FastQCFastQC Report
Fri 17 Jun 2016
SRR1527534_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527534_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52060
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17093.2827506723011908No Hit
TGTGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12732.4452554744525545No Hit
TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8771.6845946984248943No Hit
TGTGCGGGGGCCCGCACGCCCGGATGGCTCAACGCACCCGGCGCGTACAG5100.9796388782174413No Hit
TGTGCGGGGAGTACAACCGGCGCTTTGTCAATGTTGTGCCCACCTTTGGG4930.9469842489435267No Hit
TGTGCGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4840.9296965040338072No Hit
TGTGCGGGGACAGCTCTGTGCACATCTGCCCCCTCCCCCCCTCACAGCCA4140.7952362658470996No Hit
TGTGCGGCCCCATAGCAAAGGAAACCATAAACAAAACAAAAAGACAACCT3330.6396465616596235No Hit
TGTGCGGGGGCTTGTGCGGGGGCTTGGTTCTAAGAAACAGCTGGTCAGTA3200.6146753745678064No Hit
TGTGCGGGGGGGGAGACAAGGCCCCAAAGAAGGAAGTCAGCTCCCTTGGG3150.6050710718401844No Hit
TGTGCGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3140.6031502112946601No Hit
TGTGCGGGGGATCAGCTGAAGGTTCATGGGTTTTAAGTGCTTGTGGCTCT2940.5647330003841721No Hit
TGTGCGGGGTCCCAAGCAATGGAGAAATCTCCTTGCCCATCATTGCTGCT2840.5455243949289282No Hit
TGTGCGGGGGGGGATTTTCAGGACAGAGAAAGTACTTTGAATAAGACTGC2810.539761813292355No Hit
TGTGCGGGGGCAAAGATGGAGATCTGTGACCTCCTGCCTAATGGCGAATT2790.5359200922013062No Hit
TGTGCGGGGAATTTCTGCAGAGGATATTGGTAACTTACCAAAGAGAAGCA2790.5359200922013062No Hit
TGTGCGGGGTCAAATTAAATTAAACTTAACATAATTAATTTCTAGACATC2690.5167114867460622No Hit
TGTGCGGGGGACCACTTCACTCAGCCTTGCCCATCAGAGGAAAAACAAAG2510.4821359969266231No Hit
TGTGCGGGACTCTTGTTGAATATCAATAAGATGTTTTCCCAGAGTTTGCT2510.4821359969266231No Hit
TGTGCGGGGAACTGGTTAGGTTTTCTATTCTTTGTTTCTTTCTTTTTTTT2480.4763734152900499No Hit
TGTGCGGGGGTGCTTGTGCGGGGGACCCTAACTCCCAAAGGTGCCCCTAA2450.4706108336534768No Hit
TGTGCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGTGCGGGGA2400.46100653092585475No Hit
TGTGCGGGGGGGGGGGAGGCCCCTCCCACCTCCAGTTTCCCTGACTTCTC2350.45140222819823284No Hit
TGTGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2350.45140222819823284No Hit
TGTGCGGGGTACACAAGTTGAAAGCATAAATAATGTGAACTTAAGAAGTC2320.4456396465616596No Hit
TGTGCGGGTCACCTACCTCACGCAAATTCCTGACGGATAAAAATGTTGAT2260.4341144832885132No Hit
TGTGCGGAGATGTGAAAGGCTGACTAGTTGAGGGTTTAGCTGCAGGGAAG2170.4168267383787937No Hit
TGTGCGGGGGAATTTTCTCTGCTCCTTGAATATCTTCACTAGTTTGTTCC2130.4091432961966961No Hit
TGTGCGGGGTCCAGTGGTTAAGACTCTGCCTTCCACTCCAGGGGTTGCGG2110.4053015751056473No Hit
TGTGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2060.3956972723780253No Hit
TGTGCGGGGGAAATATAGATCAATGGAACAAAATAGAAAGCCCAGAGATA2020.3880138301959278No Hit
TGTGCGGGGAAGGCTGCTGTTCTGTGTCACAGTGAGTAATGAGGATTTAG1990.3822512485593546No Hit
TGTGCGGGGTGTCAGAGCGGAACATTCTCAGCCGCCTGGCAAACAGGGCG1970.3784095274683058No Hit
