FastQCFastQC Report
Fri 17 Jun 2016
SRR1527521_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527521_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1290773
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA119210.9235551099999767No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC86620.6710707459793472No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT84810.6570481409202082No Hit
AAAGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC55890.43299635179849594No Hit
AAAGTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC42550.3296474283239578No Hit
AAAGTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT38750.30020770499537874No Hit
AAAGTTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC36720.2844806949014273No Hit
AAAGTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT36620.2837059653401489No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC36490.282698816910487No Hit
AAAGTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG32460.2514772155909676No Hit
AAAGTTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG31760.24605410866201882No Hit
AAAGTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC23330.18074440664624997No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC20990.1626157349123355No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT20030.15517833112406287No Hit
AAAGTTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT19340.14983269715124192No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAGTTGGGAAG17450.13519030844308025No Hit
AAAGTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA15930.1234144191116486No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAA15880.12302705433100941No Hit
AAAGTTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT15280.11837867696333902No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCT15140.11729405557754927No Hit
AAAGTTGGGGGCGGGCAGTGGTGGCGCACACCTTTAATCCCAGAACTTGGGA15030.11644185306014304No Hit
AAAGTTGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCT14620.1132654618589016No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTC14550.11272315116600672No Hit
AAAGTTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT14050.10884950335961474No Hit
AAAGTTGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTT13880.10753246310544148No Hit
AAAGTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT13870.10745499014931365No Hit
AAAGTTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT13010.10079231592231941No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGAA206.311744E-446.019
AATTTCG600.046.042
CGTCTAC206.311744E-446.040
AACGCCC253.4173907E-546.039
CGAATAG301.8615829E-646.019
ACTAACG206.311744E-446.042
TCGTACA206.311744E-446.044
GATTCGT253.4173907E-546.09
TCGGTTA301.8615829E-646.019
TCGCGTT206.311744E-446.016
TACGTCG206.311744E-446.044
GCCGAAT351.0197073E-745.99999633
TTGGGAC76600.042.5770235
ATTGCGA601.8189894E-1242.16666844
TTGGGAT134750.042.0230065
CGAACGT554.7293724E-1141.8181838
GCTATCG554.7293724E-1141.8181838
TTAGTAC508.712959E-1041.436
TTGGGGC141150.041.339715
TGGGATA20200.041.331686
Produced by FastQC (version 0.11.5)