FastQCFastQC Report
Fri 17 Jun 2016
SRR1527535_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527535_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences694543
Sequences flagged as poor quality0
Sequence length52
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC55560.7999504710291516No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA49730.7160103838063302No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT41030.5907481610209879No Hit
TGGATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG21840.3144513730611352No Hit
TGGATGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT18150.26132291305217964No Hit
TGGATGGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT17510.25210822080130385No Hit
TGGATGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT17140.24678097684376635No Hit
TGGATGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC16900.2433254672496879No Hit
TGGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC16760.24130975331980883No Hit
TGGATGGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC16680.24015791678844936No Hit
TGGATGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA16040.23094322453757363No Hit
TGGATGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG16000.2303673062718939No Hit
TGGATGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGG14750.21236986046940218No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC13810.19883578122592843No Hit
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT13440.19350853726839087No Hit
TGGATGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG12240.17623098929799882No Hit
TGGATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT11280.1624089509216852No Hit
TGGATGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG10310.1484429329789516No Hit
TGGATGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT10260.14772303514685195No Hit
TGGATGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG10100.14541936208413303No Hit
TGGATGGGGGAAGAGTCGGCCGCTGCAGGCGTGCGCGCACTGTTTGCTGCTT9650.138940281595236No Hit
TGGATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG9360.13476487416905794No Hit
TGGATGGGGTCTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATCT9300.13390099677053832No Hit
TGGATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC9230.1328931398055988No Hit
TGGATGGGGGGCTGATGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCT8960.1290056915122606No Hit
TGGATGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA8850.12742191628164132No Hit
TGGATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC8740.12583814105102203No Hit
TGGATGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG8390.12079885622632437No Hit
TGGATGGGGGGGCTGGTGAGATGGCTTAGTGGGTAAGAGCACCCGACTGCTC8340.12007895839422468No Hit
TGGATGGGGCTGTAGAACGCAAGAAGACAGCTGTGGTTCTAAGTGCTAAAGC7640.11000038874482933No Hit
TGGATGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTCCCC7530.10841661351421006No Hit
TGGATGGGGACCTTTGTTCCTGTTGCATTGTGGGACATTCCTCGGAGGAAGT7500.10798467481495026No Hit
TGGATGGGGATCTGAACAAGACAATTTGGCTCTGCCTAGTGTTCTGTGCCCT7450.1072647769828506No Hit
TGGATGGGTGCCCAGCGTGGTGGTGCACGCCTTTAATCCCAGCACTCAGGAG7370.10611294045149113No Hit
TGGATGGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA7280.10481712435371172No Hit
TGGATGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCTG7270.1046731447872918No Hit
TGGATGGGGATAAGAGTCGGCCGCTGCAGGCGTACGCGCACTGTTTGCTGCT7210.10380926738877219No Hit
TGGATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA7190.10352130825593232No Hit
TGGATGGGGATTGAGCGCCCAACCTACACTAACCTAAACAGGTTGATAGGTC7120.10251345129099279No Hit
TGGATGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG7090.10208151259173297No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTT206.30972E-446.00000414
CAAGTAA206.30972E-446.00000428
CATTCAA206.30972E-446.00000423
ATCCGTT206.30972E-446.00000430
GTACTAA206.30972E-446.00000440
CGAATAC206.30972E-446.00000437
GCCTACT206.30972E-446.00000422
CGTAATA206.30972E-446.00000429
TTAGTCG206.30972E-446.00000433
TAGGATA206.30972E-446.00000422
CGGTATA206.30972E-446.00000440
ACATATC206.30972E-446.00000425
CATCATA206.30972E-446.00000424
CGTTTAC206.30972E-446.00000430
CAATACG206.30972E-446.00000432
ATACCGA206.30972E-446.00000416
ACGCGTC206.30972E-446.00000446
AACCGTA301.8603314E-646.022
TAATAGG253.4157485E-546.024
GATACTC453.092282E-1046.09
Produced by FastQC (version 0.11.5)