TGTGCGGGGAGTTTGAAAAAGGAAATGAGGAGAAAAGTATTTGTACTGTA1850.35535920092201306No Hit
TGTGCGGGGCACCAGAACACTGGCGAGAATGATAAGCTAAGATTTGAAAT1790.34383403764886666No Hit
TGTGCGGGGATGGCCAAGTGGAGTTCCCCCAGAGGGGGAGGTGGGGTAAG1760.3380714560122935No Hit
TGTGCGGGGATCAACTCTGCTTCTCTTCTTCTGTATTAGGGTTGGGTTTT1750.3361505954667691No Hit
TGTGCGGGGCTCAGCCGTGTCCGACTCTTTGTGACCCCATGGACAACACA1640.31502112946600075No Hit
TGTGCGGGGACATTTACTTTTCATTAAGTATCAATATTCTAATAAATACA1630.3131002689204764No Hit
TGTGCGGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1620.31117940837495195No Hit
TGTGCGGGGGGGGTTCAGGATGGGGAGCACATGTATACCTGTGATGATTT1570.30157510564733003No Hit
TGTGCGGGGGTCGGAACAGTGGCGTAATGGCGAGTTGATCTGGGCCGTGA1550.2977333845562812No Hit
TGTGCGGGGATTAAAAGGCATGTGCCACCACCCCCAGCTTTTGTTGAAAA1500.28812908182865926No Hit
TGTGCGGGGGTGCTTGTGCGGGGTTGTGCGGGGGGGAAAAATTAGCTCAT1460.28044563964656166No Hit
TGTGCGGGGGGGGGGAGGCCCCTCCCACCTCCAGTTTCCCTGACTTCTCA1450.27852477910103723No Hit
TGTGCGGGGGTGCAGTGCTTGTGCGGGGATGAATGTTCATTACAATAAAA1440.27660391855551286No Hit
TGTGCGGGGGTGCAGTGCTTGTGCGGGGAAAAAAAAAAAAAAAAAAAAAA1390.2669996158278909No Hit
TGTGCGGGGTGACTCGTACCAGTTTGGAACTATTTACCCGGATCTGTAAC1360.2612370341913177No Hit
TGTGCGGGGCCCAGCACTCAGGAGGCAGAGGCAGGTGAATTTCTGAGTTC1360.2612370341913177No Hit
TGTGCGGGGTGACCGGTTAGACCTAATTGATATCTGTAGGACATTTCACC1350.25931617364579335No Hit
TGTGCGGGGGTGAGTTCATCTCTATACCCATCTCTGATGAAGGTCTGAGT1340.2573953131002689No Hit
TGTGCGGGGGAAAAGATGTAAACAATAGAGCGCGCTCTGCGCTTTGGCGC1340.2573953131002689No Hit
TGTGCGGGGAAGGACAGTGCTGTGTGAAGGTGGAGTACCCCAGAGGCCGG1290.24779101037264695No Hit
TGTGCGGGGAAATCACTGTTGGCTCAAGAAAATTCTTTGGATTCTGATAA1240.23818670764502495No Hit
TGTGCGGGTACATCATACACAGAACTTAACTCAAACTGGATAAAAAAATT1230.23626584709950058No Hit
TGTGCGGGGATTTTAAAAAAATGAAAAATTAATTTAAAAAAATGCAAAAA1210.2324241260084518No Hit
TGTGCGGGGAAGCACTGCTACTGTCCTCCATGACAACTTTTTTTTTTTGG1210.2324241260084518No Hit
TGTGCGGGAGCTCTGCCGGCCCGAGAGGGGCGGGCACTTTTTCAGCTTGC1170.22474068382635418No Hit
TGTGCGGGGCTCATCACATAATATCAATGAAAGTCCACATGTATGGATAC1140.21897810218978103No Hit
TGTGCGGGGGAGTGCAGTGCTCTGTGTGGGGAGCCCTGGGCCCCACAACT1140.21897810218978103No Hit
TGTGCGGGGAGGGCACTTTACAGGGGGTCGTGGTGGCTTCAAGATCCTCC1060.20361121782558586No Hit
TGTGCGGGTGAGACCTCACTCTTTGGGATCAAACACACATGGGCCGTGAG1050.20169035728006146No Hit
TGTGCGGGAGAACTTGTTTTTGTTGATAGTATTTGCTTTCTAGAACTTGT1040.1997694967345371No Hit
TGTGCGGGGGCTTGTGCAAGCGGTCAGAGTTGCGTCATGGGACAGACGCG1040.1997694967345371No Hit
TGTGCGGGGGATTGCCATCTGTCCTAGCTCTACCTGCCTCAAGTTGAAAT1010.1940069150979639No Hit
TGTGCGGGTAAGGCGGCCCTGGCATTGGCTTGGTGATTACTGGCTGCACT1010.1940069150979639No Hit
TGTGCGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA990.19016519400691512No Hit
TGTGCGGGGTATTTGCTAGGATGTCACAATCTTTATTTTAGGTCTTCTCA980.1882443334613907No Hit
TGTGCGGGGTTTCTTCCAATTATCTTCAGAATCTCAAAAAACTAATTTAC950.18248175182481752No Hit
