FastQCFastQC Report
Fri 17 Jun 2016
SRR1527533_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527533_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40101
Sequences flagged as poor quality0
Sequence length52
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13003.2418144185930524No Hit
TGTGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9252.306675643998903No Hit
TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6741.6807560908705519No Hit
TGTGCGGGGAGTACAACCGGCGCTTTGTCAATGTTGTGCCCACCTTTGGGAA4061.0124435799605995No Hit
TGTGCGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3940.9825191391735867No Hit
TGTGCGGGGGCCCGCACGCCCGGATGGCTCAACGCACCCGGCGCGTACAGCA3910.9750380289768336No Hit
TGTGCGGGGACAGCTCTGTGCACATCTGCCCCCTCCCCCCCTCACAGCCAGC3010.7506047230742375No Hit
TGTGCGGGGGCTTGTGCGGGGGCTTGGTTCTAAGAAACAGCTGGTCAGTATC2790.6957432482980475No Hit
TGTGCGGGGGGGGAGACAAGGCCCCAAAGAAGGAAGTCAGCTCCCTTGGGTT2740.6832747313034588No Hit
TGTGCGGCCCCATAGCAAAGGAAACCATAAACAAAACAAAAAGACAACCTAC2600.6483628837186105No Hit
TGTGCGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2440.6084636293359268No Hit
TGTGCGGGGGGGGATTTTCAGGACAGAGAAAGTACTTTGAATAAGACTGCAG2300.5735517817510786No Hit
TGTGCGGGGTACACAAGTTGAAAGCATAAATAATGTGAACTTAAGAAGTCTA2290.5710580783521607No Hit
TGTGCGGGGGATCAGCTGAAGGTTCATGGGTTTTAAGTGCTTGTGGCTCTCT2230.5560958579586545No Hit
TGTGCGGGGGATTGCCATCTGTCCTAGCTCTACCTGCCTCAAGTTGAAATTA2220.5536021545597366No Hit
TGTGCGGGGGACCACTTCACTCAGCCTTGCCCATCAGAGGAAAAACAAAGAG2190.5461210443629835No Hit
TGTGCGGGGTCCCAAGCAATGGAGAAATCTCCTTGCCCATCATTGCTGCTGA2130.5311588239694771No Hit
TGTGCGGGGTCAAATTAAATTAAACTTAACATAATTAATTTCTAGACATCCG2120.5286651205705593No Hit
TGTGCGGGGAATTTCTGCAGAGGATATTGGTAACTTACCAAAGAGAAGCAAT2110.5261714171716416No Hit
TGTGCGGGGGTGCTTGTGCGGGGGACCCTAACTCCCAAAGGTGCCCCTAACT2070.5161966035759706No Hit
TGTGCGGGGGCAAAGATGGAGATCTGTGACCTCCTGCCTAATGGCGAATTAA2050.5112091967781353No Hit
TGTGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2030.5062217899802998No Hit
TGTGCGGGTCACCTACCTCACGCAAATTCCTGACGGATAAAAATGTTGATAG2000.4987406797835466No Hit
TGTGCGGGACTCTTGTTGAATATCAATAAGATGTTTTCCCAGAGTTTGCTGG1930.48128475599112247No Hit
TGTGCGGGGAACTGGTTAGGTTTTCTATTCTTTGTTTCTTTCTTTTTTTTTT1810.45136031520410963No Hit
TGTGCGGGGTCCAGTGGTTAAGACTCTGCCTTCCACTCCAGGGGTTGCGGGT1670.41644846761926135No Hit
TGTGCGGGGGAAATATAGATCAATGGAACAAAATAGAAAGCCCAGAGATAAA1670.41644846761926135No Hit
TGTGCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGTGCGGGGAAG1620.4039799506246727No Hit
