##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527519_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1499021
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.647181727274003	31.0	28.0	33.0	25.0	33.0
2	30.508796741339847	31.0	31.0	33.0	26.0	34.0
3	31.53839806113457	33.0	31.0	34.0	28.0	34.0
4	35.804271587923054	37.0	35.0	37.0	33.0	37.0
5	36.0356005686378	37.0	35.0	37.0	35.0	37.0
6	36.35199039906713	37.0	37.0	37.0	35.0	37.0
7	36.433821140597765	37.0	37.0	37.0	35.0	37.0
8	36.551023634758955	37.0	37.0	37.0	35.0	37.0
9	38.521019385318816	39.0	39.0	39.0	37.0	39.0
10	37.23366917474805	39.0	37.0	39.0	34.0	39.0
11	37.072830200510865	39.0	37.0	39.0	34.0	39.0
12	36.86639413323763	39.0	35.0	39.0	33.0	39.0
13	36.68984623964574	39.0	35.0	39.0	33.0	39.0
14	37.52586721600298	39.0	37.0	40.0	33.0	40.0
15	37.63179701952141	40.0	37.0	40.0	33.0	40.0
16	37.786022343916464	40.0	37.0	40.0	33.0	40.0
17	37.76754895361706	40.0	37.0	40.0	34.0	40.0
18	37.579525570355585	40.0	36.0	40.0	33.0	40.0
19	37.51659116183162	39.0	36.0	40.0	33.0	40.0
20	37.3979937572589	39.0	36.0	40.0	33.0	40.0
21	37.365607953457626	39.0	36.0	40.0	33.0	40.0
22	37.159772945142194	39.0	36.0	40.0	33.0	40.0
23	37.015566826615505	38.0	36.0	40.0	33.0	40.0
24	36.78548399255247	38.0	35.0	40.0	32.0	40.0
25	36.37682193911893	38.0	35.0	40.0	32.0	40.0
26	35.645396562156236	37.0	35.0	39.0	31.0	40.0
27	35.349716915240016	37.0	34.0	39.0	30.0	40.0
28	35.18903937970182	36.0	34.0	39.0	30.0	40.0
29	34.79792811441601	36.0	34.0	38.0	29.0	40.0
30	34.40981814130689	36.0	33.0	38.0	27.0	40.0
31	34.26167545351266	35.0	33.0	38.0	27.0	40.0
32	33.90770176001537	35.0	33.0	38.0	26.0	40.0
33	33.29844211655474	35.0	32.0	38.0	24.0	39.0
34	32.70640104441499	35.0	31.0	38.0	22.0	39.0
35	32.40331656461117	35.0	31.0	38.0	21.0	39.0
36	31.92519050767134	34.0	31.0	37.0	21.0	39.0
37	31.372938738016344	34.0	30.0	37.0	18.0	39.0
38	30.631889079605955	34.0	29.0	37.0	15.0	38.0
39	29.584145919236622	33.0	27.0	36.0	10.0	38.0
40	29.12209168517319	33.0	26.0	36.0	10.0	38.0
41	28.578085964105906	32.0	24.0	35.0	10.0	38.0
42	28.135828650832778	32.0	24.0	35.0	9.0	38.0
43	27.61468985424487	31.0	23.0	35.0	9.0	37.0
44	27.180356379263532	31.0	23.0	34.0	9.0	37.0
45	26.69934977562022	30.0	22.0	34.0	9.0	36.0
46	26.584620895904727	30.0	22.0	34.0	9.0	36.0
47	26.20427932630697	30.0	21.0	34.0	9.0	36.0
48	25.733845623243436	29.0	20.0	33.0	8.0	35.0
49	25.08317762059371	28.0	20.0	33.0	8.0	35.0
50	27.113185872646213	31.0	22.0	34.0	8.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	6.0
11	12.0
12	37.0
13	75.0
14	135.0
15	229.0
16	404.0
17	724.0
18	1311.0
19	2210.0
20	3679.0
21	5598.0
22	8231.0
23	11499.0
24	16517.0
25	22777.0
26	31837.0
27	44256.0
28	58865.0
29	70994.0
30	70839.0
31	68320.0
32	83149.0
33	119016.0
34	163423.0
35	292360.0
36	328387.0
37	90985.0
38	3141.0
39	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.42535828383991	0.2758467026145731	0.22648115003058664	0.07231386351492074
8	99.10901848606524	0.5105999182132872	0.21720843136954052	0.16317316435193369
9	97.26354734189849	1.5378036731973734	0.5865161328627151	0.6121328520414324
10	60.1572626400831	29.039686568767216	4.0124854821913765	6.790565308958313
11	35.85293334783168	34.57863498910289	16.288964597560675	13.279467065504752
12	34.948342951833226	26.537253313996267	21.81803990737955	16.696363826790954
13	23.721949192172758	28.21908432236773	25.430197442197272	22.62876904326224
14	24.74374942045508	31.670203419431747	21.638389322097556	21.947657838015612
15	22.513693937576594	32.36218838828809	21.808633768306116	23.315483905829204
16	26.850457732079803	34.99323892060218	20.4915074572004	17.664795890117617
17	30.705440417445786	32.19901522393616	17.91949545736851	19.176048901249548
18	25.968949067424674	32.687600774105235	22.28200939146283	19.06144076700727
19	27.09428353572098	32.39480967911724	19.972435342800402	20.53847144236138
20	21.73538596190447	35.43325944066161	20.245613637167192	22.585740960266733
21	23.884255123844163	34.64034192983287	24.274376409670044	17.201026536652922
22	22.9551820821723	35.70130104915141	20.834731468071492	20.508785400604797
