##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1527540_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 416729 Sequences flagged as poor quality 0 Sequence length 50 %GC 60 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.106520064598335 31.0 31.0 33.0 28.0 34.0 2 30.13421672117851 31.0 30.0 33.0 26.0 34.0 3 31.663133595214155 31.0 31.0 33.0 30.0 34.0 4 35.24007448485707 35.0 35.0 37.0 33.0 37.0 5 35.957034427649624 37.0 35.0 37.0 35.0 37.0 6 36.09274372553866 37.0 35.0 37.0 35.0 37.0 7 36.43150344708432 37.0 37.0 37.0 35.0 37.0 8 36.58589874954707 37.0 37.0 37.0 35.0 37.0 9 38.58180496197769 39.0 39.0 39.0 38.0 39.0 10 37.61633819580591 39.0 37.0 39.0 35.0 39.0 11 37.254460332734226 39.0 37.0 39.0 34.0 39.0 12 36.9858517165832 39.0 37.0 39.0 34.0 39.0 13 36.946559994624806 39.0 37.0 39.0 33.0 39.0 14 37.806408961219404 40.0 38.0 40.0 33.0 40.0 15 37.83121165073705 40.0 38.0 40.0 33.0 40.0 16 37.941501551367914 40.0 38.0 40.0 34.0 40.0 17 37.862548562735014 40.0 37.0 40.0 34.0 40.0 18 37.692514799785954 40.0 37.0 40.0 33.0 40.0 19 37.61086221501263 39.0 37.0 40.0 33.0 40.0 20 37.405018609216064 39.0 36.0 40.0 33.0 40.0 21 37.31780365657298 39.0 36.0 40.0 33.0 40.0 22 37.092585349231754 38.0 36.0 40.0 33.0 40.0 23 36.915674215137415 38.0 36.0 40.0 32.0 40.0 24 36.7268080695128 38.0 35.0 40.0 32.0 40.0 25 36.497615956652886 38.0 35.0 40.0 32.0 40.0 26 35.94626003949809 38.0 35.0 39.0 31.0 40.0 27 35.734002193271884 38.0 34.0 39.0 30.0 40.0 28 35.47426744958954 37.0 34.0 39.0 30.0 40.0 29 35.14577339230051 37.0 34.0 38.0 30.0 40.0 30 34.84898339208454 36.0 33.0 38.0 29.0 40.0 31 34.69167732507217 36.0 33.0 38.0 29.0 39.0 32 34.490690592687336 36.0 33.0 38.0 29.0 39.0 33 33.99418327018278 36.0 33.0 38.0 27.0 39.0 34 33.39246368743236 35.0 32.0 38.0 26.0 39.0 35 33.29822498554216 35.0 31.0 38.0 26.0 39.0 36 32.94716710380127 34.0 31.0 37.0 26.0 38.0 37 32.62991536466145 34.0 31.0 37.0 25.0 38.0 38 32.18175360965999 34.0 30.0 36.0 24.0 38.0 39 31.281266722498316 33.0 29.0 36.0 23.0 38.0 40 30.824713422871937 33.0 29.0 36.0 21.0 38.0 41 30.492420733858214 32.0 28.0 35.0 21.0 38.0 42 30.25993391388648 32.0 28.0 35.0 21.0 37.0 43 29.64359331843956 31.0 27.0 34.0 20.0 37.0 44 29.215063026571226 31.0 26.0 34.0 20.0 36.0 45 28.6141305260733 30.0 26.0 34.0 19.0 36.0 46 28.590489262806283 30.0 26.0 33.0 20.0 36.0 47 28.00744848570654 30.0 24.0 33.0 18.0 36.0 48 27.428952628686748 29.0 24.0 33.0 17.0 35.0 49 26.662744853369936 29.0 23.0 32.0 15.0 34.0 50 30.341843260248268 32.0 28.0 34.0 22.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 8.0 14 25.0 15 40.0 16 73.0 17 149.0 18 209.0 19 393.0 20 526.0 21 771.0 22 1104.0 23 1558.0 24 2199.0 25 3121.0 26 4191.0 27 5706.0 28 7552.0 29 10347.0 30 13950.0 31 19630.0 32 28528.0 33 40318.0 34 60431.0 35 108149.0 36 95500.0 37 11971.0 38 276.0 39 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 100.0 0.0 7 99.53974885357151 0.3052343369431933 0.09262614312898791 0.06239066635631309 8 99.42768561823151 0.36282572127209767 0.1446983531263723 0.06479030737001745 9 98.59045086855006 0.6913365760482232 0.26851982943351677 0.44969272596819515 10 69.0443909591125 18.770231973296795 4.537001264610814 7.648375802979874 11 36.25425636324806 27.00484007592464 15.747164224232055 20.993739336595247 12 29.83425679518344 16.409945072217198 28.13291131646706 25.622886816132308 13 22.99288026511234 15.820353275150037 30.600942099061978 30.585824360675645 14 24.920991819623783 16.128947109512417 30.553429206990636 28.39663186387316 15 25.34332863803575 16.164221832413872 27.64938365220563 30.84306587734475 16 29.624768134687052 17.116879314854497 24.021366403586022 29.23698614687243 17 32.46762284362259 17.35660345212356 20.918390608764927 29.257383095488915 18 31.620789529886327 