##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527523_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1125816
Sequences flagged as poor quality	0
Sequence length	52
%GC	59
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.499333816538403	31.0	31.0	34.0	30.0	34.0
2	31.696930048960045	31.0	31.0	34.0	30.0	34.0
3	32.218115571283406	34.0	31.0	34.0	30.0	34.0
4	35.8536688055597	37.0	35.0	37.0	35.0	37.0
5	35.79561491398239	37.0	35.0	37.0	35.0	37.0
6	35.997784717929036	37.0	35.0	37.0	35.0	37.0
7	36.38455928855159	37.0	35.0	37.0	35.0	37.0
8	36.547954550299515	37.0	37.0	37.0	35.0	37.0
9	38.63756954955339	39.0	39.0	39.0	38.0	39.0
10	37.22313326511615	39.0	37.0	39.0	34.0	39.0
11	36.85104670745486	39.0	37.0	39.0	32.0	39.0
12	36.551778443369074	39.0	35.0	39.0	32.0	39.0
13	36.522553419031176	39.0	35.0	39.0	32.0	39.0
14	37.313906535348586	39.0	37.0	40.0	33.0	40.0
15	37.44674085285695	39.0	37.0	40.0	33.0	40.0
16	37.52264579647118	40.0	37.0	40.0	33.0	40.0
17	37.467343686712574	39.0	37.0	40.0	33.0	40.0
18	37.3194971469583	39.0	36.0	40.0	33.0	40.0
19	37.1722013188656	39.0	36.0	40.0	32.0	40.0
20	36.93810178572698	39.0	35.0	40.0	31.0	40.0
21	36.97598719506562	39.0	35.0	40.0	32.0	40.0
22	36.87765673964484	39.0	35.0	40.0	31.0	40.0
23	36.73054566643217	38.0	35.0	40.0	31.0	40.0
24	36.63377585680075	38.0	35.0	40.0	31.0	40.0
25	36.54588049912241	38.0	35.0	40.0	31.0	40.0
26	36.3818652426329	38.0	35.0	40.0	31.0	40.0
27	36.216319540670945	38.0	35.0	40.0	31.0	40.0
28	35.944030818535175	38.0	34.0	40.0	30.0	40.0
29	35.85438739545361	38.0	34.0	40.0	30.0	40.0
30	35.678201411243045	37.0	34.0	40.0	30.0	40.0
31	35.52477136583598	37.0	34.0	40.0	30.0	40.0
32	35.32285115862628	37.0	34.0	40.0	29.0	40.0
33	35.08610376828896	36.0	33.0	39.0	29.0	40.0
34	34.78908720430337	36.0	33.0	39.0	28.0	40.0
35	34.563386024003925	35.0	33.0	39.0	28.0	40.0
36	34.41444339039417	35.0	33.0	39.0	28.0	40.0
37	34.28846099184947	35.0	33.0	39.0	27.0	40.0
38	34.0801543058546	35.0	33.0	38.0	27.0	40.0
39	33.89318059078926	35.0	33.0	38.0	26.0	40.0
40	34.168964555486866	35.0	33.0	38.0	27.0	40.0
41	34.247695893467494	35.0	33.0	38.0	28.0	40.0
42	34.34473572946201	35.0	33.0	38.0	29.0	40.0
43	34.28407839291678	35.0	33.0	38.0	29.0	40.0
44	34.227425263098056	35.0	33.0	37.0	29.0	40.0
45	34.12949096477577	35.0	33.0	37.0	29.0	40.0
46	33.87357703212603	35.0	33.0	37.0	28.0	40.0
47	33.81846855969359	35.0	33.0	37.0	28.0	40.0
48	33.68688666709303	35.0	33.0	36.0	28.0	40.0
49	33.56627903671648	35.0	33.0	36.0	27.0	39.0
50	33.41483332978036	35.0	33.0	36.0	27.0	39.0
51	33.283588970133664	35.0	33.0	35.0	27.0	39.0
52	32.79126695658971	35.0	32.0	35.0	25.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	10.0
15	24.0
16	64.0
17	120.0
18	272.0
19	504.0
20	942.0
21	1495.0
22	2440.0
23	3350.0
24	4594.0
25	6412.0
26	8887.0
27	11929.0
28	15669.0
29	20184.0
30	26219.0
31	35263.0
32	48852.0
33	71813.0
34	117047.0
35	157974.0
36	213949.0
37	194042.0
38	168247.0
39	15512.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	100.0	0.0
2	100.0	0.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	99.3851570771778	0.5428062845083033	0.026292040617649774	0.045744597696248765
8	99.73734606720814	0.17605008278439815	0.06439773462093273	0.022206115386528526
9	98.725813099121	0.768331592373887	0.2507514549446801	0.2551038535604397
10	63.61208225855734	27.71065609300277	2.94435325133059	5.732908397109297
11	36.371751689441254	25.845253576072825	18.527805609442396	19.255189125043522
