##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527525_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1047805
Sequences flagged as poor quality	0
Sequence length	50
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8807230352976	33.0	31.0	34.0	30.0	34.0
2	32.0445082815982	34.0	31.0	34.0	30.0	34.0
3	32.61352446304417	34.0	31.0	34.0	31.0	34.0
4	36.08810227093782	37.0	35.0	37.0	35.0	37.0
5	36.04864931929128	37.0	35.0	37.0	35.0	37.0
6	36.20442544175682	37.0	37.0	37.0	35.0	37.0
7	36.477720568235505	37.0	37.0	37.0	35.0	37.0
8	36.64388221090756	37.0	37.0	37.0	35.0	37.0
9	38.63607255166753	39.0	39.0	39.0	38.0	39.0
10	37.29332175357056	39.0	37.0	39.0	34.0	39.0
11	36.86526118886625	38.0	37.0	39.0	32.0	39.0
12	36.50337705966282	38.0	35.0	39.0	32.0	39.0
13	36.673674013771645	39.0	37.0	39.0	32.0	39.0
14	37.49567047303649	39.0	37.0	40.0	33.0	40.0
15	37.58142402450838	40.0	37.0	40.0	33.0	40.0
16	37.713324521261114	40.0	37.0	40.0	33.0	40.0
17	37.63784101049337	39.0	37.0	40.0	33.0	40.0
18	37.46078325642653	39.0	37.0	40.0	33.0	40.0
19	37.37072260582838	39.0	36.0	40.0	33.0	40.0
20	37.13384265202018	39.0	36.0	40.0	33.0	40.0
21	37.02014401534637	38.0	36.0	40.0	33.0	40.0
22	36.743710900406086	38.0	35.0	40.0	32.0	40.0
23	36.46552459665682	38.0	35.0	40.0	31.0	40.0
24	36.26876279460396	38.0	35.0	40.0	31.0	40.0
25	36.00544757850936	38.0	35.0	39.0	31.0	40.0
26	35.33549276821546	37.0	34.0	39.0	30.0	40.0
27	35.07726819398648	37.0	34.0	39.0	29.0	40.0
28	34.84453214099952	36.0	33.0	38.0	29.0	40.0
29	34.467638539613766	36.0	33.0	38.0	28.0	40.0
30	34.135614928350215	35.0	33.0	38.0	28.0	39.0
31	33.948758595349325	35.0	33.0	38.0	28.0	39.0
32	33.76921564604101	35.0	32.0	38.0	27.0	39.0
33	33.20898163303286	34.0	32.0	37.0	26.0	39.0
34	32.76653194058055	34.0	31.0	37.0	26.0	38.0
35	32.50108369400795	34.0	31.0	36.0	25.0	38.0
36	32.14058627320923	34.0	30.0	36.0	24.0	38.0
37	31.868832464055814	33.0	30.0	36.0	24.0	38.0
38	31.374493345612972	33.0	30.0	35.0	23.0	38.0
39	30.442565171954705	32.0	29.0	35.0	21.0	37.0
40	30.14906113255806	32.0	28.0	34.0	21.0	37.0
41	29.77068729391442	31.0	27.0	34.0	21.0	36.0
42	29.507096263140564	31.0	27.0	34.0	21.0	36.0
43	29.059945314252175	31.0	26.0	34.0	20.0	36.0
44	28.66117550498423	31.0	26.0	33.0	19.0	35.0
45	28.2480929180525	30.0	25.0	33.0	20.0	35.0
46	28.046335911739302	30.0	25.0	33.0	19.0	34.0
47	27.548144931547377	29.0	24.0	32.0	18.0	34.0
48	26.984320555828614	29.0	23.0	32.0	18.0	34.0
49	26.388097021869527	29.0	23.0	31.0	17.0	34.0
50	29.753524749356988	31.0	27.0	34.0	22.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	6.0
13	4.0
14	21.0
15	61.0
16	124.0
17	253.0
18	473.0
19	813.0
20	1286.0
21	2149.0
22	3267.0
23	4605.0
24	6501.0
25	8727.0
26	11977.0
27	15800.0
28	21357.0
29	29086.0
30	39046.0
31	55008.0
32	87194.0
33	133724.0
34	185138.0
35	239797.0
36	174454.0
37	26456.0
38	476.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.34892465678251	0.571384942809015	0.031112659321152314	0.04857774108732064
8	99.72676213608447	0.1799953235573413	0.0688105134065976	0.024432026951579727
9	98.70615238522436	0.7823020504769493	0.2571089086232648	0.25443665567543583
10	63.52632407747625	27.83590458148224	3.0088613816502114	5.628909959391299
11	36.59096873941239	25.78390063036538	18.436254837493617	19.18887579272861