18.75055491698442 21.270177981373987 28.358477571755262 19 31.636147232374036 16.628312404464292 20.751135630109736 30.984404733051935 20 31.004081789364314 19.93549764955163 21.65196086665435 27.408459694429716 21 36.611082981985895 15.833311336624039 22.971283495989 24.584322185401064 22 34.52123562315077 16.525127840875005 20.308881791284026 28.644754744690193 23 34.000513523176934 19.25351967345685 20.22489435580437 26.521072447561846 24 30.751159626519875 18.300142298712114 21.867448629685 29.081249445083017 25 30.145730198762266 18.130487679043213 22.098294095203357 29.625488026991164 26 32.39971300293476 17.71367003496277 19.35238488322147 30.534232078881 27 30.75067969831713 15.838590546854189 22.85058155299967 30.56014820182901 28 31.484969848510662 19.583710276942572 19.508121585010883 29.423198289535886 29 33.14816103510915 19.60794665118098 19.701532650715453 27.542359662994414 30 32.482260653806186 17.488343743775932 19.901422747157024 30.127972855260854 31 33.807582385675104 19.48388521077247 19.07066702821258 27.63786537533985 32 35.140822932889236 19.371102083128363 19.30415209884601 26.183922885136386 33 38.12189696421415 17.181909586325887 18.40428671870688 26.291906730753077 34 32.990264656407405 21.479426677769005 20.849280947570243 24.681027718253347 35 31.890029251623957 23.103263751742737 22.33681841196557 22.66988858466773 36 35.17489783528384 19.674176743159226 18.61617502021698 26.534750401339956 37 32.78533531383705 23.093905151789293 19.0550693616235 25.065690172750156 38 36.26745438882343 20.656589774169785 19.19688814553343 23.879067691473356 39 33.76582863203665 20.061718766872477 19.94245660849137 26.22999599259951 40 31.22484876262511 21.462629190673074 20.68226593301642 26.63025611368539 41 31.41777990012694 19.764403245274504 20.838962491211316 27.97885436338724 42 29.45775312013323 18.721039332515858 22.918491393687503 28.90271615366341 43 29.668201637035104 16.678464901650713 25.3145329458713 28.33880051544289 44 27.68273866229612 18.05729862812523 24.96850471169513 29.29145799788352 45 28.831206851455022 17.325408118945408 24.697105313045167 29.146279716554403 46 30.332662233729835 17.727827916943625 24.824046322670128 27.115463526656413 47 29.411919976771472 19.834712726976043 23.63550412858236 27.117863167670116 48 31.69253879619609 18.12688821752266 23.255161027910223 26.925411958371026 49 29.772346057029868 19.482205462062876 23.404418698962633 27.34102978194462 50 28.241375090286493 20.96206407521435 22.616136625960756 28.1804242085384 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 4.0 6 8.0 7 7.0 8 6.0 9 6.0 10 6.0 11 18.5 12 31.0 13 83.0 14 135.0 15 159.5 16 184.0 17 178.0 18 172.0 19 302.5 20 433.0 21 462.5 22 492.0 23 577.5 24 663.0 25 770.5 26 878.0 27 955.5 28 1033.0 29 1275.0 30 1517.0 31 1484.0 32 1451.0 33 1394.5 34 1338.0 35 1703.5 36 2069.0 37 2229.0 38 2389.0 39 3089.0 40 3789.0 41 3834.5 42 3880.0 43 4238.5 44 4597.0 45 5595.0 46 6593.0 47 6999.5 48 7406.0 49 9852.0 50 12298.0 51 12359.0 52 12420.0 53 15163.5 54 17907.0 55 22208.0 56 26509.0 57 35425.5 58 44342.0 59 44375.5 60 44409.0 61 44033.0 62 43657.0 63 41699.0 64 39741.0 65 39443.5 66 39146.0 67 33811.5 68 28477.0 69 23942.5 70 19408.0 71 18765.0 72 18122.0 73 14431.0 74 10740.0 75 12011.0 76 13282.0 77 9096.0 78 4910.0 79 3222.0 80 1534.0 81 1006.5 82 479.0 83 372.0 84 265.0 85 139.0 86 13.0 87 6.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 416729.