##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527522_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1587064
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.136682578648372	31.0	30.0	33.0	26.0	33.0
2	30.871136261675648	31.0	31.0	33.0	28.0	34.0
3	31.928947414849056	31.0	31.0	33.0	30.0	34.0
4	35.942311715217535	37.0	35.0	37.0	35.0	37.0
5	35.212844598579515	35.0	35.0	37.0	33.0	37.0
6	35.608577221838566	35.0	35.0	37.0	35.0	37.0
7	36.34816743370148	37.0	36.0	37.0	35.0	37.0
8	36.648828906710754	37.0	37.0	37.0	35.0	37.0
9	38.598579515381864	39.0	39.0	39.0	38.0	39.0
10	37.23368307768307	39.0	37.0	39.0	34.0	39.0
11	37.2756687821033	39.0	37.0	39.0	34.0	39.0
12	37.04570830161859	39.0	37.0	39.0	34.0	39.0
13	37.00736769279626	39.0	37.0	39.0	34.0	39.0
14	37.688267769919804	40.0	37.0	40.0	33.0	40.0
15	37.7515809066301	40.0	37.0	40.0	33.0	40.0
16	37.86918674987272	40.0	37.0	40.0	34.0	40.0
17	37.82186477671978	40.0	37.0	40.0	34.0	40.0
18	37.678086706018156	39.0	37.0	40.0	34.0	40.0
19	37.58187256468548	39.0	36.0	40.0	34.0	40.0
20	37.41495112988512	39.0	36.0	40.0	33.0	40.0
21	37.33146615385391	39.0	36.0	40.0	33.0	40.0
22	37.169656044116685	38.0	36.0	40.0	33.0	40.0
23	36.97989495067622	38.0	35.0	40.0	33.0	40.0
24	36.74271863012456	38.0	35.0	40.0	32.0	40.0
25	36.50333067853596	38.0	35.0	40.0	32.0	40.0
26	35.82998480212518	38.0	34.0	39.0	31.0	40.0
27	35.762329685507325	37.0	34.0	39.0	31.0	40.0
28	35.60822373892924	37.0	34.0	39.0	31.0	40.0
29	35.27138666115544	36.0	34.0	38.0	30.0	40.0
30	35.011991324861505	36.0	34.0	38.0	30.0	40.0
31	34.82532210421256	36.0	33.0	38.0	30.0	39.0
32	34.727082839759454	36.0	33.0	38.0	30.0	39.0
33	34.182813673550655	35.0	33.0	38.0	28.0	39.0
34	33.69312390678637	35.0	32.0	38.0	27.0	39.0
35	33.49936423483867	35.0	32.0	38.0	26.0	39.0
36	33.18806739992842	35.0	31.0	38.0	26.0	39.0
37	32.89690963943483	34.0	31.0	37.0	25.0	38.0
38	32.39067800668404	34.0	31.0	37.0	24.0	38.0
39	31.611566389257145	34.0	30.0	36.0	23.0	38.0
40	31.374866420005745	33.0	29.0	36.0	22.0	38.0
41	30.979786574454465	33.0	29.0	36.0	21.0	38.0
42	30.583926672144287	33.0	29.0	35.0	21.0	38.0
43	30.10612048411406	32.0	27.0	35.0	20.0	38.0
44	29.60712674473115	31.0	27.0	35.0	19.0	37.0
45	29.040257355721003	31.0	26.0	34.0	18.0	37.0
46	28.94564302384781	31.0	26.0	34.0	18.0	37.0
47	28.45496967986168	31.0	25.0	34.0	18.0	36.0
48	27.96898990840949	30.0	24.0	33.0	17.0	36.0
49	27.457696097951942	29.0	24.0	33.0	15.0	35.0
50	30.87140468185278	33.0	29.0	36.0	22.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	5.0
13	6.0
14	17.0
15	44.0
16	88.0
17	238.0
18	473.0
19	893.0
20	1470.0
21	2253.0
22	3477.0
23	5117.0
24	7480.0
25	10693.0
26	15315.0
27	21230.0
28	30311.0
29	40555.0
30	53174.0
31	66931.0
32	101530.0
33	157373.0
34	214458.0
35	387050.0
36	400132.0
37	65496.0
38	1251.0
39	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.57714370687005	0.24151514998765017	0.15815367244169107	0.023187470700614467
8	99.34129940569505	0.5087381479259815	0.08575583593352254	0.06420661044545148
9	96.85047357888529	1.5885937807170978	0.6111914831411965	0.9497411572564181
10	52.53108885337957	34.30649299587162	4.401145763497881	8.761272387250923
11	34.239072904432334	25.08764611887107	22.99321262406557	17.68006835263102
12	33.855660515265924	18.391948906912386	24.93774668192335	22.81464389589834
13	26.062906095784417	18.44443576314503	28.735703160049	26.756954981021558
14	26.126104555329842	20.17171330204705	29.327361719502175	24.374820423120934
15	26.694071568632395	19.883571172933166	27.957410665228373	25.464946593206072
16	30.70512594325119	22.868138903031003	25.323301391752317	21.10343376196549
17	32.75620894935554	21.879520926692308	22.70078585362657	22.663484270325583
18	30.27281823543348	22.35127253847356	23.92751647066533	23.448392755427633
19	28.685106586753907	23.654874661639354	24.5133151530121	23.146703598594637
20	27.2791771472354	26.313431594441056	22.978972492602693	23.428418765720853
21	28.0526809252809	25.145740814485112	26.61354551549276	20.188032744741232
