##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527535_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	694543
Sequences flagged as poor quality	0
Sequence length	52
%GC	56
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.067435709524105	31.0	31.0	33.0	30.0	34.0
2	31.425943390114075	31.0	31.0	34.0	30.0	34.0
3	32.237842725360416	34.0	31.0	34.0	30.0	34.0
4	33.28523647923887	35.0	33.0	35.0	28.0	37.0
5	34.824319012645724	35.0	35.0	37.0	33.0	37.0
6	35.61554000256284	37.0	35.0	37.0	35.0	37.0
7	36.35030948407802	37.0	35.0	37.0	35.0	37.0
8	36.5134988042497	37.0	37.0	37.0	35.0	37.0
9	38.3976658032692	39.0	39.0	39.0	37.0	39.0
10	36.86413800153482	39.0	37.0	39.0	32.0	39.0
11	36.00057735806134	37.0	35.0	39.0	32.0	39.0
12	36.01102019601378	38.0	35.0	39.0	31.0	39.0
13	36.00822267303824	38.0	35.0	39.0	32.0	39.0
14	36.69819867164452	39.0	35.0	40.0	32.0	40.0
15	36.90134952047605	39.0	35.0	40.0	32.0	40.0
16	37.052319294845674	39.0	35.0	40.0	33.0	40.0
17	37.012464311064974	39.0	35.0	40.0	33.0	40.0
18	36.91275990111483	39.0	35.0	40.0	32.0	40.0
19	36.760583577978615	39.0	35.0	40.0	32.0	40.0
20	36.60693290408225	39.0	35.0	40.0	31.0	40.0
21	36.6972181707972	39.0	35.0	40.0	31.0	40.0
22	36.68728214091856	39.0	35.0	40.0	31.0	40.0
23	36.714633939151355	39.0	35.0	40.0	32.0	40.0
24	36.71739978662228	38.0	35.0	40.0	32.0	40.0
25	36.67703655497212	38.0	35.0	40.0	32.0	40.0
26	36.62741111781416	38.0	35.0	40.0	32.0	40.0
27	36.522375144519486	38.0	35.0	40.0	32.0	40.0
28	36.396015797438025	38.0	35.0	40.0	31.0	40.0
29	36.328669355245104	38.0	35.0	40.0	31.0	40.0
30	36.27277648756088	38.0	35.0	40.0	31.0	40.0
31	36.222543456632636	38.0	35.0	40.0	31.0	40.0
32	36.145626692659775	38.0	35.0	40.0	31.0	40.0
33	35.98529536688153	38.0	35.0	40.0	31.0	40.0
34	35.622138586091864	38.0	34.0	40.0	30.0	40.0
35	35.508370252093826	38.0	34.0	40.0	29.0	40.0
36	35.47218674725683	37.0	34.0	40.0	29.0	40.0
37	35.46546866068768	37.0	34.0	40.0	29.0	40.0
38	35.3753532898611	37.0	34.0	40.0	29.0	40.0
39	35.24357599169526	37.0	34.0	40.0	29.0	40.0
40	35.395130323104546	37.0	34.0	40.0	29.0	40.0
41	35.471279675988384	37.0	34.0	40.0	30.0	40.0
42	35.56188889672778	37.0	34.0	40.0	30.0	40.0
43	35.54457823345711	37.0	34.0	40.0	30.0	40.0
44	35.449331430883326	37.0	34.0	40.0	30.0	40.0
45	35.37273430154792	36.0	34.0	40.0	30.0	40.0
46	35.13128488804869	36.0	34.0	40.0	30.0	40.0
47	35.02915874179136	35.0	34.0	40.0	29.0	40.0
48	34.954470781506686	35.0	34.0	40.0	29.0	40.0
49	34.88849502478608	35.0	34.0	39.0	29.0	40.0
50	34.72809314902029	35.0	34.0	39.0	29.0	40.0
51	34.60139113057075	35.0	34.0	39.0	29.0	40.0
52	34.13722116557218	35.0	33.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	4.0
14	13.0
15	26.0
16	65.0
17	115.0
18	229.0
19	370.0
20	614.0
21	975.0
22	1509.0
23	2217.0
24	3107.0
25	4052.0
26	5640.0
27	7498.0
28	9544.0
29	11531.0
30	14753.0
31	20820.0
32	29259.0
33	45805.0
34	84286.0
35	53204.0
36	73762.0
37	120300.0
38	183405.0
39	21439.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	98.87076826056847	0.8434323000879715	0.1958122103311098	0.08998722901245855
8	99.03965629197904	0.7727383329757841	0.09848202343123463	0.08912335161393896
9	93.96178494348082	3.1832442339783134	1.8426504910423112	1.0123203314985536
10	55.376700938602795	31.042426458836964	5.618946559104332	7.961926043455912
11	35.878268156183275	24.26991561357612	21.23065094601774	18.62116528422286
12	31.807389895226066	18.178140158348725	25.412105513985455	24.60236443243975
