##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527518_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1280997
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.430809752091534	31.0	31.0	33.0	30.0	34.0
2	32.08728435741848	33.0	31.0	34.0	30.0	34.0
3	32.63973998377826	34.0	31.0	34.0	31.0	34.0
4	36.28010604240291	37.0	37.0	37.0	35.0	37.0
5	36.354485607694635	37.0	37.0	37.0	35.0	37.0
6	36.531523493029255	37.0	37.0	37.0	35.0	37.0
7	36.56295604127098	37.0	37.0	37.0	35.0	37.0
8	36.62697804912892	37.0	37.0	37.0	35.0	37.0
9	38.6370709689406	39.0	39.0	39.0	39.0	39.0
10	37.63573685184274	39.0	37.0	39.0	35.0	39.0
11	37.55599739890101	39.0	37.0	39.0	35.0	39.0
12	37.28158145569427	39.0	37.0	39.0	35.0	39.0
13	37.10399946291834	39.0	37.0	39.0	34.0	39.0
14	37.941739910397914	40.0	38.0	40.0	34.0	40.0
15	38.10797293045963	40.0	38.0	40.0	35.0	40.0
16	38.248659442606034	40.0	38.0	40.0	35.0	40.0
17	38.29510685817375	40.0	38.0	40.0	35.0	40.0
18	38.17059758922152	40.0	38.0	40.0	35.0	40.0
19	38.09556618789896	40.0	38.0	40.0	34.0	40.0
20	38.121846499250196	40.0	38.0	40.0	34.0	40.0
21	38.09139677922743	40.0	38.0	40.0	34.0	40.0
22	38.03754185216671	40.0	38.0	40.0	34.0	40.0
23	37.96725675391902	40.0	38.0	40.0	34.0	40.0
24	37.82416274198925	39.0	37.0	40.0	34.0	40.0
25	37.5857624959309	39.0	37.0	40.0	34.0	40.0
26	37.29399834660035	39.0	35.0	40.0	34.0	40.0
27	37.163325128786404	39.0	35.0	40.0	33.0	40.0
28	37.02408046232739	39.0	35.0	40.0	33.0	40.0
29	36.92354158518716	39.0	35.0	40.0	33.0	40.0
30	36.6396627002249	39.0	35.0	40.0	33.0	40.0
31	36.478430472514766	39.0	35.0	40.0	32.0	40.0
32	36.052105508443816	38.0	35.0	40.0	31.0	40.0
33	35.60977035855665	38.0	35.0	40.0	30.0	40.0
34	35.03219679671381	38.0	35.0	40.0	25.0	40.0
35	34.69891732767524	38.0	34.0	40.0	23.0	40.0
36	34.33783763740274	38.0	34.0	40.0	21.0	40.0
37	33.852049614479974	37.0	34.0	40.0	18.0	40.0
38	33.60601703204613	38.0	33.0	40.0	15.0	40.0
39	33.07814304014764	37.0	33.0	40.0	10.0	40.0
40	32.54460549087937	37.0	33.0	40.0	10.0	40.0
41	32.15739459186868	37.0	32.0	40.0	9.0	40.0
42	31.788089277336326	36.0	31.0	40.0	9.0	40.0
43	31.582976384800276	35.0	31.0	40.0	9.0	40.0
44	31.340811102602114	35.0	31.0	40.0	8.0	40.0
45	31.17857887255005	35.0	30.0	40.0	8.0	40.0
46	31.023907940455754	35.0	30.0	40.0	8.0	40.0
47	30.85783885520419	35.0	30.0	39.0	8.0	40.0
48	30.637369174166682	35.0	29.0	39.0	8.0	40.0
49	30.411872939593145	35.0	28.0	39.0	8.0	40.0
50	30.529085548209714	35.0	29.0	39.0	8.0	40.0
51	30.502496883287	35.0	29.0	39.0	8.0	40.0
52	30.14544296356666	35.0	27.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	15.0
12	25.0
13	49.0
14	56.0
15	87.0
16	199.0
17	367.0
18	664.0
19	1204.0
20	1878.0
21	3412.0
22	5482.0
23	9078.0
24	13784.0
25	20209.0
26	30366.0
27	43415.0
28	52906.0
29	48573.0
30	32706.0
31	25392.0
32	28757.0
33	37956.0
34	72138.0
35	78965.0
36	133414.0
37	204194.0
38	342104.0
39	93599.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.46799250895981	0.26198343946160685	0.20140562390075856	0.06861842767781658
8	99.12357327925045	0.511008222501692	0.21584750003317726	0.14957099821467185
9	97.32021230338557	1.5152260309743113	0.5746305416796448	0.5899311239604776