TGTGCGGAGATGTGAAAGGCTGACTAGTTGAGGGTTTAGCTGCAGGGAAGGT1610.401486247225755No Hit
TGTGCGGGGCTCAGCCGTGTCCGACTCTTTGTGACCCCATGGACAACACAGT1570.39151143363008406No Hit
TGTGCGGGGGTCGGAACAGTGGCGTAATGGCGAGTTGATCTGGGCCGTGAGG1490.37156180643874215No Hit
TGTGCGGGGGAATTTTCTCTGCTCCTTGAATATCTTCACTAGTTTGTTCCAC1470.3665743996409067No Hit
TGTGCGGGGACATTTACTTTTCATTAAGTATCAATATTCTAATAAATACAAT1470.3665743996409067No Hit
TGTGCGGGGCACCAGAACACTGGCGAGAATGATAAGCTAAGATTTGAAATTT1450.3615869928430712No Hit
TGTGCGGGGTGTCAGAGCGGAACATTCTCAGCCGCCTGGCAAACAGGGCGCC1450.3615869928430712No Hit
TGTGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1440.3590932894441535No Hit
TGTGCGGGGAGTTTGAAAAAGGAAATGAGGAGAAAAGTATTTGTACTGTATA1360.3391436622528117No Hit
TGTGCGGGGATGGCCAAGTGGAGTTCCCCCAGAGGGGGAGGTGGGGTAAGTG1320.32916884865714074No Hit
TGTGCGGGTACATCATACACAGAACTTAACTCAAACTGGATAAAAAAATTGA1310.326675145258223No Hit
TGTGCGGGGATCAACTCTGCTTCTCTTCTTCTGTATTAGGGTTGGGTTTTTT1270.31670033166255207No Hit
TGTGCGGGGGGGGTTCAGGATGGGGAGCACATGTATACCTGTGATGATTTCA1260.3142066282636343No Hit
TGTGCGGGGAAGGCTGCTGTTCTGTGTCACAGTGAGTAATGAGGATTTAGAC1240.30921922146579883No Hit
TGTGCGGGGGAAAAGATGTAAACAATAGAGCGCGCTCTGCGCTTTGGCGCGC1240.30921922146579883No Hit
TGTGCGGGGATTAAAAGGCATGTGCCACCACCCCCAGCTTTTGTTGAAAATT1230.3067255180668811No Hit
TGTGCGGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1190.2967507044712102No Hit
TGTGCGGGGGTGCTTGTGCGGGGTTGTGCGGGGGGGAAAAATTAGCTCATGA1150.2867758908755393No Hit
TGTGCGGGGGTGCAGTGCTTGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAA1150.2867758908755393No Hit
TGTGCGGGGAAGGACAGTGCTGTGTGAAGGTGGAGTACCCCAGAGGCCGGTT1140.28428218747662154No Hit
TGTGCGGGGGTGAGTTCATCTCTATACCCATCTCTGATGAAGGTCTGAGTAG1120.27929478067878605No Hit
TGTGCGGGGGGGGGGAGGCCCCTCCCACCTCCAGTTTCCCTGACTTCTCAGA1070.2668262636841974No Hit
TGTGCGGGGTGACTCGTACCAGTTTGGAACTATTTACCCGGATCTGTAACAT1040.2593451534874442No Hit
TGTGCGGGAGCTCTGCCGGCCCGAGAGGGGCGGGCACTTTTTCAGCTTGCTA1030.2568514500885265No Hit
TGTGCGGGGCCCAGCACTCAGGAGGCAGAGGCAGGGGAATTTCTGAGTTCAA1020.25435774668960875No Hit
TGTGCGGGTGAGACCTCACTCTTTGGGATCAAACACACATGGGCCGTGAGGC950.2369018228971846No Hit
TGTGCGGGGGTTGGACATGATGATGTATGCCTTTAATCCCAGCTCATAGAAG930.23191441609934915No Hit
TGTGCGGGGGTGCAGTGCTTGTGCGGGGATGAATGTTCATTACAATAAAATG920.2294207127004314No Hit
TGTGCGGGAGAACTTGTTTTTGTTGATAGTATTTGCTTTCTAGAACTTGTTT910.22692700930151366No Hit
TGTGCGGGGTGACCGGTTAGACCTAATTGATATCTGTAGGACATTTCACCCC910.22692700930151366No Hit
TGTGCGGGGCTCATCACATAATATCAATGAAAGTCCACATGTATGGATACAA880.21944589910476048No Hit
TGTGCGGGGAAATCACTGTTGGCTCAAGAAAATTCTTTGGATTCTGATAAAT840.20947108550908952No Hit
