##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527536_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	507881
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.9123475774837	33.0	31.0	33.0	31.0	34.0
2	32.30212392272993	34.0	31.0	34.0	31.0	34.0
3	32.97860522445218	34.0	33.0	34.0	31.0	34.0
4	34.342438484605644	35.0	35.0	35.0	32.0	37.0
5	35.57928924295258	35.0	35.0	37.0	35.0	37.0
6	36.18036705448717	37.0	36.0	37.0	35.0	37.0
7	36.5875372380538	37.0	37.0	37.0	35.0	37.0
8	36.685331800165784	37.0	37.0	37.0	35.0	37.0
9	38.51822966403547	39.0	39.0	39.0	38.0	39.0
10	37.467231497142045	39.0	37.0	39.0	34.0	39.0
11	36.72014901128414	37.0	35.0	39.0	34.0	39.0
12	36.7353198879265	39.0	35.0	39.0	33.0	39.0
13	36.796568487500025	39.0	35.0	39.0	33.0	39.0
14	37.45400792705378	40.0	35.0	40.0	33.0	40.0
15	37.63839954635043	40.0	35.0	40.0	33.0	40.0
16	37.79569426696411	40.0	35.0	40.0	34.0	40.0
17	37.71941655624054	40.0	35.0	40.0	34.0	40.0
18	37.587454541516614	40.0	35.0	40.0	34.0	40.0
19	37.48001992592753	40.0	35.0	40.0	33.0	40.0
20	37.38707098710131	40.0	35.0	40.0	33.0	40.0
21	37.39606718896749	40.0	35.0	40.0	33.0	40.0
22	37.3737824411624	40.0	35.0	40.0	33.0	40.0
23	37.30348644662825	39.0	35.0	40.0	33.0	40.0
24	37.26575122912651	39.0	35.0	40.0	33.0	40.0
25	37.2341394933065	39.0	35.0	40.0	33.0	40.0
26	37.240058202610456	39.0	35.0	40.0	33.0	40.0
27	37.142931119691426	39.0	35.0	40.0	33.0	40.0
28	37.098617589553456	39.0	35.0	40.0	33.0	40.0
29	37.00553476109561	39.0	35.0	40.0	33.0	40.0
30	36.8791823281438	39.0	35.0	40.0	33.0	40.0
31	36.83813531122448	39.0	35.0	40.0	32.0	40.0
32	36.76297597271802	39.0	35.0	40.0	33.0	40.0
33	36.566475217619875	38.0	35.0	40.0	32.0	40.0
34	36.127541687915084	38.0	35.0	40.0	31.0	40.0
35	36.00297313740817	38.0	35.0	40.0	31.0	40.0
36	35.91654738019339	38.0	35.0	40.0	30.0	40.0
37	35.83553430823362	38.0	35.0	40.0	30.0	40.0
38	36.004615254360765	38.0	35.0	40.0	31.0	40.0
39	35.94798781604352	38.0	35.0	40.0	31.0	40.0
40	35.67704048783081	38.0	35.0	40.0	30.0	40.0
41	35.60555130040305	37.0	34.0	40.0	30.0	40.0
42	35.547134072745386	37.0	34.0	40.0	30.0	40.0
43	35.54607870741374	37.0	34.0	40.0	30.0	40.0
44	35.39951484698187	37.0	34.0	40.0	30.0	40.0
45	35.309668997265106	36.0	34.0	40.0	30.0	40.0
46	35.12931375656896	36.0	34.0	40.0	29.0	40.0
47	34.949326712359785	36.0	34.0	40.0	29.0	40.0
48	34.773507967417565	35.0	34.0	40.0	28.0	40.0
49	34.69721450497262	35.0	34.0	39.0	28.0	40.0
50	35.04923003616989	35.0	34.0	40.0	30.0	40.0
51	35.157668823996175	35.0	34.0	40.0	30.0	40.0
52	34.80581868587326	35.0	34.0	39.0	30.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	8.0
14	9.0
15	19.0
16	42.0
17	83.0
18	145.0
19	224.0
20	325.0
21	525.0
22	796.0
23	1117.0
24	1604.0
25	2156.0
26	3190.0
27	4308.0
28	5167.0
29	6215.0
30	8039.0
31	11531.0
32	16083.0
33	27761.0
34	60537.0
35	36439.0
36	47128.0
37	83884.0
38	160121.0
39	30422.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	98.84106710036407	0.8553184702715794	0.1972903101317041	0.10632411923265489
8	98.99917500359336	0.7799071042232334	0.11419997991655526	0.10671791226684992
9	93.89286072918657	3.203900126210667	1.8622472587082408	1.0409918858945304
10	55.56793815874192	30.8985766350779	5.708817616725178	7.82466758945501
11	36.32622602538784	24.172394714509895	20.955893211204987	18.54548604889728
12	32.1000391824069	17.949874084677315	25.2866321047647	24.663454628151083
13	26.360899502048706	18.845753237470984	27.769890978398482	27.023456282081824