10	60.73410007985968	28.599988914884268	3.972218514172945	6.693692491083117
11	36.127094755100906	34.60921454148605	16.089030653467574	13.174660049945471
12	34.7466075252323	27.100375722972032	21.579753894817866	16.57326285697781
13	23.713170288455007	28.87204263554091	25.22066796409359	22.19411911191049
14	24.621915586063043	32.164009751779275	21.519176079257015	21.694898582900663
15	22.153681858739716	33.21483188485219	21.584281618145866	23.04720463826223
16	26.564543086361635	35.69391653532365	20.163825520278344	17.57771485803636
17	30.266425292174766	32.99828180706122	17.721665234188684	19.01362766657533
18	25.478357872813127	33.505933269164565	22.09177695185859	18.923931906163716
19	26.95158536671046	33.30359087492008	19.450709096118104	20.294114662251356
20	21.68599926463528	35.99579077858886	19.969133417174277	22.349076539601576
21	23.691780698939965	35.46854520346262	23.732217952110737	17.10745614548668
22	22.82909327656505	36.49766549023924	20.70855747515412	19.964683758041588
23	23.89849468812183	34.746685589427614	23.63354480923843	17.72127491321213
24	26.880546948978022	34.12107912821029	19.00277674342719	19.995597179384493
25	22.89248140315707	31.613344918059916	20.771477216574276	24.72269646220873
26	23.88210120710665	30.971657232608663	23.8628974150603	21.283344145224383
27	23.657822773979955	30.561586014643282	23.063988440253958	22.716602771122805
28	29.856900523576556	33.00874240923281	18.047427121218863	19.08692994597177
29	31.97087893258142	32.26721061797959	17.30909596197337	18.45281448746562
30	31.68524204194077	32.49547032506712	16.93157751345241	18.8877101195397
31	32.160887183966864	32.357608956149	17.206831866116783	18.274671993767356
32	31.5251323773592	33.60538705398998	18.143211888864688	16.726268679786134
33	30.809674027339646	35.47361937615779	16.328609668875103	17.388096927627466
34	25.391160166651446	37.801805937094315	18.73813912132503	18.068894774929216
35	24.56680226417392	39.917814015177235	19.41425311690816	16.10113060374068
36	25.824962899991178	39.60102951060775	16.41650995279458	18.157497636606486
37	26.269616556479054	39.21952978812597	16.99199920062264	17.518854454772338
38	28.87563358852519	37.00828339176439	16.40331710378713	17.7127659159233
39	27.58999435595868	35.37916169983224	16.770062693355253	20.260781250853828
40	27.987341110088472	33.821624874999706	18.016982085047818	20.174051929864003
41	27.293350413779265	32.937469798914435	18.866945043587144	20.90223474371915
42	27.31606709461459	31.2940623592405	19.68349652653363	21.70637401961129
43	28.11591283976465	29.854948918693797	20.312303619758673	21.716834621782876
44	27.313412911974034	29.271887443920637	19.837517183880994	23.577182460224343
45	28.2810966770414	29.402098521698335	20.152506211958343	22.164298589301925
46	27.086324167816162	31.22731747225013	20.637831314202923	21.048527045730786
47	27.631758700449726	29.790858214343984	19.878110565442388	22.699272519763902
48	27.792414814398477	29.22473666995317	20.3320538611722	22.65079465447616
49	27.801938646226336	28.719505197904443	20.920501765421776	22.55805439044744
