FastQCFastQC Report
Fri 17 Jun 2016
SRR1527516_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527516_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14107
Sequences flagged as poor quality0
Sequence length50
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC5363.799532147160984No Hit
GTCGGTGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT4293.041043453604593No Hit
GTCGGTGGGAAAACTGGCCTGTAAAAGCAGCTGTTCTGAGCCCAGTGCCC4012.842560430991706No Hit
GTCGGTGGGGTGTGAACGTCAGCTCGGCCATGGGCTGCAGCCAATCAGGG3132.2187566456369177No Hit
GTCGGTGGGAGGAAGCAGTGGTTCTTCTGAACAGGGCAACAACACTGAGA2992.119515134330474No Hit
GTCGGTGGGAGTTGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCAG2811.9919189055079038No Hit
GTCGGTGGGGGGGCTGGCGAGATGGCTTAGTGGGTAAGAGCACTGACTGC2601.8430566385482385No Hit
GTCGGTGGGGCTGGTGAGATGGCTCAGTGGTTAAAAGCGCCTACTGCTCT2261.6020415396611611No Hit
GTCGGTGGGAGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCGG2171.538243425249876No Hit
GTCGGTGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCAACTGC2121.5028000283547176No Hit
GTCGGTGGGGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCA2041.446090593322464No Hit
GTCGGTGAGCTACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACC1951.3822924789111788No Hit
GTCGGTGGGCAGAAAGAAATAGCAATGTATACATCACCTTCTTTATATCT1861.3184943644998937No Hit
GTCGGTGGGACTGACTGTTCTTCCAGAGGTCTTGAGTTCAAATCCCAGCA1681.1908981356773234No Hit
GTCGGTGGGGAGCCAATAGTGGAAGACATGCATCGCCAGTGGGCAAACCT1380.9782377543063727No Hit
GTCGGTGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTTCCCCAG1340.949883036790246No Hit
GTCGGTGGGTGGGTAAGGGGGACTTTTGGTATAGCATTGGAAATGTAAAT1290.9144396398950875No Hit
GTCGGTGGGAACCACCTCATGCCCACAAGGTACTCTGTGGATATCCCCTT1270.9002622811370242No Hit
GTCGGTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGAC1160.8222868079676756No Hit
GTCGGTGAGGGGGCTGGCGAGATGGCTTAGTGGGTAAGAGCACTGACTGC1110.7868434110725173No Hit
GTCGGTGGGAATCTATTTAAGGCGCTGCGCGGCCGGGCGTCTGATTCCCT1100.7797547316934855No Hit
GTCGGTGGGACATGCCCACCTGCAACTCCCTCCCTACCTCGCATAGCCAG1080.7655773729354222No Hit
GTCGGTGGAGAATGAGGCCAGATTTCCTGGGCACAGACTTTGTAAGCCTT1000.7088679379031687No Hit
GTCGGTGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGT960.6805132203870419No Hit
GTCGGTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGGTGGGCTGCAATA920.6521585028709151No Hit
GTCGGTGGGGTGGGGAGGAGCCTGGACAGGACCAGGACCCTGTGCAGTTG900.6379811441128518No Hit
GTCGGTGGGTGGGTATGGGGGACTTTTGGTATAGCATTGGAAATGTAAAT880.6238037853547884No Hit
GTCGGTGGGCATTTGTTTCGCCGCGAAACCGACACGCCTCCACGAAGCAA750.5316509534273764No Hit
