FastQCFastQC Report
Fri 17 Jun 2016
SRR1527532_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527532_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53389
Sequences flagged as poor quality0
Sequence length52
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17563.289067036280882No Hit
TGTGCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12682.3750210717563545No Hit
TGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8811.6501526531682555No Hit
TGTGCGGGGGCCCGCACGCCCGGATGGCTCAACGCACCCGGCGCGTACAGCA5501.030174755099365No Hit
TGTGCGGGGAGTACAACCGGCGCTTTGTCAATGTTGTGCCCACCTTTGGGAA5411.0133173500159207No Hit
TGTGCGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5040.9440146846728728No Hit
TGTGCGGGGACAGCTCTGTGCACATCTGCCCCCTCCCCCCCTCACAGCCAGC3880.7267414635973702No Hit
TGTGCGGGGGCTTGTGCGGGGGCTTGGTTCTAAGAAACAGCTGGTCAGTATC3590.6724231583284946No Hit
TGTGCGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3400.6368353031523347No Hit
TGTGCGGCCCCATAGCAAAGGAAACCATAAACAAAACAAAAAGACAACCTAC3330.6237239880874337No Hit
TGTGCGGGACTCTTGTTGAATATCAATAAGATGTTTTCCCAGAGTTTGCTGG3190.5975013579576317No Hit
TGTGCGGGGGCAAAGATGGAGATCTGTGACCTCCTGCCTAATGGCGAATTAA3140.5881361329112739No Hit
TGTGCGGGGGACCACTTCACTCAGCCTTGCCCATCAGAGGAAAAACAAAGAG3140.5881361329112739No Hit
TGTGCGGGGGATCAGCTGAAGGTTCATGGGTTTTAAGTGCTTGTGGCTCTCT3130.5862630879020023No Hit
TGTGCGGGGTCCCAAGCAATGGAGAAATCTCCTTGCCCATCATTGCTGCTGA3020.565659592800015No Hit
TGTGCGGGGGGGGAGACAAGGCCCCAAAGAAGGAAGTCAGCTCCCTTGGGTT2940.5506752327258424No Hit
TGTGCGGGGGGGGATTTTCAGGACAGAGAAAGTACTTTGAATAAGACTGCAG2900.5431830526887561No Hit
TGTGCGGGGAACTGGTTAGGTTTTCTATTCTTTGTTTCTTTCTTTTTTTTTT2840.5319447826331267No Hit
TGTGCGGGGAATTTCTGCAGAGGATATTGGTAACTTACCAAAGAGAAGCAAT2820.5281986926145835No Hit
TGTGCGGGGGAATTTTCTCTGCTCCTTGAATATCTTCACTAGTTTGTTCCAC2730.5113412875311394No Hit
TGTGCGGGGTCAAATTAAATTAAACTTAACATAATTAATTTCTAGACATCCG2670.5001030174755099No Hit
TGTGCGGGGGTGCTTGTGCGGGGGACCCTAACTCCCAAAGGTGCCCCTAACT2650.49635692745696675No Hit
TGTGCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2600.4869917024106089No Hit
TGTGCGGGGTACACAAGTTGAAAGCATAAATAATGTGAACTTAAGAAGTCTA2600.4869917024106089No Hit
TGTGCGGGGATCAACTCTGCTTCTCTTCTTCTGTATTAGGGTTGGGTTTTTT2520.4720073423364364No Hit
TGTGCGGGGTCCAGTGGTTAAGACTCTGCCTTCCACTCCAGGGGTTGCGGGT2400.4495308022251775No Hit
TGTGCGGGGACATTTACTTTTCATTAAGTATCAATATTCTAATAAATACAAT2340.43829253216954805No Hit
TGTGCGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTTGTGCGGGGAAG2340.43829253216954805No Hit
