##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527520_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1738886
Sequences flagged as poor quality	0
Sequence length	52
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.850422051819383	31.0	30.0	31.0	26.0	33.0
2	30.710246675170197	31.0	31.0	33.0	28.0	34.0
3	31.728911498511117	31.0	31.0	34.0	30.0	34.0
4	35.77603534676799	37.0	35.0	37.0	35.0	37.0
5	34.743911906818504	35.0	35.0	37.0	32.0	37.0
6	35.438951719664196	35.0	35.0	37.0	35.0	37.0
7	36.131014914146185	37.0	35.0	37.0	35.0	37.0
8	36.5095998242553	37.0	37.0	37.0	35.0	37.0
9	38.57945086681933	39.0	39.0	39.0	37.0	39.0
10	37.062710839008425	39.0	37.0	39.0	33.0	39.0
11	37.08401873383304	39.0	37.0	39.0	34.0	39.0
12	36.89657976428587	39.0	35.0	39.0	33.0	39.0
13	36.81250064696593	39.0	35.0	39.0	33.0	39.0
14	37.475565390715666	40.0	37.0	40.0	33.0	40.0
15	37.6040861793125	40.0	37.0	40.0	33.0	40.0
16	37.62850871189946	40.0	37.0	40.0	33.0	40.0
17	37.66310327416518	40.0	37.0	40.0	33.0	40.0
18	37.57293462596168	40.0	36.0	40.0	33.0	40.0
19	37.42341476094465	39.0	36.0	40.0	33.0	40.0
20	37.314400138939526	39.0	36.0	40.0	32.0	40.0
21	37.37707474785581	39.0	36.0	40.0	32.0	40.0
22	37.3965573361336	39.0	36.0	40.0	33.0	40.0
23	37.377518710254726	39.0	36.0	40.0	33.0	40.0
24	37.32095548529346	39.0	36.0	40.0	33.0	40.0
25	37.29033990727397	39.0	36.0	40.0	33.0	40.0
26	37.163332156334576	39.0	35.0	40.0	33.0	40.0
27	37.08288237411768	39.0	35.0	40.0	33.0	40.0
28	36.90847531120499	39.0	35.0	40.0	32.0	40.0
29	36.86659217453013	39.0	35.0	40.0	32.0	40.0
30	36.78111331047579	39.0	35.0	40.0	32.0	40.0
31	36.61226152835781	38.0	35.0	40.0	31.0	40.0
32	36.55385804474819	38.0	35.0	40.0	31.0	40.0
33	36.38238389405631	38.0	35.0	40.0	31.0	40.0
34	36.02187262419733	38.0	35.0	40.0	30.0	40.0
35	35.85792168089225	38.0	35.0	40.0	30.0	40.0
36	35.82434386152974	38.0	34.0	40.0	30.0	40.0
37	35.69559591600599	38.0	34.0	40.0	30.0	40.0
38	35.582552277722634	38.0	34.0	40.0	29.0	40.0
39	35.400306863129614	38.0	34.0	40.0	29.0	40.0
40	35.58075284981304	38.0	35.0	40.0	29.0	40.0
41	35.5981979267186	38.0	35.0	40.0	30.0	40.0
42	35.631642902409936	38.0	35.0	40.0	30.0	40.0
43	35.58452480496134	38.0	35.0	40.0	30.0	40.0
44	35.470447746430764	38.0	35.0	40.0	30.0	40.0
45	35.31366173515688	38.0	34.0	40.0	29.0	40.0
46	35.11747693638341	37.0	34.0	40.0	29.0	40.0
47	34.99753635373452	37.0	34.0	40.0	28.0	40.0
48	34.877838455194876	37.0	34.0	40.0	28.0	40.0
49	34.75330412689504	36.0	34.0	40.0	27.0	40.0
50	34.59032046954199	36.0	34.0	40.0	27.0	40.0
51	34.399692101724895	36.0	34.0	40.0	27.0	40.0
52	33.95845846133674	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	5.0
13	11.0
14	16.0
15	33.0
16	72.0
17	173.0
18	323.0
19	654.0
20	1088.0
21	1901.0
22	2997.0
23	4458.0
24	6695.0
25	9768.0
26	14000.0
27	19459.0
28	24884.0
29	29351.0
30	34638.0
31	43514.0
32	60009.0
33	86380.0
34	146713.0
35	133963.0
36	210147.0
37	335055.0
38	522002.0
39	50576.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	0.0	100.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.61205047369408	0.22175116712653964	0.1431376179922088	0.023060741187173855
8	99.35797976405584	0.5029656918279864	0.08810238278990111	0.050952161326274406