TGTGCGGGGAACAATAAACAAAAAGGAATGGCCTGGGTCTCTGTTTTATA940.18056089127929312No Hit
TGTGCGGGGATGAATTAAATGAATTAACAATGTTCATAAACATGCCACCA940.18056089127929312No Hit
TGTGCGGGGAAGGAAATGTATTTTGACACTGATATCTTATTAAAGTATTC910.17479830964271995No Hit
TGTGCGGGGGAGTGCAGTGCTTGTGCGGGGAGACTGGGCTGGATCCAAAC890.17095658855167115No Hit
TGTGCGGGGTCCCTAGCCTCGCCTCACATCGGCCCAGCCTGGGCTGGATC870.16711486746062237No Hit
TGTGCGGGGAGCCCATTGTCCTATTCTGATCTCCCCCGCAGGCCCAGGAT860.16519400691509797No Hit
TGTGCGGGGAATGTTGATGAATCAGCATATTCCTGCAGTAATAAAGAAAT860.16519400691509797No Hit
TGTGCGGGGGAGCAGTGATATCAACGCAGAGTGCAGTGCCTTTGCGGGGG860.16519400691509797No Hit
TGTGCGGGGAGATGGTACTCAGATAAAGCGAAACTAGCAACAGTTTCTGG800.1536688436419516No Hit
TGTGCGGGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.1517479830964272No Hit
TGTGCGGGAGAGTCACCCAGCTCCAGGCCCCTGGCCACTTTGGTACTTGG780.1498271225509028No Hit
TGTGCGGTTGCGGGGTGGTGGCTCACAACCATCCGTAGCGAGATCTGACT770.1479062620053784No Hit
TGTGCGGGGCTTGTGCGGGGGTGCTTGTGCGGGAAGCAGTGGTATCAACG770.1479062620053784No Hit
TGTGCGGGGGTGAGTAAGCTTTGCAGTTCCTGTGAATTCGTGTCTCTTGG770.1479062620053784No Hit
TGTGCGGGTTATCACGGGTGGATTCAGGCAACCCTACCCATCCTCACTGT760.145985401459854No Hit
TGTGCGGGGCTGACTGGCTTTTATTTTCCTTTTCAAAATCAGGTTTTTGT760.145985401459854No Hit
TGTGCGGGGACTTCAAAATAGGAAAAAGCCACCTCCCCATAGAAAGAACT750.14406454091432963No Hit
TGTGCGGGGGGTGCCATGCTCCATGCCTATAATCCCAGCATTGAGAGGCC740.14214368036880523No Hit
TGTGCGGGGAAAGAGGTGATTATCAAAAACATGTTTGAAATCCTGATGAA740.14214368036880523No Hit
TGTGCGGGGAGGACTTCCCTGATAGCTCAGTTGGTGAAGAATCTGCCTGC740.14214368036880523No Hit
TGTGCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA730.14022281982328083Illumina Single End Adapter 2 (97% over 34bp)
TGTGCGGGGCACCTTAGCAAAGCATCAAGATCAGGTTTGATCCTCCACAT730.14022281982328083No Hit
TGTGCGGGGATGTTGAAGAAAGAATCTTGACTCCAGAACCAAAACCTTAG730.14022281982328083No Hit
TGTGCGGGGAGACGCAGGTTTGATCCCTGGGTTGGGAAGATCCCTGGAGA720.13830195927775643No Hit
TGTGCGGGGAAGTCCTAGAGTCCTGGGATCCTGGCCTGCTCCTTGCCAGC710.13638109873223206No Hit
TGTGCGGGGAACCAATCAAAATGAAGAATTGTGGAGCCCAGTTCCAACAG710.13638109873223206No Hit
TGTGCGGGGGCAGTGCTTGTGCGGGGCTTGTGCGGGGAGTGGTATCAGTG710.13638109873223206No Hit
TGTGCGGGGAGGAGAAAAAGGAGGTAGTAAGGGAAAAAGGGCTCTCAGTG700.13446023818670766No Hit
TGTGCGGGGCAACAAGCATCATGGGCACAGACACGTCTATGAGGTGGAAG700.13446023818670766No Hit
TGTGCGGGGGTGCTTGTGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA690.13253937764118323No Hit
TGTGCGGGGGAGAAGCTGATGAATCACTACAGTGTAAATAAAACCTTTTT680.13061851709565886No Hit
TGTGCGGGGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.13061851709565886No Hit
TGTGCGGGGAGCCTGAGTGTTAGGAAAACATCGCTCAGAACTATTGATTC670.12869765655013446No Hit
TGTGCGGGGATTCACCTGCGGCCAATGTTCCAGGCGATTCGCTGGCCGGT660.12677679600461006No Hit
TGTGCGGGGAGATAGGAATGCACACATTTGCATACTTAGATAGAAACACA660.12677679600461006No Hit
