SRR1527530_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527530_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	102129
Sequences flagged as poor quality	0
Sequence length	52
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAA	4638	4.541315395235437	No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAA	1444	1.4138981092539826	No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1261	1.2347129610590528	No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCTGTGGGGAAG	898	0.8792801261150115	No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAA	763	0.7470943610531778	No Hit
GCTGTGGGGAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAA	701	0.6863868245062618	No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	689	0.6746369787229877	No Hit
GCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	654	0.6403665951884382	No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAAAAA	572	0.5600759823360651	No Hit
GCTGTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	506	0.49545183052805764	No Hit
GCTGTGGGGACCTGTTCTGTCTGAGGCCGGCCCTTTATGAAGTACCAGATGG	363	0.3554328349440414	No Hit
GCTGTGGGGCAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAA	335	0.3280165281164018	No Hit
GCTGTGGGGATTGCCATTATGGAGATTTAAAACACCTCTAATTTTAAATACC	314	0.30745429799567214	No Hit
GCTGTGGGGGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAA	310	0.3035376827345808	No Hit
GCTGTGGGGGATAAAAGTGGGTGTGGCATCTCACAAAGACAGCCACAAGGAG	280	0.2741630682763955	No Hit
GCTGTGGGGGTGGTGATCCAGTGGTTAGGACTCTACCCTTCCACTTCCAGGG	279	0.27318391446112267	No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG	270	0.2643715301236671	No Hit
GCTGTGGGGGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	266	0.26045491486257577	No Hit
GCTGTGGGGGTAAACTTATACTTTTCATCACACTCAGAAGATGTTGCCAATA	261	0.2555591457862116	No Hit
GCTGTGGGGTGGTGGACAGGCAGTAAATGCGCTGCTTGGGGACTTCTCTGGT	251	0.24576760763348315	No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG	248	0.24283014618766463	No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGGAAAAAAAAAAAAAAAAAAAAA	240	0.2349969156654819	No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAA	238	0.2330386080349362	No Hit
GCTGTGGGGAGTTGTCCAACAGCTTGTTGGTTAAGTGTTAAGAGTGGTGGTG	233	0.228142838958572	No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGCAAAAAAAAAAAAAAAAAAAAA	228	0.22324706988220777	No Hit
GCTGTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA	221	0.21639299317529792	Illumina Single End Adapter 2 (97% over 34bp)
GCTGTGGGGATGGACTCAAGGATGAGTCCCAGTTTACTGACCTAAATGCCTG	218	0.2134555317294794	No Hit
GCTGTGGGGGCTGCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAA	216	0.2114972240989337	No Hit
GCTGTGGGGGAATGTGAAATAATCTCAAGGATTCTCCACCAAATGGAACTGA	213	0.20855976265311518	No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGG	210	0.20562230120729666	No Hit
GCTGTGGGGCGTTATAAAAATCCTTCGACTGGCGCATGTACGTTTGAAGGCA	210	0.20562230120729666	No Hit
GCTGTGGGGGAGTGCAATGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAA	205	0.20072653213093244	No Hit
GCTGTGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	199	0.1948516092392954	Illumina Single End Adapter 2 (100% over 34bp)
GCTGTGGGGTGCAACAAGAGAAGCCACGGCAATGAGAAGCCTGCACACTGCA	198	0.19387245542402254	No Hit
GCTGTGGAGAGAGACCCTCTTAAGCCCCTTGCTTCTCAGTGGGGTAGGCAAA	182	0.1782059943796571	No Hit
GCTGTGGGGGCTGCTGTGGGGAAACCAAAATCTGTCTGTCTACTGTTTATTA	181	0.17722684056438426	No Hit
GCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	178	0.17428937911856573	No Hit
GCTGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG	174	0.17037276385747438	No Hit
GCTGTGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA	172	0.1684144562269287	Illumina Single End Adapter 2 (97% over 34bp)
