##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527521_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1290773
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.612278843762613	31.0	31.0	33.0	30.0	34.0
2	32.14232324351377	33.0	31.0	34.0	31.0	34.0
3	32.706927554264	34.0	31.0	34.0	31.0	34.0
4	36.41612739033122	37.0	37.0	37.0	35.0	37.0
5	35.46090211059575	35.0	35.0	37.0	35.0	37.0
6	35.997901257618494	37.0	35.0	37.0	35.0	37.0
7	36.5174666653238	37.0	37.0	37.0	35.0	37.0
8	36.716007384722175	37.0	37.0	37.0	35.0	37.0
9	38.71614683604321	39.0	39.0	39.0	39.0	39.0
10	37.674184384086125	39.0	37.0	39.0	35.0	39.0
11	37.7599818093499	39.0	37.0	39.0	35.0	39.0
12	37.55955384874025	39.0	37.0	39.0	35.0	39.0
13	37.49765992936016	39.0	37.0	39.0	35.0	39.0
14	38.16955421286315	40.0	38.0	40.0	35.0	40.0
15	38.29544466765264	40.0	38.0	40.0	35.0	40.0
16	38.36027016369261	40.0	38.0	40.0	35.0	40.0
17	38.35043497191218	40.0	38.0	40.0	35.0	40.0
18	38.2356564632201	40.0	38.0	40.0	35.0	40.0
19	38.12777924545989	40.0	38.0	40.0	34.0	40.0
20	38.070345444164076	40.0	38.0	40.0	34.0	40.0
21	38.05610823901647	40.0	38.0	40.0	34.0	40.0
22	38.0567016818604	40.0	38.0	40.0	34.0	40.0
23	37.987139489282775	40.0	38.0	40.0	34.0	40.0
24	37.90329438251342	40.0	37.0	40.0	34.0	40.0
25	37.88062347136174	40.0	37.0	40.0	34.0	40.0
26	37.798925140206684	40.0	37.0	40.0	34.0	40.0
27	37.729609311629545	40.0	37.0	40.0	34.0	40.0
28	37.62621700329957	40.0	36.0	40.0	34.0	40.0
29	37.525478918446545	40.0	36.0	40.0	34.0	40.0
30	37.38834481353422	39.0	36.0	40.0	33.0	40.0
31	37.290915598637405	39.0	35.0	40.0	33.0	40.0
32	37.19755371393731	39.0	35.0	40.0	33.0	40.0
33	36.99809339054969	39.0	35.0	40.0	33.0	40.0
34	36.58704357776309	39.0	35.0	40.0	31.0	40.0
35	36.4392027103139	39.0	35.0	40.0	31.0	40.0
36	36.33478543477435	38.0	35.0	40.0	31.0	40.0
37	36.123099104180206	38.0	35.0	40.0	30.0	40.0
38	36.219530467402095	39.0	35.0	40.0	31.0	40.0
39	36.10412210357669	39.0	35.0	40.0	31.0	40.0
40	35.89202903996288	39.0	35.0	40.0	30.0	40.0
41	35.73629910139118	38.0	35.0	40.0	30.0	40.0
42	35.636121920740514	38.0	35.0	40.0	30.0	40.0
43	35.57914056150849	38.0	35.0	40.0	29.0	40.0
44	35.435621135552104	38.0	35.0	40.0	29.0	40.0
45	35.25716760421856	38.0	34.0	40.0	28.0	40.0
46	35.14532841948197	38.0	34.0	40.0	28.0	40.0
47	34.92942446115622	37.0	34.0	40.0	27.0	40.0
48	34.718010060638086	37.0	34.0	40.0	26.0	40.0
49	34.62736127886158	37.0	34.0	40.0	27.0	40.0
50	34.933530527830996	37.0	34.0	40.0	28.0	40.0
51	34.966296939895706	37.0	35.0	40.0	28.0	40.0
52	34.655681517974116	36.0	34.0	40.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	4.0
12	7.0
13	9.0
14	13.0
15	23.0
16	29.0
17	98.0
18	203.0
19	387.0
20	598.0
21	1059.0
22	1668.0
23	2641.0
24	3978.0
25	5722.0
26	8475.0
27	11549.0
28	15198.0
29	16902.0
30	18543.0
31	24123.0
32	33361.0
33	45281.0
34	95693.0
35	88121.0
36	131088.0
37	225720.0
38	465536.0
39	94743.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.57877953753294	0.234355692286715	0.15579811477308558	0.031066655407263707
8	99.33807106284374	0.5137231720837049	0.09196039892374569	0.0562453661488116
9	96.90170153853543	1.547212406829086	0.5910411822992889	0.9600448723361892