TGTGCGGGGGTGCAGTGCTTGTGCGGGGGAAAAAAAAAAAAAAAAAAAAA650.12485593545908567No Hit
TGTGCGGGGGTGTGTGTGTGTTTTAAATGAGGTCTGCAACCAAAGGCAGG650.12485593545908567No Hit
TGTGCGGGGGGGGGGGGAGGCCCCTCCCACCTCCAGTTTCCCTGACTTCT640.12293507491356129No Hit
TGTGCGGGGAGAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.12101421436803689No Hit
TGTGCGGGGCATGTGGTTGCTGGGAATTGAACTCAGGACCTCTGGAAGAA630.12101421436803689No Hit
TGTGCGGGGCTGGGAACTTATGGATATATAACTCATACATCTCCTTTGTG630.12101421436803689No Hit
TGTGCGGGTCAGTGAATACAGAAAGAGCATCTCGCCCTGGGAAGAAGTAC630.12101421436803689No Hit
TGTGCGGGGATCAGTTTACTATTTATTGTTGAGGGGGCTCAACTTTCTAA620.11909335382251247No Hit
TGTGCGGGGGGTCGCTTCTGGCTCCTTCCCAGGTGGGCTAGCATCTGTGA610.11717249327698809No Hit
TGTGCGGGGCAGAAGGCTTCTCCTCACAGGGCACTGTGTAACCATGAAAG610.11717249327698809No Hit
TGTGCGGGGATTCACATATCCACATAAATATAGCCCCAACACACACACAC600.11525163273146369No Hit
TGTGCGGGGGCAGTGCTTGTGCGGGGGGCAAAAAAAAAAAAAAAAAAAAA600.11525163273146369No Hit
TGTGCGGGGGAGTGCAGTGCTTGTGCGGGGAAAAAAAAAAAAAAAAAAAA600.11525163273146369No Hit
TGTGCGGGGTCCATTCTGTTCTCTTAGGCTTAGCTGGGTTGCCTGAGTCT580.1114099116404149No Hit
TGTGCGGGTGTCCACCTCCTTTCCAAAGCCTCCTGCTTCCAGGTCTCAGT580.1114099116404149No Hit
TGTGCGGGGATCAGCAGGAAGTAGACCAACAACGCCTCAGAAGATAGTCA580.1114099116404149No Hit
TGTGCGGGGAGTGCTTGTGCGGGGCTTTTCCCCTGCCGCCGCCGACTTGC580.1114099116404149No Hit
TGTGCGGGGCAGAGTGCAGTGCTTGTGCGGGAAAAAAAAAAAAAAAAAAA580.1114099116404149No Hit
TGTGCGGGTAGTCTGAGTTACCCATGTATCTTAAAAGTATTGAGACTTAG580.1114099116404149No Hit
TGTGCGGGGGCTACTAGGGGTCTTCCTCCCTCCGCCATGGACAAATTTTC570.10948905109489052No Hit
TGTGCGGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.10948905109489052No Hit
TGTGCGGGGAGCCCCCTAAAAATAATGTCTGTCACTATTTCCATTGTTTC560.10756819054936612No Hit
TGTGCGGGGAGCATTTCATTAAAATTTCAGATGCAAGGACAAAAAAAAAA540.10372646945831733No Hit
TGTGCGGGGATGGTGATCGTTGCTGTTCTGGGTGTCCTTGGAGCTATGGC540.10372646945831733No Hit
TGTGCGGGGCGCTGACAGTCAAAAGTCTCAAGGAAGAAAAGGAGGAATTC530.10180560891279293No Hit
TGTGCGGGGGTGCAGTGCTTGTGCGGGGGGAAAAAAAAAAAAAAAAAAAA530.10180560891279293No Hit
TGTGCGGGAGGATTTCCAAGGCAAGAATACCCGAGTGGGTTGTGATTGCC530.10180560891279293No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCCAT302.4812762E-644.0000047
TTGTTTC302.4812762E-644.00000432
TGTGAAG302.4812762E-644.00000423
ACAAAAT302.4812762E-644.00000428
ACAAAAC302.4812762E-644.00000431
TGGTTAG302.4812762E-644.00000413
AAACCAT302.4812762E-644.00000422
CGGAGGA650.044.0000045
GAAACCA302.4812762E-644.00000421
AACTGGT302.4812762E-644.00000410
CCTAATG302.4812762E-644.00000437
TTTCTAT302.4812762E-644.00000422
GCCTAAT302.4812762E-644.00000436
TAAAAGG302.4812762E-644.00000412
AAATAGA302.4812762E-644.00000431
GCGGCCC302.4812762E-644.0000044
TGTTTCT302.4812762E-644.00000433
CCAGAGA302.4812762E-644.00000442
AGGAAAC302.4812762E-644.00000419
TCTATTC302.4812762E-644.00000424
Produced by FastQC (version 0.11.5)