FastQCFastQC Report
Fri 17 Jun 2016
SRR1527522_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527522_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1587064
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC132980.8378994167847043No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG106970.6740118860991113No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC104070.6557391510361271No Hit
AAAGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT67540.42556569867377747No Hit
AAAGTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC51270.3230493540273108No Hit
AAAGTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC46560.2933719119077744No Hit
AAAGTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT46270.29154463840147593No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT45700.28795310082013076No Hit
AAAGTTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG44460.2801399313449237No Hit
AAAGTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT39330.2478160931128171No Hit
AAAGTTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA37570.23672643321252326No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC26790.16880226632322326No Hit
AAAGTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC26060.16420257784185138No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG24650.15531824803536592No Hit
AAAGTTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG23690.14926934263520564No Hit
AAAGTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA20220.12740506999087622No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAGTTGGGA19670.12393955127203439No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAA19630.12368751354702773No Hit
AAAGTTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT18910.1191508344969075No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTG17730.11171572160921046No Hit
AAAGTTGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT17310.10906932549664035No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGC17000.10711603312783859No Hit
AAAGTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC16980.10699001426533523No Hit
AAAGTTGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTC16220.10220129749020834No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAACG207.8573875E-444.00000427
CGACAAT207.8573875E-444.00000430
ACCGTAC207.8573875E-444.00000444
CGAACAA351.4465513E-744.018
AATTTCG750.044.042
TCCGATT254.4436092E-544.019
ACCGATT351.4465513E-744.035
CGCGATT254.4436092E-544.015
TTATCGT302.5283298E-644.039
AATTGCG700.044.043
CTACGCG254.4436092E-544.040
ATTATCG302.5283298E-644.038
CCAACGG254.4436092E-544.032
GGCGATA254.4436092E-544.020
TATACGG254.4436092E-544.034
TATCGTT351.4465513E-744.040
CGTCAAA254.4436092E-544.034
TCGGTTA502.7284841E-1144.019
TACGTCG502.7284841E-1144.044
GTACGGT302.5283298E-644.012
Produced by FastQC (version 0.11.5)