13	26.191034968317297	18.766296687174155	27.87660375239546	27.166064592113088
14	23.96827842192636	19.15029019081612	30.884336894907875	25.99709449234965
15	27.256627739391227	19.23739782850018	28.2683721526241	25.237602279484495
16	32.165611056478866	20.512480868715112	23.97374964545032	23.348158429355706
17	30.577372459300577	21.45007580524172	23.31662690430974	24.655924831147964
18	31.40482302751593	20.771068170005314	22.627108760724678	25.197000041754077
19	28.856240722316688	22.84667759951508	22.250170255837293	26.046911422330943
20	30.060197856720173	23.14053989457816	22.317552692921822	24.481709555779844
21	29.892317682274534	21.135624432180585	24.57500831481996	24.397049570724924
22	29.75841668550399	20.784170310549527	25.615404661770402	23.842008342176076
23	29.799450861933675	20.76185347775444	24.971815999873296	24.46687966043859
24	28.87970939164314	21.186161259993984	23.783408658643165	26.150720689719716
25	29.144919752988656	22.04096794582913	21.35735296446728	27.45675933671493
26	28.257861644275444	21.97171377438114	23.138092241949025	26.632332339394395
27	26.40542054271658	21.10265311147042	23.155945708185094	29.335980637627905
28	27.368787821632356	23.387896789687606	21.775037686651512	27.468277702028526
29	28.63883157702259	23.07920459928327	22.174149044767567	26.107814778926574
30	29.3611770617514	21.161396774569752	24.10059564346628	25.37683052021257
31	30.059046020188813	21.95098071681667	21.91081041778551	26.07916284520901
32	33.30319361076276	20.403344357368802	22.202944958051553	24.090517073816883
33	33.55256621980209	21.59923863605277	22.211871691169588	22.636323452975553
34	28.11431401655477	23.10699265560232	25.432694591983505	23.345998735859407
35	29.26528667051572	26.0032856137057	23.42590739522247	21.305520320556106
36	28.59966913495637	27.055920223801838	21.81477604698341	22.529634594258383
37	30.215263849754443	25.067130472843296	21.70808718826624	23.00951848913602
38	31.498409745688893	23.918461491945063	20.853424481997514	23.72970428036853
39	31.319587124195337	21.77604554361645	20.286288969869396	26.61807836231882
40	28.216683488279344	23.022332670547396	21.913546029547486	26.847437811625774
41	27.17311959086766	24.278554387561318	21.838388695876283	26.70993732569474
42	26.381231975558027	21.670652500997058	23.09720204508576	28.850913478359153
43	26.447606555677616	20.465543530062213	25.409225922657058	27.677623991603113
44	25.71604637869794	19.9735653516053	24.92214304945842	29.388245220238346
45	27.821603558023046	20.66351543388962	24.567665356932544	26.94721565115479
46	27.10084184852486	23.05616786865608	22.479529705144248	27.363460577674815
47	26.128835795623885	21.95069275768383	26.051518768456383	25.868952678235907
48	26.641547031645267	22.955382172162125	25.42391183843189	24.979158957760713
49	29.130377816780246	22.16003904725841	22.690171810816608	26.019411325144738
50	25.77637381702789	22.647697838722728	24.548804033731532	27.027124310517852
51	24.55369933898981	23.57895767432686	23.716458160257897	28.150884826425433
52	25.154382090093776	22.781454855926846	25.011122421505938	27.05304063247344
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	2.0
7	2.0
8	2.0
9	32.0
10	62.0
11	207.0
12	352.0
13	370.5
14	459.0
15	529.0
16	602.0
17	675.0
18	724.5
19	774.0
20	738.0
21	702.0
22	924.5
23	1147.0
24	1670.5
25	2194.0
26	2481.0
27	2768.0
28	3078.0
29	3388.0
30	4130.0
31	4872.0
32	5172.5
33	5473.0
34	5878.5
35	6284.0
36	10418.0
37	14552.0
38	14114.0
39	14043.0
40	14410.0
41	14749.5
42	15089.0
