FastQCFastQC Report
Fri 17 Jun 2016
SRR1527531_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527531_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116386
Sequences flagged as poor quality0
Sequence length50
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAA53104.562404412901895No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAA16121.3850463114120255No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14201.2200780162562508No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCTGTGGGGA13911.195160930008764No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12791.0989294245012287No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAA8290.7122849827298816No Hit
GCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8200.7045520938944547No Hit
GCTGTGGGGAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAA7240.6220679463165674No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAAA6290.5404430086092828No Hit
GCTGTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5430.46655095973742544No Hit
GCTGTGGGGGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA4840.41585757737184886No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAG4720.40554705892461296No Hit
GCTGTGGGGACCTGTTCTGTCTGAGGCCGGCCCTTTATGAAGTACCAGAT3920.3368102692763734No Hit
GCTGTGGGGCAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAA3600.3093155534170776No Hit
GCTGTGGGGGATAAAAGTGGGTGTGGCATCTCACAAAGACAGCCACAAGG3450.29642740535803275No Hit
GCTGTGGGGATTGCCATTATGGAGATTTAAAACACCTCTAATTTTAAATA3390.29127214613441477No Hit
GCTGTGGGGGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAA3370.2895537263932088No Hit
GCTGTGGGGGTAAACTTATACTTTTCATCACACTCAGAAGATGTTGCCAA3140.26979189936933995No Hit
GCTGTGGGGGTGGTGATCCAGTGGTTAGGACTCTACCCTTCCACTTCCAG2790.23971955389823518No Hit
GCTGTGGGGTGGTGGACAGGCAGTAAATGCGCTGCTTGGGGACTTCTCTG2770.2380011341570292No Hit
GCTGTGGGGCGTTATAAAAATCCTTCGACTGGCGCATGTACGTTTGAAGG2650.22769061570979326No Hit
GCTGTGGGGGAATGTGAAATAATCTCAAGGATTCTCCACCAAATGGAACT2650.22769061570979326No Hit
GCTGTGGGGTGCAACAAGAGAAGCCACGGCAATGAGAAGCCTGCACACTG2640.2268314058391903No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAA2640.2268314058391903No Hit
GCTGTGGGGAGTTGTCCAACAGCTTGTTGGTTAAGTGTTAAGAGTGGTGG2530.21738009726255733No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGCAAAAAAAAAAAAAAAAAAA2480.21308404790954236No Hit
GCTGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCAC2460.2113656281683364No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGGAAAAAAAAAAAAAAAAAAA2390.20535115907411544No Hit
GCTGTGGGGGAGTGCAATGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAA2350.20191431959170345No Hit
GCTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT2300.19761827023868853No Hit
GCTGTGGGGGCTGCTGTGGGGAAACCAAAATCTGTCTGTCTACTGTTTAT2180.1873077517914526No Hit
GCTGTGGGGATGGACTCAAGGATGAGTCCCAGTTTACTGACCTAAATGCC2070.17785644321481967No Hit
GCTGTGGAGAGAGACCCTCTTAAGCCCCTTGCTTCTCAGTGGGGTAGGCA2060.17699723334421666No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGGAGTGCAGTGCTGCTGTGGG2040.17527881360301067No Hit
GCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2030.17441960373240767No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCG1920.16496829515577474Illumina Single End Adapter 2 (100% over 23bp)
GCTGTGGGGAAGAGCCCCTGTGGCCAATTGAAAAGCCTCTGAGGGTTTAG1910.16410908528517176No Hit
GCTGTGGGGGCTGCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAA1910.16410908528517176No Hit
