FastQCFastQC Report
Fri 17 Jun 2016
SRR1527524_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527524_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences819676
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA53270.6498909325147986No Hit
TGAAGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT44770.5461914219764883No Hit
TGAAGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC42780.5219135365681074No Hit
TGAAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA38450.46908778590565053No Hit
TGAAGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA36740.44822588437382577No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC33990.41467604272907826No Hit
TGAAGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG33650.41052806230754596No Hit
TGAAGCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG32120.39186215041065003No Hit
TGAAGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG30690.3744162327553814No Hit
TGAAGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG29700.3623382897632723No Hit
TGAAGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG28350.3458683675013054No Hit
TGAAGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC22770.27779268881850877No Hit
TGAAGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC22220.2710827204895593No Hit
TGAAGCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG21850.26656874179553874No Hit
TGAAGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG20130.24558484083955123No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT18020.21984296234121772No Hit
TGAAGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC17670.21557298249552265No Hit
TGAAGCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT17080.20837501646992226No Hit
TGAAGCGGGAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTCGAAG16500.20129904986848463No Hit
TGAAGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG15880.1937350855703961No Hit
TGAAGCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA15730.19190509420795532No Hit
TGAAGCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC14190.17311718288689676No Hit
TGAAGCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT12910.15750125659406888No Hit
TGAAGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG12370.1509132876892821No Hit
TGAAGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA11360.1385913458488476No Hit
TGAAGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT10710.13066138327827093No Hit
TGAAGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT10300.1256594068875995No Hit
TGAAGCGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC10260.12517140919094863No Hit
TGAAGCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC10210.12456141207013503No Hit
TGAAGCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA10150.12382941552515872No Hit
TGAAGCGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCG9610.11724144662037196No Hit
TGAAGCGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC9590.11699744777204653No Hit
TGAAGCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT9580.11687544834788381No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT9080.11077547713974789No Hit
TGAAGCGGGGGCAGCGTGTCTCAGTCGGTTGTCGAGTCCCTTCTGTCCCAGT8890.10845748808065625No Hit
TGAAGCGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGC8530.10406550881079842No Hit
TGAAGCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG8470.1033335122658221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGTA551.8189894E-1246.00000437
ACGTAAT551.8189894E-1246.00000439
CATATTA206.310389E-446.030
CGTATGG206.310389E-446.028
TACCATT206.310389E-446.045
CAAAACG206.310389E-446.024
CGAAAGT206.310389E-446.039
CGACCAA650.046.028
TGTATCG206.310389E-446.011
CACGATT206.310389E-446.029
CGTTCAT206.310389E-446.034
AAAACCG206.310389E-446.032
CGTTATC206.310389E-446.041
TAGCATA501.6370905E-1146.030
CTAACGG253.416292E-546.013
ATTTTCG301.8607443E-646.011
ATTCACC206.310389E-446.021
AATTGAC206.310389E-446.027
CGGATAG253.416292E-546.046
CGAATTG253.416292E-546.042
Produced by FastQC (version 0.11.5)