FastQCFastQC Report
Fri 17 Jun 2016
SRR1527537_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527537_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences665873
Sequences flagged as poor quality0
Sequence length50
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC54340.8160715331602273No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC44950.6750536513719583No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG40800.6127294544154815No Hit
TGGATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA19330.2902955969081191No Hit
TGGATGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT18020.27062217570017105No Hit
TGGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT17660.2652157393376815No Hit
TGGATGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA16170.24283909994848868No Hit
TGGATGGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACT15720.23608105449537675No Hit
TGGATGGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTA15660.23517998176829516No Hit
TGGATGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA15500.23277712116274424No Hit
TGGATGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG15400.23127533328427494No Hit
TGGATGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG14340.21535638177250016No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC13910.2088986938950821No Hit
TGGATGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAA13420.20153993329058242No Hit
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13160.19763528480656223No Hit
TGGATGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCA11090.16654827572224734No Hit
TGGATGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC10420.1564862969365029No Hit
TGGATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTA10300.15468415148233972No Hit
TGGATGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG9690.1455232454236769No Hit
TGGATGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA9430.14161859693965667No Hit
TGGATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC9190.1380143060313303No Hit
TGGATGGGGGAAGAGTCGGCCGCTGCAGGCGTGCGCGCACTGTTTGCTGC8870.1332085848202285No Hit
TGGATGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA8850.1329082272445346No Hit
TGGATGGGGTCTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTAT8780.1318569757296061No Hit
TGGATGGGGGGCTGATGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCT8780.1318569757296061No Hit
TGGATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC8430.1266007181549635No Hit
TGGATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG8380.12584982421572882No Hit
TGGATGGGGGGGCTGGTGAGATGGCTTAGTGGGTAAGAGCACCCGACTGC7600.11413587876366814No Hit
TGGATGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTCC7440.11173301815811723No Hit
TGGATGGGGATCTGAACAAGACAATTTGGCTCTGCCTAGTGTTCTGTGCC7430.11158283937027032No Hit
TGGATGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA7290.10948033634041326No Hit
TGGATGGGGCTGTAGAACGCAAGAAGACAGCTGTGGTTCTAAGTGCTAAA7130.10707747573486236No Hit
TGGATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC6910.10377354240222986No Hit
TGGATGGGGGGCACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTC6870.10317282725084212No Hit
TGGATGGGGACCTTTGTTCCTGTTGCATTGTGGGACATTCCTCGGAGGAA6830.10257211209945441No Hit
TGGATGGGTGCCCAGCGTGGTGGTGCACGCCTTTAATCCCAGCACTCAGG6790.10197139694806667No Hit
TGGATGGGGATTTTATCACAGGTGAGACCAAGGACCAGGTTGCTAACTCC6680.10031943028175042No Hit
TGGATGGGGATAAGAGTCGGCCGCTGCAGGCGTACGCGCACTGTTTGCTG6670.1001692514939035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA302.5261052E-644.00000417
TAGGTGA302.5261052E-644.00000421
AGGCGTA600.044.00000428
CGATTCA302.5261052E-644.00000442
CCGTACC302.5261052E-644.00000412
TGCGCAA302.5261052E-644.00000414
GTGTACG302.5261052E-644.00000432
CCGAAAT302.5261052E-644.00000444
CACGTAA600.044.00000428
CGCGTAT302.5261052E-644.00000415
CCTATAC302.5261052E-644.00000421
CGATCTC650.044.00000421
GCGTATA302.5261052E-644.00000416
GTTATCC302.5261052E-644.00000433
GCATAAC254.440816E-544.09
TCACGCC502.7284841E-1144.018
TTTAGCG207.8540936E-444.014
AAGTAGT207.8540936E-444.017
ACCGGTA207.8540936E-444.019
CAAACGC254.440816E-544.032
Produced by FastQC (version 0.11.5)