12	29.457211480384004	16.586191704505886	26.376246207195493	27.580350607914617
13	24.972197943536067	16.629893339586577	30.188503272293165	28.209405444584196
14	25.535966800969252	17.477012229351864	29.497804259310577	27.489216710368304
15	25.981243826699924	17.06548849900872	28.00519800748968	28.94806966680168
16	30.620101330945733	17.728385455527366	23.46138267709821	28.190130536428686
17	31.617244736262407	18.60010872114093	22.431374221009474	27.35127232158719
18	29.96981744796663	18.530825641134964	22.688698686108566	28.810658224789844
19	30.92672337220292	17.75370042706801	23.093116459528023	28.226459741201047
20	29.562201993931513	19.98310558741393	22.616484398871574	27.838208019782982
21	33.13072473654665	18.09913875802085	23.334541346010358	25.435595159422142
22	32.05275107122301	18.04699879909328	22.65663305549042	27.243617074193295
23	30.43703411569919	20.457872334377907	21.920900040503955	27.184193509418947
24	29.381799512531355	19.929455612640076	21.94230673573657	28.746438139092
25	29.329748378065336	20.146364947735687	22.714724253341576	27.809162420857405
26	29.119856175431863	20.898885785954366	21.49827325246754	28.482984786146226
27	30.260539910607058	18.74302727976863	21.14111009259062	29.85532271703369
28	31.33949064500771	19.610842269074162	21.02110824504182	28.028558840876304
29	31.268964022540096	19.967650131104904	19.75935676877927	29.004029077575733
30	30.636445031870217	19.560034677069787	21.411402929075443	28.392117361984553
31	32.74993426989845	19.662982228001734	19.829883391246884	27.757200110852924
32	33.5865718732013	18.417929750509852	21.21492322013544	26.780575156153404
33	33.481670184115345	19.019004881792405	21.491167295543853	26.008157638548397
34	31.817899194895084	21.571642257704635	22.72120843903444	23.889250108365843
35	30.601092896174865	23.537682889566327	23.101377134451813	22.759847079807003
36	32.78004576236258	21.980145956355212	20.52280301576812	24.717005265514082
37	31.011461908517912	22.84627328089137	19.765485656625952	26.37677915396477
38	33.77923213029483	21.214745571212347	20.076193623114257	24.92982867537857
39	31.78858712258486	20.182338854661865	20.70400491732219	27.325069105431083
40	31.1487845260682	21.30703418675876	20.621398168084305	26.922783119088734
41	29.072157439581602	20.420121938220813	21.66366439986641	28.844056222331183
42	27.61454802560987	18.648962174991297	24.478511586262762	29.25797821313607
43	28.74208574047624	17.324234155492547	26.167242249177487	27.766437854853727
44	28.436174294911424	18.10731060848309	24.23655375301115	29.219961343594335
45	28.87985248033426	18.432941084511146	25.093620982469606	27.593585452684987
46	29.254602883597318	20.254020195129577	23.21231888692291	27.279058034350196
47	29.067805040965844	20.283421091901342	24.11539718746225	26.533376679670567
48	29.074111577735618	20.571656469618482	23.656974141422754	26.697257811223146
49	29.282049642215068	20.60167913762107	23.149786465994442	26.966484754169418
50	27.018447064173895	21.11659454120389	23.57934156203145	28.285616832590758
51	27.863434166862078	22.557682605328047	22.355251657464454	27.223631570345418
52	28.760383579554738	22.400729781776064	21.736322809411128	27.10256382925807
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	2.0
7	10.0
8	18.0
9	13.0
10	8.0
11	33.5
12	59.0
13	156.5
14	330.5
15	407.0
16	402.5
17	398.0
18	559.0
19	720.0
20	756.5
21	793.0
22	825.0
23	857.0
24	990.0
25	1123.0
26	1419.0
27	1715.0
28	2031.5
29	2348.0
30	2587.5
31	2827.0
32	4030.0
33	5233.0
34	5407.0
35	5581.0
36	7073.5
