FastQCFastQC Report
Fri 17 Jun 2016
SRR1527523_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527523_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1125816
Sequences flagged as poor quality0
Sequence length52
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAAGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA74690.6634299032879263No Hit
TGAAGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT61980.5505340126628152No Hit
TGAAGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC57660.512161845274894No Hit
TGAAGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA52800.4689931569634825No Hit
TGAAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA49010.4353286860375052No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC47710.42378150603651044No Hit
TGAAGCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG45070.4003318481883363No Hit
TGAAGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG43450.3859422854178658No Hit
TGAAGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG42560.37803690834026166No Hit
TGAAGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG42360.37626041910933933No Hit
TGAAGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG35620.3163927320272584No Hit
TGAAGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC33760.29987138217968123No Hit
TGAAGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC32280.28672536187085634No Hit
TGAAGCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG29870.26531866663824283No Hit
TGAAGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG28180.25030733263694954No Hit
TGAAGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC26520.2355624720202946No Hit
TGAAGCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT23100.2051845061715236No Hit
TGAAGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG23080.20500685724843137No Hit
TGAAGCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA22040.19576911324763552No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT21140.18777491170848523No Hit
TGAAGCGGGAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTCGAAG20500.18209014616953392No Hit
TGAAGCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC18580.16503584955268003No Hit
TGAAGCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT17940.1593510840137287No Hit
TGAAGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG16370.14540564355098878No Hit
TGAAGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA15470.13741144201183852No Hit
TGAAGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT14940.13270374554989448No Hit
TGAAGCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA14720.13074960739587996No Hit
TGAAGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT14570.12941724047268827No Hit
TGAAGCGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC13850.12302187924136804No Hit
TGAAGCGGGGGCAGCGTGTCTCAGTCGGTTGTCGAGTCCCTTCTGTCCCAGT13300.11813653385633176No Hit
TGAAGCGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCG13300.11813653385633176No Hit
TGAAGCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT12950.11502767770221776No Hit
TGAAGCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC12910.11467237985603332No Hit
TGAAGCGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC12830.1139617841636644No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT12810.11378413524057217No Hit
TGAAGCGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGC12450.11058645462491207No Hit
TGAAGCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG11990.10650052939379083No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGCG206.3113973E-446.00000413
TAGACGT206.3113973E-446.00000426
TTTAACG206.3113973E-446.00000442
TAAACGT405.6115823E-946.00000436
GCCGATA206.3113973E-446.00000430
TAATTCG206.3113973E-446.00000416
CTCATAA405.6115823E-946.00000445
CGAATAG206.3113973E-446.00000440
TATACAA206.3113973E-446.00000422
CGATCTA206.3113973E-446.00000442
TATAACG351.01954356E-746.00000422
AATAACC206.3113973E-446.00000441
ACGAAAT206.3113973E-446.00000441
TCAAACG206.3113973E-446.00000432
AACGTAA750.046.038
GATACTA253.4171113E-546.09
CTATATG253.4171113E-546.036
CACGATT253.4171113E-546.029
GATCACG301.8613682E-646.040
TATGCGT253.4171113E-546.025
Produced by FastQC (version 0.11.5)