TGTGCGGGGAAGGCTGCTGTTCTGTGTCACAGTGAGTAATGAGGATTTAGAC2300.43080035213246176No Hit
TGTGCGGAGATGTGAAAGGCTGACTAGTTGAGGGTTTAGCTGCAGGGAAGGT2270.425181217104647No Hit
TGTGCGGGTCACCTACCTCACGCAAATTCCTGACGGATAAAAATGTTGATAG2160.40457772200265973No Hit
TGTGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2160.40457772200265973No Hit
TGTGCGGGGGAAATATAGATCAATGGAACAAAATAGAAAGCCCAGAGATAAA2130.398958586974845No Hit
TGTGCGGGGTGTCAGAGCGGAACATTCTCAGCCGCCTGGCAAACAGGGCGCC2020.3783550918728577No Hit
TGTGCGGGGCACCAGAACACTGGCGAGAATGATAAGCTAAGATTTGAAATTT1990.372735956845043No Hit
TGTGCGGGGATGGCCAAGTGGAGTTCCCCCAGAGGGGGAGGTGGGGTAAGTG1950.3652437768079567No Hit
TGTGCGGGGCTCAGCCGTGTCCGACTCTTTGTGACCCCATGGACAACACAGT1950.3652437768079567No Hit
TGTGCGGGGCCCAGCACTCAGGAGGCAGAGGCAGGTGAATTTCTGAGTTCAA1880.3521324617430557No Hit
TGTGCGGAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1850.34651332671524093No Hit
TGTGCGGGGGGGGTTCAGGATGGGGAGCACATGTATACCTGTGATGATTTCA1810.3390211466781547No Hit
TGTGCGGGGGTCGGAACAGTGGCGTAATGGCGAGTTGATCTGGGCCGTGAGG1770.3315289666410684No Hit
TGTGCGGGGAGTTTGAAAAAGGAAATGAGGAGAAAAGTATTTGTACTGTATA1760.3296559216317968No Hit
TGTGCGGGGGTGAGTTCATCTCTATACCCATCTCTGATGAAGGTCTGAGTAG1710.32029069658543896No Hit
TGTGCGGGTACATCATACACAGAACTTAACTCAAACTGGATAAAAAAATTGA1620.30343329150199483No Hit
TGTGCGGGGGTGCTTGTGCGGGGTTGTGCGGGGGGGAAAAATTAGCTCATGA1620.30343329150199483No Hit
TGTGCGGGGGTGCAGTGCTTGTGCGGGGATGAATGTTCATTACAATAAAATG1580.2959411114649085No Hit
TGTGCGGGAGAACTTGTTTTTGTTGATAGTATTTGCTTTCTAGAACTTGTTT1560.29219502144636533No Hit
TGTGCGGGGATTAAAAGGCATGTGCCACCACCCCCAGCTTTTGTTGAAAATT1520.28470284140927904No Hit
TGTGCGGGGGGGGGGGAGGCCCCTCCCACCTCCAGTTTCCCTGACTTCTCAG1520.28470284140927904No Hit
TGTGCGGGGAAGGACAGTGCTGTGTGAAGGTGGAGTACCCCAGAGGCCGGTT1510.2828297964000075No Hit
TGTGCGGGAGCTCTGCCGGCCCGAGAGGGGCGGGCACTTTTTCAGCTTGCTA1470.2753376163629212No Hit
TGTGCGGGGGGGGGGAGGCCCCTCCCACCTCCAGTTTCCCTGACTTCTCAGA1440.2697184813351065No Hit
TGTGCGGGGTGACTCGTACCAGTTTGGAACTATTTACCCGGATCTGTAACAT1400.2622263012980202No Hit
TGTGCGGGGTGACCGGTTAGACCTAATTGATATCTGTAGGACATTTCACCCC1370.25660716627020547No Hit
TGTGCGGGGAAATCACTGTTGGCTCAAGAAAATTCTTTGGATTCTGATAAAT1370.25660716627020547No Hit
TGTGCGGGGGTGCAGTGCTTGTGCGGGGAAAAAAAAAAAAAAAAAAAAAAAA1280.2397497611867613No Hit
TGTGCGGGGGAAAAGATGTAAACAATAGAGCGCGCTCTGCGCTTTGGCGCGC1250.2341306261589466No Hit
TGTGCGGGGGAGTGCAGTGCTTGTGCGGGGAGACTGGGCTGGATCCAAACTT1190.22289235610331715No Hit
TGTGCGGGGCTCATCACATAATATCAATGAAAGTCCACATGTATGGATACAA1180.2210193110940456No Hit