14	24.057013355490756	19.270065231816112	30.785361137746836	25.887560274946296
15	27.357195878562102	19.373829696326503	28.0453492058179	25.223625219293496
16	32.62240564226659	20.40871779019101	23.86523614783778	23.103640419704615
17	30.710934254283977	21.52787759337325	23.274152803511058	24.487035348831714
18	31.56566203500426	20.83243909498485	22.660229463201027	24.941669406809865
19	28.99714696946726	22.865001840982437	22.276084358343784	25.861766831206523
20	30.403184998060567	23.18003626833845	22.084504047207908	24.33227468639307
21	30.089135053290043	21.30577832208726	24.27182745564414	24.33325916897856
22	29.741415804095837	20.833423577570336	25.531374475516905	23.89378614281692
23	30.049558853353442	20.72808394092317	24.95210492221603	24.270252283507357
24	29.054050062908438	21.021853544432652	23.773285474353244	26.150810918305666
25	29.344669322144362	21.810817888442372	21.438486574610984	27.40602621480229
26	28.36550294261845	21.635973781259786	23.14124765447024	26.857275621651528
27	26.300058478265576	21.01890009667619	23.243436946843847	29.437604478214386
28	27.297930026915758	23.31510727906734	21.747220313419877	27.63974238059703
29	28.613395657644215	23.088676284405203	22.20697368084256	26.090954377108023
30	29.66620133456459	20.981095965393468	24.044215081879415	25.308487618162523
31	30.26397916047263	21.801760648655886	21.995506821479836	25.938753369391648
32	33.41176377931051	20.29254884510348	22.199688509709954	24.095998865876062
33	34.04143884098834	21.42706657661933	22.109313008362193	22.422181574030137
34	28.282609508920398	22.881738044935723	25.47722793331509	23.358424512828794
35	29.446858614517968	26.145494712344032	23.282225560712057	21.125421112425943
36	28.743938048479862	26.936624918041822	21.850000295344778	22.46943673813354
37	30.281699847011406	25.2626107296788	21.51074759638577	22.944941826924023
38	31.75074476107592	23.90953786418472	20.826729095989023	23.51298827875034
39	31.723966834750662	21.71276342292781	20.16889783236624	26.394371909955282
40	28.28536606015976	22.960496651774726	22.053394397506505	26.70074289055901
41	27.39145587253707	24.054453700768487	21.5711948271347	26.982895599559743
42	26.643642900600735	21.550323796322367	23.01858112431849	28.78745217875841
43	26.511131544594107	20.34354504303173	25.301793136581207	27.84353027579295
44	25.891301308771148	19.68551688289186	24.97907974505839	29.444102063278603
45	28.151082635499257	20.551664661603798	24.246230908421463	27.051021794475478
46	26.93760940062731	23.080209734170012	22.454669499351226	27.52751136585145
47	26.38590535972009	21.968138205603278	25.897995790352464	25.747960644324163
48	26.65329082993851	22.708469109889915	25.628050665411777	25.010189394759795
49	29.198375209940913	22.061664051224597	22.596434991661432	26.143525747173058
50	25.838926835223212	22.477312598817438	24.656957043086866	27.026803522872484
51	24.747726337468816	23.57993309456349	23.57678275028993	28.095557817677765
52	25.136793855253494	22.614943264268597	25.20590453275472	27.042358347723187
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.5
6	3.0
7	3.0
8	3.0
9	25.0
10	47.0
11	157.5
12	268.0
13	284.0
14	343.0
15	386.0
16	452.0
17	518.0
18	560.5
19	603.0
20	559.0
21	515.0
22	661.5
23	808.0
24	1092.0
25	1376.0
26	1645.0
27	1914.0
28	2126.5
29	2339.0
30	2888.0
31	3437.0
32	3708.5
33	3980.0
34	4286.0
35	4592.0
36	7429.5
37	10267.0
38	10115.0
39	10128.0
40	10293.0
41	10555.5