GCTGTGGGGGTTTTTAGGTCCAGAGCTTTTGGAAATTTAGAAGTCTTATT1850.1589538260615538No Hit
GCTGTGGGAAGTAATTAAGAGGTCAGCCATGTGACAGCTGCCCAACCAAG1790.15379856683793583No Hit
GCTGTGGGGGGTCTTTAGCCATGCACAAACGGTAGTCTTGTGTGTTGGCT1790.15379856683793583No Hit
GCTGTGGGAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAA1750.15036172735552386No Hit
GCTGTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1690.1452064681319059Illumina Single End Adapter 2 (97% over 34bp)
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAAAA1680.1443472582613029No Hit
GCTGTGGGGGCTGCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAA1680.1443472582613029No Hit
GCTGTGGGGATCCTATTGTTAGTCAGTCTGGTAGCCTTGAATTACATTGC1640.14091041877889093No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGCAAAAAAAAAAAAAAAAAAAA1630.14005120890828793No Hit
GCTGTGGGGGCAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAA1600.13747357929647896No Hit
GCTGTGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAA1600.13747357929647896Illumina Single End Adapter 2 (100% over 34bp)
GCTGTGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA1560.13403673981406697Illumina Single End Adapter 2 (97% over 34bp)
GCTGTGGGGATGGAGAGACTGGGCAGTCTTGCCTTTTCCCTGATTTTATT1520.130599900331655No Hit
GCTGTGGGGGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA1500.12888148059044902No Hit
GCTGTGGGGAGAAGAGAAAAAAAAATAAAACAGAAACTACAGAACTAGTT1490.12802227071984604No Hit
GCTGTGGGGGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1490.12802227071984604No Hit
GCTGTGGGGCAGTGCTGCTGTGGGGGTGCAGTGCTGCTGTGGGGAGTGCA1420.12200780162562508No Hit
GCTGTGGGGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA1420.12200780162562508No Hit
GCTGTGGGTGAGCTGGAGGCGGAGGCGGCTGGGGAGGTCCGAGCGATGTG1420.12200780162562508No Hit
GCTGTGGGGATCTTTAAAATATACTTCATTTTTTCATGTGCTCTGTCCTC1410.12114859175502209No Hit
GCTGTGGGGGGAGAACCACTTGAACCCAGGAGGCGGAGGTTGCGGTGAGC1410.12114859175502209No Hit
GCTGTGGGGAGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAA1370.1177117522726101No Hit
GCTGTGGGGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAA1350.11599333253140412No Hit
GCTGTGGGGTCCATTTTGTGTTGGGAGATCGTCGTAATCGTTTGCAGACT1320.11341570291959514No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAGG1290.11083807330778617No Hit
GCTGTGGGGATGAAAGGTCTTGGGGAACTATCACATGGTCTGAAAGGAAG1260.10826044369597718No Hit
GCTGTGATTTCTTTTTTTTTAATGATTCCAAATAAAACCTGAGCCCCACT1260.10826044369597718No Hit
GCTGTGGGGATCATGACTTCTCGTCACTGGAGGAACCTGCCTGCACCACA1250.1074012338253742No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAGATCGGAAGAGCTCGTATGCCGTCT1220.10482360421356521Illumina Single End Adapter 2 (100% over 26bp)
GCTGTGGGATTTTCCAGGCAAGAACACTGGAGTGGGTTGCCATTTCCTTC1200.10310518447235921No Hit
GCTGTGGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.10310518447235921No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGGA351.4311263E-744.017
CAGCCAT302.5080772E-644.024
CAGCCAC351.4311263E-744.040
CTTTTCA254.4181397E-544.021
CGCTGAT207.8273093E-444.040
AAACTTA254.4181397E-544.012
GAGGCAT254.4181397E-544.09
GAGGTCA302.5080772E-644.019
TGTTGCG207.8273093E-444.012
TTGTTGC207.8273093E-444.011
GGATGGA254.4181397E-544.08
CAAGAGA254.4181397E-544.015
CCAATTG207.8273093E-444.024
GGGTCTT254.4181397E-544.09
AATCCTT454.7111826E-1044.019
TTCATGT302.5080772E-644.033
CAAGAAC207.8273093E-444.019
TCCTTCG454.7111826E-1044.021
GCCTTTG207.8273093E-444.018
TTCATCA254.4181397E-544.024
Produced by FastQC (version 0.11.5)