9	96.89157311060069	1.5416766826577475	0.5926207928524354	0.9741294138891221
10	52.42247047822571	34.47655567990081	4.28107420497951	8.819899636893966
11	34.01166033886063	25.07915987592056	23.04302869768346	17.866151087535354
12	33.48068821072802	18.42369194990356	25.138853265826512	22.956766573541913
13	25.76672651341146	18.408222275640842	28.876878645293598	26.948172565654104
14	25.99031793918635	20.108621266719037	29.403997731881216	24.497063062213396
15	26.57356491454874	19.77260153914633	28.01661523527132	25.637218311033617
16	30.675271409396593	22.633341116093867	25.46515412741261	21.226233347096933
17	32.55636079650995	21.788259839920503	22.72098343422168	22.93439592934787
18	30.062235247163986	22.310605755638953	23.902026929884997	23.72513206731206
19	28.632181753145403	23.529202029345225	24.590053632037982	23.24856258547139
20	27.236805633031725	26.208676129429993	22.960274566590332	23.59424367094795
21	28.032659990361648	24.99502555084117	26.629807819489027	20.342506639308155
22	27.118338982544	24.57947214480995	25.33892388575214	22.963264986893908
23	29.87205601747326	24.378308871311862	24.991517557792747	20.758117553422135
24	30.453290209939006	24.592526479596707	22.883788816518162	22.070394493946125
25	29.277479949806946	21.926221730464217	23.410562854609214	25.385735465119623
26	26.65459380315903	22.243723855387877	24.758954871107132	26.342727470345956
27	25.874036595843545	22.195129525454803	24.47066685222608	27.460167026475574
28	29.196796109693217	24.552500853995028	21.68117978981946	24.569523246492295
29	30.024222404458946	24.870348027415254	21.838809444667447	23.266620123458353
30	30.369903489935513	23.4331635311343	23.030491935641553	23.166441043288636
31	31.39872309053037	23.325163351709083	22.860613059165466	22.41550049859508
32	32.81710244374847	22.113755588347942	23.210607250849108	21.858534717054482
33	32.87432298609569	22.429992535450857	23.447195503327993	21.248488975125454
34	28.93611197053746	24.859248967442372	26.0163691006771	20.188269961343067
35	27.6065250971024	28.557421245556064	24.612309260066503	19.223744397275038
36	28.525389243458164	27.895388196811062	22.52988407520677	21.049338484524
37	27.83839768679488	30.08006275281991	21.764509001740194	20.317030558645016
38	30.01875913659665	27.60940050124045	21.495371174418565	20.876469187744338
39	28.812297068352954	25.73429195473424	20.909996399994018	24.54341457691879
40	28.19575291307193	25.915384907348727	22.14147448423876	23.74738769534058
41	25.025504834704513	26.52508560078119	21.735927484607963	26.71348207990633
42	23.98207818108835	25.505812341924656	23.62696577003898	26.88514370694801
43	24.616794890521863	24.57366382845109	23.930780971265513	26.878760309761535
44	26.396612543893045	23.072932900719195	23.128600724831873	27.401853830555883
45	26.079858024045276	24.620130359322005	23.349374254551478	25.950637362081242
46	26.24473369732116	25.64900746799963	22.931808065623624	25.17445076905559
47	26.006535218524963	25.324719389310168	23.825771212143867	24.842974180021002
48	25.69696920902233	25.622323717598512	23.828301567785353	24.85240550559381
49	27.565119277514455	23.989554231847286	23.52667167370374	24.91865481693452
