##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527524_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	819676
Sequences flagged as poor quality	0
Sequence length	52
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3007871402847	33.0	31.0	34.0	31.0	34.0
2	32.56964947125449	34.0	31.0	34.0	31.0	34.0
3	32.925117973443164	34.0	31.0	34.0	31.0	34.0
4	36.41299855064684	37.0	37.0	37.0	35.0	37.0
5	36.28836515891645	37.0	37.0	37.0	35.0	37.0
6	36.4208870334132	37.0	37.0	37.0	35.0	37.0
7	36.59275006221971	37.0	37.0	37.0	35.0	37.0
8	36.70807611788072	37.0	37.0	37.0	35.0	37.0
9	38.7513981134009	39.0	39.0	39.0	39.0	39.0
10	37.76707381941157	39.0	38.0	39.0	35.0	39.0
11	37.58831416315715	39.0	37.0	39.0	35.0	39.0
12	37.29719913722007	39.0	37.0	39.0	35.0	39.0
13	37.31331648114621	39.0	37.0	39.0	35.0	39.0
14	38.09192778610085	40.0	38.0	40.0	35.0	40.0
15	38.20146618907959	40.0	38.0	40.0	35.0	40.0
16	38.30494366066592	40.0	38.0	40.0	35.0	40.0
17	38.21247053713906	40.0	38.0	40.0	35.0	40.0
18	38.03989625168969	40.0	38.0	40.0	34.0	40.0
19	37.9461677540882	40.0	37.0	40.0	34.0	40.0
20	37.76763867674545	40.0	37.0	40.0	33.0	40.0
21	37.72280632835413	40.0	37.0	40.0	33.0	40.0
22	37.61983759436655	39.0	37.0	40.0	33.0	40.0
23	37.37337435767303	39.0	36.0	40.0	33.0	40.0
24	37.24158813970398	39.0	36.0	40.0	33.0	40.0
25	37.18568190358142	39.0	35.0	40.0	33.0	40.0
26	37.08189821344043	39.0	35.0	40.0	33.0	40.0
27	36.96318545376466	39.0	35.0	40.0	33.0	40.0
28	36.734587812745524	38.0	35.0	40.0	32.0	40.0
29	36.615654234112014	38.0	35.0	40.0	32.0	40.0
30	36.37620596430785	38.0	35.0	40.0	31.0	40.0
31	36.24690365461475	38.0	35.0	40.0	31.0	40.0
32	36.036731586626914	37.0	35.0	40.0	31.0	40.0
33	35.72108613647344	37.0	34.0	40.0	30.0	40.0
34	35.31886745494561	36.0	34.0	40.0	30.0	40.0
35	35.05353822729957	36.0	34.0	39.0	29.0	40.0
36	34.89043963712491	36.0	33.0	39.0	29.0	40.0
37	34.69586397552204	35.0	33.0	39.0	28.0	40.0
38	34.75720284600256	35.0	33.0	39.0	29.0	40.0
39	34.675410772061156	35.0	33.0	39.0	29.0	40.0
40	34.43237449919236	35.0	33.0	39.0	28.0	40.0
41	34.24800042943797	35.0	33.0	39.0	28.0	40.0
42	34.142097609299284	35.0	33.0	38.0	28.0	40.0
43	34.07364739238431	35.0	33.0	38.0	28.0	40.0
44	34.00180071150064	35.0	33.0	38.0	28.0	40.0
45	33.82509064557215	35.0	33.0	37.0	27.0	40.0
46	33.68922232687062	35.0	33.0	37.0	27.0	40.0
47	33.54162132354735	35.0	33.0	37.0	27.0	40.0
48	33.28609352963854	35.0	33.0	36.0	26.0	40.0
49	33.14668234765932	35.0	32.0	36.0	26.0	39.0
50	33.640554316583625	35.0	33.0	36.0	27.0	39.0
51	33.834291842142505	35.0	33.0	36.0	29.0	39.0
52	33.46591946086014	35.0	33.0	35.0	28.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	4.0
14	11.0
15	24.0
16	54.0
17	109.0
18	164.0
19	246.0
20	510.0
21	905.0
22	1280.0
23	1665.0
24	2379.0
25	3232.0
26	4321.0
27	5913.0
28	7780.0
29	10496.0
30	13768.0
31	19162.0
32	27873.0
33	43587.0
34	79541.0
35	112832.0
36	159727.0
37	153224.0
38	151452.0
39	19413.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.36340700471894	0.5590013615135736	0.03476983588637461	0.042821797881113996
8	99.72171931348484	0.18263313797158875	0.07234565852849174	0.023301890015079128
9	98.73340197834266	0.7657903854693806	0.25924877634577564	0.24155885984218153
10	63.44982651681884	27.894924335957132	3.020461743420571	5.634787403803454
11	36.70352675935369	25.757006426929664	18.404711129763466	19.134755683953173
12	29.739433629873268	16.50554609382244	26.397869402056422	27.35715087424787
