FastQCFastQC Report
Fri 17 Jun 2016
SRR1527525_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527525_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047805
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG68260.6514570936386064No Hit
TGAAGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC59160.5646088728341628No Hit
TGAAGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC50580.4827234075042589No Hit
TGAAGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA48020.4582913805526792No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC46490.4436894269448991No Hit
TGAAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC41690.3978793764106871No Hit
TGAAGCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG40880.39014893038303883No Hit
TGAAGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA38210.3646670897733834No Hit
TGAAGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG37830.3610404607727583No Hit
TGAAGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT35710.34080768845348136No Hit
TGAAGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA31870.30415964802611173No Hit
TGAAGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG30920.29509307552454894No Hit
TGAAGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA27710.2644576042297947No Hit
TGAAGCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA27600.263407790571719No Hit
TGAAGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC24520.2340130081455996No Hit
TGAAGCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC21550.20566803937755596No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG21160.2019459727716512No Hit
TGAAGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC21100.2013733471399736No Hit
TGAAGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG20920.19965547024494062No Hit
TGAAGCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC20460.19526534040207863No Hit
TGAAGCGGGAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTCGA20390.19459727716512137No Hit
TGAAGCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG17650.1684473733185087No Hit
TGAAGCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT16710.15947623842222552No Hit
TGAAGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT15690.14974160268370545No Hit
TGAAGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTT13750.13122670725946145No Hit
TGAAGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC13730.13103583204890223No Hit
TGAAGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC13550.12931795515386926No Hit
TGAAGCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA13230.12626395178492184No Hit
TGAAGCGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC12910.12320994841597435No Hit
TGAAGCGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGT12680.12101488349454337No Hit
TGAAGCGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCT12150.11595669041472412No Hit
TGAAGCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC11840.11299812465105626No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11690.11156656057186214No Hit
TGAAGCGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAA11500.10975324607154957No Hit
TGAAGCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT11480.10956237086099035No Hit
TGAAGCGGGGGCAGCGTGTCTCAGTCGGTTGTCGAGTCCCTTCTGTCCCA11100.10593574186036524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG600.044.033
CTATGCG207.8561634E-444.018
TCGTTTG207.8561634E-444.019
CAAACTA254.44257E-544.024
GGTACGA207.8561634E-444.044
ACACGTT254.44257E-544.017
CTCGTAA351.4459147E-744.018
CAAAACG207.8561634E-444.024
ATTACCA302.5275021E-644.033
ACCGATA254.44257E-544.027
TAAACGT207.8561634E-444.036
TGTATCG408.310963E-944.011
CACGATT207.8561634E-444.029
GATCACG207.8561634E-444.040
TAACGTG207.8561634E-444.030
TATGCGT207.8561634E-444.025
ATCACGT207.8561634E-444.041
GTAATAC302.5275021E-644.022
ATACTAC254.44257E-544.042
TATTGCG207.8561634E-444.018
Produced by FastQC (version 0.11.5)