22	27.067024392211025	24.828236290407947	25.225447745018474	22.87929157236255
23	29.82047352847774	24.646454081246883	25.012664895681585	20.52040749459379
24	30.51760987584622	24.84795824238972	22.822835121961056	21.811596759803006
25	29.332276455139805	22.025387760039923	23.377192098113245	25.265143686707027
26	26.64177374069351	22.45549013776382	24.77883689630664	26.123899225236038
27	25.863985321322897	22.271628617371448	24.489560597430224	27.374825463875435
28	29.17847043345448	24.773355075787745	21.582179420615677	24.4659950701421
29	30.077552007984554	25.020415055725543	21.77769768578961	23.124335250500295
30	30.423977861006236	23.614737653932043	22.941985956457962	23.019298528603763
31	31.411965743032415	23.461624735990483	22.889184053069066	22.237225467908036
32	32.85261337917059	22.247117948614548	23.17549890867665	21.724769763538205
33	32.84511525685165	22.631538488680984	23.412288351320427	21.111057903146943
34	28.88339726690291	25.030811611882065	26.034551851721165	20.05123926949386
35	27.643938744751313	28.7414370182929	24.533037104993877	19.08158713196191
36	28.453609936335273	28.049656472580814	22.57394786851696	20.922785722566953
37	27.718919967940803	30.366639278567213	21.700700160800068	20.213740592691913
38	29.86949486599154	27.86144730143208	21.41967809741762	20.849379735158756
39	28.876340210602724	25.930208233568404	20.884791035522195	24.30866052030668
40	28.171075646602784	26.084014255253727	22.022111269614836	23.722798828528653
41	24.976434472711876	26.842143732073815	21.574618288865477	26.606803506348832
42	23.963494855910035	25.705012526274935	23.50264387573532	26.828848742079714
43	24.5735521692887	24.831827827989294	23.746175327523023	26.848444675198984
44	26.271215275502435	23.439571435052397	22.913316665238455	27.375896624206714
45	26.015144946895653	25.11593735350307	23.081614856111663	25.787302843489613
46	26.047720822852767	26.015964069501923	22.752327568390438	25.183987539254876
47	25.917165281299305	25.68686581007445	23.757391006285822	24.638577902340423
48	25.49808955404445	26.060007661946837	23.698666216359264	24.743236567649447
49	27.37583361477546	24.44261857114773	23.390676116401103	24.79087169767571
50	25.607095870109838	24.717087653680004	23.395716870901236	26.28009960530892
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	33.0
2	66.0
3	51.5
4	37.0
5	50.0
6	63.0
7	939.5
8	1816.0
9	2194.0
10	2572.0
11	3251.0
12	3930.0
13	3583.5
14	3237.0
15	3580.0
16	3923.0
17	4693.5
18	5464.0
19	6355.0
20	7246.0
21	8991.5
22	10737.0
23	13040.0
24	15343.0
25	17285.5
26	19228.0
27	20369.0
28	21510.0
29	24632.0
30	27754.0
31	28437.5
32	29121.0
33	31169.5
34	33218.0
35	36492.5
36	39767.0
37	42759.0
38	45751.0
39	45862.0
40	45973.0
41	52300.5
42	58628.0
43	59857.5
44	61087.0
45	64687.5
46	68288.0
47	76702.5
48	85117.0
49	90640.5
50	96164.0
51	100191.5
52	104219.0
53	113030.0
54	121841.0
55	134450.5
56	147060.0
57	140256.5
58	133453.0
59	130004.0
60	126555.0
61	110195.5
62	93836.0
63	72112.5
64	50389.0
65	41669.0
66	32949.0
67	32504.0
68	32059.0
69	26678.5
70	21298.0
71	22506.5
72	23715.0
73	16504.0
74	9293.0
75	5872.0
76	2451.0
77	1881.5
78	1312.0
79	822.5
80	333.0
81	243.5
82	154.0
83	114.0
84	74.0
85	52.0
86	30.0
87	15.5
88	1.0
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1587064.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.336584256205983
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.52340155461765	9.938876238922303
2	8.950489512373663	2.387379150321187
3	3.2577632420995455	1.3034230189509406
4	1.5008061015273417	0.8006250810098968
5	0.8699642969514232	0.5801176073091827
6	0.6312687087840114	0.5051380993802596
7	0.47638172752283264	0.44473135330576524
8	0.3948301342298131	0.42125482816360166
9	0.3345902183017383	0.40160615739151373
>10	5.770497794462779	20.44369827516403
>50	2.0732006874346403	19.095286164388362
>100	1.1299254034331498	29.778414286170623
>500	0.06158021843805506	5.3017101945203535
>1k	0.022913569651369325	5.651163665601124
>5k	9.547320688070552E-4	0.7563958261815688
>10k+	0.0014320981032105828	2.190180053219285
