##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527517_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1599814
Sequences flagged as poor quality	0
Sequence length	52
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.54946950082947	31.0	30.0	31.0	25.0	33.0
2	30.607695644618687	31.0	31.0	33.0	28.0	34.0
3	31.443868474710186	31.0	31.0	34.0	28.0	34.0
4	35.7310943647199	37.0	35.0	37.0	33.0	37.0
5	35.88748879557249	37.0	35.0	37.0	35.0	37.0
6	36.22286965859781	37.0	36.0	37.0	35.0	37.0
7	36.348213604831564	37.0	36.0	37.0	35.0	37.0
8	36.460978588760945	37.0	37.0	37.0	35.0	37.0
9	38.52767384208414	39.0	39.0	39.0	37.0	39.0
10	37.09884524075924	39.0	37.0	39.0	33.0	39.0
11	36.895295328081886	39.0	37.0	39.0	33.0	39.0
12	36.63591517513911	39.0	35.0	39.0	33.0	39.0
13	36.42559447535776	39.0	35.0	39.0	32.0	39.0
14	37.28075138734878	39.0	36.0	40.0	33.0	40.0
15	37.45125120795292	40.0	36.0	40.0	33.0	40.0
16	37.56146464526501	40.0	37.0	40.0	33.0	40.0
17	37.66494604997831	40.0	37.0	40.0	33.0	40.0
18	37.56661524402212	40.0	36.0	40.0	33.0	40.0
19	37.44091875680548	40.0	36.0	40.0	33.0	40.0
20	37.441768230556804	40.0	36.0	40.0	33.0	40.0
21	37.49198719351124	40.0	36.0	40.0	33.0	40.0
22	37.445795573735445	40.0	36.0	40.0	33.0	40.0
23	37.396056041514825	39.0	36.0	40.0	33.0	40.0
24	37.300599944743574	39.0	36.0	40.0	33.0	40.0
25	37.07566442098894	39.0	36.0	40.0	33.0	40.0
26	36.76186856722094	38.0	35.0	40.0	33.0	40.0
27	36.610505971319164	38.0	35.0	40.0	32.0	40.0
28	36.3780264455743	38.0	35.0	40.0	31.0	40.0
29	36.34093900915982	38.0	35.0	40.0	31.0	40.0
30	36.09095119807677	38.0	35.0	40.0	31.0	40.0
31	35.89994774392523	38.0	35.0	40.0	30.0	40.0
32	35.48486324035169	38.0	35.0	40.0	30.0	40.0
33	35.10757625573973	37.0	34.0	40.0	27.0	40.0
34	34.61484710097549	37.0	34.0	40.0	24.0	40.0
35	34.308136445861834	37.0	33.0	40.0	23.0	40.0
36	34.008644129880096	37.0	33.0	40.0	21.0	40.0
37	33.60704431890207	37.0	33.0	40.0	18.0	40.0
38	33.24109740257305	37.0	33.0	40.0	15.0	40.0
39	32.70122901787333	36.0	32.0	40.0	12.0	40.0
40	32.40231301888844	36.0	32.0	40.0	10.0	40.0
41	32.18219743045129	36.0	32.0	40.0	10.0	40.0
42	31.958763331237257	36.0	31.0	40.0	9.0	40.0
43	31.74762628655581	35.0	31.0	40.0	9.0	40.0
44	31.527248167599485	35.0	31.0	40.0	9.0	40.0
45	31.391353619858307	35.0	31.0	40.0	9.0	40.0
46	31.150113700717707	35.0	30.0	40.0	8.0	40.0
47	31.04409887649439	35.0	30.0	39.0	8.0	40.0
48	30.885627329177016	35.0	30.0	39.0	8.0	40.0
49	30.676177980690255	35.0	29.0	39.0	8.0	40.0
50	30.466676751172322	35.0	28.0	39.0	8.0	40.0
51	30.292204593784028	35.0	27.0	39.0	8.0	40.0
52	29.804917946711306	35.0	26.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	5.0
11	7.0
12	17.0
13	38.0
14	71.0
15	137.0
16	322.0
17	668.0
18	1301.0
19	2338.0
20	3739.0
21	5865.0
22	8979.0
23	13323.0
24	19348.0
25	27086.0
26	38687.0
27	53717.0
28	64958.0
29	61061.0
30	45346.0
31	41077.0
32	48613.0
33	65507.0
34	107771.0
35	115640.0
36	181693.0
37	264761.0
38	370024.0
39	57715.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	100.0	0.0
7	99.4780018177113	0.2540920381994407	0.20808669007772151	0.059819454011528835
8	99.13577453378956	0.5054962639406831	0.22108820150342476	0.1376410007663391