43	16807.0
44	18525.0
45	20183.0
46	21841.0
47	23725.0
48	25609.0
49	28981.5
50	32354.0
51	32473.0
52	32592.0
53	34495.0
54	36398.0
55	39449.0
56	42500.0
57	48568.5
58	54637.0
59	58261.0
60	61885.0
61	62050.0
62	62215.0
63	63871.5
64	57070.0
65	48612.0
66	39955.0
67	31298.0
68	27041.5
69	22785.0
70	21149.0
71	19513.0
72	16827.5
73	14142.0
74	11358.0
75	8574.0
76	7210.0
77	5846.0
78	3715.0
79	1584.0
80	1032.5
81	481.0
82	361.5
83	242.0
84	133.5
85	25.0
86	19.5
87	14.0
88	8.5
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	694543.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.951723651379396
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.99205717675314	7.064933344659726
2	9.435900403651672	1.878069464381615
3	3.5286968850822498	1.053498487494655
4	1.7072006250090426	0.679583553502087
5	0.8608342134579493	0.4283392100993027
6	0.5367554507443685	0.3204985148507724
7	0.38050319015032047	0.2650663817790979
8	0.3486740259552366	0.27759260405763214
9	0.2329316107003863	0.20862639174248387
>10	6.536552901517672	18.68739588477603
>50	3.5778874115655603	24.866566936820327
>100	1.7534975911109825	33.23465933714687
>500	0.0795729104877096	5.282754271513787
>1k	0.027488823623026955	4.9524651461464595
>5k	0.0014467801906856291	0.7999504710291516
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	5556	0.7999504710291516	No Hit
TGGATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	4973	0.7160103838063302	No Hit
TGGATGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4103	0.5907481610209879	No Hit
TGGATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	2184	0.3144513730611352	No Hit
TGGATGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCT	1815	0.26132291305217964	No Hit
TGGATGGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT	1751	0.25210822080130385	No Hit
TGGATGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATT	1714	0.24678097684376635	No Hit
TGGATGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	1690	0.2433254672496879	No Hit
TGGATGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	1676	0.24130975331980883	No Hit
TGGATGGGGGACTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATC	1668	0.24015791678844936	No Hit
TGGATGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	1604	0.23094322453757363	No Hit
TGGATGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1600	0.2303673062718939	No Hit
TGGATGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGG	1475	0.21236986046940218	No Hit
TGGATGGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	1381	0.19883578122592843	No Hit
TGGATGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	1344	0.19350853726839087	No Hit
TGGATGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAG	1224	0.17623098929799882	No Hit
TGGATGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACT	1128	0.1624089509216852	No Hit
TGGATGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	1031	0.1484429329789516	No Hit
TGGATGGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	1026	0.14772303514685195	No Hit
TGGATGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1010	0.14541936208413303	No Hit
TGGATGGGGGAAGAGTCGGCCGCTGCAGGCGTGCGCGCACTGTTTGCTGCTT	965	0.138940281595236	No Hit
TGGATGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG	936	0.13476487416905794	No Hit
TGGATGGGGTCTGTTTGCTGCTTTAGGTGTCTCTTTTCCTCCCTCTCTATCT	930	0.13390099677053832	No Hit
TGGATGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	923	0.1328931398055988	No Hit