12	29.735208364151728	16.591636802649347	26.383248791521325	27.289906041677604
13	25.068691216399998	16.5885827992804	30.23024322273706	28.112482761582548
14	25.53118185158498	17.55612924160507	29.52572282056299	27.386966086246968
15	26.038814474067216	17.12761439389963	27.886963700306833	28.946607431726324
16	30.74589260406278	17.8068438306746	23.41905220914197	28.028211356120654
17	31.777573117135343	18.634287868448805	22.39968314715047	27.188455867265375
18	29.918257691078015	18.614818596971766	22.745930779104892	28.72099293284533
19	31.039649553113414	17.869641774948583	23.00943400728189	28.081274664656114
20	29.657331278243564	20.08312615419854	22.690863280858554	27.56867928669934
21	33.04727501777525	18.265994149674796	23.28982969159338	25.396901140956572
22	32.025901766072884	18.183536058713216	22.599815805421812	27.19074636979209
23	30.57000109753246	20.447793243971923	21.953226029652466	27.028979628843153
24	29.375313154642324	20.014315640791942	21.883747453008908	28.726623751556822
25	29.468746570211064	20.188584708032504	22.635795782612224	27.706872939144212
26	29.113241490544517	21.001999417830607	21.453132977987316	28.43162611363756
27	30.327685017727536	18.69345918372216	21.248228439451996	29.730627359098307
28	31.302484718053453	19.734110831691012	21.020037125228455	27.94336732502708
29	31.224703069750575	20.108798870018756	19.75634779372116	28.91015026650951
30	30.563988528399843	19.66129193886267	21.428987263851575	28.34573226888591
31	32.81288025920854	19.738214648718035	19.867246291056066	27.581658801017365
32	33.53038017570063	18.473380065947385	21.278386722720352	26.717853035631627
33	33.544218628466176	19.205195623231422	21.35206455399621	25.89852119430619
34	31.924165278844825	21.625779605938128	22.71224130444119	23.737813810775858
35	30.55826227208307	23.745448819198227	23.0552440578161	22.6410448509026
36	32.69825969526773	21.995218575975493	20.586177771627355	24.72034395712943
37	30.98878130949938	23.01420588754587	19.764364552564647	26.2326482503901
38	33.85591784730938	21.284876479879365	20.05907587766808	24.80012979514318
39	31.87291528480967	20.39702043796317	20.714255037912586	27.015809239314564
40	31.179656520058597	21.419634378534173	20.504864931929127	26.8958441694781
41	29.11114186322837	20.439776485128437	21.619957912016073	28.82912373962713
42	27.60914483133789	18.665877715796356	24.499310463301853	29.225666989563898
43	28.687876083813308	17.49533548704196	26.097413163708893	27.719375265435843
44	28.516279269520567	18.18678093729272	24.173486478877273	29.12345331430944
45	28.903660509350498	18.667404717480828	24.95569309174894	27.47324168141973
46	29.222040360563273	20.19058889774338	23.187616016338918	27.39975472535443
47	29.174703308344586	20.412863080439585	24.076044683886792	26.336388927329036
48	29.16334623331631	20.69030019898741	23.466484698965935	26.679868868730345
49	29.246090637093737	20.84280949222422	22.927643979557267	26.98345589112478
50	27.264996826699623	21.073291308974476	23.505327804314735	28.156384060011163
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	10.0
8	19.0
9	13.0
10	7.0
11	39.5
12	72.0
13	160.0
14	248.0
15	332.0
16	416.0
17	394.5
18	373.0
19	544.0
20	715.0
21	767.5
22	820.0
23	992.5
24	1165.0
25	1344.0
26	1523.0
27	1749.0
28	1975.0
29	2272.0
30	2569.0
31	2993.0
32	3417.0
33	4310.5
34	5204.0
35	6366.0
36	7528.0