9	97.37381970654089	1.5084253544474546	0.5455009144813084	0.5722540245303516
10	60.239440334938934	29.015935602513792	3.916642809726631	6.82798125282064
11	35.50975300878727	34.4694445729316	16.418096103672053	13.602706314609073
12	34.54382822003058	26.684789606791792	21.800221775781434	16.971160397396197
13	23.391906809166567	28.35204592533882	25.48983819369002	22.766209071804596
14	24.46671925611352	31.617238003917958	21.856853359203008	22.059189380765513
15	22.461423640498207	32.2239960395396	22.03487405410879	23.279706265853406
16	26.74192124834512	34.73610057169146	20.646587665816153	17.87539051414727
17	30.51385973619433	32.198805611152295	17.84051145945716	19.44682319319621
18	25.842191654779867	32.730054868878504	22.125697112289302	19.302056364052323
19	27.036830531549295	32.443771588447156	19.8864367982778	20.63296108172575
20	21.860166244325903	35.41424190562153	20.116526046152867	22.609065803899703
21	24.003790440638724	34.506823918280496	24.067297823371966	17.42208781770881
22	23.002486539060165	35.531943088384025	20.804105977319864	20.661464395235946
23	24.09430096248689	34.14409425095668	23.600556064642515	18.16104872191392
24	26.818117606171715	33.68847878565883	19.26298932250874	20.230414285660707
25	23.141315177889428	30.87271395299704	20.90080471854853	25.085166150565
26	24.362769671974366	30.246703679302716	23.681440467454344	21.70908618126857
27	23.723695379587877	29.88847453516471	22.992735405490887	23.39509467975652
28	29.799089144113005	32.61047846812192	18.170237290084973	19.420195097680104
29	31.958777707908542	31.665868657231407	17.584356681464218	18.790996953395833
30	31.54222928415428	32.17517786442674	17.027166908153074	19.255425943265905
31	32.11560843948109	31.816698691222854	17.445715564434366	18.62197730486169
32	31.41483947508898	32.89369889249625	18.595724252944404	17.095737379470364
33	30.892028698336183	34.78135583261554	16.596866885775473	17.729748583272805
34	25.345196379079066	37.17900955986133	18.95426593341476	18.52152812764484
35	24.42421431491411	39.534220853174176	19.622218582910264	16.419346249001446
36	25.741617463030074	39.10242065640131	16.64731024981654	18.508651630752073
37	25.87638312953881	38.85620453377705	17.44277772291029	17.824634613773853
38	28.937363968561346	36.423421722775274	16.558987482294818	18.080226826368566
39	27.01951601873718	35.12870871238781	17.044481420965187	20.80729384790982
40	27.198411815373536	33.56827731223755	18.558782458460797	20.67452841392812
41	26.488954340942133	32.80700131390274	19.24054921384611	21.463495131309017
42	26.282492839792624	31.24081924523726	20.089335385238535	22.38735252973158
43	27.26879499741845	29.799401680445353	20.59851957790093	22.333283744235267
44	26.53408458733328	29.225584974253255	20.059456911866004	24.18087352654746
45	27.28723464102702	29.55231045609052	20.632461023593994	22.527993879288466
46	26.608718263498133	31.16712317806945	21.04494647502772	21.179212083404696
47	27.160594919159355	29.9099770348303	20.13184032643795	22.7975877195724
48	27.530762951193076	29.386416170879865	20.435125583349066	22.647695294577993
49	27.430251266709753	28.96561725300566	21.167460717308387	22.436670762976195
50	24.79000683829495	29.423545487162883	21.523377092587012	24.26307058195515
