##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527537_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	665873
Sequences flagged as poor quality	0
Sequence length	50
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46500158438621	31.0	31.0	33.0	30.0	34.0
2	31.73542101872279	31.0	31.0	34.0	30.0	34.0
3	32.726838300997336	34.0	31.0	34.0	31.0	34.0
4	33.5249604654341	35.0	33.0	35.0	30.0	37.0
5	35.08892236207205	35.0	35.0	37.0	33.0	37.0
6	35.855485956030655	37.0	35.0	37.0	35.0	37.0
7	36.492413718531914	37.0	37.0	37.0	35.0	37.0
8	36.6449322918935	37.0	37.0	37.0	35.0	37.0
9	38.42765362163656	39.0	39.0	39.0	37.0	39.0
10	37.128012398760724	39.0	37.0	39.0	34.0	39.0
11	36.25996999427819	37.0	35.0	39.0	33.0	39.0
12	36.29417020963457	38.0	35.0	39.0	33.0	39.0
13	36.3074700430863	38.0	35.0	39.0	33.0	39.0
14	36.99508314648589	39.0	35.0	40.0	33.0	40.0
15	37.13840777445549	39.0	35.0	40.0	33.0	40.0
16	37.32075936402287	39.0	35.0	40.0	33.0	40.0
17	37.260817002641645	39.0	35.0	40.0	33.0	40.0
18	37.11747134964175	39.0	35.0	40.0	33.0	40.0
19	37.04683926214158	39.0	35.0	40.0	33.0	40.0
20	36.90241682723282	38.0	35.0	40.0	33.0	40.0
21	36.823627027976805	38.0	35.0	40.0	33.0	40.0
22	36.65533968189129	38.0	35.0	40.0	32.0	40.0
23	36.46856833059758	38.0	35.0	40.0	32.0	40.0
24	36.34703314295669	38.0	35.0	40.0	32.0	40.0
25	36.12618171933687	38.0	35.0	40.0	32.0	40.0
26	35.58067679572531	37.0	34.0	39.0	30.0	40.0
27	35.45780201329683	37.0	34.0	39.0	30.0	40.0
28	35.316731568932816	36.0	34.0	39.0	30.0	40.0
29	35.03297776002331	36.0	34.0	38.0	30.0	40.0
30	34.745039970084385	36.0	33.0	38.0	29.0	40.0
31	34.5525468069737	36.0	33.0	38.0	29.0	40.0
32	34.457325946539356	35.0	33.0	38.0	29.0	40.0
33	33.93537055865007	35.0	33.0	38.0	27.0	39.0
34	33.427330737242684	35.0	32.0	38.0	26.0	39.0
35	33.358877743954174	35.0	32.0	38.0	26.0	39.0
36	32.99176419527448	34.0	31.0	37.0	25.0	38.0
37	32.79645517989166	34.0	31.0	37.0	25.0	38.0
38	32.295922796088746	34.0	31.0	37.0	24.0	38.0
39	31.47022179905177	33.0	30.0	36.0	23.0	38.0
40	31.202962126411492	33.0	29.0	36.0	22.0	38.0
41	30.923450868258662	33.0	29.0	35.0	21.0	38.0
42	30.556917009700047	32.0	29.0	35.0	21.0	38.0
43	30.08857544907212	32.0	27.0	35.0	21.0	37.0
44	29.511507449618772	31.0	27.0	34.0	20.0	37.0
45	29.06507847592559	31.0	26.0	34.0	20.0	36.0
46	28.996289082152302	31.0	26.0	34.0	20.0	36.0
47	28.531907736159898	31.0	25.0	34.0	19.0	36.0
48	28.061753517562657	30.0	24.0	33.0	18.0	35.0
49	27.768742688170267	30.0	24.0	33.0	18.0	35.0
50	30.971620714460567	33.0	29.0	35.0	23.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	5.0
14	18.0
15	27.0
16	76.0
17	169.0
18	322.0
19	527.0
20	859.0
21	1330.0
22	1869.0
23	2725.0
24	3774.0
25	4904.0
26	6864.0
27	9555.0
28	13185.0
29	17581.0
30	24709.0
31	36700.0
32	59092.0
33	74901.0
34	74875.0
35	134946.0
36	163232.0
37	32982.0
38	643.0
39	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	98.81854347600819	0.8827509149642649	0.2018402908662763	0.09686531816127099
8	98.9693229790065	0.8051084816474011	0.11143266058242338	0.11413587876366814
9	93.82374717100708	3.2141264175000335	1.9201859814108695	1.0419404300820125
10	55.22043993374112	31.178167608537965	5.7860883381665875	7.815304119554329
11	36.251056883219476	24.085373637315225	21.076992159165485	18.586577320299817
12	31.98808181139647	18.123275759792033	25.28785519160561	24.600787237205893