GTCGGTGGGAGAGGACGACTATAAACAGGAAACCCAAAGCCTATCAGAAA730.5174735946693131No Hit
GTCGGTGGGGATTTTGCTAATCCAGAGGCTGAGTGGAGCCGAGCTGGTCA720.5103849152902814No Hit
GTCGGTGGGACTCGGGAGGGGGGGGCGCCAAGATCCTCTATCCCTGTGGG710.5032962359112497No Hit
GTCGGTGGGATATGTAGAGAATTTTCTAGTTCTCCCAATTATAACTTTGT700.49620755653221804No Hit
GTCGGTGGGTGTTCAAACACTGATAGAAATTCTAACATGAATAAAGATAA680.4820301977741547No Hit
GTCGGTGGGCAAATCTACCCATTCAGTGCGGGGCTGAAAATAGCAAGCTG680.4820301977741547No Hit
GTCGGTGGGGAAATTATCCATAGTTTTACAGACAGCTCCAGGCTATGAGC660.46785283901609126No Hit
GTCGGTGGGACTTCTAATTCTCTCCGAGCCCTCTTGGTCACCTCTGTGCT660.46785283901609126No Hit
GTCGGTGGGGGTGGGCCTGGTCTTTTGTGTGTGAGGCCCTTGACCGTGTG660.46785283901609126No Hit
GTCGGTGGGTATTGAGGATGGAATTTTTGAGGTCAAATCAACAGCTGGAG660.46785283901609126No Hit
GTCGGTGGGGGGAATTCGTGGAGAAAGAAATGGCTCCGTGGGCTGCAATA650.46076415963705963No Hit
GTCGGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA650.46076415963705963No Hit
GTCGGTGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCC650.46076415963705963No Hit
GTCGGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCC640.45367548025802795No Hit
GTCGGTGGGCTCTTTTACCGAGACCCGCCAACATGGGCCGCGTTCGCACC620.4394981214999646No Hit
GTCGGTGGCCAAAAAAAGAGTGCTGAATTTTTGCTGCACATGCTTAAAAA620.4394981214999646No Hit
GTCGGTGGGGAGGTTGCCTAAAACTCACTATGTAGCTTAGGCTGTGCTCA600.42532076274190117No Hit
GTCGGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC600.42532076274190117No Hit
GTCGGTGGGGAGCCTCTGTAAAGGGCAGGATAATAATTTAGACTTTGTTG590.41823208336286954No Hit
GTCGGTGGGGTAGCATTTCTGTCATGTAAGAGGTGCTGACAGCCACTTCC590.41823208336286954No Hit
GTCGGTGGGAGGATGGGTTTTTTGATCACAGGCAGTCCAGGGGACTTAAA580.4111434039838378No Hit
GTCGGTGGGGGAACAAGCCCGTGGAACCTGGAAGCCAGAGAACACCACCG580.4111434039838378No Hit
GTCGGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA570.4040547246048061No Hit
GTCGGTGGGCTCCAAATGAGCTGCTATAAAACACCTTTCAGGGGCTGAGA560.3969660452257744No Hit
GTCGGTGGGTGGGTATGGGGGACTTTTAGTATAGCATTGGAAATGTAAAT550.38987736584674276No Hit
GTCGGTGGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGT540.3827886864677111No Hit
GTCGGTGGGGCCAGGGACATCTTTGGAGTGAGCACCACACTTCAGGATGA530.3757000070886794No Hit
GTCGGTGGCAGCTTTACAGAGTCTCTTACTGGAGACAGCCCACTGCTATC530.3757000070886794No Hit
GTCGGTGGGCTCTCTTGCTTCTTGCTCTCTCTTGCTTCCCTCTCTTGCTT520.36861132770964766No Hit
GTCGGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCA520.36861132770964766No Hit
GTCGGTGGGAACAGAAATAGCTCCGAGCACCGCTGCTTCCTATCCTGTCA510.361522648330616No Hit
GTCGGTGGGAAGTAAGAAGCACCGTATGTTCCCTTTCGGACGTACCAACT510.361522648330616No Hit
