FastQCFastQC Report
Fri 17 Jun 2016
SRR1527519_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527519_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1499021
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAA74590.4975914280053448No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAA63160.4213416623249441No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATGCTTGGGA58910.39298982469224913No Hit
ATGCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT57230.38178251005156033No Hit
ATGCTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC57070.3807151467524471No Hit
ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG52370.3493613498409962No Hit
ATGCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC52020.34702649262418606No Hit
ATGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC50360.33595259839588637No Hit
ATGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT47980.3200755693215772No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAA42040.2804497068419989No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAA37740.25176431817833106No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAA36970.24662763230134868No Hit
ATGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC35470.23662110137216225No Hit
ATGCTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC35300.2354870278668544No Hit
ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31610.21087096178105577No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAA23290.15536807022716825No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAA22710.15149887826788283No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGAAAAAAAAAAAAAAAA22470.14989783331921303No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAA21330.1422928698130313No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAA19140.1276833346564191No Hit
ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG17980.11994495073784824No Hit
ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAA16630.11093907290158043No Hit
ATGCTTGGGGGGGCTGGAGAGATGGCTCAGTGGTTATGAGCACCGACTGC16150.10773698300424077No Hit
ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTG15120.10086583176619941No Hit
ATGCTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG15110.10079912156000483No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGCAAAAAAAAAAAAAAAA15040.1003321501166428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGTC302.5282334E-644.00000439
AGTACGG302.5282334E-644.00000412
TAATCGT302.5282334E-644.00000430
AACGTTG254.443491E-544.018
AAGTACG254.443491E-544.044
GTACCGG351.4464786E-744.026
ACGTTTA207.85725E-444.010
ACGTTAG207.85725E-444.039
CGATTAC207.85725E-444.044
TACGGAA351.4464786E-744.031
TATGCGC254.443491E-544.040
TACGCAC454.802132E-1044.016
TAATTCG207.85725E-444.043
CGTAGCA207.85725E-444.032
AACGATA454.802132E-1044.044
CATACGT254.443491E-544.030
CGTAATT351.4464786E-744.040
CATAACG254.443491E-544.033
TATAGGG207.85725E-444.013
CATCGCG254.443491E-544.028
Produced by FastQC (version 0.11.5)