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	13298	0.8378994167847043	No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	10697	0.6740118860991113	No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	10407	0.6557391510361271	No Hit
AAAGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	6754	0.42556569867377747	No Hit
AAAGTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	5127	0.3230493540273108	No Hit
AAAGTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	4656	0.2933719119077744	No Hit
AAAGTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4627	0.29154463840147593	No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	4570	0.28795310082013076	No Hit
AAAGTTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	4446	0.2801399313449237	No Hit
AAAGTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	3933	0.2478160931128171	No Hit
AAAGTTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCA	3757	0.23672643321252326	No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	2679	0.16880226632322326	No Hit
AAAGTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	2606	0.16420257784185138	No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	2465	0.15531824803536592	No Hit
AAAGTTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	2369	0.14926934263520564	No Hit
AAAGTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA	2022	0.12740506999087622	No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAGTTGGGA	1967	0.12393955127203439	No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAA	1963	0.12368751354702773	No Hit
AAAGTTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	1891	0.1191508344969075	No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTG	1773	0.11171572160921046	No Hit
AAAGTTGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCT	1731	0.10906932549664035	No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGC	1700	0.10711603312783859	No Hit
AAAGTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	1698	0.10699001426533523	No Hit
AAAGTTGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTC	1622	0.10220129749020834	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	4.4106601876168827E-4	0.0	0.0	0.0	0.0
8	0.0017012546437950834	0.0	0.0	0.0	0.0
9	0.003780565875100185	0.0	0.0	0.0	0.0
10	0.03849876249477022	0.0	0.0	0.0	0.0
11	0.0860078736585292	0.0	0.0	0.0	0.0
12	0.11864675904689415	0.0	0.0	0.0	0.0
13	0.13509222060357995	0.0	0.0	0.0	0.0
14	0.15494019144785592	0.0	0.0	0.0	0.0
15	0.1776865961297087	0.0	0.0	0.0	0.0
16	0.20320541578663495	0.0	0.0	0.0	0.0
17	0.2218562074371292	0.0	0.0	0.0	0.0
18	0.23987690477510673	0.0	0.0	0.0	0.0
19	0.2643245641007546	0.0	0.0	0.0	0.0
20	0.286377865038839	0.0	0.0	0.0	0.0
21	0.30824213768316844	0.0	0.0	0.0	0.0
22	0.33300484416507464	0.0	0.0	0.0	0.0
23	0.3628713145783661	0.0	0.0	0.0	0.0
24	0.3928007944229092	0.0	0.0	0.0	0.0
25	0.4212180479174123	0.0	0.0	0.0	0.0
26	0.45228169752448544	0.0	0.0	0.0	0.0
27	0.48240020566278363	0.0	0.0	0.0	0.0
28	0.5239864302888856	0.0	0.0	0.0	0.0
29	0.5488121462020435	0.0	0.0	0.0	0.0
30	0.5774814374215532	0.0	0.0	0.0	0.0
31	0.6023071533347112	0.0	0.0	0.0	0.0
32	0.6262507372103456	0.0	0.0	0.0	0.0
33	0.6484300570109334	0.0	0.0	0.0	0.0
34	0.674452952117873	0.0	0.0	0.0	0.0
35	0.7132037523376499	0.0	0.0	0.0	0.0
36	0.7426291567321797	0.0	0.0	0.0	0.0
37	0.770605344207921	0.0	0.0	0.0	0.0
38	0.8001567674649541	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAACG	20	7.8573875E-4	44.000004	27
CGACAAT	20	7.8573875E-4	44.000004	30
ACCGTAC	20	7.8573875E-4	44.000004	44
CGAACAA	35	1.4465513E-7	44.0	18
AATTTCG	75	0.0	44.0	42
TCCGATT	25	4.4436092E-5	44.0	19
ACCGATT	35	1.4465513E-7	44.0	35
CGCGATT	25	4.4436092E-5	44.0	15
TTATCGT	30	2.5283298E-6	44.0	39
AATTGCG	70	0.0	44.0	43
CTACGCG	25	4.4436092E-5	44.0	40
ATTATCG	30	2.5283298E-6	44.0	38
CCAACGG	25	4.4436092E-5	44.0	32
GGCGATA	25	4.4436092E-5	44.0	20
TATACGG	25	4.4436092E-5	44.0	34
TATCGTT	35	1.4465513E-7	44.0	40
CGTCAAA	25	4.4436092E-5	44.0	34
TCGGTTA	50	2.7284841E-11	44.0	19
TACGTCG	50	2.7284841E-11	44.0	44
GTACGGT	30	2.5283298E-6	44.0	12
>>END_MODULE