FastQCFastQC Report
Fri 17 Jun 2016
SRR1527539_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527539_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382479
Sequences flagged as poor quality0
Sequence length52
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG42341.1069888804352657No Hit
GACACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT31520.824097532152092No Hit
GACACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC24060.6290541441490906No Hit
GACACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG18480.4831637815409473No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA16790.4389783491381226No Hit
GACACTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG13960.36498735878309657No Hit
GACACTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT11750.30720640871786425No Hit
GACACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC11090.2899505593771161No Hit
GACACTGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCG9230.24132043850773505No Hit
GACACTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG8830.23086234799819078No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC8540.22328023237877115No Hit
GACACTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG8270.2162210212848287No Hit
GACACTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA7960.2081160011399319No Hit
GACACTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC6910.18066351355237803No Hit
GACACTGGGTGCTGACACTGGGGCTGACACTGGGGGGGTTGGGGATTTAGCT6900.18040206128963943No Hit
GACACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC6880.17987915676416222No Hit
GACACTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC6360.16628363910175462No Hit
GACACTGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAAT6290.16445347326258436No Hit
GACACTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG6280.16419202099984576No Hit
GACACTGGGGAAGCTCGAGCTGCCGCGTCCGCCCTGCGCCTGCTCTCCTGCT6100.1594858802705508No Hit
GACACTGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA6000.15687135764316473No Hit
GACACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGACACTGGGAAG5750.15033505107469952No Hit
GACACTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA5690.14876633749826787No Hit
GACACTGGGGAGACTGAGAAGGAGGACAGGAGCGGGCTGGCGATCTGGGAAG5630.14719762392183625No Hit
GACACTGGGGGTTGGGGTCGGACCGCTGCAGGGGATGGTGCGATTTGGGCTG5600.1464132671336204No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT5470.1430143877180185No Hit
GACACTGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC5460.1427529354552799No Hit
GACACTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG5320.13909260377693938No Hit
GACACTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA5300.13856969925146218No Hit
GACACTGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT5290.13830824698872354No Hit
GACACTGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA5270.13778534246324636No Hit
GACACTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA5220.1364780811495533No Hit
GACACTGGGGGCCACCAGCGGCAAACTAGGACTGTGCGTTTGCTTTCTCAGA5210.1362166288868147No Hit
GACACTGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATG4970.1299417745810881No Hit
GACACTGGGGTTCTCCCCAGGTTGGTTCGGCCCCCGTCTACTCTGGGGTGGT4830.1262814429027476No Hit
GACACTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG4780.12497418158905456No Hit
GACACTGGGCTGAGCCAAAACCACTAGTCCAAGAAAAAGAAGAAATTAAAGA4760.12445127706357734No Hit
GACACTGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCAG4640.12131384991071405No Hit
GACACTGGGGGCAGCCGCCGGAGCCGAGATGCCTAAAGGAGGTAGAAAGGGA4610.12052949312249824No Hit
GACACTGGGCAGGGGTTCATTGCCTGGGACAAGGGCTCCAGAACCCACCTCT4590.12000658859702101No Hit
GACACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC4540.11869932728332798No Hit
GACACTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT4530.11843787502058938No Hit
GACACTGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA4430.11582335239320328No Hit
GACACTGGGGAACGCGAGCTGAGCAACGCCGAAGACAATGGCAGGCTCGGCG4420.11556190013046468No Hit
GACACTGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT4400.11503899560498745No Hit
GACACTGGGTTCTTTCCCTCTCGTCTCTATGGTTGCGCCGGCGTTGTCAGGA4390.11477754334224886No Hit
GACACTGGGGGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCGTTCCG4380.11451609107951025No Hit
GACACTGGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGT4350.11373173429129442No Hit
GACACTGGGGGGCACCCGCGCAGAACCAGCTCCCTGAGCTGCCCTAAGCAGC4220.11033285487569253No Hit
GACACTGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG4200.10980995035021532No Hit
GACACTGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG4190.10954849808747669No Hit
GACACTGGGAGTTCGAGGCGCCGCCGCCGCCGCTGCAGCCGCCGGAGCCGAG4170.10902559356199948No Hit
GACACTGGGGACTGGCTGCTCCGAAAAGCCATCTTTGCATTGTTCCTGGGTC4100.10719542772282924No Hit
GACACTGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT4090.10693397546009062No Hit
GACACTGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC4030.10536526188365898No Hit
GACACTGGGGGCAGGAAGCGGAAGAACCGTAGGGTGGGCGGCTCTTTGTCGA4000.10458090509544314No Hit
GACACTGGGGGCAGGCCGAGCGCCGGGAGCGAGGTGGCTCAGACATGGACCG3940.1030121915190115No Hit
GACACTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAG3910.10222783473079569No Hit
GACACTGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG3890.10170493020531847No Hit
GACACTGGGGCGTCAGCGTCTTCCTTTCGCCGCCGGACGCCGCCGAGGTCGC3860.10092057341710264No Hit
GACACTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGC3850.10065912115436403No Hit
GACACTGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT3850.10065912115436403No Hit
GACACTGGGGCCCGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGAT3840.10039766889162544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAA351.0171789E-746.00000413
ACTCTTT351.0171789E-746.00000411
CACGAAT351.0171789E-746.00000414
CAACACG351.0171789E-746.00000411
TTGACGA351.0171789E-746.00000428
TGGCTAT351.0171789E-746.00000433
ATTTCGG351.0171789E-746.00000443
ACGAATA351.0171789E-746.00000415
AACACGA351.0171789E-746.00000412
TTGATCT453.092282E-1046.044
CATGGTA253.4128512E-546.036
GAAATTA405.5952114E-946.042
TAATATC206.3061476E-446.021
TATCAAG206.3061476E-446.012
TTAGGGG253.4128512E-546.034
GAAATAC253.4128512E-546.010
TAATACA253.4128512E-546.027
ACTATCA206.3061476E-446.022
AACCGAT206.3061476E-446.045
ATCTCGT501.6370905E-1146.011
Produced by FastQC (version 0.11.5)