10	52.833147269117035	34.07330336162904	4.418824998663592	8.674724370590337
11	34.342754303041666	24.931881903324594	23.007608619021315	17.717755174612424
12	33.87280335117019	18.39176989292463	24.98905694494694	22.74636981095824
13	26.19624054733094	18.507282070511234	28.67886142644756	26.61761595571026
14	26.291222391543673	20.066502785540138	29.391612622823686	24.250662200092503
15	26.758849154731312	19.83439380898113	27.996324682961294	25.41043235332626
16	30.7048567021467	22.754814363176173	25.363716160781173	21.176612773895954
17	32.76160874142859	21.875806202949704	22.637985145335392	22.724599910286315
18	30.23668762826616	22.372020486948518	23.960990817130508	23.430301067654806
19	28.747579938532958	23.621891688158954	24.519028520119342	23.11149985318875
20	27.465634933485596	26.19624054733094	22.940284620146222	23.397839899037244
21	28.16606792983739	25.083651424379035	26.577020126699274	20.1732605190843
22	27.22221490533192	24.810171889247762	25.305456497773037	22.662156707647277
23	30.081199405317587	24.450310008033945	24.93715006434129	20.531340522307175
24	30.613128722091336	24.567216698830855	22.910999842729897	21.90865473634791
25	29.487756561378337	21.984810652221576	23.3876909417845	25.139741844615592
26	26.644421598530492	22.332974117060086	24.885243183735636	26.137361100673782
27	25.981950350681338	22.23427357095322	24.563962834673486	27.21981324369196
28	29.174533399753482	24.570083198207584	21.707225050415527	24.548158351623407
29	30.05028769582258	24.92057085172993	21.831026834307814	23.198114618139673
30	30.48630549291006	23.549067109398784	23.01489107689733	22.949736320793818
31	31.45603448476223	23.32021199699715	22.907436086748017	22.316317431492603
32	32.9311195694363	22.16787924755166	23.151088533770075	21.749912649241963
33	32.89253803728464	22.506203646961936	23.440372551951427	21.160885763802
34	28.955439879823953	24.84464735472465	26.185239387560788	20.014673377890613
35	27.798768644835302	28.525774865138953	24.593015193221426	19.082441296804316
36	28.553200291608206	27.973160269079074	22.6101723540855	20.863467085227224
37	28.061556912020936	30.136127731212227	21.582958428786473	20.219356927980364
38	30.02247490457269	27.670008591750832	21.46070610401674	20.846810399659738
39	29.03577933532852	25.69561030483284	20.94039773066217	24.32821262917647
40	28.338445257221835	25.896962517809097	22.07351718698795	23.691075037981115
41	25.32544452045402	26.434082522643408	21.774316630422234	26.466156326480334
42	24.244541836558405	25.48643332328767	23.573780982403566	26.695243857750356
43	24.858747432739918	24.479129947713503	23.850049543955443	26.812073075591137
44	26.402551029499378	23.180063419361886	23.071523807826782	27.345861743311957
45	26.130620953490656	24.666614501542874	23.239020338975173	25.963744205991297
46	26.273093719809758	25.656796353812794	22.891399184829556	25.178710741547896
47	26.100329027644676	25.31351368521034	23.84416159928973	24.741995687855262
48	25.68553882053622	25.587458058078376	23.969048004567806	24.757955116817598
49	27.686045493669297	23.986324473784315	23.511027887940017	24.816602144606374
50	25.735353931326422	24.520113141505128	23.40365037074683	26.34088255642162