TGTGCGGGTAAGGCGGCCCTGGCATTGGCTTGGTGATTACTGGCTGCACTCT820.20448367871125409No Hit
TGTGCGGGGCTGACTGGCTTTTATTTTCCTTTTCAAAATCAGGTTTTTGTTC820.20448367871125409No Hit
TGTGCGGGGATGAATTAAATGAATTAACAATGTTCATAAACATGCCACCAGT800.19949627191341862No Hit
TGTGCGGGGTATTTGCTAGGATGTCACAATCTTTATTTTAGGTCTTCTCATT790.19700256851450088No Hit
TGTGCGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA790.19700256851450088No Hit
TGTGCGGGGTTTCTTCCAATTATCTTCAGAATCTCAAAAAACTAATTTACTG790.19700256851450088No Hit
TGTGCGGGGGAGTGCAGTGCTCTGTGTGGGGAGCCCTGGGCCCCACAACTGC770.19201516171666544No Hit
TGTGCGGGGAACAATAAACAAAAAGGAATGGCCTGGGTCTCTGTTTTATAAG760.1895214583177477No Hit
TGTGCGGGGATTTTAAAAAAATGAAAAATTAATTTAAAAAAATGCAAAAAAC760.1895214583177477No Hit
TGTGCGGGGAGCCTGAGTGTTAGGAAAACATCGCTCAGAACTATTGATTCAA750.18702775491882995No Hit
TGTGCGGGGGCTTGTGCAAGCGGTCAGAGTTGCGTCATGGGACAGACGCGGG730.18204034812099448No Hit
TGTGCGGGGAGATGGTACTCAGATAAAGCGAAACTAGCAACAGTTTCTGGAA700.1745592379242413No Hit
TGTGCGGGGGTGCTTGTGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.17206553452532355No Hit
TGTGCGGGGAGGGCACTTTACAGGGGGTCGTGGTGGCTTCAAGATCCTCCAA690.17206553452532355No Hit
TGTGCGGGGAGCCCATTGTCCTATTCTGATCTCCCCCGCAGGCCCAGGATCT680.16957183112640584No Hit
TGTGCGGGGAGGAGAAAAAGGAGGTAGTAAGGGAAAAAGGGCTCTCAGTGCC670.1670781277274881No Hit
TGTGCGGGGCGCTGACAGTCAAAAGTCTCAAGGAAGAAAAGGAGGAATTCTT660.16458442432857037No Hit
TGTGCGGGGCTTGTGCGGGGGTGCTTGTGCGGGAAGCAGTGGTATCAACGCA660.16458442432857037No Hit
TGTGCGGGGAATGTTGATGAATCAGCATATTCCTGCAGTAATAAAGAAATGC650.16209072092965263No Hit
TGTGCGGGAGAGTCACCCAGCTCCAGGCCCCTGGCCACTTTGGTACTTGGTT640.15959701753073488No Hit
TGTGCGGGGTCCCTAGCCTCGCCTCACATCGGCCCAGCCTGGGCTGGATCCC640.15959701753073488No Hit
TGTGCGGGGGTGCAGTGCTTGTGCGGGGGGAAAAAAAAAAAAAAAAAAAAAA640.15959701753073488No Hit
TGTGCGGGGAAGCACTGCTACTGTCCTCCATGACAACTTTTTTTTTTTGGTC640.15959701753073488No Hit
TGTGCGGGGACTGTGATTTCCTTTTGGGTGTATTTTGCTAAGTGAAACTTGT630.15710331413181716No Hit
TGTGCGGGGATTCACCTGCGGCCAATGTTCCAGGCGATTCGCTGGCCGGTGG630.15710331413181716No Hit
TGTGCGGGGATGTTGAAGAAAGAATCTTGACTCCAGAACCAAAACCTTAGGG600.14962220393506398No Hit
TGTGCGGGGAAAGAGGTGATTATCAAAAACATGTTTGAAATCCTGATGAAAA600.14962220393506398No Hit
TGTGCGGGGGTATAATCCCAACCCTTGGGATGCTGAGGCAGGAGGACCGCTG590.14712850053614623No Hit
TGTGCGGGGCCCAGCACTCAGGAGGCAGAGGCAGGTGAATTTCTGAGTTCAA590.14712850053614623No Hit
TGTGCGGGGGGTGCCATGCTCCATGCCTATAATCCCAGCATTGAGAGGCCAA580.14463479713722852No Hit
TGTGCGGGGATGGTGATCGTTGCTGTTCTGGGTGTCCTTGGAGCTATGGCCA580.14463479713722852No Hit