GTCGGTGGGACTGGCTCCAGGCACACAGGCGGAGGGCAAGAGGGGTGGAC500.35443396895158436No Hit
GTCGGTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTT500.35443396895158436No Hit
GTCGGTGGGGTCGGTGCGGCAGGGATCCCTGCAGGGGGCGTCCTCACCCT500.35443396895158436No Hit
GTCGGTGGGGCAATCCCGGAAACTTTTGTTCACATCCCTGCTGAGTTATT500.35443396895158436No Hit
GTCGGTGGGGGGGCACAGTTATGCAGATGAGCGTCTGGGCGCAGGTTCCC490.3473452895725526No Hit
GTCGGTGGGGCATAGAGGGCTCCAACAGCGTCTTCTCTGGTTTCCTACTG480.34025661019352094No Hit
GTCGGTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTT480.34025661019352094No Hit
GTCGGTGGGAGACTTGCTTTGCTTACGTTCTAACACTGCCTTTCAGAGTT480.34025661019352094No Hit
GTCGGTGGGATTGTCGAGCTGGAGCAAGACTTGGGCCACTTAAAAGACGA470.33316793081448925No Hit
GTCGGTGGGGGAGGGGCTTGTGCTCACACAGCTGGTGGCACCTGGATGGG460.3260792514354576No Hit
GTCGGTGGGAGCTGCTAATGTTATTTTTGGTCAAACTGGAAAAATACCAG450.3189905720564259No Hit
GTCGGTGGGAGGAGCCCCCAGTGCTGCTTCCATCCCAAAGTTAGTGCTTA450.3189905720564259No Hit
GTCGGTGGGAAGGGAAGTGATCGTCGCCGGAGTCAGATCGTCTGGCTCGG450.3189905720564259No Hit
GTCGGTGGGAGCATGTGCTCTACCATCATGCTATGCTTCCAGCTCAGGGG450.3189905720564259No Hit
GTCGGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.3119018926773942No Hit
GTCGGTGGAGAAGTGGCAGTGCAGAGTGGGGCCACCTTCATCACTCACCT440.3119018926773942No Hit
GTCGGTGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCA430.30481321329836253No Hit
GTCGGTGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAA430.30481321329836253No Hit
GTCGGTGGGGCTGCTGTGTGGCCTTGGTGGTTAGATGCATCCCAGGTGTT420.29772453391933085No Hit
GTCGGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGC420.29772453391933085No Hit
GTCGGTGGGAAACGCTTTCTGAGTTCGGCTTCTCTGGATCGAATCCGGAC410.29063585454029917No Hit
GTCGGTGGGAAGTACTGGAAATCAGATCACAGCTTTCCAATGTGAGACAA410.29063585454029917No Hit
GTCGGTGGGGAGAGTGAAGGACCCGAAAATAATATAGAGAAGGACAGATG400.28354717516126743No Hit
GTCGGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCT400.28354717516126743No Hit
GTCGGTGGGAGTGGTGGCTCCATGCTTTGTAAACGGTATTTAATGGCTTG400.28354717516126743No Hit
GTCGGTGGGGGGCTAGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTT390.27645849578223575No Hit
GTCGGTGGGGGTGGTTGGTATAGCTTTGCTTTCTGGTGCTGTGATAAAAT390.27645849578223575No Hit
GTCGGTGGGGCTTTTCGGCTTTCGGCTCGGAGGAGGCAACGGTGCAACTT380.2693698164032041No Hit
GTCGGTGGGGACAGTGTGCGGAAAACCTCCCATCCTTATGTAGCTGACAG380.2693698164032041No Hit
GTCGGTGGGATATATATCATGTACATGCCTTTCTATACAACTTTTAAAGC380.2693698164032041No Hit
GTCGGTGGGGAATTTTGTATTGCTCGTTGGTGGGCAAGTCTTAGTGATGT370.2622811370241724No Hit
GTCGGTGGGAGCCTCAGCTGGTTCATCTGACATGGCAAGGTGAGCTGGCC370.2622811370241724No Hit