37	8299.0
38	9070.0
39	10405.5
40	11741.0
41	14086.5
42	16432.0
43	18352.5
44	20273.0
45	21792.0
46	23311.0
47	28293.0
48	33275.0
49	35108.0
50	36941.0
51	42140.5
52	47340.0
53	50034.5
54	52729.0
55	65662.5
56	78596.0
57	84893.0
58	91190.0
59	100185.5
60	109181.0
61	109902.0
62	110623.0
63	107131.0
64	103639.0
65	95671.0
66	87703.0
67	74296.0
68	60889.0
69	51017.5
70	41146.0
71	35306.5
72	29467.0
73	24580.5
74	19694.0
75	22189.0
76	24684.0
77	16740.5
78	8797.0
79	5728.0
80	2659.0
81	2266.0
82	1873.0
83	1125.5
84	378.0
85	244.0
86	110.0
87	60.5
88	11.0
89	6.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1047805.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	10.771661875731684
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.87511635163844	7.6344278874299105
2	9.86047664901616	2.124274407934998
3	3.692617186049782	1.1932687129393216
4	1.7197238691937733	0.740971361543214
5	0.8625918228107586	0.4645773726044274
6	0.5492084098882017	0.3549532374374378
7	0.4201356489519669	0.31678914067161856
8	0.3062000787403324	0.26386269716106225
9	0.29373837795462304	0.28476454385277694
>10	6.774674849544765	20.996734025264473
>50	3.175044696188025	23.58137964076382
>100	1.3654383414500786	27.640376842231195
>500	0.07120930072751387	5.246283126392273
>1k	0.03115406906828732	7.4506674193704105
>5k	0.00267034877728177	1.706669584403064
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGAAGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	6826	0.6514570936386064	No Hit
TGAAGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	5916	0.5646088728341628	No Hit
TGAAGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	5058	0.4827234075042589	No Hit
TGAAGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	4802	0.4582913805526792	No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	4649	0.4436894269448991	No Hit
TGAAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	4169	0.3978793764106871	No Hit
TGAAGCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	4088	0.39014893038303883	No Hit
TGAAGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTA	3821	0.3646670897733834	No Hit
TGAAGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	3783	0.3610404607727583	No Hit
TGAAGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3571	0.34080768845348136	No Hit
TGAAGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	3187	0.30415964802611173	No Hit
TGAAGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	3092	0.29509307552454894	No Hit
TGAAGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	2771	0.2644576042297947	No Hit
TGAAGCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	2760	0.263407790571719	No Hit
TGAAGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	2452	0.2340130081455996	No Hit
TGAAGCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGC	2155	0.20566803937755596	No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	2116	0.2019459727716512	No Hit
TGAAGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	2110	0.2013733471399736	No Hit
TGAAGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	2092	0.19965547024494062	No Hit
TGAAGCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	2046	0.19526534040207863	No Hit
TGAAGCGGGAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTCGA	2039	0.19459727716512137	No Hit
TGAAGCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	1765	0.1684473733185087	No Hit
TGAAGCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	1671	0.15947623842222552	No Hit
TGAAGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1569	0.14974160268370545	No Hit
TGAAGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTT	1375	0.13122670725946145	No Hit
TGAAGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTC	1373	0.13103583204890223	No Hit
TGAAGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATC	1355	0.12931795515386926	No Hit
TGAAGCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAA	1323	0.12626395178492184	No Hit
TGAAGCGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1291	0.12320994841597435	No Hit
TGAAGCGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGT	1268	0.12101488349454337	No Hit
TGAAGCGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCT	1215	0.11595669041472412	No Hit
TGAAGCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGC	1184	0.11299812465105626	No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG	1169	0.11156656057186214	No Hit
TGAAGCGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAA	1150	0.10975324607154957	No Hit
TGAAGCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCT	1148	0.10956237086099035	No Hit
TGAAGCGGGGGCAGCGTGTCTCAGTCGGTTGTCGAGTCCCTTCTGTCCCA	1110	0.10593574186036524	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0015270016844737332	0.0	0.0	0.0	0.0
8	0.002099627316151383	0.0	0.0	0.0	0.0
9	0.0021950649214309914	0.0	0.0	0.0	0.0
10	0.008207634054046316	0.0	0.0	0.0	0.0
11	0.015174579239457723	0.0	0.0	0.0	0.0
12	0.022905025267106	0.0	0.0	0.0	0.0
13	0.030921784110593098	0.0	0.0	0.0	0.0
14	0.03827047971712294	0.0	0.0	0.0	0.0
15	0.04590548813949161	0.0	0.0	0.0	0.0
16	0.054399435009376745	0.0	0.0	0.0	0.0
17	0.061939005826465804	0.0	0.0	0.0	0.0
18	0.068715075801318	0.0	0.0	0.0	0.0
19	0.07434589451281488	0.0	0.0	0.0	0.0
20	0.07997671322431178	0.0	0.0	0.0	0.0
21	0.08427140546189416	0.0	0.0	0.0	0.0
22	0.09047484980506869	0.0	0.0	0.0	0.0
23	0.09725091977992088	0.0	0.0	0.0	0.0
24	0.10402698975477308	0.0	0.0	0.0	0.0
25	0.11099393494018447	0.0	0.0	0.0	0.0
26	0.11767456730975706	0.0	0.0	0.0	0.0
27	0.12359169883709278	0.0	0.0	0.0	0.0
28	0.13256283373337596	0.0	0.0	0.0	0.0
29	0.13895715328710972	0.0	0.0	0.0	0.0
30	0.14745110015699486	0.0	0.0	0.0	0.0
31	0.15508610857936353	0.0	0.0	0.0	0.0
32	0.1638663682650875	0.0	0.0	0.0	0.0
33	0.16921087416074557	0.0	0.0	0.0	0.0
34	0.17541431850392009	0.0	0.0	0.0	0.0
35	0.18743945676915075	0.0	0.0	0.0	0.0
36	0.19698321729711157	0.0	0.0	0.0	0.0
37	0.20433191290364142	0.0	0.0	0.0	0.0
38	0.21368479822104303	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	60	0.0	44.0	33
CTATGCG	20	7.8561634E-4	44.0	18
TCGTTTG	20	7.8561634E-4	44.0	19
CAAACTA	25	4.44257E-5	44.0	24
GGTACGA	20	7.8561634E-4	44.0	44
ACACGTT	25	4.44257E-5	44.0	17
CTCGTAA	35	1.4459147E-7	44.0	18
CAAAACG	20	7.8561634E-4	44.0	24
ATTACCA	30	2.5275021E-6	44.0	33
ACCGATA	25	4.44257E-5	44.0	27
TAAACGT	20	7.8561634E-4	44.0	36
TGTATCG	40	8.310963E-9	44.0	11
CACGATT	20	7.8561634E-4	44.0	29
GATCACG	20	7.8561634E-4	44.0	40
TAACGTG	20	7.8561634E-4	44.0	30
TATGCGT	20	7.8561634E-4	44.0	25
ATCACGT	20	7.8561634E-4	44.0	41
GTAATAC	30	2.5275021E-6	44.0	22
ATACTAC	25	4.44257E-5	44.0	42
TATTGCG	20	7.8561634E-4	44.0	18
>>END_MODULE