAGGCC302.5238187E-644.043
TCGCATC254.4379405E-544.030
CCTTACG207.850699E-444.022
ATCTCGT502.7284841E-1144.011
AATCCAA207.850699E-444.020
GGTACAC254.4379405E-544.029
CGAACGA351.4431134E-744.020
ACCGGAT207.850699E-444.031
ACACGAA302.5238187E-644.013
CTGTATT207.850699E-444.037
GATAATC207.850699E-444.043
Produced by FastQC (version 0.11.5)