GTCGGTGGGGACAGTCTCACTCAGCGAGACGCCGCGCACGGTGCTTCCCC360.2551924576451407No Hit
GTCGGTGGGACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCT350.24810377826610902No Hit
GTCGGTGGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGT350.24810377826610902No Hit
GTCGGTGGGAAGCGCTTCATCCACCGCTGAGAGACATCCCGAGCCAACCT350.24810377826610902No Hit
GTCGGTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACA350.24810377826610902No Hit
GTCGGTGGGTTTGCGGGAGGAAGCATTTGTACAGTGGCTAATTTAAGGGG340.24101509888707734No Hit
GTCGGTGGGACTGAAAAAATATGTGTATAAATACCTTCTGATTTTTGTGA340.24101509888707734No Hit
GTCGGTAGGGGGACCAAGAATTCCTGAAAGTTGACCAAGGAACACTTTTT340.24101509888707734No Hit
GTCGGTGGGAAAACTGGCCTGTAAAAGCAGCTGTTCTGAGCCCGGTGCCC340.24101509888707734No Hit
GTCGGTGGGACACACATCTGGGGGTAGTGACATACAACTGTGTCTCAGCA340.24101509888707734No Hit
GTCGGTGGGAACCTGCATGCTGTAAATGTGTTTAAATAAAGGAAAATCTT330.23392641950804563No Hit
GTCGGTGGGCAGAGTGCAGTGCTGTCGGGGGCAGAGTGCAGTGCTGTCGG310.2197490607499823No Hit
GTCGGTGGGAAACAAACAAGACGAAGGGCAGCACTGGGAAGGTTTTGTAA310.2197490607499823No Hit
GTCGGTGGGGGAACAAATGCCAAGCTGGTGCCATTGGCTGTGTGGCTAGC310.2197490607499823No Hit
GTCGGTGGGAAGTGTTAGGCTTCAGGAGGGAGGGAGGTCTGTAATGTGAA300.21266038137095059No Hit
GTCGGTGGGGGAAGATGACTGTGTCTTTGGGCCTCCATTTGATTTTCCCC290.2055717019919189No Hit
GTCGGTGGGATTGAGATTGGTCCTGATGGAAGAGTGACGGGAGAAGCTGA290.2055717019919189No Hit
GTCGGTGGGAGATTTAGAACGTAATTACAGACTGGGCCAAGTGTTATGTT290.2055717019919189No Hit
GTCGGTGGGAGTGGTGGCTCCATGCTTTGTAAACGGTATTTAATGGCTTT290.2055717019919189No Hit
GTCGGTGGGGGGGCTGGAGAGATGGCTCGGCGGGGAAGAGCACTGACTGT270.19139434323385554No Hit
GTCGGTGGGTGGGAAAGAATTATTTAATAGTTGTAACCTTGGTCTCTAAC270.19139434323385554No Hit
GTCGGTGGGACTGGGCTGACGAGGTGGCTCAGTGGGTAAGAGCACCCGAC270.19139434323385554No Hit
GTCGGTGGGTGCTGTCGGTGGGGGGAATTCCTTCGATTCTGTTTTGCTAC260.18430566385482383No Hit
GTCGGTGGGGCCAGGCCTATGGCTTCTCCGGATGGGACATCGCGCTCCTG260.18430566385482383No Hit
GTCGGTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGATTGTT250.17721698447579218No Hit
GTCGGTGGGATGCCTCTTGTCCTCAGGATCACTGTCATTACAGCTTGCTT250.17721698447579218No Hit
GTCGGTGGGATATCAATATGTTATGCTGGCTAGTTTTATGTCAACTTGAC240.17012830509676047No Hit
GTCGGTGGGAGACGAAGCCCCCAAGGTGATTTGAAGCTCTTAACTTTGAA240.17012830509676047No Hit
GTCGGTGGGGGTCGAGACTCCTCTACCCTGTGCTAAGGTGTATGAGTTTC240.17012830509676047No Hit
GTCGGTGGGGGTTGTCTGTTTTGTTCTCTCCCTCCCAGTACATGTGTGAG240.17012830509676047No Hit
GTCGGTGGGGCAGATATGATTAGGGTTCCTCCCCTGACAAGACCTATCTT240.17012830509676047No Hit
GTCGGTGGGAGTCAAGGACAGAGGAAGGACTCCTGCCCCCTGAGCAGAGA230.1630396257177288No Hit
