FastQCFastQC Report
Fri 17 Jun 2016
SRR1527536_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527536_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences507881
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC41600.8190895111256378No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA38090.7499788336244121No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT31960.629281268643639No Hit
TGGATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG17320.3410247676128857No Hit
TGGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC14760.2906192592359234No Hit
TGGATGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT12800.25202754188481163No Hit
TGGATGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT12740.25084616278222654No Hit
TGGATGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG12550.24710512895737388No Hit
TGGATGGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT12500.24612064637188633No Hit
TGGATGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA11860.23351926927764577No Hit
TGGATGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC11530.22702168421342797No Hit
TGGATGGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC10940.21540478970467491No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC10810.21284513498240729No Hit
TGGATGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGG10590.2085134116062621No Hit
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT9970.19630582754621653No Hit
TGGATGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG8860.17445031414839304No Hit
TGGATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT8860.17445031414839304No Hit
TGGATGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG7390.14550652613505918No Hit
TGGATGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG7150.14078100972471896No Hit
TGGATGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT7110.13999342365632894No Hit
TGGATGGGGGAAGAGTCGGCCGCTGCAGGCGTGCGCGCACTGTTTGCTGCTT6920.13625238983147628No Hit
TGGATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG6750.13290514904081863No Hit
TGGATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC6690.13172376993823356No Hit
TGGATGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA6490.1277858395962834No Hit
TGGATGGGGGGCTGATGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCT6420.1264075639766008No Hit
TGGATGGGGTCTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATCT6400.12601377094240582No Hit
TGGATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC6130.12069756498077305No Hit
TGGATGGGGCTGTAGAACGCAAGAAGACAGCTGTGGTTCTAAGTGCTAAAGC6110.12030377194657804No Hit
TGGATGGGGATAAGAGTCGGCCGCTGCAGGCGTACGCGCACTGTTTGCTGCT6090.11990997891238302No Hit
TGGATGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTCCCC6010.11833480677560294No Hit
TGGATGGGGACCTTTGTTCCTGTTGCATTGTGGGACATTCCTCGGAGGAAGT5900.11616894508753035No Hit
TGGATGGGGATTTTATCACAGGTGAGACCAAGGACCAGGTTGCTAACTCCGC5850.1151844625020428No Hit
TGGATGGGGGGCACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTA5830.11479066946784779No Hit
TGGATGGGGGGGCTGGTGAGATGGCTTAGTGGGTAAGAGCACCCGACTGCTC5750.11321549733106771No Hit
TGGATGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG5620.1106558426088001No Hit
TGGATGGGGATCTGAACAAGACAATTTGGCTCTGCCTAGTGTTCTGTGCCCT5410.1065210157497524No Hit
TGGATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA5370.10573342968136236No Hit
TGGATGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG5270.10376446451038727No Hit
TGGATGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCTG5160.10159860282231467No Hit
TGGATGGGTGCCCAGCGTGGTGGTGCACGCCTTTAATCCCAGCACTCAGGAG5090.1002203272026321No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCAAA351.0180702E-746.00000439
TTCAACG351.0180702E-746.00000419
CACGTAA351.0180702E-746.00000428
GTTGACG253.414444E-546.019
TCCATTA501.6370905E-1146.038
TCGTTGC551.8189894E-1246.045
GGTACGA405.6006684E-946.07
ACCGGTA253.414444E-546.019
ACGTTTA206.308112E-446.014
AGATACG206.308112E-446.010
CACGGAA206.308112E-446.041
CGTTGCG206.308112E-446.043
TTATGCG301.8593364E-646.014
TTCACCG301.8593364E-646.036
CGATTTG405.6006684E-946.025
TAAGTAC405.6006684E-946.011
TAAAACG253.414444E-546.017
TATGCGC301.8593364E-646.015
ATACTCT405.6006684E-946.026
ATCGTAA253.414444E-546.018
Produced by FastQC (version 0.11.5)