13	26.40323304894477	18.765740614201206	27.784727718348694	27.046298618505332
14	24.085673994890918	19.19179783532295	30.790405978317185	25.93212219146894
15	27.262856430580605	19.44334730496656	28.152966106149368	25.140830158303462
16	32.37283986586031	20.560527307759887	23.95486827067624	23.111764555703566
17	30.757216466203012	21.582493959058258	23.279213904152893	24.381075670585833
18	31.447738532723207	20.893023144052993	22.67098981337282	24.988248509850976
19	28.89469913932537	22.895056564840445	22.39691352555217	25.813330770282022
20	30.16611275723749	23.321414143537883	22.355614358894265	24.156858740330364
21	29.890684860326218	21.336200746989288	24.534107855401857	24.239006537282634
22	29.722935154301194	20.99694686524307	25.576649000635253	23.703468979820478
23	29.881824311843246	20.954596447070237	24.874262809875155	24.289316431211358
24	28.882084121146224	21.26922100760956	23.789221067681073	26.059473803563144
25	29.24942143621982	22.17705178014426	21.437271071210276	27.136255712425644
26	28.330477433384445	21.938117328679795	22.97344989209654	26.757955345839225
27	26.308470233813352	21.124448656125118	23.282067301121987	29.285013808939542
28	27.299500054815258	23.42233428897102	21.75565010144577	27.522515554767953
29	28.65561450907305	23.092541670859156	22.244782413463227	26.007061406604564
30	29.475140154353756	21.319981437901824	23.924532155531157	25.28034625221326
31	30.17827723905309	21.981969534731096	22.029726389266422	25.81002683694939
32	33.332332141414355	20.44834375323823	22.27391709830553	23.945407007041883
33	33.82702106858215	21.778327098410657	21.98767632866928	22.406975504337915
34	28.229106751587764	22.99852974966698	25.614944591536226	23.15741890720903
35	29.232150875617425	26.19448453383753	23.3989063980669	21.174458192478145
36	28.445664563663044	27.215399933620976	21.954787174130804	22.38414832858518
37	30.097330872403592	25.366699055225244	21.80280624082971	22.73316383154145
38	31.48393162059432	24.086725246405845	20.9442341107088	23.485109022291038
39	31.557969763002852	21.875042237784083	20.22953325934525	26.33745473986781
40	28.159874330390327	23.11687063449036	21.96665129837071	26.756603736748595
41	27.20293509422968	24.294572688786	21.71900647721112	26.783485739773198
42	26.444532215602674	21.752946883264528	22.896858710294605	28.905662190838193
43	26.308770591389045	20.672260325918003	25.241449946160905	27.777519136532042
44	25.69979560666974	20.096625032100714	24.831161497763087	29.372417863466456
45	27.705583497153363	20.974269868278185	24.444150761481545	26.87599587308691
46	26.933213991256586	23.26539745567098	22.466746661900995	27.33464189117144
47	26.08950956113253	22.207087537713647	25.956901691463685	25.746501209690138
48	26.607025664053058	23.252932616279683	25.385471403706113	24.754570315961153
49	29.002978045363005	22.449025564935056	22.579230573998345	25.96876581570359
50	25.75355961271894	22.531623898250867	24.626017273564177	27.088799215466015
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	3.5
8	6.0
9	27.0
10	48.0
11	189.0
12	330.0
13	379.0
14	428.0
15	493.5
16	559.0
17	631.5
18	704.0
19	777.0
20	850.0
21	931.0
22	1012.0
23	1388.0
24	1764.0
25	2268.0
26	2772.0
27	2850.5
28	2929.0
29	3367.5
30	3806.0
31	4529.0
32	5252.0
33	5620.5
34	5989.0
35	8601.5
36	11214.0
37	11582.0
38	11950.0
39	14119.0
40	16288.0