GTCGGTGGGAGAGCTCAAGTCTAAGTGGATCAAAGAACTCCACATAAAAC220.1559509463386971No Hit
GTCGGTGGGACATTACTATGTAAGTCATAGATTTCCTCATCATTACAAGG220.1559509463386971No Hit
GTCGGTGGGGTAAGTTTCATGCTACTTCTGTATCTTCATGCTTGTGAATC220.1559509463386971No Hit
GTCGGTGGGGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTT220.1559509463386971No Hit
GTCGGTGGGCAGTGCTACAATAGGGTTCTTTTTCTCGCTCGCTCTCACCC210.14886226695966542No Hit
GTCGGTGGGGGGAATTCGTGGAGAAAGAAATGGCTCCGTGGGCTGCCATA210.14886226695966542No Hit
GTCGGTGGGAGTGCTTGCCATCTATAGCCAAGTACCACCCTCTCTGCCCT210.14886226695966542No Hit
GTCGGTGGGAGATGATAGATATCTCCATCTGAAGACTTGGACTTTTTTCT210.14886226695966542No Hit
GTCGGTGGGGCCTGGTCCCCGACGCAACCATGGCTGAAGAACAACCTCAG210.14886226695966542No Hit
GTCGGTGGGAGTGATTAGCGATGTGTTTAGAGAACAAAAGCCTGACTGTA200.14177358758063371No Hit
GTCGGTGGGGGGAATTCGTGGAGAAAGAAATGGCTCCTCTGGCAGCATTT200.14177358758063371No Hit
GTCGGTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGGTGGGCTGCCATA190.13468490820160206No Hit
GTCGGTGGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGA180.12759622882257035No Hit
GTCGGTGGGCAGAGTGCAGTGCTAATAAAGGGGGAAAAAAAAAAAAAAAA180.12759622882257035No Hit
GTCGGTGGGTAGGACAACGCATTTCAGTAATAAAATACAGAATGGAAAAA180.12759622882257035No Hit
GTCGGTGGGAATATTACCCCTAACAGCCTGCCACCCCAGTCTTAATCAGT170.12050754944353867No Hit
GTCGGTGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGCT170.12050754944353867No Hit
GTCGGTGGGGTCCAGCAGTGTGTGTCTAGGGAAATGCTAGTGATTTTCCT160.11341887006450699No Hit
GTCGGTGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTA160.11341887006450699No Hit
GTCGGTGGGCTGCTGACATTAAGGAATTCATTTAAAACAAGCTTTTTCTT160.11341887006450699No Hit
GTCGGTGGGAACTAGGTCTCCCTTCCATAGATTGTGCCTTTCAGAAATGC150.10633019068547529No Hit
GTCGGTGGGGCCTGACAAAATCAGCCCATGAACCCCATGGAAGCCAACCC150.10633019068547529No Hit
GTCGGTGGGCGGTGGGTTGGGCAGGTCGAGGAGATGGTCCCTTAAAGTAC150.10633019068547529No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTGG407.355993E-944.00000423
ATGGGTT207.594592E-444.00000413
GATGGGT207.594592E-444.00000412
CTTTTAG207.594592E-444.00000423
ATCCCAA207.594592E-444.00000432
CAATGTA207.594592E-444.00000423
TTTTGAT207.594592E-444.00000420
GGATGGG207.594592E-444.00000411
AGGGGAC207.594592E-444.00000439
GGGTTTT207.594592E-444.00000415
GGTGAGG207.594592E-444.0000044
GAGAAAG407.355993E-944.00000421
CAGTCCA207.594592E-444.00000433
CTTTATA207.594592E-444.00000441
TAGTGGG407.355993E-944.00000428
TAGTGCT207.594592E-444.00000442
TATATCT207.594592E-444.00000444
TATACAT207.594592E-444.00000428
GAAATGG407.355993E-944.00000427
TTCGTGG407.355993E-944.00000415
Produced by FastQC (version 0.11.5)