FastQCFastQC Report
Fri 17 Jun 2016
SRR1527517_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527517_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1599814
Sequences flagged as poor quality0
Sequence length52
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAA77420.48393125700862727No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATGCTTGGGAAG70050.43786340162043835No Hit
ATGCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG65230.40773489918202993No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAA64800.4050470867238316No Hit
ATGCTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC63700.3981712874121616No Hit
ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT57450.35910424586858225No Hit
ATGCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT56170.35110331576045717No Hit
ATGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC51060.3191621025944266No Hit
ATGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC50310.31447405760919706No Hit
ATGCTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC41910.2619679537746263No Hit
ATGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA41000.2562797925258811No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAA40900.2556547198611839No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAA38540.2409030049743283No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAA37680.23552738005793175No Hit
ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33160.20727409561361507No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAA23980.14989242499440558No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGAAAAAAAAAAAAAAAAAA23830.14895481599735969No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAA23120.14451680007800907No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAA21590.13495318830814082No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAA20660.1291400125264562No Hit
ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT19400.12126409695127059No Hit
ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAA17040.10651238206441499No Hit
ATGCTTGGGGGGGCTGGAGAGATGGCTCAGTGGTTATGAGCACCGACTGCTC16890.10557477306736907No Hit
ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCT16660.10413710593856537No Hit
ATGCTTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC16490.10307448240858No Hit
ATGCTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC16420.1026369315432919No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGCAAAAAAAAAAAAAAAAAA16130.10082422081566982No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTACG351.01990736E-746.00000420
ACGTTTA206.312199E-446.00000410
CTATAAT206.312199E-446.00000444
ATGTCGC405.6134013E-946.00000438
TCTAGCG206.312199E-446.00000427
CGTTAGT206.312199E-446.00000428
TAATTCG206.312199E-446.00000443
CATCCGA405.6134013E-946.00000431
TTCGAAT206.312199E-446.00000411
TACGTAC206.312199E-446.00000423
TAATCGC405.6134013E-946.00000435
ATGTACG551.8189894E-1246.038
TACGCAC650.046.016
CGTAATA253.4177603E-546.024
TCTATCG253.4177603E-546.042
GCGAAGT301.8618648E-645.99999635
CCGTAAA301.8618648E-645.99999614
TCGGTAT301.8618648E-645.99999636
TGGGATA18000.043.4444476
TTGGGAT101100.043.383785
Produced by FastQC (version 0.11.5)