41	15358.0
42	14428.0
43	16363.0
44	18298.0
45	20151.5
46	22005.0
47	23610.5
48	25216.0
49	28118.0
50	31020.0
51	31790.0
52	32560.0
53	34377.5
54	36195.0
55	40269.5
56	44344.0
57	48111.0
58	51878.0
59	58507.0
60	65136.0
61	64404.0
62	63672.0
63	62806.5
64	61941.0
65	51835.0
66	41729.0
67	36541.0
68	31353.0
69	26392.0
70	21431.0
71	19244.5
72	17058.0
73	13182.0
74	9306.0
75	8401.0
76	7496.0
77	5583.0
78	3670.0
79	2230.5
80	791.0
81	573.0
82	355.0
83	217.0
84	79.0
85	44.0
86	9.0
87	5.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	665873.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.948524718677586
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.18694854327444	8.74476063753899
2	9.479399713431034	2.265296835883119
3	3.311882556999573	1.1871633179299956
4	1.4655237424901335	0.7004338665180898
5	0.8471380809934391	0.50610251504416
6	0.5379452502450919	0.38565912719092077
7	0.37329378346446795	0.3122216999337712
8	0.29033960936125286	0.27753039994112993
9	0.19355973957416858	0.20814779995584742
>10	5.994067519670194	20.515323492617963
>50	2.8707171765415653	23.637991028319213
>100	1.3662301098514367	31.093016235828753
>500	0.060330308438701886	4.75706328383941
>1k	0.02136698423870692	4.593218226298408
>5k	0.0012568814258062894	0.8160715331602273
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5434	0.8160715331602273	No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	4495	0.6750536513719583	No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	4080	0.6127294544154815	No Hit
TGGATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	1933	0.2902955969081191	No Hit
TGGATGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1802	0.27062217570017105	No Hit
TGGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	1766	0.2652157393376815	No Hit
TGGATGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	1617	0.24283909994848868	No Hit
TGGATGGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACT	1572	0.23608105449537675	No Hit
TGGATGGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTA	1566	0.23517998176829516	No Hit
TGGATGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	1550	0.23277712116274424	No Hit
TGGATGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	1540	0.23127533328427494	No Hit
TGGATGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1434	0.21535638177250016	No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	1391	0.2088986938950821	No Hit
TGGATGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAA	1342	0.20153993329058242	No Hit
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	1316	0.19763528480656223	No Hit
TGGATGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCA	1109	0.16654827572224734	No Hit
TGGATGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	1042	0.1564862969365029	No Hit
TGGATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTA	1030	0.15468415148233972	No Hit
TGGATGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	969	0.1455232454236769	No Hit
TGGATGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA	943	0.14161859693965667	No Hit
TGGATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC	919	0.1380143060313303	No Hit
TGGATGGGGGAAGAGTCGGCCGCTGCAGGCGTGCGCGCACTGTTTGCTGC	887	0.1332085848202285	No Hit
TGGATGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	885	0.1329082272445346	No Hit
TGGATGGGGTCTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTAT	878	0.1318569757296061	No Hit
TGGATGGGGGGCTGATGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCT	878	0.1318569757296061	No Hit
TGGATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	843	0.1266007181549635	No Hit
TGGATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	838	0.12584982421572882	No Hit
TGGATGGGGGGGCTGGTGAGATGGCTTAGTGGGTAAGAGCACCCGACTGC	760	0.11413587876366814	No Hit
TGGATGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTCC	744	0.11173301815811723	No Hit
TGGATGGGGATCTGAACAAGACAATTTGGCTCTGCCTAGTGTTCTGTGCC	743	0.11158283937027032	No Hit
TGGATGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	729	0.10948033634041326	No Hit
TGGATGGGGCTGTAGAACGCAAGAAGACAGCTGTGGTTCTAAGTGCTAAA	713	0.10707747573486236	No Hit
TGGATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	691	0.10377354240222986	No Hit
TGGATGGGGGGCACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTC	687	0.10317282725084212	No Hit
TGGATGGGGACCTTTGTTCCTGTTGCATTGTGGGACATTCCTCGGAGGAA	683	0.10257211209945441	No Hit
TGGATGGGTGCCCAGCGTGGTGGTGCACGCCTTTAATCCCAGCACTCAGG	679	0.10197139694806667	No Hit
TGGATGGGGATTTTATCACAGGTGAGACCAAGGACCAGGTTGCTAACTCC	668	0.10031943028175042	No Hit
TGGATGGGGATAAGAGTCGGCCGCTGCAGGCGTACGCGCACTGTTTGCTG	667	0.1001692514939035	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	6.00715151387727E-4	0.0	0.0	0.0	0.0
8	0.0015017878784693178	0.0	0.0	0.0	0.0
9	0.0034541121204794308	0.0	0.0	0.0	0.0
10	0.025980930297519196	0.0	0.0	0.0	0.0
11	0.05076043029226294	0.0	0.0	0.0	0.0
12	0.06367580604709908	0.0	0.0	0.0	0.0
13	0.07283671210576191	0.0	0.0	0.0	0.0
14	0.0831990484672002	0.0	0.0	0.0	0.0
15	0.09671513937342406	0.0	0.0	0.0	0.0
16	0.10887962118902554	0.0	0.0	0.0	0.0
17	0.11999285148969849	0.0	0.0	0.0	0.0
18	0.13275804845668768	0.0	0.0	0.0	0.0
19	0.14822646360492167	0.0	0.0	0.0	0.0
20	0.15903933632990075	0.0	0.0	0.0	0.0
21	0.17135399693334916	0.0	0.0	0.0	0.0
22	0.18396901511249142	0.0	0.0	0.0	0.0
23	0.19928725147287846	0.0	0.0	0.0	0.0
24	0.21295352116694927	0.0	0.0	0.0	0.0
25	0.22857211510303016	0.0	0.0	0.0	0.0
26	0.24178784843356016	0.0	0.0	0.0	0.0
27	0.25500358176409016	0.0	0.0	0.0	0.0
28	0.27062217570017105	0.0	0.0	0.0	0.0
29	0.28323719387931334	0.0	0.0	0.0	0.0
30	0.2945006029678332	0.0	0.0	0.0	0.0
31	0.30471276054142454	0.0	0.0	0.0	0.0
32	0.31507509690286284	0.0	0.0	0.0	0.0
33	0.3285911878090867	0.0	0.0	0.0	0.0
34	0.34015495447330046	0.0	0.0	0.0	0.0
35	0.35697497871215683	0.0	0.0	0.0	0.0
36	0.3704910696183807	0.0	0.0	0.0	0.0
37	0.3819046574947475	0.0	0.0	0.0	0.0
38	0.39301788779542046	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAA	30	2.5261052E-6	44.000004	17
TAGGTGA	30	2.5261052E-6	44.000004	21
AGGCGTA	60	0.0	44.000004	28
CGATTCA	30	2.5261052E-6	44.000004	42
CCGTACC	30	2.5261052E-6	44.000004	12
TGCGCAA	30	2.5261052E-6	44.000004	14
GTGTACG	30	2.5261052E-6	44.000004	32
CCGAAAT	30	2.5261052E-6	44.000004	44
CACGTAA	60	0.0	44.000004	28
CGCGTAT	30	2.5261052E-6	44.000004	15
CCTATAC	30	2.5261052E-6	44.000004	21
CGATCTC	65	0.0	44.000004	21
GCGTATA	30	2.5261052E-6	44.000004	16
GTTATCC	30	2.5261052E-6	44.000004	33
GCATAAC	25	4.440816E-5	44.0	9
TCACGCC	50	2.7284841E-11	44.0	18
TTTAGCG	20	7.8540936E-4	44.0	14
AAGTAGT	20	7.8540936E-4	44.0	17
ACCGGTA	20	7.8540936E-4	44.0	19
CAAACGC	25	4.440816E-5	44.0	32
>>END_MODULE