TGTGCGGGGCACCTTAGCAAAGCATCAAGATCAGGTTTGATCCTCCACATTC580.14463479713722852No Hit
TGTGCGGGGGGGGGGGAGGCCCCTCCCACCTCCAGTTTCCCTGACTTCTCAG580.14463479713722852No Hit
TGTGCGGGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.14463479713722852No Hit
TGTGCGGGGGAGTGCAGTGCTTGTGCGGGGAGACTGGGCTGGATCCAAACTT580.14463479713722852No Hit
TGTGCGGTTGCGGGGTGGTGGCTCACAACCATCCGTAGCGAGATCTGACTCC580.14463479713722852No Hit
TGTGCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA560.13964739033939302Illumina Single End Adapter 2 (97% over 34bp)
TGTGCGGGGGCAGTGCTTGTGCGGGGGGCAAAAAAAAAAAAAAAAAAAAAAA560.13964739033939302No Hit
TGTGCGGGGAAGGAAATGTATTTTGACACTGATATCTTATTAAAGTATTCTG550.1371536869404753No Hit
TGTGCGGGGATCAGTTTACTATTTATTGTTGAGGGGGCTCAACTTTCTAATG540.13465998354155756No Hit
TGTGCGGGGATTCACATATCCACATAAATATAGCCCCAACACACACACACAC540.13465998354155756No Hit
TGTGCGGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA530.1321662801426398No Hit
TGTGCGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA530.1321662801426398Illumina Single End Adapter 2 (97% over 34bp)
TGTGCGGGTCAGTGAATACAGAAAGAGCATCTCGCCCTGGGAAGAAGTACAG530.1321662801426398No Hit
TGTGCGGGGGTGAGTAAGCTTTGCAGTTCCTGTGAATTCGTGTCTCTTGGAG530.1321662801426398No Hit
TGTGCGGGGAACTTAATGGAAGATTACAGTAACTCTAAATAAGCAAAACCAC520.1296725767437221No Hit
TGTGCGGGGGAGAAGCTGATGAATCACTACAGTGTAAATAAAACCTTTTTCT520.1296725767437221No Hit
TGTGCGGGGGCAGTGCTTGTGCGGGGCTTGTGCGGGGAGTGGTATCAGTGCT520.1296725767437221No Hit
TGTGCGGGGAGCCCCCTAAAAATAATGTCTGTCACTATTTCCATTGTTTCCC510.12717887334480438No Hit
TGTGCGGGGAGATAGGAATGCACACATTTGCATACTTAGATAGAAACACATT510.12717887334480438No Hit
TGTGCGGGTTATCACGGGTGGATTCAGGCAACCCTACCCATCCTCACTGTCA500.12468516994588665No Hit
TGTGCGGGGTATGGGCAAAAATCAATATTGAATCTTTATTGTTATCTAGCTG500.12468516994588665No Hit
TGTGCGGGGTCCATTCTGTTCTCTTAGGCTTAGCTGGGTTGCCTGAGTCTTC500.12468516994588665No Hit
TGTGCGGGGAGGACTTCCCTGATAGCTCAGTTGGTGAAGAATCTGCCTGCAA500.12468516994588665No Hit
TGTGCGGGGGAGTGCAGTGCTTGTGCGGGGAAAAAAAAAAAAAAAAAAAAAA500.12468516994588665No Hit
TGTGCGGGGGTGTGTGTGTGTTTTAAATGAGGTCTGCAACCAAAGGCAGGGC500.12468516994588665No Hit
TGTGCGGGGACTTCAAAATAGGAAAAAGCCACCTCCCCATAGAAAGAACTGC500.12468516994588665No Hit
TGTGCGGGGGTGCAGTGCTTGTGCGGGAAAAAAAAAAAAAAAAAAAAAAAAA490.12219146654696891No Hit
TGTGCGGGGCAGAGTGCAGTGCTTGTGCGGGGGCCTTCTCTAGGTAACCGTG490.12219146654696891No Hit
TGTGCGGGGATGTGAGTGTGCTGTCATACATACTGTCAGAAGAGAACTTCGA480.11969776314805117No Hit
TGTGCGGGGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.11969776314805117No Hit