51	24.483971261659168	29.94191824799633	21.32672923227325	24.24738125807125
52	25.25399827729974	28.93830157755839	21.4965614752715	24.31113866987037
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	58.0
4	116.0
5	82.5
6	49.0
7	75.0
8	101.0
9	983.5
10	1866.0
11	3458.5
12	5051.0
13	4591.5
14	3977.5
15	3823.0
16	4464.5
17	5106.0
18	5912.0
19	6718.0
20	8057.5
21	9397.0
22	11978.5
23	14560.0
24	17741.5
25	20923.0
26	26002.5
27	31082.0
28	36518.0
29	41954.0
30	45995.5
31	50037.0
32	55449.5
33	60862.0
34	66132.5
35	71403.0
36	68802.5
37	66202.0
38	62137.0
39	55078.5
40	52085.0
41	51624.0
42	51163.0
43	49851.5
44	48540.0
45	52898.0
46	57256.0
47	55588.5
48	53921.0
49	56170.0
50	58419.0
51	65753.0
52	73087.0
53	77088.0
54	81089.0
55	92100.0
56	103111.0
57	109811.0
58	116511.0
59	104636.5
60	92762.0
61	93474.5
62	94187.0
63	84769.5
64	66821.0
65	58290.0
66	48143.5
67	37997.0
68	33911.5
69	29826.0
70	26555.5
71	23285.0
72	23973.0
73	24661.0
74	18019.0
75	11377.0
76	7370.5
77	3364.0
78	2219.0
79	1074.0
80	978.0
81	882.0
82	479.0
83	76.0
84	52.5
85	29.0
86	19.5
87	10.0
88	7.5
89	3.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1599814.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.610487104296542
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.79102376210409	19.311259117823706
2	7.532590158849403	3.556962456149298
3	2.6375623184306143	1.8682239331825354
4	1.2378691713134458	1.1690677642440943
5	0.7351739491789017	0.867890752325161
6	0.4799672618728815	0.6799356508160515
7	0.355511365896829	0.5875657563956552
8	0.3006165368173914	0.5678162292692245
9	0.23593926845535995	0.5013576949736193
>10	3.188607238930548	18.92442551077031
>50	0.9685760933596181	15.777599461071098
>100	0.4938395202710031	22.93025522619328
>500	0.026005520142522148	4.081115028722404
>1k	0.014329572323430574	5.691807791140586
>5k	0.002388262053905095	3.484717626923015
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAA	7742	0.48393125700862727	No Hit
ATGCTTGGGAAGCAGTGGTATCAACGCAGAGTGCAGTGCTATGCTTGGGAAG	7005	0.43786340162043835	No Hit
ATGCTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	6523	0.40773489918202993	No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAA	6480	0.4050470867238316	No Hit
ATGCTTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	6370	0.3981712874121616	No Hit
ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	5745	0.35910424586858225	No Hit
ATGCTTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	5617	0.35110331576045717	No Hit
ATGCTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	5106	0.3191621025944266	No Hit
ATGCTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5031	0.31447405760919706	No Hit
ATGCTTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	4191	0.2619679537746263	No Hit
ATGCTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	4100	0.2562797925258811	No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAA	4090	0.2556547198611839	No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAA	3854	0.2409030049743283	No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGAAAAAAAAAAAAAAAAAAAAA	3768	0.23552738005793175	No Hit
ATGCTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3316	0.20727409561361507	No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGTAAAAAAAAAAAAAAAAAAA	2398	0.14989242499440558	No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGGAAAAAAAAAAAAAAAAAA	2383	0.14895481599735969	No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGAAAAAAAAAAAAAAAAAAAAAA	2312	0.14451680007800907	No Hit
ATGCTTGGGAGTGCAGTGCTATGCTTGGGGGGAAAAAAAAAAAAAAAAAAAA	2159	0.13495318830814082	No Hit
ATGCTTGGGCAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAA	2066	0.1291400125264562	No Hit
ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCTGACTGCT	1940	0.12126409695127059	No Hit
ATGCTTGGGAGAGTGCAGTGCTATGCTTGGGGAAAAAAAAAAAAAAAAAAAA	1704	0.10651238206441499	No Hit
ATGCTTGGGGGGGCTGGAGAGATGGCTCAGTGGTTATGAGCACCGACTGCTC	1689	0.10557477306736907	No Hit
ATGCTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCT	1666	0.10413710593856537	No Hit
ATGCTTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	1649	0.10307448240858	No Hit
ATGCTTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	1642	0.1026369315432919	No Hit
ATGCTTGGGGAGTGCAGTGCTATGCTTGGGGGGCAAAAAAAAAAAAAAAAAA	1613	0.10082422081566982	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0013126525958642693	0.0	0.0	0.0	0.0
8	0.0039379577875928076	0.0	0.0	0.0	0.0
9	0.005625653982275439	0.0	0.0	0.0	0.0
10	0.07375857443427798	0.0	0.0	0.0	0.0
11	0.21246219873060243	0.0	0.0	0.0	0.0
12	0.2974720811294313	0.0	0.0	0.0	0.0
13	0.36679263964435865	0.0	0.0	0.0	0.0
14	0.44430165006682026	0.0	0.0	0.0	0.0
15	0.5425005656907615	0.0	0.0	0.0	0.0
16	0.6421996557099763	0.0	0.0	0.0	0.0
17	0.7127078522878285	0.0	0.0	0.0	0.0
18	0.7810908018057099	0.0	0.0	0.0	0.0
19	0.8531616800453052	0.0	0.0	0.0	0.0
20	0.9368589098482699	0.0	0.0	0.0	0.0
21	1.0209936905165224	0.0	0.0	0.0	0.0
22	1.119130098873994	0.0	0.0	0.0	0.0
23	1.2207669141537705	0.0	0.0	0.0	0.0
24	1.3249040200923357	0.0	0.0	0.0	0.0
25	1.424478095578611	0.0	0.0	0.0	0.0
26	1.5246147364631137	0.0	0.0	0.0	0.0
27	1.6184381434341741	0.0	0.0	0.0	0.0
28	1.7314512812114409	0.0	0.0	0.0	0.0
29	1.8046472902474913	0.0	0.0	0.0	0.0
30	1.8776557774841325	0.0	0.0	0.0	0.0
31	1.9277866051928536	0.0	0.0	0.0	0.0
32	1.9796051290962575	0.0	0.0	0.0	0.0
33	2.0332363637272834	0.0	0.0	0.0	0.0
34	2.0904305125470835	0.0	0.0	0.0	0.0
35	2.175065351347094	0.0	0.0	0.0	0.0
36	2.2381351832150487	0.0	0.0	0.0	0.0
37	2.297642100894229	0.0	0.0	0.0	0.0
38	2.3497731611299812	0.0	0.0	0.0	0.0
39	2.406217222752145	0.0	0.0	0.0	0.0
40	2.4655991258983856	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	35	1.01990736E-7	46.000004	20
ACGTTTA	20	6.312199E-4	46.000004	10
CTATAAT	20	6.312199E-4	46.000004	44
ATGTCGC	40	5.6134013E-9	46.000004	38
TCTAGCG	20	6.312199E-4	46.000004	27
CGTTAGT	20	6.312199E-4	46.000004	28
TAATTCG	20	6.312199E-4	46.000004	43
CATCCGA	40	5.6134013E-9	46.000004	31
TTCGAAT	20	6.312199E-4	46.000004	11
TACGTAC	20	6.312199E-4	46.000004	23
TAATCGC	40	5.6134013E-9	46.000004	35
ATGTACG	55	1.8189894E-12	46.0	38
TACGCAC	65	0.0	46.0	16
CGTAATA	25	3.4177603E-5	46.0	24
TCTATCG	25	3.4177603E-5	46.0	42
GCGAAGT	30	1.8618648E-6	45.999996	35
CCGTAAA	30	1.8618648E-6	45.999996	14
TCGGTAT	30	1.8618648E-6	45.999996	36
TGGGATA	1800	0.0	43.444447	6
TTGGGAT	10110	0.0	43.38378	5
>>END_MODULE