50	25.655563389434384	24.592756511927753	23.33396208837152	26.417718010266345
51	23.509246724627147	25.868975884560573	23.89616110544337	26.725616285368908
52	24.3381107214619	25.444106169122072	25.207057851981098	25.010725257434935
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	38.0
2	76.0
3	56.5
4	37.0
5	58.0
6	79.0
7	1057.5
8	2036.0
9	2492.5
10	2949.0
11	3662.5
12	4376.0
13	3874.5
14	3504.5
15	3636.0
16	4000.5
17	4365.0
18	4704.0
19	5043.0
20	6647.0
21	8251.0
22	10727.0
23	13203.0
24	15125.5
25	17048.0
26	19417.5
27	21787.0
28	23776.0
29	25765.0
30	28021.0
31	30277.0
32	33546.5
33	36816.0
34	35952.5
35	35089.0
36	39081.0
37	43073.0
38	46349.5
39	50459.5
40	51293.0
41	55925.5
42	60558.0
43	61287.0
44	62016.0
45	68441.0
46	74866.0
47	81459.0
48	88052.0
49	92495.0
50	96938.0
51	106029.0
52	115120.0
53	128242.5
54	141365.0
55	144072.5
56	146780.0
57	140763.5
58	134747.0
59	134973.0
60	135199.0
61	117554.0
62	99909.0
63	86022.5
64	56719.5
65	41303.0
66	35454.0
67	29605.0
68	30430.5
69	31256.0
70	29591.5
71	27927.0
72	21478.0
73	15029.0
74	9976.0
75	4923.0
76	3326.5
77	1730.0
78	1275.5
79	821.0
80	533.0
81	245.0
82	172.0
83	99.0
84	71.0
85	43.0
86	26.5
87	10.0
88	7.0
89	5.5
90	7.0
91	3.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1738886.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	11.970460278213856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.69240357492366	8.821319897997284
2	9.159439288999499	2.1928540835935975
3	3.4174825105374245	1.2272651593163642
4	1.6613201269861348	0.7954706635793892
5	0.9404837459148883	0.5629011661389972
6	0.6396128986689014	0.4593876478169585
7	0.48440523761894516	0.4058987558833429
8	0.37344192663556297	0.3576217399208528
9	0.3193178479175121	0.34401434531591807
>10	5.4818928564235705	17.63888970392606
>50	2.373231392579741	19.856157515306265
>100	1.3438200074317832	31.607860922942514
>500	0.0837781015023756	6.54448429713552
>1k	0.025037133782319147	5.035810324121057
>5k	0.002888900051806055	1.9576566384248661
>10k+	0.0014444500259030275	2.192407138581036
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAAGTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	15465	0.8893624998993609	No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	11341	0.6521991665928646	No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	11233	0.6459882936546731	No Hit
AAAGTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	7219	0.4151508494518905	No Hit
AAAGTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	5977	0.34372581066268865	No Hit
AAAGTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	5331	0.3065755891990619	No Hit
AAAGTTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	5268	0.30295257998511693	No Hit
AAAGTTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	5147	0.29599410197103204	No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	5024	0.2889206077914251	No Hit
AAAGTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4512	0.2594764694177767	No Hit
AAAGTTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAG	4072	0.23417291300292256	No Hit
AAAGTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	3069	0.1764923059936074	No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	2789	0.16039004282051844	No Hit
AAAGTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTAAAGTTGGGAAG	2766	0.15906735691701468	No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	2684	0.15435169413061006	No Hit
AAAGTTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2522	0.14503538472332286	No Hit
AAAGTTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA	2170	0.12479253959143959	No Hit
AAAGTTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCTCT	2030	0.11674140800489509	No Hit
AAAGTTGGGGAGTGCAGTGCTAAAGTTGGGAAAAAAAAAAAAAAAAAAAAAA	2013	0.11576377059795753	No Hit
AAAGTTGGGGGGCTGGTGAGATGGCTCAGCGGGTAAGAGCACCCGACTGCTC	1915	0.1101279784873764	No Hit
AAAGTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGGCTGCT	1915	0.1101279784873764	No Hit
AAAGTTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	1914	0.11007047040461537	No Hit
AAAGTTGGGGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGCTCTT	1900	0.10926535724596093	No Hit
AAAGTTGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCT	1851	0.10644746119067033	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	3.450484965661924E-4	0.0	0.0	0.0	0.0
8	0.0016102263173088977	0.0	0.0	0.0	0.0
9	0.0032779607173788277	0.0	0.0	0.0	0.0
10	0.033872260746247884	0.0	0.0	0.0	0.0
11	0.07619820965836749	0.0	0.0	0.0	0.0
12	0.10518228336992765	0.0	0.0	0.0	0.0
13	0.12134205462577766	0.0	0.0	0.0	0.0
14	0.13853697137132623	0.0	0.0	0.0	0.0
15	0.15987247007566913	0.0	0.0	0.0	0.0
16	0.18373832442149743	0.0	0.0	0.0	0.0
17	0.2007032088360019	0.0	0.0	0.0	0.0
18	0.21709301242289603	0.0	0.0	0.0	0.0
19	0.23704831714097416	0.0	0.0	0.0	0.0
20	0.2586138481763612	0.0	0.0	0.0	0.0
21	0.27805158014959	0.0	0.0	0.0	0.0
22	0.29817940911595125	0.0	0.0	0.0	0.0
23	0.3237129978618495	0.0	0.0	0.0	0.0
24	0.3492465866077477	0.0	0.0	0.0	0.0
25	0.3717897550500723	0.0	0.0	0.0	0.0
26	0.392780205257849	0.0	0.0	0.0	0.0
27	0.41515084945189046	0.0	0.0	0.0	0.0
28	0.44810298087396183	0.0	0.0	0.0	0.0
29	0.4672531724333855	0.0	0.0	0.0	0.0
30	0.48881870346877254	0.0	0.0	0.0	0.0
31	0.5063586687108873	0.0	0.0	0.0	0.0
32	0.5238986339530021	0.0	0.0	0.0	0.0
33	0.5406334860364624	0.0	0.0	0.0	0.0
34	0.5598411856786472	0.0	0.0	0.0	0.0
35	0.595553705073248	0.0	0.0	0.0	0.0
36	0.6208572614881022	0.0	0.0	0.0	0.0
37	0.6450106562477356	0.0	0.0	0.0	0.0
38	0.6657135660417072	0.0	0.0	0.0	0.0
39	0.6893493880564914	0.0	0.0	0.0	0.0
40	0.7133302585678417	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACAA	35	1.0199801E-7	46.000004	18
ACGCAAT	35	1.0199801E-7	46.000004	32
AAATCGC	20	6.312353E-4	46.0	16
CGAGTTA	20	6.312353E-4	46.0	33
ATAGCGG	20	6.312353E-4	46.0	36
CACGACG	25	3.4178844E-5	46.0	23
TAACGCG	20	6.312353E-4	46.0	20
CGTTCGA	20	6.312353E-4	46.0	41
TCGATAA	20	6.312353E-4	46.0	44
CGTACGT	20	6.312353E-4	46.0	22
TTACGCC	30	1.8619576E-6	46.0	25
ATACGCC	30	1.8619576E-6	46.0	33
GGTTATA	45	3.110472E-10	46.0	9
CGATAGT	20	6.312353E-4	46.0	13
TACGTCG	40	5.6134013E-9	46.0	44
TACCGAT	30	1.8619576E-6	46.0	45
TTGGGAC	9790	0.0	42.73442	5
TTGGGAT	17780	0.0	42.571995	5
CGCATTA	60	1.8189894E-12	42.166668	33
TGGGATT	6365	0.0	41.91673	6
>>END_MODULE