FastQCFastQC Report
Fri 17 Jun 2016
SRR1527529_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527529_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105534
Sequences flagged as poor quality0
Sequence length52
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAA46644.419428809672713No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAA14111.3370098735952396No Hit
GCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13941.320901320901321No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCTGTGGGGAAG8230.779843462770292No Hit
GCTGTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7950.753311728921485No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAA7660.7258324331495063No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7310.6926677658384975No Hit
GCTGTGGGGAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAA6650.6301286789091668No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAAAAA5320.5041029431273334No Hit
GCTGTGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4890.4633577804309512No Hit
GCTGTGGGGACCTGTTCTGTCTGAGGCCGGCCCTTTATGAAGTACCAGATGG4360.4131369985028522No Hit
GCTGTGGGGGATAAAAGTGGGTGTGGCATCTCACAAAGACAGCCACAAGGAG3570.3382796065722895No Hit
GCTGTGGGGCAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAA3410.3231186158015426No Hit
GCTGTGGGGGTAAACTTATACTTTTCATCACACTCAGAAGATGTTGCCAATA3290.3117478727234825No Hit
GCTGTGGGGGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAA3280.3108003108003108No Hit
GCTGTGGGGATTGCCATTATGGAGATTTAAAACACCTCTAATTTTAAATACC3250.30795762503079577No Hit
GCTGTGGGGGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3000.28426857695150376No Hit
GCTGTGGGGGAATGTGAAATAATCTCAAGGATTCTCCACCAAATGGAACTGA2890.2738453957966153No Hit
GCTGTGGGGGTGGTGATCCAGTGGTTAGGACTCTACCCTTCCACTTCCAGGG2860.27100271002710025No Hit
GCTGTGGGGCGTTATAAAAATCCTTCGACTGGCGCATGTACGTTTGAAGGCA2780.2634222146417268No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG2720.25773684310269673No Hit
GCTGTGGGGAGTTGTCCAACAGCTTGTTGGTTAAGTGTTAAGAGTGGTGGTG2630.24920878579415165No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTGCTGTGGGGGAG2580.24447097617829325No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGGAAAAAAAAAAAAAAAAAAAAA2530.23973316656243485No Hit
GCTGTGGGGTGCAACAAGAGAAGCCACGGCAATGAGAAGCCTGCACACTGCA2390.22646729963803133No Hit
GCTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT2280.21604411848314287No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGCAAAAAAAAAAAAAAAAAAAAA2270.2150965565599712No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAA2260.2141489946367995No Hit
GCTGTGGGGATGGACTCAAGGATGAGTCCCAGTTTACTGACCTAAATGCCTG2260.2141489946367995No Hit
GCTGTGGGGGAGTGCAATGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAA2240.21225387079045616No Hit
GCTGTGGGGGCTGCTGTGGGGAAACCAAAATCTGTCTGTCTACTGTTTATTA2180.2065684992514261No Hit
GCTGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAG2090.19804044194288098No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGG1930.1828794511721341No Hit
GCTGTGGGGGGTCTTTAGCCATGCACAAACGGTAGTCTTGTGTGTTGGCTGC1930.1828794511721341No Hit
GCTGTGGGGGTTTTTAGGTCCAGAGCTTTTGGAAATTTAGAAGTCTTATTTC1840.174351393863589No Hit
GCTGTGGGGGCTGCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAA1840.174351393863589No Hit
GCTGTGGAGAGAGACCCTCTTAAGCCCCTTGCTTCTCAGTGGGGTAGGCAAA1780.1686660223245589No Hit
GCTGTGGGGAGTGCAGTGCTGCTGTGGGGTAAAAAAAAAAAAAAAAAAAAAA1720.16298065078552884No Hit
GCTGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1710.16203308886235715No Hit
GCTGTGGGAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAA1680.1591904030928421No Hit
GCTGTGGGGAAGCAGTGGTATCAACGCAGATCGGAAGAGCTCGTATGCCGTC1640.1554001554001554Illumina Single End Adapter 2 (100% over 25bp)
GCTGTGGGATTTTCCAGGCAAGAACACTGGAGTGGGTTGCCATTTCCTTCTC1640.1554001554001554No Hit
GCTGTGGGAAGTAATTAAGAGGTCAGCCATGTGACAGCTGCCCAACCAAGTC1610.15255746963064037No Hit
GCTGTGGGGGCTGCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAA1610.15255746963064037No Hit
GCTGTGGGGTCCATTTTGTGTTGGGAGATCGTCGTAATCGTTTGCAGACTTC1540.14592453616843862No Hit
GCTGTGGGGGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGG1520.14402941232209526No Hit
GCTGTGGGGATCCTATTGTTAGTCAGTCTGGTAGCCTTGAATTACATTGCCA1500.14213428847575188No Hit
GCTGTGGGGATGGAGAGACTGGGCAGTCTTGCCTTTTCCCTGATTTTATTGT1480.14023916462940852No Hit
GCTGTGGGGGGAGAACCACTTGAACCCAGGAGGCGGAGGTTGCGGTGAGCCA1450.1373964788598935No Hit
GCTGTGGGTGAGCTGGAGGCGGAGGCGGCTGGGGAGGTCCGAGCGATGTGAC1440.13644891693672181No Hit
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGGAGTGCAGTGCTGCTGTGGGGA1440.13644891693672181No Hit
GCTGTGGGGATCTTTAAAATATACTTCATTTTTTCATGTGCTCTGTCCTCAG1390.1317111073208634No Hit
GCTGTGGGGAAGAGCCCCTGTGGCCAATTGAAAAGCCTCTGAGGGTTTAGAC1380.13076354539769175No Hit
GCTGTGGGGTGGTGGACAGGCAGTAAATGCGCTGCTTGGGGACTTCTCTGGT1340.126973297705005No Hit
GCTGTGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1290.12223548808914662Illumina Single End Adapter 2 (97% over 34bp)
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGCAAAAAAAAAAAAAAAAAAAAAA1270.12034036424280327No Hit
GCTGTGGGGAGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1260.11939280231963159Illumina Single End Adapter 2 (100% over 34bp)
GCTGTGGGGTAAGTTAGGTTTTTATATAGTGAAGGTAGTAGATAATCTTTTC1240.11749767847328824No Hit
GCTGTGGGGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAA1230.11655011655011654No Hit
GCTGTGGGGGCAGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAA1220.11560255462694487No Hit
GCTGTGGGGATGAAAGGTCTTGGGGAACTATCACATGGTCTGAAAGGAAGGG1190.11275986885742983No Hit
GCTGTGGGGAGAGTGCAGTGCTGCTGTGGGAAAAAAAAAAAAAAAAAAAAAA1180.11181230693425816No Hit
GCTGTGGGGAGAAGAGAAAAAAAAATAAAACAGAAACTACAGAACTAGTTAT1170.11086474501108648No Hit
GCTGTGGGGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1130.10707449731839976Illumina Single End Adapter 2 (97% over 34bp)
GCTGTGGGGGAGTGCAGTGCTGCTGTGGGGAAAAAAAAAAAAAAAAAAAAAG1110.1051793734720564No Hit
GCTGTGGGGAGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1070.10138912577936968No Hit
GCTGTGGGGATCATGACTTCTCGTCACTGGAGGAACCTGCCTGCACCACACT1060.100441563856198No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCCAT206.285295E-446.00000424
CAGCCAC206.285295E-446.00000440
CTCTGGT206.285295E-446.00000446
TAGAAAG206.285295E-446.00000421
GAGGCAT206.285295E-446.0000049
GAGGTCC206.285295E-446.00000434
GTAGAGC206.285295E-446.00000439
TTGCAGA206.285295E-446.00000442
CAATGAG405.527909E-946.00000430
GAGCGCA206.285295E-446.00000410
GGCATTC206.285295E-446.00000411
CCAACCA206.285295E-446.00000442
GGCATCT206.285295E-446.00000425
ATCTCAC206.285295E-446.00000428
GGGCAAC206.285295E-446.0000047
AGCCACG405.527909E-946.00000422
GACAGTG206.285295E-446.00000412
GGACTTT206.285295E-446.0000048
TGACTAG206.285295E-446.00000414
AAACAGT206.285295E-446.00000410
Produced by FastQC (version 0.11.5)