TGTGCGGGTAAGGCGGCCCTGGCATTGGCTTGGTGATTACTGGCTGCACTCT1180.2210193110940456No Hit
TGTGCGGGTGAGACCTCACTCTTTGGGATCAAACACACATGGGCCGTGAGGC1160.21727322107550243No Hit
TGTGCGGGGAGGGCACTTTACAGGGGGTCGTGGTGGCTTCAAGATCCTCCAA1150.21540017606623088No Hit
TGTGCGGGGGAGTGCAGTGCTCTGTGTGGGGAGCCCTGGGCCCCACAACTGC1100.206034951019873No Hit
TGTGCGGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.20416190601060144No Hit
TGTGCGGGGAACAATAAACAAAAAGGAATGGCCTGGGTCTCTGTTTTATAAG1080.20228886100132987No Hit
TGTGCGGGGTTTCTTCCAATTATCTTCAGAATCTCAAAAAACTAATTTACTG1080.20228886100132987No Hit
TGTGCGGGGATGAATTAAATGAATTAACAATGTTCATAAACATGCCACCAGT1040.19479668096424357No Hit
TGTGCGGGGAAGGAAATGTATTTTGACACTGATATCTTATTAAAGTATTCTG1010.18917754593642885No Hit
TGTGCGGGGGCTTGTGCAAGCGGTCAGAGTTGCGTCATGGGACAGACGCGGG1010.18917754593642885No Hit
TGTGCGGGGAAAGAGGTGATTATCAAAAACATGTTTGAAATCCTGATGAAAA1010.18917754593642885No Hit
TGTGCGGGGATTTTAAAAAAATGAAAAATTAATTTAAAAAAATGCAAAAAAC1010.18917754593642885No Hit
TGTGCGGGGAATGTTGATGAATCAGCATATTCCTGCAGTAATAAAGAAATGC1000.18730450092715728No Hit
TGTGCGGGGAGATGGTACTCAGATAAAGCGAAACTAGCAACAGTTTCTGGAA980.18355841090861413No Hit
TGTGCGGGGTCCCTAGCCTCGCCTCACATCGGCCCAGCCTGGGCTGGATCCC980.18355841090861413No Hit
TGTGCGGGGTATTTGCTAGGATGTCACAATCTTTATTTTAGGTCTTCTCATT970.18168536589934256No Hit
TGTGCGGGGGATTGCCATCTGTCCTAGCTCTACCTGCCTCAAGTTGAAATTA970.18168536589934256No Hit
TGTGCGGGTTATCACGGGTGGATTCAGGCAACCCTACCCATCCTCACTGTCA960.179812320890071No Hit
TGTGCGGGGCTGACTGGCTTTTATTTTCCTTTTCAAAATCAGGTTTTTGTTC960.179812320890071No Hit
TGTGCGGGGAGATAGGAATGCACACATTTGCATACTTAGATAGAAACACATT940.17606623087152784No Hit
TGTGCGGGGAGCCTGAGTGTTAGGAAAACATCGCTCAGAACTATTGATTCAA930.17419318586225627No Hit
TGTGCGGGGAGGACTTCCCTGATAGCTCAGTTGGTGAAGAATCTGCCTGCAA920.1723201408529847No Hit
TGTGCGGGGAAGCACTGCTACTGTCCTCCATGACAACTTTTTTTTTTTGGTC920.1723201408529847No Hit
TGTGCGGGGAGGAGAAAAAGGAGGTAGTAAGGGAAAAAGGGCTCTCAGTGCC900.16857405083444155No Hit
TGTGCGGGGCACCTTAGCAAAGCATCAAGATCAGGTTTGATCCTCCACATTC890.16670100582516997No Hit
TGTGCGGGGGGTGCCATGCTCCATGCCTATAATCCCAGCATTGAGAGGCCAA880.1648279608158984No Hit
TGTGCGGGGATTCACCTGCGGCCAATGTTCCAGGCGATTCGCTGGCCGGTGG840.1573357807788121No Hit
TGTGCGGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA810.15171664575099741No Hit
TGTGCGGGGAGACGCAGGTTTGATCCCTGGGTTGGGAAGATCCCTGGAGAAG800.14984360074172584No Hit
TGTGCGGGGCAGAAGGCTTCTCCTCACAGGGCACTGTGTAACCATGAAAGCC790.14797055573245424No Hit
TGTGCGGGGGTGTGTGTGTGTTTTAAATGAGGTCTGCAACCAAAGGCAGGGC770.1442244657139111No Hit