13	25.139689340666312	16.54202392164709	30.16118563920378	28.15710109848282
14	25.710768645171996	17.508137361591654	29.26302587851785	27.518068114718496
15	25.947203529199342	17.10492926473387	28.01436177221244	28.93350543385435
16	30.83608645367194	17.62147482663882	23.45365729873755	28.08878142095169
17	31.898701438129212	18.54086248712906	22.363592443843665	27.19684363089806
18	30.227675325372466	18.561846388085048	22.640165138420546	28.57031314812194
19	31.157310937492376	17.650998687286197	23.048253212244838	28.143437162976593
20	29.730893670181878	19.963253773442187	22.68786691326817	27.61798564310776
21	33.22532317647461	18.123014459371753	23.281882109516445	25.3697802546372
22	32.057910686661565	18.075556683372454	22.593073360693737	27.273459269272248
23	30.60062756503789	20.29179822271239	22.07908978669621	27.02848442555351
24	29.5488705293311	19.713520952181103	21.999546162142114	28.73806235634568
25	29.364529399421237	20.074883246551074	22.800960379467007	27.759626974560682
26	29.273395829571687	20.766375982705362	21.44981675686491	28.510411430858046
27	30.326860857216754	18.570752346048927	21.307565428291177	29.79482136844314
28	31.349582029972815	19.49245799559826	21.06210258687579	28.09585738755313
29	31.249786501007716	19.84198634582445	19.908476031993132	28.99975112117471
30	30.687247156193422	19.448172204627195	21.46787267164099	28.396707967538394
31	32.827117056007495	19.46098214416428	20.05133735768767	27.66056344214055
32	33.76883061111952	18.28990967162635	21.23851375421508	26.702745963039053
33	33.6582991328281	18.971886452695944	21.39699100620245	25.972823408273516
34	32.22077991791878	21.347337240568223	22.621133228251164	23.810749613261827
35	30.81327256135351	23.397415564198536	23.004699417818745	22.784612456629205
36	32.85895890571396	21.82874696831431	20.580692859129705	24.73160126684202
37	31.145110995076102	22.653219076805957	19.88017216558738	26.32149776253056
38	34.06284922335166	21.038556698012385	20.127586997789372	24.771007080846577
39	31.832699749657184	20.194564681654704	20.916923272122155	27.05581229656596
40	31.426685666043657	21.151162166514574	20.643156564301016	26.778995603140753
41	29.024273005431418	20.260444370702572	21.808251065054975	28.907031558811042
42	27.924448196604512	18.506824647787663	24.581419975697717	28.98730717991011
43	28.914717522533294	17.220950717112615	26.2781879669528	27.5861437934013
44	28.696460552706192	18.009066997203774	24.314729234478012	28.979743215612025
45	28.947047369936413	18.536104509586714	24.99463202533684	27.522216095140028
46	29.30413968446069	20.015957524680484	23.235644327758774	27.444258463100052
47	29.25655990903723	20.21908656591141	24.14088005504614	26.38347347000522
48	29.216788096760183	20.426973584684678	23.71437006817328	26.641868250381854
49	29.378925331472434	20.54189704224596	23.144144759636735	26.93503286664487
50	27.373132798813188	21.042948677282244	23.546376861101216	28.03754166280335
51	27.911150259370775	22.5303656566741	22.400192271092482	27.158291812862643
52	28.93435942982349	22.237811037531902	21.981246248517706	26.8465832841269
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.5
6	3.0
7	10.0
8	17.0
9	9.5
10	2.0
11	21.5
12	41.0
13	108.5
14	248.5
15	321.0
16	297.0
17	273.0
18	453.0
19	633.0
20	649.5
21	666.0
22	615.0
23	564.0
24	643.0
25	722.0
26	976.5
27	1231.0
28	1446.0
29	1661.0
30	1823.0
31	1985.0
32	2880.0
33	3775.0