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 12.79392602866611 #Duplication Level Percentage of deduplicated Percentage of total 1 73.61955135419012 9.418830942890944 2 8.85100157551204 2.2647811887341653 3 2.997224097831795 1.1503879019698653 4 1.3973291319678895 0.7150930220838962 5 0.7539950483907271 0.48232784375457427 6 0.4970365368744842 0.38154292117899163 7 0.4107584965113662 0.3678649674008769 8 0.3601170380373621 0.3685848597049881 9 0.251331682796909 0.2893967062527446 >10 7.299872458549029 26.404929822498552 >50 2.297621727061295 20.144746345946647 >100 1.1947632980718734 29.10548581932143 >500 0.05439267762022658 4.571796059309528 >1k 0.015004876584890088 4.334231598952797 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GACACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT 4825 1.1578267891123488 No Hit GACACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC 3389 0.8132383395444042 No Hit GACACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC 2417 0.5799932330123414 No Hit GACACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG 1844 0.44249380292708207 No Hit GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC 1601 0.3841825262940664 No Hit GACACTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG 1476 0.354187013622762 No Hit GACACTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG 1377 0.33043056758708894 No Hit GACACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC 1133 0.27187932685270283 No Hit GACACTGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGT 939 0.22532629118683845 No Hit GACACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGACACTGGGA 916 0.21980711685531845 No Hit GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC 896 0.21500783482790975 No Hit GACACTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCT 886 0.21260819381420537 No Hit GACACTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA 855 0.20516930667172192 No Hit GACACTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG 846 0.203009629759388 No Hit GACACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG 799 0.19173131699497756 No Hit GACACTGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAA 704 0.16893472736478624 No Hit GACACTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG 689 0.1653352658442297 No Hit GACACTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGT 688 0.16509530174285927 No Hit GACACTGGGTGCTGACACTGGGGCTGACACTGGGGGGGTTGGGGATTTAG 661 0.1586162710058575 No Hit GACACTGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTT 653 0.15669655819489403 No Hit GACACTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT 635 0.15237720437022623 No Hit GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG 602 0.14445838902500185 No Hit GACACTGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTA 602 0.14445838902500185 No Hit GACACTGGGGAAGCTCGAGCTGCCGCGTCCGCCCTGCGCCTGCTCTCCTG 598 0.1434985326195201 No Hit GACACTGGGGAGACTGAGAAGGAGGACAGGAGCGGGCTGGCGATCTGGGA 597 0.1432585685181497 No Hit GACACTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA 573 0.13749943008525925 No Hit GACACTGGGGGTTGGGGTCGGACCGCTGCAGGGGATGGTGCGATTTGGGC 571 0.13701950188251835 No Hit GACACTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTC 566 0.1358196813756662 No Hit GACACTGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA 554 0.132940112159221 No Hit GACACTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT 552 0.1324601839564801 No Hit GACACTGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC 546 0.13102039934825752 No Hit GACACTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGT 540 0.1295806147400349 No Hit GACACTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGA 530 0.12718097372633053 No Hit GACACTGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAA 526 0.12622111732084879 No Hit GACACTGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTC 517 0.1240614404085149 No Hit GACACTGGGGGCCACCAGCGGCAAACTAGGACTGTGCGTTTGCTTTCTCA 507 0.12166179939481053 No Hit GACACTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCAT 504 0.12094190709069923 No Hit GACACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA 499 0.11974208658384707 No Hit GACACTGGGCAGGGGTTCATTGCCTGGGACAAGGGCTCCAGAACCCACCT 484 0.11614262506329054 No Hit GACACTGGGGGCAGCCGCCGGAGCCGAGATGCCTAAAGGAGGTAGAAAGG 482 0.11566269686054965 No Hit GACACTGGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTG 478 0.11470284045506793 No Hit GACACTGGGGTTCTCCCCAGGTTGGTTCGGCCCCCGTCTACTCTGGGGTG 473 0.11350301994821575 No Hit GACACTGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCC 457 0.10966359432628878 No Hit GACACTGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT 453 0.10870373792080705 No Hit GACACTGGGGGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCGTTC 452 0.10846377381943662 No Hit GACACTGGGCTGAGCCAAAACCACTAGTCCAAGAAAAAGAAGAAATTAAA 442 0.10606413280573226 No Hit GACACTGGGGGCAGGAAGCGGAAGAACCGTAGGGTGGGCGGCTCTTTGTC 440 0.10558420460299141 No Hit GACACTGGGGAACGCGAGCTGAGCAACGCCGAAGACAATGGCAGGCTCGG 440 0.10558420460299141 No Hit GACACTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC 439 0.10534424050162094 No Hit GACACTGGGGGTGTCAATGTGTCTGTCCTTCACTCCTCCATTGTCTGCCT 438 0.10510427640025052 No Hit GACACTGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGT 435 0.10438438409613922 No Hit GACACTGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCT 426 0.1022247071838053 No Hit GACACTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCC 423 0.101504814879694 No Hit GACACTGGGGGGCACCCGCGCAGAACCAGCTCCCTGAGCTGCCCTAAGCA 420 0.10078492257558269 No Hit GACACTGGGAAATGGAAGAGATGTGTTTTCTCACATCTTTTAGATATAAT 420 0.10078492257558269 No Hit GACACTGGGGGCAGGCCGAGCGCCGGGAGCGAGGTGGCTCAGACATGGAC 420 0.10078492257558269 No Hit GACACTGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC 417 0.10006503027147139 No Hit GACACTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCG 417 0.10006503027147139 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 4.7992820274086995E-4 0.0 0.0 0.0 0.0 8 7.198923041113049E-4 0.0 0.0 0.0 0.0 9 9.598564054817399E-4 0.0 0.0 0.0 0.0 10 0.007438887142483484 0.0 0.0 0.0 0.0 11 0.017757343501412188 0.0 0.0 0.0 0.0 12 0.028555728063081764 0.0 0.0 0.0 0.0 13 0.034554830597342635 0.0 0.0 0.0 0.0 14 0.04463332285490091 0.0 0.0 0.0 0.0 15 0.051352317693273086 0.0 0.0 0.0 0.0 16 0.062390666356313094 0.0 0.0 0.0 0.0 17 0.07006951760016701 0.0 0.0 0.0 0.0 18 0.07510876372894615 0.0 0.0 0.0 0.0 19 0.08374747137828181 0.0 0.0 0.0 0.0 20 0.0926261431289879 0.0 0.0 0.0 0.0 21 0.09910517386598965 0.0 0.0 0.0 0.0 22 0.10486431229888009 0.0 0.0 0.0 0.0 23 0.11422291225232706 0.0 0.0 0.0 0.0 24 0.1255012250167375 0.0 0.0 0.0 0.0 25 0.13629960957840706 0.0 0.0 0.0 0.0 26 0.14493831722774272 0.0 0.0 0.0 0.0 27 0.1557367017894123 0.0 0.0 0.0 0.0 28 0.19869027593472016 0.0 0.0 0.0 0.0 29 0.2092486963950193 0.0 0.0 0.0 0.0 30 0.2277259322005428 0.0 0.0 0.0 0.0 31 0.2394841731676941 0.0 0.0 0.0 0.0 32 0.2550818397567724 0.0 0.0 0.0 0.0 33 0.2618008345951446 0.0 0.0 0.0 0.0 34 0.2749988601705185 0.0 0.0 0.0 0.0 35 0.3129131881870472 0.0 0.0 0.0 0.0 36 0.3251513573569394 0.0 0.0 0.0 0.0 37 0.337629490628202 0.0 0.0 0.0 0.0 38 0.34602823417616724 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACGGT 25 4.4379405E-5 44.0 42 ACAACGT 30 2.5238187E-6 44.0 29 AAACTTT 20 7.850699E-4 44.0 42 GAAATTA 60 0.0 44.0 42 TTAGGTT 25 4.4379405E-5 44.0 16 TTTAGAT 70 0.0 44.0 39 CTTCGAT 25 4.4379405E-5 44.0 18 AACCGTG 25 4.4379405E-5 44.0 25 AACCGTC 25 4.4379405E-5 44.0 11 TTAGGCC 30 2.5238187E-6 44.0 43 TCGCATC 25 4.4379405E-5 44.0 30 CCTTACG 20 7.850699E-4 44.0 22 ATCTCGT 50 2.7284841E-11 44.0 11 AATCCAA 20 7.850699E-4 44.0 20 GGTACAC 25 4.4379405E-5 44.0 29 CGAACGA 35 1.4431134E-7 44.0 20 ACCGGAT 20 7.850699E-4 44.0 31 ACACGAA 30 2.5238187E-6 44.0 13 CTGTATT 20 7.850699E-4 44.0 37 GATAATC 20 7.850699E-4 44.0 43 >>END_MODULE