FastQCFastQC Report
Fri 17 Jun 2016
SRR1527520_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527520_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1738886
Sequences flagged as poor quality0
Sequence length52
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA154650.8893624998993609No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT113410.6521991665928646No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC112330.6459882936546731No Hit
AAAGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC72190.4151508494518905No Hit
AAAGTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC59770.34372581066268865No Hit
AAAGTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT53310.3065755891990619No Hit
AAAGTTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC52680.30295257998511693No Hit
AAAGTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT51470.29599410197103204No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC50240.2889206077914251No Hit
AAAGTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG45120.2594764694177767No Hit
AAAGTTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG40720.23417291300292256No Hit
AAAGTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC30690.1764923059936074No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT27890.16039004282051844No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAGTTGGGAAG27660.15906735691701468No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC26840.15435169413061006No Hit
AAAGTTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT25220.14503538472332286No Hit
AAAGTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA21700.12479253959143959No Hit
AAAGTTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT20300.11674140800489509No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAA20130.11576377059795753No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTC19150.1101279784873764No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCT19150.1101279784873764No Hit
AAAGTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT19140.11007047040461537No Hit
AAAGTTGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTT19000.10926535724596093No Hit
AAAGTTGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCT18510.10644746119067033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACAA351.0199801E-746.00000418
ACGCAAT351.0199801E-746.00000432
AAATCGC206.312353E-446.016
CGAGTTA206.312353E-446.033
ATAGCGG206.312353E-446.036
CACGACG253.4178844E-546.023
TAACGCG206.312353E-446.020
CGTTCGA206.312353E-446.041
TCGATAA206.312353E-446.044
CGTACGT206.312353E-446.022
TTACGCC301.8619576E-646.025
ATACGCC301.8619576E-646.033
GGTTATA453.110472E-1046.09
CGATAGT206.312353E-446.013
TACGTCG405.6134013E-946.044
TACCGAT301.8619576E-646.045
TTGGGAC97900.042.734425
TTGGGAT177800.042.5719955
CGCATTA601.8189894E-1242.16666833
TGGGATT63650.041.916736
Produced by FastQC (version 0.11.5)