23	23.992258947673182	34.230207582148616	23.90286727137245	17.874666198805752
24	27.040982080971514	33.58598712092759	19.23161850300963	20.141412295091264
25	22.977063029804118	30.807440322717294	21.05374107500829	25.1617555724703
26	24.365035579888474	30.09590926344594	23.97865006560949	21.560405091056094
27	23.824482779093824	29.748816060615564	23.236632442107215	23.1900687181834
28	30.054282094780525	32.5748605256364	18.22896410390515	19.141893275677926
29	32.415423132831364	31.53818392137268	17.411830788227782	18.634562157568173
30	31.909359508639305	32.11509378454338	16.98555257064444	18.989994136172875
31	32.45885147706403	31.717033984180343	17.376607799357046	18.44750673939858
32	31.499225160955053	32.98406093043393	18.54163484033913	16.975079068271892
33	31.10736940976811	34.871692924915656	16.559341063267293	17.46159660204894
34	25.263288506298444	37.40421248268036	18.940161612145527	18.392337398875664
35	24.28218150379481	39.846273000845216	19.576643689447977	16.294901805911994
36	25.547007013243977	39.41545848924065	16.656337703074207	18.38119679444117
37	25.45608100220077	39.148284113431366	17.60975997000709	17.78587491436077
38	28.507405833540687	36.733174518569115	16.717711092773214	18.041708555116973
39	26.755195557633947	35.17542449371957	17.273673951198816	20.795705997447666
40	26.929442616214182	33.873441399420024	18.59807167477974	20.599044309586056
41	26.037994130836058	33.3940618577058	19.165041717227442	21.402902294230703
42	25.667685776249964	31.902354936988875	20.073968276628545	22.355991010132612
43	26.560268335133397	30.662545754862673	20.420861348840344	22.35632456116359
44	25.739732798940107	30.1148549620052	19.888847454438597	24.256564784616092
45	26.640854264216447	30.72051692404576	20.366625951204153	22.27200286053364
46	26.141928632087208	32.100884510623935	20.816319451161792	20.940867406127065
47	26.63845269679344	30.99209417346388	19.996851278267616	22.372601851475064
48	26.718705074845516	30.597036332379602	20.313658047485657	22.370600545289225
49	26.545391959152003	30.767414199000548	20.516924045760533	22.170269796086913
50	24.719867166637425	29.683773609575848	21.76133623211416	23.835022991672563
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	41.0
4	82.0
5	62.5
6	43.0
7	69.5
8	96.0
9	894.0
10	1692.0
11	3194.0
12	4696.0
13	4356.0
14	4016.0
15	3957.5
16	3899.0
17	4880.0
18	5861.0
19	7501.0
20	9141.0
21	12141.5
22	15142.0
23	19292.5
24	23443.0
25	29073.5
26	34704.0
27	38595.0
28	42486.0
29	44312.0
30	46138.0
31	45894.5
32	45651.0
33	48638.0
34	51625.0
35	56364.0
36	61103.0
37	59573.5
38	58044.0
39	56770.0
40	55496.0
41	53390.5
42	51285.0
43	49130.5
44	46976.0
45	50002.5
46	53029.0
47	55194.5
48	57360.0
49	58606.5
50	59853.0
51	65828.5
52	71804.0
53	78585.5
54	85367.0
55	96129.0
56	106891.0
57	101451.0
58	96011.0
59	95200.0
60	94389.0
61	91877.5
62	89366.0
63	78734.5
64	68103.0
65	58026.0
66	47949.0
67	39881.5
68	31814.0
69	28641.0
70	25468.0
71	21533.5
72	17599.0
73	20112.5
74	22626.0
75	14432.5
76	6239.0
77	4167.5
78	2096.0
79	1640.5
80	1185.0
81	679.0
82	173.0
83	122.0
84	71.0
85	39.0
86	7.0
87	4.0
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1499021.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.425895042629367
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.41152401326993	18.837119217527327
2	7.977753941211882	3.7377205300550465
3	2.8412462881353435	1.9967621200835652
4	1.3266767733943416	1.2431436339612014
5	0.8154757083057858	0.955162417629259
6	0.5778419349092633	0.8121878711048562
7	0.396217895011608	0.649723117578743
8	0.3009413571198994	0.5639856516701764
9	0.25944053166106074	0.5469863998046374
>10	3.648745409907804	21.205255725033105
>50	0.9274458065479253	14.880887254990919
>100	0.47425822876149026	21.744006860814665
>500	0.02540153629389715	3.887864643840228
>1k	0.014721344897599482	5.7900755853991095
>5k	0.0023092305721724676	3.149118970507222
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAA	7459	0.4975914280053448	No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAA	6316	0.4213416623249441	No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATGCTTGGGA	5891	0.39298982469224913	No Hit
ATGCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	5723	0.38178251005156033	No Hit
ATGCTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	5707	0.3807151467524471	No Hit
ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	5237	0.3493613498409962	No Hit
ATGCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	5202	0.34702649262418606	No Hit
ATGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5036	0.33595259839588637	No Hit
ATGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	4798	0.3200755693215772	No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAA	4204	0.2804497068419989	No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAA	3774	0.25176431817833106	No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAA	3697	0.24662763230134868	No Hit
ATGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	3547	0.23662110137216225	No Hit
ATGCTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	3530	0.2354870278668544	No Hit
ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3161	0.21087096178105577	No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAA	2329	0.15536807022716825	No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAA	2271	0.15149887826788283	No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGAAAAAAAAAAAAAAAA	2247	0.14989783331921303	No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAA	2133	0.1422928698130313	No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAA	1914	0.1276833346564191	No Hit
ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTG	1798	0.11994495073784824	No Hit
ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAA	1663	0.11093907290158043	No Hit
ATGCTTGGGGGGGCTGGAGAGATGGCTCAGTGGTTATGAGCACCGACTGC	1615	0.10773698300424077	No Hit
ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTG	1512	0.10086583176619941	No Hit
ATGCTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	1511	0.10079912156000483	No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGCAAAAAAAAAAAAAAAA	1504	0.1003321501166428	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.002668408247783053	0.0	0.0	0.0	0.0
8	0.004336163402647461	0.0	0.0	0.0	0.0
9	0.007071281856625091	0.0	0.0	0.0	0.0
10	0.07951856578393499	0.0	0.0	0.0	0.0
11	0.22648115003058664	0.0	0.0	0.0	0.0
12	0.3091350955056667	0.0	0.0	0.0	0.0
13	0.37864713036041525	0.0	0.0	0.0	0.0
14	0.46336909222752715	0.0	0.0	0.0	0.0
15	0.5608327034778032	0.0	0.0	0.0	0.0
16	0.6667685109147904	0.0	0.0	0.0	0.0
17	0.7411503908217429	0.0	0.0	0.0	0.0
18	0.8147317482543607	0.0	0.0	0.0	0.0
19	0.8877794240374217	0.0	0.0	0.0	0.0
20	0.9717008634301988	0.0	0.0	0.0	0.0
21	1.056823086534478	0.0	0.0	0.0	0.0
22	1.1594233836617365	0.0	0.0	0.0	0.0
23	1.2749654607907428	0.0	0.0	0.0	0.0
24	1.3970451381268174	0.0	0.0	0.0	0.0
25	1.5186578440195302	0.0	0.0	0.0	0.0
26	1.6586825668219458	0.0	0.0	0.0	0.0
27	1.79737308550047	0.0	0.0	0.0	0.0
28	1.951540372016136	0.0	0.0	0.0	0.0
29	2.0629464163610782	0.0	0.0	0.0	0.0
30	2.164546060395418	0.0	0.0	0.0	0.0
31	2.251936430510313	0.0	0.0	0.0	0.0
32	2.3421286292853805	0.0	0.0	0.0	0.0
33	2.431587015792307	0.0	0.0	0.0	0.0
34	2.521979345185958	0.0	0.0	0.0	0.0
35	2.6374547121087697	0.0	0.0	0.0	0.0
36	2.7263127067599453	0.0	0.0	0.0	0.0
37	2.8191733137827955	0.0	0.0	0.0	0.0
38	2.900960026577346	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGTC	30	2.5282334E-6	44.000004	39
AGTACGG	30	2.5282334E-6	44.000004	12
TAATCGT	30	2.5282334E-6	44.000004	30
AACGTTG	25	4.443491E-5	44.0	18
AAGTACG	25	4.443491E-5	44.0	44
GTACCGG	35	1.4464786E-7	44.0	26
ACGTTTA	20	7.85725E-4	44.0	10
ACGTTAG	20	7.85725E-4	44.0	39
CGATTAC	20	7.85725E-4	44.0	44
TACGGAA	35	1.4464786E-7	44.0	31
TATGCGC	25	4.443491E-5	44.0	40
TACGCAC	45	4.802132E-10	44.0	16
TAATTCG	20	7.85725E-4	44.0	43
CGTAGCA	20	7.85725E-4	44.0	32
AACGATA	45	4.802132E-10	44.0	44
CATACGT	25	4.443491E-5	44.0	30
CGTAATT	35	1.4464786E-7	44.0	40
CATAACG	25	4.443491E-5	44.0	33
TATAGGG	20	7.85725E-4	44.0	13
CATCGCG	25	4.443491E-5	44.0	28
>>END_MODULE