34	3866.5
35	3958.0
36	4973.5
37	5989.0
38	6303.5
39	8702.0
40	10786.0
41	12115.0
42	13444.0
43	14408.0
44	15372.0
45	16433.0
46	17494.0
47	21744.5
48	25995.0
49	26111.0
50	26227.0
51	29537.0
52	32847.0
53	39742.0
54	46637.0
55	49553.5
56	52470.0
57	60175.5
58	67881.0
59	74948.0
60	82015.0
61	81107.0
62	80199.0
63	81401.5
64	75799.5
65	68995.0
66	61141.5
67	53288.0
68	44277.0
69	35266.0
70	30127.5
71	24989.0
72	20676.0
73	16363.0
74	19251.5
75	22140.0
76	15885.5
77	9631.0
78	6526.0
79	3421.0
80	2595.5
81	1770.0
82	1265.0
83	760.0
84	537.5
85	315.0
86	210.5
87	106.0
88	63.5
89	12.5
90	4.0
91	2.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	819676.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	9.478745260322372
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.25394169508976	6.2800423582000695
2	10.016088551386833	1.8987990376685422
3	3.5883905013192616	1.020403183696973
4	1.6268743162365662	0.6168290885667019
5	0.912542634661175	0.43248795865683515
6	0.6628483171375249	0.3769782206627985
7	0.4878048780487805	0.3236644723036907
8	0.39127356972778166	0.2967025995637301
9	0.3526610463993822	0.30085057998526243
>10	10.77031984040157	28.468443628946073
>50	3.43908874444945	22.132403535055314
>100	1.3900508398223825	24.976820109409083
>500	0.06950254199111912	4.398811237610959
>1k	0.037325439217452856	7.82687305715917
>5k	0.0012870841109466503	0.6498909325147986
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGAAGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	5327	0.6498909325147986	No Hit
TGAAGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4477	0.5461914219764883	No Hit
TGAAGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4278	0.5219135365681074	No Hit
TGAAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	3845	0.46908778590565053	No Hit
TGAAGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	3674	0.44822588437382577	No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	3399	0.41467604272907826	No Hit
TGAAGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	3365	0.41052806230754596	No Hit
TGAAGCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	3212	0.39186215041065003	No Hit
TGAAGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	3069	0.3744162327553814	No Hit
TGAAGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	2970	0.3623382897632723	No Hit
TGAAGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	2835	0.3458683675013054	No Hit
TGAAGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	2277	0.27779268881850877	No Hit
TGAAGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	2222	0.2710827204895593	No Hit
TGAAGCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	2185	0.26656874179553874	No Hit
TGAAGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	2013	0.24558484083955123	No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1802	0.21984296234121772	No Hit
TGAAGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	1767	0.21557298249552265	No Hit
TGAAGCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT	1708	0.20837501646992226	No Hit
TGAAGCGGGAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTCGAAG	1650	0.20129904986848463	No Hit
TGAAGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1588	0.1937350855703961	No Hit
TGAAGCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	1573	0.19190509420795532	No Hit
TGAAGCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1419	0.17311718288689676	No Hit
TGAAGCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1291	0.15750125659406888	No Hit
TGAAGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1237	0.1509132876892821	No Hit
TGAAGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA	1136	0.1385913458488476	No Hit
TGAAGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	1071	0.13066138327827093	No Hit
TGAAGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	1030	0.1256594068875995	No Hit
TGAAGCGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC	1026	0.12517140919094863	No Hit
TGAAGCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	1021	0.12456141207013503	No Hit
TGAAGCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	1015	0.12382941552515872	No Hit
TGAAGCGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCG	961	0.11724144662037196	No Hit
TGAAGCGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC	959	0.11699744777204653	No Hit
TGAAGCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	958	0.11687544834788381	No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	908	0.11077547713974789	No Hit
TGAAGCGGGGGCAGCGTGTCTCAGTCGGTTGTCGAGTCCCTTCTGTCCCAGT	889	0.10845748808065625	No Hit
TGAAGCGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGC	853	0.10406550881079842	No Hit
TGAAGCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	847	0.1033335122658221	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0013419936657898975	0.0	0.0	0.0	0.0
8	0.002439988483254359	0.0	0.0	0.0	0.0
9	0.002927986179905231	0.0	0.0	0.0	0.0
10	0.009881953357180154	0.0	0.0	0.0	0.0
11	0.017323918231105948	0.0	0.0	0.0	0.0
12	0.02610787677082164	0.0	0.0	0.0	0.0
13	0.0359898301280018	0.0	0.0	0.0	0.0
14	0.043065796729439436	0.0	0.0	0.0	0.0
15	0.0536797466315959	0.0	0.0	0.0	0.0
16	0.06429369653375236	0.0	0.0	0.0	0.0
17	0.07112566428686456	0.0	0.0	0.0	0.0
18	0.07929962570576667	0.0	0.0	0.0	0.0
19	0.08783958539715693	0.0	0.0	0.0	0.0
20	0.09430555487778097	0.0	0.0	0.0	0.0
21	0.09967352954094057	0.0	0.0	0.0	0.0
22	0.10443150708328656	0.0	0.0	0.0	0.0
23	0.1087014869289817	0.0	0.0	0.0	0.0
24	0.11406946159214129	0.0	0.0	0.0	0.0
25	0.12065743049692805	0.0	0.0	0.0	0.0
26	0.12883139191583015	0.0	0.0	0.0	0.0
27	0.13578535909310507	0.0	0.0	0.0	0.0
28	0.14456931763282077	0.0	0.0	0.0	0.0
29	0.1523772807792347	0.0	0.0	0.0	0.0
30	0.16006324450148596	0.0	0.0	0.0	0.0
31	0.16762720879957446	0.0	0.0	0.0	0.0
32	0.17763116158091735	0.0	0.0	0.0	0.0
33	0.185561124151494	0.0	0.0	0.0	0.0
34	0.19361308614623338	0.0	0.0	0.0	0.0
35	0.20605702741083062	0.0	0.0	0.0	0.0
36	0.21569498191968534	0.0	0.0	0.0	0.0
37	0.22374694391442473	0.0	0.0	0.0	0.0
38	0.23399489554409303	0.0	0.0	0.0	0.0
39	0.24351085062878502	0.0	0.0	0.0	0.0
40	0.2515628126235244	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACGTA	55	1.8189894E-12	46.000004	37
ACGTAAT	55	1.8189894E-12	46.000004	39
CATATTA	20	6.310389E-4	46.0	30
CGTATGG	20	6.310389E-4	46.0	28
TACCATT	20	6.310389E-4	46.0	45
CAAAACG	20	6.310389E-4	46.0	24
CGAAAGT	20	6.310389E-4	46.0	39
CGACCAA	65	0.0	46.0	28
TGTATCG	20	6.310389E-4	46.0	11
CACGATT	20	6.310389E-4	46.0	29
CGTTCAT	20	6.310389E-4	46.0	34
AAAACCG	20	6.310389E-4	46.0	32
CGTTATC	20	6.310389E-4	46.0	41
TAGCATA	50	1.6370905E-11	46.0	30
CTAACGG	25	3.416292E-5	46.0	13
ATTTTCG	30	1.8607443E-6	46.0	11
ATTCACC	20	6.310389E-4	46.0	21
AATTGAC	20	6.310389E-4	46.0	27
CGGATAG	25	3.416292E-5	46.0	46
CGAATTG	25	3.416292E-5	46.0	42
>>END_MODULE