TGGATGGGGGGCTGATGAGATGGCTCAGCAGGTAAGAGCACCCGACTGCTCT	896	0.1290056915122606	No Hit
TGGATGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	885	0.12742191628164132	No Hit
TGGATGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	874	0.12583814105102203	No Hit
TGGATGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	839	0.12079885622632437	No Hit
TGGATGGGGGGGCTGGTGAGATGGCTTAGTGGGTAAGAGCACCCGACTGCTC	834	0.12007895839422468	No Hit
TGGATGGGGCTGTAGAACGCAAGAAGACAGCTGTGGTTCTAAGTGCTAAAGC	764	0.11000038874482933	No Hit
TGGATGGGGGCCCCAGTCACTGAGCCGCCGCCGAGGACTCAGCTGCCTCCCC	753	0.10841661351421006	No Hit
TGGATGGGGACCTTTGTTCCTGTTGCATTGTGGGACATTCCTCGGAGGAAGT	750	0.10798467481495026	No Hit
TGGATGGGGATCTGAACAAGACAATTTGGCTCTGCCTAGTGTTCTGTGCCCT	745	0.1072647769828506	No Hit
TGGATGGGTGCCCAGCGTGGTGGTGCACGCCTTTAATCCCAGCACTCAGGAG	737	0.10611294045149113	No Hit
TGGATGGGGGCTCTTTCTAAGCCGGCGATCCGTCAGGGTGAAGCCATGTTCA	728	0.10481712435371172	No Hit
TGGATGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCTG	727	0.1046731447872918	No Hit
TGGATGGGGATAAGAGTCGGCCGCTGCAGGCGTACGCGCACTGTTTGCTGCT	721	0.10380926738877219	No Hit
TGGATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	719	0.10352130825593232	No Hit
TGGATGGGGATTGAGCGCCCAACCTACACTAACCTAAACAGGTTGATAGGTC	712	0.10251345129099279	No Hit
TGGATGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACG	709	0.10208151259173297	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	5.759182656797347E-4	0.0	0.0	0.0	0.0
8	0.0014397956641993368	0.0	0.0	0.0	0.0
9	0.004175407426178077	0.0	0.0	0.0	0.0
10	0.021740914529409985	0.0	0.0	0.0	0.0
11	0.0423299925274605	0.0	0.0	0.0	0.0
12	0.054424276106734934	0.0	0.0	0.0	0.0
13	0.06291907052551102	0.0	0.0	0.0	0.0
14	0.07342957887416618	0.0	0.0	0.0	0.0
15	0.08451600548850108	0.0	0.0	0.0	0.0
16	0.0983380438648147	0.0	0.0	0.0	0.0
17	0.1098564091784094	0.0	0.0	0.0	0.0
18	0.11979099926138483	0.0	0.0	0.0	0.0
19	0.1334690580712785	0.0	0.0	0.0	0.0
20	0.14498742338487322	0.0	0.0	0.0	0.0
21	0.15549793173352838	0.0	0.0	0.0	0.0
22	0.1658644605157636	0.0	0.0	0.0	0.0
23	0.17795874409503804	0.0	0.0	0.0	0.0
24	0.18890119114295298	0.0	0.0	0.0	0.0
25	0.19941169949160814	0.0	0.0	0.0	0.0
26	0.20977822827384338	0.0	0.0	0.0	0.0
27	0.22129659358743808	0.0	0.0	0.0	0.0
28	0.23310291803387262	0.0	0.0	0.0	0.0
29	0.24260556941758826	0.0	0.0	0.0	0.0
30	0.25354801646550323	0.0	0.0	0.0	0.0
31	0.2616108721850195	0.0	0.0	0.0	0.0
32	0.26780199354107664	0.0	0.0	0.0	0.0
33	0.27687270622553245	0.0	0.0	0.0	0.0
34	0.28536750064430855	0.0	0.0	0.0	0.0
35	0.3020691303490209	0.0	0.0	0.0	0.0
36	0.312579638697676	0.0	0.0	0.0	0.0
37	0.32251422878065145	0.0	0.0	0.0	0.0
38	0.3307210640665877	0.0	0.0	0.0	0.0
39	0.3405116745831432	0.0	0.0	0.0	0.0
40	0.3477106529041398	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGTT	20	6.30972E-4	46.000004	14
CAAGTAA	20	6.30972E-4	46.000004	28
CATTCAA	20	6.30972E-4	46.000004	23
ATCCGTT	20	6.30972E-4	46.000004	30
GTACTAA	20	6.30972E-4	46.000004	40
CGAATAC	20	6.30972E-4	46.000004	37
GCCTACT	20	6.30972E-4	46.000004	22
CGTAATA	20	6.30972E-4	46.000004	29
TTAGTCG	20	6.30972E-4	46.000004	33
TAGGATA	20	6.30972E-4	46.000004	22
CGGTATA	20	6.30972E-4	46.000004	40
ACATATC	20	6.30972E-4	46.000004	25
CATCATA	20	6.30972E-4	46.000004	24
CGTTTAC	20	6.30972E-4	46.000004	30
CAATACG	20	6.30972E-4	46.000004	32
ATACCGA	20	6.30972E-4	46.000004	16
ACGCGTC	20	6.30972E-4	46.000004	46
AACCGTA	30	1.8603314E-6	46.0	22
TAATAGG	25	3.4157485E-5	46.0	24
GATACTC	45	3.092282E-10	46.0	9
>>END_MODULE