50	24.7826497642071	29.473136939430773	21.63135432791802	24.112858968444108
51	24.519339233425214	29.850108938584558	21.6311981995274	23.99935362846283
52	25.078278871847477	28.96220678112439	21.73478938670426	24.224724960323872
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	39.5
4	79.0
5	59.0
6	39.0
7	59.0
8	79.0
9	828.0
10	1577.0
11	2845.5
12	4114.0
13	3732.5
14	3099.5
15	2848.0
16	3331.0
17	3814.0
18	4247.5
19	4681.0
20	5456.5
21	6232.0
22	8054.0
23	9876.0
24	11946.0
25	14016.0
26	18100.0
27	22184.0
28	26736.0
29	31288.0
30	35860.5
31	40433.0
32	46469.5
33	52506.0
34	57526.0
35	62546.0
36	60489.5
37	58433.0
38	55181.0
39	48546.0
40	45163.0
41	44067.0
42	42971.0
43	41711.0
44	40451.0
45	43739.5
46	47028.0
47	44862.5
48	42697.0
49	44207.0
50	45717.0
51	51189.5
52	56662.0
53	59795.0
54	62928.0
55	72147.5
56	81367.0
57	85895.5
58	90424.0
59	81441.0
60	72458.0
61	73151.5
62	73845.0
63	66679.0
64	52550.5
65	45588.0
66	37881.5
67	30175.0
68	26935.0
69	23695.0
70	20809.0
71	17923.0
72	18426.5
73	18930.0
74	14000.0
75	9070.0
76	5879.5
77	2689.0
78	1760.0
79	831.0
80	777.5
81	724.0
82	402.0
83	80.0
84	57.0
85	34.0
86	20.0
87	6.0
88	4.5
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1280997.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.496397162728215
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.69925089272208	17.70449604491869
2	8.235102395904303	3.705202683479958
3	2.9429191500431915	1.9861523405151054
4	1.463781210439519	1.3171921387754586
5	0.9093428439949724	1.0228468887797852
6	0.6185723778658386	0.8349389931817858
7	0.47964847887500656	0.755325387548942
8	0.39540150517565775	0.7116087439337706
9	0.3196565176413387	0.6472007978862773
>10	4.513432961930113	24.692954835836776
>50	0.8968805329505116	13.705342144766306
>100	0.48503689644055836	20.95220830869369
>500	0.025915328762888934	4.0828227309459875
>1k	0.014358492963222247	6.940081708296162
>5k	7.004142908888901E-4	0.9416262524413185
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAA	6444	0.5030456745800341	No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAA	5508	0.42997758776952644	No Hit
ATGCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	4935	0.38524680385668353	No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATGCTTGGGAAG	4927	0.38462229029420053	No Hit
ATGCTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	4865	0.3797823101849575	No Hit
ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	4518	0.35269403441225855	No Hit
ATGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4330	0.33801796569390874	No Hit
ATGCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	4168	0.32537156605362855	No Hit
ATGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	4051	0.31623805520231507	No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAA	3471	0.2709608219222996	No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAA	3468	0.2707266293363685	No Hit
ATGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	3364	0.26260795302408985	No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAA	3184	0.24855639786822295	No Hit
ATGCTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	2996	0.23388032914987308	No Hit
ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2672	0.20858752986931273	No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAA	2075	0.16198320526902094	No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGAAAAAAAAAAAAAAAAAA	2021	0.15776773872226085	No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAA	1966	0.15347420798019043	No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAA	1889	0.14746326494129183	No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAA	1648	0.12864979387149228	No Hit
ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1553	0.12123369531700698	No Hit
ATGCTTGGATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA	1451	0.11327114739534908	Illumina Single End Adapter 2 (97% over 34bp)
ATGCTTGGGGGGGCTGGAGAGATGGCTCAGTGGTTATGAGCACCGACTGCTC	1439	0.11233437705162462	No Hit
ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAA	1399	0.10921180923920977	No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGCAAAAAAAAAAAAAAAAAA	1347	0.10515247108307045	No Hit
ATGCTTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	1341	0.10468408591120823	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.001639348101517802	0.0	0.0	0.0	0.0
8	0.005074172695174149	0.0	0.0	0.0	0.0
9	0.009367703437244584	0.0	0.0	0.0	0.0
10	0.08797834811478872	0.0	0.0	0.0	0.0
11	0.2440286745402214	0.0	0.0	0.0	0.0
12	0.33825215827983984	0.0	0.0	0.0	0.0
13	0.4155357116371077	0.0	0.0	0.0	0.0
14	0.5093688744001742	0.0	0.0	0.0	0.0
15	0.6184245552487633	0.0	0.0	0.0	0.0
16	0.7338034359174924	0.0	0.0	0.0	0.0
17	0.8184250236339351	0.0	0.0	0.0	0.0
18	0.8968014757255481	0.0	0.0	0.0	0.0
19	0.979549522754542	0.0	0.0	0.0	0.0
20	1.066513036330296	0.0	0.0	0.0	0.0
21	1.159019107773086	0.0	0.0	0.0	0.0
22	1.26065869006719	0.0	0.0	0.0	0.0
23	1.3625324649472248	0.0	0.0	0.0	0.0
24	1.4623765707491898	0.0	0.0	0.0	0.0
25	1.5544923212154282	0.0	0.0	0.0	0.0
26	1.6362255337053873	0.0	0.0	0.0	0.0
27	1.7121039315470685	0.0	0.0	0.0	0.0
28	1.8203789704425537	0.0	0.0	0.0	0.0
29	1.880332272440919	0.0	0.0	0.0	0.0
30	1.9378655843846628	0.0	0.0	0.0	0.0
31	1.9774441314070212	0.0	0.0	0.0	0.0
32	2.0231116856635887	0.0	0.0	0.0	0.0
33	2.067920533771742	0.0	0.0	0.0	0.0
34	2.121706764340588	0.0	0.0	0.0	0.0
35	2.2121831667053082	0.0	0.0	0.0	0.0
36	2.2733854958286397	0.0	0.0	0.0	0.0
37	2.3326362200692117	0.0	0.0	0.0	0.0
38	2.387749541958334	0.0	0.0	0.0	0.0
39	2.446375752636423	0.0	0.0	0.0	0.0
40	2.5042993855567186	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGATA	35	1.0196891E-7	46.000004	32
ATTCGTC	35	1.0196891E-7	46.000004	39
AGTATCG	35	1.0196891E-7	46.000004	28
CCGTAAA	35	1.0196891E-7	46.000004	14
AACGATA	70	0.0	46.000004	44
CGTAATT	35	1.0196891E-7	46.000004	40
ACGTAAT	35	1.0196891E-7	46.000004	39
CACGTTA	35	1.0196891E-7	46.000004	38
AATCACG	20	6.3117256E-4	46.0	17
TTTGTCG	20	6.3117256E-4	46.0	42
GAACTAG	30	1.861572E-6	46.0	31
TAGCGAA	20	6.3117256E-4	46.0	32
TTCGTGC	65	0.0	46.0	34
CGTCTAT	20	6.3117256E-4	46.0	41
TGACGTA	25	3.417377E-5	46.0	19
TCGATAG	20	6.3117256E-4	46.0	19
TCGATAA	30	1.861572E-6	46.0	46
TCGGCTA	25	3.417377E-5	46.0	36
GTAGCGT	40	5.6115823E-9	46.0	10
ACCTAAT	25	3.417377E-5	46.0	10
>>END_MODULE