51	23.660938058047385	25.901455949264513	23.869417782987405	26.5681882097007
52	24.424124148862735	25.39501523505682	25.28066515181213	24.90019546426831
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	37.5
2	75.0
3	52.5
4	30.0
5	39.0
6	48.0
7	845.0
8	1642.0
9	1991.5
10	2341.0
11	2939.0
12	3537.0
13	2964.0
14	2555.5
15	2720.0
16	2867.0
17	3014.0
18	3262.5
19	3511.0
20	4597.5
21	5684.0
22	7618.0
23	9552.0
24	10925.0
25	12298.0
26	14324.0
27	16350.0
28	18113.5
29	19877.0
30	21488.0
31	23099.0
32	25618.5
33	28138.0
34	27142.5
35	26147.0
36	29184.0
37	32221.0
38	34547.5
39	37450.5
40	38027.0
41	41392.5
42	44758.0
43	45090.0
44	45422.0
45	50247.0
46	55072.0
47	59534.0
48	63996.0
49	67753.5
50	71511.0
51	78234.5
52	84958.0
53	95343.0
54	105728.0
55	107036.5
56	108345.0
57	104634.5
58	100924.0
59	100997.0
60	101070.0
61	87906.0
62	74742.0
63	64105.5
64	41965.0
65	30461.0
66	26341.5
67	22222.0
68	22559.5
69	22897.0
70	21651.5
71	20406.0
72	15821.0
73	11236.0
74	7426.5
75	3617.0
76	2435.5
77	1254.0
78	921.5
79	589.0
80	394.0
81	199.0
82	196.0
83	193.0
84	155.5
85	118.0
86	63.0
87	8.0
88	4.5
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1290773.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.341214763010786
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.27840394974835	8.796620911084807
2	9.187328609341124	2.267655909324641
3	3.3429074909998002	1.2376661783791825
4	1.6845955689215437	0.8315982281950846
5	1.0098437921012375	0.6231349557707295
6	0.7420008437463197	0.5494315060205125
7	0.5513050373952411	0.47626417064970533
8	0.48191840074958364	0.47579667855178487
9	0.42436329433235553	0.47134426976049215
>10	7.886320763922623	25.42297012436119
>50	2.1579744712875604	18.24332721577931
>100	1.178624864651213	28.738540309323664
>500	0.049188196598605995	4.014960871179115
>1k	0.022702244583971996	5.153787412262758
>5k	0.001891853715331	1.769138586203331
>10k+	6.306179051103333E-4	0.9277626731537
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	11921	0.9235551099999767	No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	8662	0.6710707459793472	No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	8481	0.6570481409202082	No Hit
AAAGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5589	0.43299635179849594	No Hit
AAAGTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4255	0.3296474283239578	No Hit
AAAGTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	3875	0.30020770499537874	No Hit
AAAGTTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	3672	0.2844806949014273	No Hit
AAAGTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	3662	0.2837059653401489	No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	3649	0.282698816910487	No Hit
AAAGTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	3246	0.2514772155909676	No Hit
AAAGTTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	3176	0.24605410866201882	No Hit
AAAGTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2333	0.18074440664624997	No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	2099	0.1626157349123355	No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	2003	0.15517833112406287	No Hit
AAAGTTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	1934	0.14983269715124192	No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAGTTGGGAAG	1745	0.13519030844308025	No Hit
AAAGTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	1593	0.1234144191116486	No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAA	1588	0.12302705433100941	No Hit
AAAGTTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	1528	0.11837867696333902	No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCT	1514	0.11729405557754927	No Hit
AAAGTTGGGGGCGGGCAGTGGTGGCGCACACCTTTAATCCCAGAACTTGGGA	1503	0.11644185306014304	No Hit
AAAGTTGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCT	1462	0.1132654618589016	No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTC	1455	0.11272315116600672	No Hit
AAAGTTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCAT	1405	0.10884950335961474	No Hit
AAAGTTGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTT	1388	0.10753246310544148	No Hit
AAAGTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1387	0.10745499014931365	No Hit
AAAGTTGGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCAT	1301	0.10079231592231941	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	7.747295612783967E-5	0.0	0.0	0.0	0.0
8	0.0013945132103011142	0.0	0.0	0.0	0.0
9	0.003796174850264144	0.0	0.0	0.0	0.0
10	0.042455179958056144	0.0	0.0	0.0	0.0
11	0.09405216873919736	0.0	0.0	0.0	0.0
12	0.12504135119033324	0.0	0.0	0.0	0.0
13	0.14301507701199204	0.0	0.0	0.0	0.0
14	0.16300309969297466	0.0	0.0	0.0	0.0
15	0.18732960791711634	0.0	0.0	0.0	0.0
16	0.21490998029862726	0.0	0.0	0.0	0.0
17	0.23404580046220366	0.0	0.0	0.0	0.0
18	0.2520195262838625	0.0	0.0	0.0	0.0
19	0.27642350746413197	0.0	0.0	0.0	0.0
20	0.30020770499537874	0.0	0.0	0.0	0.0
21	0.32422432139500906	0.0	0.0	0.0	0.0
22	0.3489381943997899	0.0	0.0	0.0	0.0
23	0.37489163470261616	0.0	0.0	0.0	0.0
24	0.4007676020493146	0.0	0.0	0.0	0.0
25	0.4223825568089819	0.0	0.0	0.0	0.0
26	0.44570191660346165	0.0	0.0	0.0	0.0
27	0.4647602638109102	0.0	0.0	0.0	0.0
28	0.49745385129685854	0.0	0.0	0.0	0.0
29	0.5186814412758867	0.0	0.0	0.0	0.0
30	0.5406837608161931	0.0	0.0	0.0	0.0
31	0.5584250677694683	0.0	0.0	0.0	0.0
32	0.5772509961085334	0.0	0.0	0.0	0.0
33	0.5936752628076354	0.0	0.0	0.0	0.0
34	0.6155226364356862	0.0	0.0	0.0	0.0
35	0.6516250339912595	0.0	0.0	0.0	0.0
36	0.6777334202063414	0.0	0.0	0.0	0.0
37	0.703067076860145	0.0	0.0	0.0	0.0
38	0.7264639096107526	0.0	0.0	0.0	0.0
39	0.750403053054255	0.0	0.0	0.0	0.0
40	0.7740323046732462	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAA	20	6.311744E-4	46.0	19
AATTTCG	60	0.0	46.0	42
CGTCTAC	20	6.311744E-4	46.0	40
AACGCCC	25	3.4173907E-5	46.0	39
CGAATAG	30	1.8615829E-6	46.0	19
ACTAACG	20	6.311744E-4	46.0	42
TCGTACA	20	6.311744E-4	46.0	44
GATTCGT	25	3.4173907E-5	46.0	9
TCGGTTA	30	1.8615829E-6	46.0	19
TCGCGTT	20	6.311744E-4	46.0	16
TACGTCG	20	6.311744E-4	46.0	44
GCCGAAT	35	1.0197073E-7	45.999996	33
TTGGGAC	7660	0.0	42.577023	5
ATTGCGA	60	1.8189894E-12	42.166668	44
TTGGGAT	13475	0.0	42.023006	5
CGAACGT	55	4.7293724E-11	41.81818	38
GCTATCG	55	4.7293724E-11	41.81818	38
TTAGTAC	50	8.712959E-10	41.4	36
TTGGGGC	14115	0.0	41.33971	5
TGGGATA	2020	0.0	41.33168	6
>>END_MODULE