42	10818.0
43	12045.0
44	13272.0
45	14557.0
46	15842.0
47	17270.0
48	18698.0
49	20972.5
50	23247.0
51	23520.5
52	23794.0
53	25191.5
54	26589.0
55	28857.0
56	31125.0
57	35564.0
58	40003.0
59	42898.5
60	45794.0
61	46003.0
62	46212.0
63	47344.0
64	42245.0
65	36014.0
66	29602.5
67	23191.0
68	19886.5
69	16582.0
70	15223.5
71	13865.0
72	12180.5
73	10496.0
74	8374.0
75	6252.0
76	5290.0
77	4328.0
78	2696.0
79	1064.0
80	716.5
81	369.0
82	274.5
83	180.0
84	108.0
85	36.0
86	25.5
87	15.0
88	10.0
89	3.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	507881.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.60071158401279
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.1357566076636	7.3288821594034825
2	9.058489199279334	1.9205286277691036
3	3.289437025204777	1.0461111953390656
4	1.4487639072048144	0.6143171333442282
5	0.7745314734671892	0.4105292381483064
6	0.520069094894036	0.33078614872381523
7	0.49220825052471256	0.3652430392158793
8	0.3937666004197701	0.3339364929973754
9	0.3380449116811234	0.3225164950057198
>10	10.085625661695055	29.265910715305353
>50	2.962536451271383	21.285694877343314
>100	1.4246178420847342	28.433038447982895
>500	0.050149519864782034	3.419108019398245
>1k	0.026003454744701796	4.923397410023214
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4160	0.8190895111256378	No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	3809	0.7499788336244121	No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	3196	0.629281268643639	No Hit
TGGATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	1732	0.3410247676128857	No Hit
TGGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1476	0.2906192592359234	No Hit
TGGATGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT	1280	0.25202754188481163	No Hit
TGGATGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	1274	0.25084616278222654	No Hit
TGGATGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1255	0.24710512895737388	No Hit
TGGATGGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT	1250	0.24612064637188633	No Hit
TGGATGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	1186	0.23351926927764577	No Hit
TGGATGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1153	0.22702168421342797	No Hit
TGGATGGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC	1094	0.21540478970467491	No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	1081	0.21284513498240729	No Hit
TGGATGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGG	1059	0.2085134116062621	No Hit
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	997	0.19630582754621653	No Hit
TGGATGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG	886	0.17445031414839304	No Hit
TGGATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT	886	0.17445031414839304	No Hit
TGGATGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	739	0.14550652613505918	No Hit
TGGATGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	715	0.14078100972471896	No Hit
TGGATGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	711	0.13999342365632894	No Hit
TGGATGGGGGAAGAGTCGGCCGCTGCAGGCGTGCGCGCACTGTTTGCTGCTT	692	0.13625238983147628	No Hit
TGGATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG	675	0.13290514904081863	No Hit
TGGATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	669	0.13172376993823356	No Hit
TGGATGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	649	0.1277858395962834	No Hit
TGGATGGGGGGCTGATGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCT	642	0.1264075639766008	No Hit
TGGATGGGGTCTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATCT	640	0.12601377094240582	No Hit
TGGATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	613	0.12069756498077305	No Hit
TGGATGGGGCTGTAGAACGCAAGAAGACAGCTGTGGTTCTAAGTGCTAAAGC	611	0.12030377194657804	No Hit
TGGATGGGGATAAGAGTCGGCCGCTGCAGGCGTACGCGCACTGTTTGCTGCT	609	0.11990997891238302	No Hit
TGGATGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTCCCC	601	0.11833480677560294	No Hit
TGGATGGGGACCTTTGTTCCTGTTGCATTGTGGGACATTCCTCGGAGGAAGT	590	0.11616894508753035	No Hit
TGGATGGGGATTTTATCACAGGTGAGACCAAGGACCAGGTTGCTAACTCCGC	585	0.1151844625020428	No Hit
TGGATGGGGGGCACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTA	583	0.11479066946784779	No Hit
TGGATGGGGGGGCTGGTGAGATGGCTTAGTGGGTAAGAGCACCCGACTGCTC	575	0.11321549733106771	No Hit
TGGATGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	562	0.1106558426088001	No Hit
TGGATGGGGATCTGAACAAGACAATTTGGCTCTGCCTAGTGTTCTGTGCCCT	541	0.1065210157497524	No Hit
TGGATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	537	0.10573342968136236	No Hit
TGGATGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG	527	0.10376446451038727	No Hit
TGGATGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCTG	516	0.10159860282231467	No Hit
TGGATGGGTGCCCAGCGTGGTGGTGCACGCCTTTAATCCCAGCACTCAGGAG	509	0.1002203272026321	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.937930341950181E-4	0.0	0.0	0.0	0.0
8	0.0015751721367800724	0.0	0.0	0.0	0.0
9	0.004331723376145199	0.0	0.0	0.0	0.0
10	0.028943788013333832	0.0	0.0	0.0	0.0
11	0.051193094445352354	0.0	0.0	0.0	0.0
12	0.06773240188154311	0.0	0.0	0.0	0.0
13	0.07856171032190612	0.0	0.0	0.0	0.0
14	0.08939101876226911	0.0	0.0	0.0	0.0
15	0.10474894709587482	0.0	0.0	0.0	0.0
16	0.12148515104916309	0.0	0.0	0.0	0.0
17	0.13566170028018373	0.0	0.0	0.0	0.0
18	0.1498382495112044	0.0	0.0	0.0	0.0
19	0.16440859177642006	0.0	0.0	0.0	0.0
20	0.17464721066549055	0.0	0.0	0.0	0.0
21	0.18311376090068343	0.0	0.0	0.0	0.0
22	0.19413996585814394	0.0	0.0	0.0	0.0
23	0.20772582553787206	0.0	0.0	0.0	0.0
24	0.21796444442694254	0.0	0.0	0.0	0.0
25	0.22879375286730552	0.0	0.0	0.0	0.0
26	0.23804788917088845	0.0	0.0	0.0	0.0
27	0.24671133592317884	0.0	0.0	0.0	0.0
28	0.2585251269490294	0.0	0.0	0.0	0.0
29	0.2671885737013198	0.0	0.0	0.0	0.0
30	0.27683650303909774	0.0	0.0	0.0	0.0
31	0.2841216741717056	0.0	0.0	0.0	0.0
32	0.29219443137270346	0.0	0.0	0.0	0.0
33	0.3002671885737013	0.0	0.0	0.0	0.0
34	0.31188408308245436	0.0	0.0	0.0	0.0
35	0.3309830452409127	0.0	0.0	0.0	0.0
36	0.3414185606470807	0.0	0.0	0.0	0.0
37	0.35244476560454124	0.0	0.0	0.0	0.0
38	0.3628802810107092	0.0	0.0	0.0	0.0
39	0.37213441731429214	0.0	0.0	0.0	0.0
40	0.3800102779981925	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCAAA	35	1.0180702E-7	46.000004	39
TTCAACG	35	1.0180702E-7	46.000004	19
CACGTAA	35	1.0180702E-7	46.000004	28
GTTGACG	25	3.414444E-5	46.0	19
TCCATTA	50	1.6370905E-11	46.0	38
TCGTTGC	55	1.8189894E-12	46.0	45
GGTACGA	40	5.6006684E-9	46.0	7
ACCGGTA	25	3.414444E-5	46.0	19
ACGTTTA	20	6.308112E-4	46.0	14
AGATACG	20	6.308112E-4	46.0	10
CACGGAA	20	6.308112E-4	46.0	41
CGTTGCG	20	6.308112E-4	46.0	43
TTATGCG	30	1.8593364E-6	46.0	14
TTCACCG	30	1.8593364E-6	46.0	36
CGATTTG	40	5.6006684E-9	46.0	25
TAAGTAC	40	5.6006684E-9	46.0	11
TAAAACG	25	3.414444E-5	46.0	17
TATGCGC	30	1.8593364E-6	46.0	15
ATACTCT	40	5.6006684E-9	46.0	26
ATCGTAA	25	3.414444E-5	46.0	18
>>END_MODULE