FastQCFastQC Report
Fri 17 Jun 2016
SRR1527538_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527538_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405422
Sequences flagged as poor quality0
Sequence length52
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACACTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG49831.2290896892620529No Hit
GACACTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT34520.8514584802995397No Hit
GACACTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC24570.6060351929594349No Hit
GACACTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG18680.46075447311690043No Hit
GACACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA15310.3776312089625132No Hit
GACACTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG14460.356665400496273No Hit
GACACTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT13730.3386594708723256No Hit
GACACTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC12970.3199135715378051No Hit
GACACTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC9130.22519744858443794No Hit
GACACTGGGGAGAGTGTGGCCAGGCGGCTCGGACCGAGCAGGGCTTTCCTTG9070.22371750916329158No Hit
GACACTGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCG8750.21582449891717767No Hit
GACACTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA8690.21434455949603132No Hit
GACACTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG8420.20768483210087266No Hit
GACACTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC7970.19658528644227496No Hit
GACACTGGGTGCTGACACTGGGGCTGACACTGGGGGGGTTGGGGATTTAGCT6600.1627933363260997No Hit
GACACTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTC6530.1610667403347623No Hit
GACACTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC6460.15934014434342486No Hit
GACACTGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA6440.15884683120304274No Hit
GACACTGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA6400.1578602049222785No Hit
GACACTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG6300.15539363922036792No Hit
GACACTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGACACTGGGAAG6270.15465366950979473No Hit
GACACTGGGGAAGCTCGAGCTGCCGCGTCCGCCCTGCGCCTGCTCTCCTGCT6170.1521871038078841No Hit
GACACTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA6130.1512004775271199No Hit
GACACTGGGGCTCTATCTGCGGCGTGTGGCGGCGAGATGTCTCACAGGAAAT6100.1504605078165467No Hit
GACACTGGGGAGACTGAGAAGGAGGACAGGAGCGGGCTGGCGATCTGGGAAG6050.1492272249655914No Hit
GACACTGGGGGTTGGGGTCGGACCGCTGCAGGGGATGGTGCGATTTGGGCTG5950.14676065926368081No Hit
GACACTGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC5850.1442940935617702No Hit
GACACTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT5630.13886764901756687No Hit
GACACTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT5480.13516780046470098No Hit
GACACTGGGGCTCTCGAACCAGGCTGCTTCAGCCGTGAACAACGCCGAAATG5420.13368786104355462No Hit
GACACTGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT5220.12875472963973342No Hit
GACACTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA5090.12554819422724964No Hit
GACACTGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCAG5010.12357494166572115No Hit
GACACTGGGGGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCGTTCCG4960.12234165881476586No Hit
GACACTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC4900.12086171939361949No Hit
GACACTGGGCAGGGGTTCATTGCCTGGGACAAGGGCTCCAGAACCCACCTCT4870.12012174968304631No Hit
GACACTGGGGGCAGCCGCCGGAGCCGAGATGCCTAAAGGAGGTAGAAAGGGA4860.11987509311285524No Hit
GACACTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCA4860.11987509311285524No Hit
GACACTGGGTTCTTTCCCTCTCGTCTCTATGGTTGCGCCGGCGTTGTCAGGA4840.11938177997247314No Hit
GACACTGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGC4800.11839515369170889No Hit
GACACTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGG4780.11790184055132677No Hit
GACACTGGGGTTCTCCCCAGGTTGGTTCGGCCCCCGTCTACTCTGGGGTGGT4780.11790184055132677No Hit
GACACTGGGGGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGT4770.1176551839811357No Hit
GACACTGGGGGGCACCCGCGCAGAACCAGCTCCCTGAGCTGCCCTAAGCAGC4740.11691521427056252No Hit
GACACTGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG4740.11691521427056252No Hit
GACACTGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT4650.11469530513884298No Hit
GACACTGGGAAATGGAAGAGATGTGTTTTCTCACATCTTTTAGATATAATTC4650.11469530513884298No Hit
GACACTGGGCTGAGCCAAAACCACTAGTCCAAGAAAAAGAAGAAATTAAAGA4590.11321536571769662No Hit
GACACTGGGGGCCACCAGCGGCAAACTAGGACTGTGCGTTTGCTTTCTCAGA4590.11321536571769662No Hit
GACACTGGGGAACGCGAGCTGAGCAACGCCGAAGACAATGGCAGGCTCGGCG4570.1127220525773145No Hit
GACACTGGGGGCAGGCCGAGCGCCGGGAGCGAGGTGGCTCAGACATGGACCG4570.1127220525773145No Hit
GACACTGGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGA4550.11222873943693239No Hit
GACACTGGGGTATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG4460.11000883030521284No Hit
GACACTGGGGCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCT4400.10852889088406649No Hit
GACACTGGGGATCTTGACTCCCTGACCTTGGACTGGTCCTCGTCCGTGGTCG4340.10704895146292012No Hit
GACACTGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC4260.10507569890139165No Hit
GACACTGGGGCCCGCCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGAT4230.10433572919081846No Hit
GACACTGGGGCGAACGGCGAGCAGCGGCGGCGGCGCGGGAAGTGCAGCGGAG4190.10334910291005421No Hit
GACACTGGGGGCACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTA4180.10310244633986315No Hit
GACACTGGGAGTTCGAGGCGCCGCCGCCGCCGCTGCAGCCGCCGGAGCCGAG4170.1028557897696721No Hit
GACACTGGGGGGCCCTTTCCCAGGCTCCTTTGCGGGTAAACAGACATGGCCG4110.10137585034852574No Hit
GACACTGGGGCTCTCTCCTTCCTGCGGCGCCTTAGGGACCATGGCCGATCCT4080.10063588063795256No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATAC351.01737896E-746.00000410
TTTAATC351.01737896E-746.00000441
GATTTAA351.01737896E-746.00000421
GCGTTAG351.01737896E-746.00000433
ATCGTTT351.01737896E-746.00000420
ATGTAGG351.01737896E-746.00000419
CGTTAGG351.01737896E-746.00000434
AATGCGT351.01737896E-746.00000430
ACTAGAC351.01737896E-746.00000436
AAACCCC351.01737896E-746.00000423
AGATTTA351.01737896E-746.00000420
TGGGCGA1450.046.0000046
CGCAAAG351.01737896E-746.00000417
TTATTAG351.01737896E-746.00000438
AGGGATA351.01737896E-746.00000438
ATACCGC351.01737896E-746.00000413
TACCGCA351.01737896E-746.00000414
TCACGCG253.4132157E-546.041
ATTTATA206.306597E-446.040
TTAGGCC206.306597E-446.043
Produced by FastQC (version 0.11.5)