TGTGCGGGGCTGGGAACTTATGGATATATAACTCATACATCTCCTTTGTGTA470.11720405974913345No Hit
TGTGCGGGGAAGTCCTAGAGTCCTGGGATCCTGGCCTGCTCCTTGCCAGCGG470.11720405974913345No Hit
TGTGCGGGGAGAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA460.1147103563502157No Hit
TGTGCGGGTGTCCACCTCCTTTCCAAAGCCTCCTGCTTCCAGGTCTCAGTGC460.1147103563502157No Hit
TGTGCGGGAATGTGATTTCTCCATACTGCCAAGGCTTCCGTGTGTAACATCC450.11221665295129797No Hit
TGTGCGGGGAAACGTTTCAAACCATGTAGAAGTGAATTAAGTAAAAAGTAAA450.11221665295129797No Hit
TGTGCGGGGATCAGCAGGAAGTAGACCAACAACGCCTCAGAAGATAGTCAGA450.11221665295129797No Hit
TGTGCGGGGCATGTGGTTGCTGGGAATTGAACTCAGGACCTCTGGAAGAAGT450.11221665295129797No Hit
TGTGCGGGGCAGGCATAGAGGCATAGATCCACATACCTATGGATATCCAATT450.11221665295129797No Hit
TGTGCGGGGCAACAAGCATCATGGGCACAGACACGTCTATGAGGTGGAAGTG450.11221665295129797No Hit
TGTGCGGGGATTCTTGCTTTTCAGCTGCTAATCACTGAGCCTTGGCTAGAGC440.10972294955238024No Hit
TGTGCGGGGGTGCAGTGCTTGTGCGGGGGAAAAAAAAAAAAAAAAAAAAAAA440.10972294955238024No Hit
TGTGCGGGGAGGTGGGTAGAGAGAGGTGAGGAGGACCTGGAAGAAGTTAGAG440.10972294955238024No Hit
TGTGCGGGGGGTCGCTTCTGGCTCCTTCCCAGGTGGGCTAGCATCTGTGAGT440.10972294955238024No Hit
TGTGCGGGGAGTGCTTGTGCGGGGCTTTTCCCCTGCCGCCGCCGACTTGCGC440.10972294955238024No Hit
TGTGCGGAGAGAGAAGGCCCTTCGAGATGCCAAGCTGGACAAGTCACAGATC430.1072292461534625No Hit
TGTGCGGGGCAGAGTGCAGTGCTTGTGCGGGAAAAAAAAAAAAAAAAAAAAA430.1072292461534625No Hit
TGTGCGGGGGCCACGTGGGAGGCGGAGGGAGGGAATAGGAAAGCTTCCTCCT430.1072292461534625No Hit
TGTGCGGGGATGCTGATTCCTGAATAAACTTCATGCGATATTGCATCAAAAA430.1072292461534625No Hit
TGTGCGGGGGGTTCCCGACCTTCCGGTCGCACCTCAGGATGAGGCCGGTCTC430.1072292461534625No Hit
TGTGCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.10473554275454476No Hit
TGTGCGGGGATGGTATGTGTGTTGTCTTAGACAGTGTTTTGTTGCTGTGAGC420.10473554275454476No Hit
TGTGCGGGGGTCCAAATGGCATTGGCCTGTGTTAGGAATCTCCATTTCTCGG410.10224183935562704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCACTC359.869473E-846.00000416
ACAAAGA359.869473E-846.00000445
AACAAAG359.869473E-846.00000444
GCCTTGC359.869473E-846.00000424
GGGTCAA359.869473E-846.0000047
ATAAGAC359.869473E-846.00000441
ACCACTT359.869473E-846.00000411
GACAGAG359.869473E-846.00000422
GGACCAC359.869473E-846.0000049
AGAGGAA359.869473E-846.00000436
GATGTGA359.869473E-846.0000049
CATCAGA359.869473E-846.00000432
ACTTCAC359.869473E-846.00000414
CCACTTC359.869473E-846.00000412
ACTCAGC359.869473E-846.00000419
TCAGCCT359.869473E-846.00000421
AAGACTG359.869473E-846.00000443
AGAAAGT359.869473E-846.00000427
CACTTCA359.869473E-846.00000413
GACTGCA359.869473E-846.00000445
Produced by FastQC (version 0.11.5)