TGTGCGGGGAAGTCCTAGAGTCCTGGGATCCTGGCCTGCTCCTTGCCAGCGG770.1442244657139111No Hit
TGTGCGGGGATCAGTTTACTATTTATTGTTGAGGGGGCTCAACTTTCTAATG760.14235142070463952No Hit
TGTGCGGGAGAGTCACCCAGCTCCAGGCCCCTGGCCACTTTGGTACTTGGTT760.14235142070463952No Hit
TGTGCGGGGGAGTGCAGTGCTTGTGCGGGGAAAAAAAAAAAAAAAAAAAAAA760.14235142070463952No Hit
TGTGCGGGGATGTTGAAGAAAGAATCTTGACTCCAGAACCAAAACCTTAGGG760.14235142070463952No Hit
TGTGCGGGTGTCCACCTCCTTTCCAAAGCCTCCTGCTTCCAGGTCTCAGTGC760.14235142070463952No Hit
TGTGCGGTTGCGGGGTGGTGGCTCACAACCATCCGTAGCGAGATCTGACTCC740.1386053306860964No Hit
TGTGCGGGGCAGGCATAGAGGCATAGATCCACATACCTATGGATATCCAATT730.13673228567682483No Hit
TGTGCGGGGGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.13298619565828168No Hit
TGTGCGGGGAGCCCATTGTCCTATTCTGATCTCCCCCGCAGGCCCAGGATCT710.13298619565828168No Hit
TGTGCGGGGCTGGGAACTTATGGATATATAACTCATACATCTCCTTTGTGTA710.13298619565828168No Hit
TGTGCGGGGGTGCAGTGCTTGTGCGGGGGAAAAAAAAAAAAAAAAAAAAAAA700.1311131506490101No Hit
TGTGCGGGGAACCAATCAAAATGAAGAATTGTGGAGCCCAGTTCCAACAGAC700.1311131506490101No Hit
TGTGCGGGGGTGCTTGTGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.1311131506490101No Hit
TGTGCGGGGGTATAATCCCAACCCTTGGGATGCTGAGGCAGGAGGACCGCTG700.1311131506490101No Hit
TGTGCGGGGCAGAGTGCAGTGCTTGTGCGGGGGCCTTCTCTAGGTAACCGTG690.1292401056397385No Hit
TGTGCGGGGAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.1292401056397385No Hit
TGTGCGGGTAGTCTGAGTTACCCATGTATCTTAAAAGTATTGAGACTTAGTG690.1292401056397385No Hit
TGTGCGGGGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA680.12736706063046693No Hit
TGTGCGGGGGTCCAAATGGCATTGGCCTGTGTTAGGAATCTCCATTTCTCGG680.12736706063046693No Hit
TGTGCGGGGGGTCGCTTCTGGCTCCTTCCCAGGTGGGCTAGCATCTGTGAGT680.12736706063046693No Hit
TGTGCGGGGAGTGCTTGTGCGGGGCTTTTCCCCTGCCGCCGCCGACTTGCGC680.12736706063046693No Hit
TGTGCGGGGCTTGTGCGGGGGTGCTTGTGCGGGAAGCAGTGGTATCAACGCA670.12549401562119536No Hit
TGTGCGGGGAGCCCCCTAAAAATAATGTCTGTCACTATTTCCATTGTTTCCC670.12549401562119536No Hit
TGTGCGGGGGAGAAGCTGATGAATCACTACAGTGTAAATAAAACCTTTTTCT670.12549401562119536No Hit
TGTGCGGGGAACTTAATGGAAGATTACAGTAACTCTAAATAAGCAAAACCAC660.1236209706119238No Hit
TGTGCGGGGGCAGTGCTTGTGCGGGGCTTGTGCGGGGAGTGGTATCAGTGCT650.12174792560265223No Hit
TGTGCGGGGGCAGTGCTTGTGCGGGGGGCAAAAAAAAAAAAAAAAAAAAAAA640.11987488059338065No Hit
TGTGCGGGGGTGCAGTGCTTGTGCGGGGGGAAAAAAAAAAAAAAAAAAAAAA640.11987488059338065No Hit
TGTGCGGGGATTCTTGCTTTTCAGCTGCTAATCACTGAGCCTTGGCTAGAGC630.1180018355841091No Hit
TGTGCGGGGAGAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.1180018355841091No Hit
