FastQCFastQC Report
Fri 17 Jun 2016
SRR1527518_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527518_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1280997
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAA64440.5030456745800341No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAA55080.42997758776952644No Hit
ATGCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG49350.38524680385668353No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATGCTTGGGAAG49270.38462229029420053No Hit
ATGCTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC48650.3797823101849575No Hit
ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT45180.35269403441225855No Hit
ATGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC43300.33801796569390874No Hit
ATGCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT41680.32537156605362855No Hit
ATGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC40510.31623805520231507No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAA34710.2709608219222996No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAA34680.2707266293363685No Hit
ATGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA33640.26260795302408985No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAA31840.24855639786822295No Hit
ATGCTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC29960.23388032914987308No Hit
ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26720.20858752986931273No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAA20750.16198320526902094No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGAAAAAAAAAAAAAAAAAA20210.15776773872226085No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAA19660.15347420798019043No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAA18890.14746326494129183No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAA16480.12864979387149228No Hit
ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT15530.12123369531700698No Hit
ATGCTTGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA14510.11327114739534908Illumina Single End Adapter 2 (97% over 34bp)
ATGCTTGGGGGGGCTGGAGAGATGGCTCAGTGGTTATGAGCACCGACTGCTC14390.11233437705162462No Hit
ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAA13990.10921180923920977No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGCAAAAAAAAAAAAAAAAAA13470.10515247108307045No Hit
ATGCTTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC13410.10468408591120823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATA351.0196891E-746.00000432
ATTCGTC351.0196891E-746.00000439
AGTATCG351.0196891E-746.00000428
CCGTAAA351.0196891E-746.00000414
AACGATA700.046.00000444
CGTAATT351.0196891E-746.00000440
ACGTAAT351.0196891E-746.00000439
CACGTTA351.0196891E-746.00000438
AATCACG206.3117256E-446.017
TTTGTCG206.3117256E-446.042
GAACTAG301.861572E-646.031
TAGCGAA206.3117256E-446.032
TTCGTGC650.046.034
CGTCTAT206.3117256E-446.041
TGACGTA253.417377E-546.019
TCGATAG206.3117256E-446.019
TCGATAA301.861572E-646.046
TCGGCTA253.417377E-546.036
GTAGCGT405.6115823E-946.010
ACCTAAT253.417377E-546.010
Produced by FastQC (version 0.11.5)