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGG	172	0.1684144562269287	No Hit
GCTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	171	0.16743530241165586	No Hit
GCTGTGGGGGTTTTTAGGTCCAGAGCTTTTGGAAATTTAGAAGTCTTATTTC	165	0.1615603795200188	No Hit
GCTGTGGGGGCTGCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAA	164	0.16058122570474595	No Hit
GCTGTGGGAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAA	163	0.15960207188947312	No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAA	161	0.15764376425892743	No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCTGTGGGGGAG	153	0.14981053373674472	No Hit
GCTGTGGGGGGTCTTTAGCCATGCACAAACGGTAGTCTTGTGTGTTGGCTGC	152	0.14883137992147188	No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC	147	0.14393561084510767	Illumina Single End Adapter 2 (100% over 25bp)
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGGAGTGCAGTGCTGCTGTGGGGA	144	0.14099814939928915	No Hit
GCTGTGGGTGAGCTGGAGGCGGAGGCGGCTGGGGAGGTCCGAGCGATGTGAC	143	0.1400189955840163	No Hit
GCTGTGGGGGGAGAACCACTTGAACCCAGGAGGCGGAGGTTGCGGTGAGCCA	141	0.1380606879534706	No Hit
GCTGTGGGGTCCATTTTGTGTTGGGAGATCGTCGTAATCGTTTGCAGACTTC	140	0.13708153413819776	No Hit
GCTGTGGGGAGAAGAGAAAAAAAAATAAAACAGAAACTACAGAACTAGTTAT	139	0.13610238032292493	No Hit
GCTGTGGGGGCAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAA	137	0.13414407269237924	No Hit
GCTGTGGGAAGTAATTAAGAGGTCAGCCATGTGACAGCTGCCCAACCAAGTC	137	0.13414407269237924	No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAG	127	0.12435253453965084	No Hit
GCTGTGGGGAAGAGCCCCTGTGGCCAATTGAAAAGCCTCTGAGGGTTTAGAC	125	0.12239422690910515	No Hit
GCTGTGGGATTTTCCAGGCAAGAACACTGGAGTGGGTTGCCATTTCCTTCTC	125	0.12239422690910515	No Hit
GCTGTGGGGATGGAGAGACTGGGCAGTCTTGCCTTTTCCCTGATTTTATTGT	123	0.12043591927855947	No Hit
GCTGTGGGGAGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAA	119	0.1165193040174681	No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGCAAAAAAAAAAAAAAAAAAAAAA	118	0.11554015020219525	No Hit
GCTGTGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA	116	0.11358184257164958	Illumina Single End Adapter 2 (97% over 34bp)
GCTGTGGGGATCCTATTGTTAGTCAGTCTGGTAGCCTTGAATTACATTGCCA	114	0.11162353494110389	No Hit
GCTGTGGGGATCTTTAAAATATACTTCATTTTTTCATGTGCTCTGTCCTCAG	112	0.10966522731055822	No Hit
GCTGTGGGGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA	112	0.10966522731055822	No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGG	109	0.1067277658647397	No Hit
GCTGTGGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	107	0.10476945823419401	No Hit
GCTGTGGGGAAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAA	107	0.10476945823419401	Illumina Single End Adapter 2 (100% over 34bp)
GCTGTGGGGATGAAAGGTCTTGGGGAACTATCACATGGTCTGAAAGGAAGGG	103	0.10085284297310264	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCCAT	35	1.0073745E-7	46.000004	24
GATGGAC	35	1.0073745E-7	46.000004	9
GAGGTCA	35	1.0073745E-7	46.000004	19
CAGTTTA	35	1.0073745E-7	46.000004	30
CCCAGTT	35	1.0073745E-7	46.000004	28
TCCCAGT	35	1.0073745E-7	46.000004	27
CCAACCA	35	1.0073745E-7	46.000004	42
GCCCAAC	35	1.0073745E-7	46.000004	40
TGACCTA	35	1.0073745E-7	46.000004	38
CCAGTTT	35	1.0073745E-7	46.000004	29
ACTGACC	35	1.0073745E-7	46.000004	36
GACAGCT	35	1.0073745E-7	46.000004	33
AGGATGA	35	1.0073745E-7	46.000004	19
CCTGCAC	35	1.0073745E-7	46.000004	40
CAACCAA	35	1.0073745E-7	46.000004	43
GAAGTAA	35	1.0073745E-7	46.000004	8
CCATGTG	35	1.0073745E-7	46.000004	27
TGACAGC	35	1.0073745E-7	46.000004	32
AATTAAG	35	1.0073745E-7	46.000004	13
ACCAAGT	35	1.0073745E-7	46.000004	45

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content