TGTGCGGGGCAACAAGCATCATGGGCACAGACACGTCTATGAGGTGGAAGTG630.1180018355841091No Hit
TGTGCGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA620.11612879057483752Illumina Single End Adapter 2 (97% over 34bp)
TGTGCGGGGAGGAATAATACCTCCAAAAGGCTGTTTGCTCTATGGACCGCCA620.11612879057483752No Hit
TGTGCGGGGATTCACATATCCACATAAATATAGCCCCAACACACACACACAC610.11425574556556595No Hit
TGTGCGGGGACTTCAAAATAGGAAAAAGCCACCTCCCCATAGAAAGAACTGC610.11425574556556595No Hit
TGTGCGGGGATGGTGATCGTTGCTGTTCTGGGTGTCCTTGGAGCTATGGCCA590.1105096555470228No Hit
TGTGCGGGGAGTGCAGTGCTTGTGCGGGGAGCGAGTCTCATTCCTTCTCAGA590.1105096555470228No Hit
TGTGCGGGGTAGTGAATTAATAAAATAATTACCCCTCCATGAAGATTCATAC590.1105096555470228No Hit
TGTGCGGGGCATGTGGTTGCTGGGAATTGAACTCAGGACCTCTGGAAGAAGT580.10863661053775121No Hit
TGTGCGGGAGACCTAGCCTAGCTCAGCGCAGTTATTTTTGTGTTGCTTGGTT580.10863661053775121No Hit
TGTGCGGGGGTGAGTAAGCTTTGCAGTTCCTGTGAATTCGTGTCTCTTGGAG580.10863661053775121No Hit
TGTGCGGGGATTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.10676356552847964No Hit
TGTGCGGGTCAGTGAATACAGAAAGAGCATCTCGCCCTGGGAAGAAGTACAG570.10676356552847964No Hit
TGTGCGGGGCGGGGCTTGTGCGGAGGGAGATACCTTGGATGTCCAGCCCTAA570.10676356552847964No Hit
TGTGCGGGGGGTTCCCGACCTTCCGGTCGCACCTCAGGATGAGGCCGGTCTC570.10676356552847964No Hit
TGTGCGGGGGTGCAGTGCTTGTGCGGGAAAAAAAAAAAAAAAAAAAAAAAAA550.1030174755099365No Hit
TGTGCGGAGAGAGAAGGCCCTTCGAGATGCCAAGCTGGACAAGTCACAGATC550.1030174755099365No Hit
TGTGCGGGGCGCTGACAGTCAAAAGTCTCAAGGAAGAAAAGGAGGAATTCTT540.10114443050066493No Hit
TGTGCGGGAATGTGATTTCTCCATACTGCCAAGGCTTCCGTGTGTAACATCC540.10114443050066493No Hit
TGTGCGGGGTGTGCAGTTCTTGTGCGGGGATTTACTTTATATTTTTACTTAA540.10114443050066493No Hit
TGTGCGGGGCAGTGCCTGTGCGGGGGCGTGAGTTCAGTGCCTGTGCGGGGGC540.10114443050066493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATGAT206.2572234E-446.00000442
GAGAATG359.9526005E-846.00000425
TTTGAAT206.2572234E-446.00000436
AAACTTA405.4405973E-946.00000422
GAGGCAT206.2572234E-446.00000418
TCCTTGC206.2572234E-446.00000430
GAGAAAT206.2572234E-446.00000422
GAGAAAG206.2572234E-446.00000426
AAGTACT206.2572234E-446.00000430
GGTGAAT206.2572234E-446.00000434
TAAATTA405.4405973E-946.00000416
GGGTCCC206.2572234E-446.0000047
CATTGCT206.2572234E-446.00000441
GTGTCAG206.2572234E-446.0000049
GGGTTCA206.2572234E-446.00000411
TCCCAAG206.2572234E-446.00000410
CCTATGG206.2572234E-446.00000436
GGCACCA359.9526005E-846.0000048
GCCCAGA359.9526005E-846.00000440
AAATCTC206.2572234E-446.00000425
Produced by FastQC (version 0.11.5)