37	8566.0
38	8808.5
39	12175.5
40	15300.0
41	17031.5
42	18763.0
43	20146.0
44	21529.0
45	22719.5
46	23910.0
47	29894.5
48	35879.0
49	36022.5
50	36166.0
51	40780.5
52	45395.0
53	54396.0
54	63397.0
55	67910.5
56	72424.0
57	82374.0
58	92324.0
59	102242.5
60	112161.0
61	111233.0
62	110305.0
63	111937.0
64	104127.5
65	94686.0
66	84201.0
67	73716.0
68	60927.0
69	48138.0
70	41220.0
71	34302.0
72	28388.0
73	22474.0
74	26510.5
75	30547.0
76	21779.0
77	13011.0
78	8652.0
79	4293.0
80	3275.5
81	2258.0
82	1584.0
83	910.0
84	574.0
85	238.0
86	187.0
87	136.0
88	76.0
89	12.0
90	8.0
91	4.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1125816.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	8.88448218572683
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.1089999999755	6.05113197187451
2	10.413999999996253	1.8504599496425185
3	3.9959999999985625	1.0650717244245493
4	1.8970000000352396	0.6741545082654753
5	1.0259999999996714	0.4557739361276404
6	0.5989999999997846	0.3193082897549075
7	0.4349999999998435	0.2705324825552846
8	0.3259999999998827	0.23170729540367235
9	0.26199999999990575	0.20949608993936328
>10	6.930999999997507	17.84776972843464
>50	4.024999999998552	24.979965492667997
>100	1.8339999999993404	29.964515378146384
>500	0.10199999999996331	6.166186016179631
>1k	0.03999999999998561	7.718305054025549
>5k	0.003999999999998561	2.1956220825578816
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TGAAGCGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	7469	0.6634299032879263	No Hit
TGAAGCGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	6198	0.5505340126628152	No Hit
TGAAGCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	5766	0.512161845274894	No Hit
TGAAGCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	5280	0.4689931569634825	No Hit
TGAAGCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	4901	0.4353286860375052	No Hit
TGAAGCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4771	0.42378150603651044	No Hit
TGAAGCGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	4507	0.4003318481883363	No Hit
TGAAGCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4345	0.3859422854178658	No Hit
TGAAGCGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	4256	0.37803690834026166	No Hit
TGAAGCGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	4236	0.37626041910933933	No Hit
TGAAGCGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	3562	0.3163927320272584	No Hit
TGAAGCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	3376	0.29987138217968123	No Hit
TGAAGCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	3228	0.28672536187085634	No Hit
TGAAGCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAG	2987	0.26531866663824283	No Hit
TGAAGCGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	2818	0.25030733263694954	No Hit
TGAAGCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2652	0.2355624720202946	No Hit
TGAAGCGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCGT	2310	0.2051845061715236	No Hit
TGAAGCGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	2308	0.20500685724843137	No Hit
TGAAGCGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	2204	0.19576911324763552	No Hit
TGAAGCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	2114	0.18777491170848523	No Hit
TGAAGCGGGAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTCGAAG	2050	0.18209014616953392	No Hit
TGAAGCGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1858	0.16503584955268003	No Hit
TGAAGCGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1794	0.1593510840137287	No Hit
TGAAGCGGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCG	1637	0.14540564355098878	No Hit
TGAAGCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA	1547	0.13741144201183852	No Hit
TGAAGCGGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGT	1494	0.13270374554989448	No Hit
TGAAGCGGGGCTCTTTTTCGTGACGCCTCCCAGGCGCTCGGCTGTGTCAAGA	1472	0.13074960739587996	No Hit
TGAAGCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	1457	0.12941724047268827	No Hit
TGAAGCGGGGGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTACTC	1385	0.12302187924136804	No Hit
TGAAGCGGGGGCAGCGTGTCTCAGTCGGTTGTCGAGTCCCTTCTGTCCCAGT	1330	0.11813653385633176	No Hit
TGAAGCGGGGAGTCCGGAGTAGGCGAGCGAGCGAGGCAGAGGTTTTTGGTCG	1330	0.11813653385633176	No Hit
TGAAGCGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	1295	0.11502767770221776	No Hit
TGAAGCGGGGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGC	1291	0.11467237985603332	No Hit
TGAAGCGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTGC	1283	0.1139617841636644	No Hit
TGAAGCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGAT	1281	0.11378413524057217	No Hit
TGAAGCGGGGACAGGAGCCACTGCCAGGATCAAGAGTCACCGCTTCGCAAGC	1245	0.11058645462491207	No Hit
TGAAGCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTG	1199	0.10650052939379083	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	8.882446154611411E-4	0.0	0.0	0.0	0.0
8	0.001598840307830054	0.0	0.0	0.0	0.0
9	0.001687664769376168	0.0	0.0	0.0	0.0
10	0.007550079231419699	0.0	0.0	0.0	0.0
11	0.013590142616555459	0.0	0.0	0.0	0.0
12	0.02007432830942179	0.0	0.0	0.0	0.0
13	0.027357934156203145	0.0	0.0	0.0	0.0
14	0.03366447092597725	0.0	0.0	0.0	0.0
15	0.04077042784966638	0.0	0.0	0.0	0.0
16	0.04796520923490162	0.0	0.0	0.0	0.0
17	0.05524881508168297	0.0	0.0	0.0	0.0
18	0.061111229543726504	0.0	0.0	0.0	0.0
19	0.06812836200586952	0.0	0.0	0.0	0.0
20	0.07336900523709025	0.0	0.0	0.0	0.0
21	0.07798787723748819	0.0	0.0	0.0	0.0
22	0.08296204708407058	0.0	0.0	0.0	0.0
23	0.08846916369992965	0.0	0.0	0.0	0.0
24	0.09459805154661152	0.0	0.0	0.0	0.0
25	0.1001051681624706	0.0	0.0	0.0	0.0
26	0.1057899337014219	0.0	0.0	0.0	0.0
27	0.11031998124027372	0.0	0.0	0.0	0.0
28	0.11840300724097011	0.0	0.0	0.0	0.0
29	0.12293305477982193	0.0	0.0	0.0	0.0
30	0.13083843185742608	0.0	0.0	0.0	0.0
31	0.13812203770420745	0.0	0.0	0.0	0.0
32	0.14620506370490383	0.0	0.0	0.0	0.0
33	0.15224512709003957	0.0	0.0	0.0	0.0
34	0.1606834509369204	0.0	0.0	0.0	0.0
35	0.17152003524554635	0.0	0.0	0.0	0.0
36	0.18235661955417226	0.0	0.0	0.0	0.0
37	0.19097259232414532	0.0	0.0	0.0	0.0
38	0.19745677801701167	0.0	0.0	0.0	0.0
39	0.2083821867871837	0.0	0.0	0.0	0.0
40	0.2160210904801495	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGGCG	20	6.3113973E-4	46.000004	13
TAGACGT	20	6.3113973E-4	46.000004	26
TTTAACG	20	6.3113973E-4	46.000004	42
TAAACGT	40	5.6115823E-9	46.000004	36
GCCGATA	20	6.3113973E-4	46.000004	30
TAATTCG	20	6.3113973E-4	46.000004	16
CTCATAA	40	5.6115823E-9	46.000004	45
CGAATAG	20	6.3113973E-4	46.000004	40
TATACAA	20	6.3113973E-4	46.000004	22
CGATCTA	20	6.3113973E-4	46.000004	42
TATAACG	35	1.01954356E-7	46.000004	22
AATAACC	20	6.3113973E-4	46.000004	41
ACGAAAT	20	6.3113973E-4	46.000004	41
TCAAACG	20	6.3113973E-4	46.000004	32
AACGTAA	75	0.0	46.0	38
GATACTA	25	3.4171113E-5	46.0	9
CTATATG	25	3.4171113E-5	46.0	36
CACGATT	25	3.4171113E-5	46.0	29
GATCACG	30	1.8613682E-6	46.0	40
TATGCGT	25	3.4171113E-5	46.0	25
>>END_MODULE