FastQCFastQC Report
Fri 17 Jun 2016
SRR1527515_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527515_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12557
Sequences flagged as poor quality0
Sequence length52
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC4903.9022059409094525No Hit
GTCGGTGGGGGCTGGAGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTTT4373.480130604443737No Hit
GTCGGTGGGAAAACTGGCCTGTAAAAGCAGCTGTTCTGAGCCCAGTGCCCAT3572.843035756948316No Hit
GTCGGTGGGGTGTGAACGTCAGCTCGGCCATGGGCTGCAGCCAATCAGGGAG3012.397069363701521No Hit
GTCGGTGGGAGGAAGCAGTGGTTCTTCTGAACAGGGCAACAACACTGAGAAT2792.22186828064028No Hit
GTCGGTGGGAGTTGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCAGGG2662.1183403679222743No Hit
GTCGGTGGGGCTGGTGAGATGGCTCAGTGGTTAAAAGCGCCTACTGCTCTTC2091.6644102890817871No Hit
GTCGGTGAGCTACTCTAGTATTAATAAATATTAGCCCACCAACAGCTACCAT2061.640519232300709No Hit
GTCGGTGGGGGGGCTGGCGAGATAGCTCAGTGGTTAAGAGCACCAACTGCTC1981.5768097475511667No Hit
GTCGGTGGGAGCCGGGCGTGGTGGCGCACGCCTTTAATCCCAGCACTCGGGA1891.5051365772079317No Hit
GTCGGTGGGGTCACGAAATCCTTCGCTTAATTGCCGCACTTTGATGGTCAGT1861.4812455204268535No Hit
GTCGGTGGGCAGAAAGAAATAGCAATGTATACATCACCTTCTTTATATCTTA1811.4414270924583896No Hit
GTCGGTGGGGGGGCTGGCGAGATGGCTTAGTGGGTAAGAGCACTGACTGCTC1791.4254997212710043No Hit
GTCGGTGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTTCCCCAGTG1781.4175360356773115No Hit
GTCGGTGGGACTGACTGTTCTTCCAGAGGTCTTGAGTTCAAATCCCAGCAAC1641.3060444373656128No Hit
GTCGGTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGGGGGGCTGCAATAAA1230.9795333280242097No Hit
GTCGGTGGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG1230.9795333280242097No Hit
GTCGGTGGGAACCACCTCATGCCCACAAGGTACTCTGTGGATATCCCCTTGG1230.9795333280242097No Hit
GTCGGTGGGCATTTGTTTCGCCGCGAAACCGACACGCCTCCACGAAGCAACA1140.9078601576809748No Hit
GTCGGTGGGTGGGTAAGGGGGACTTTTGGTATAGCATTGGAAATGTAAATGA1100.8760054153062037No Hit
GTCGGTGGGAATCTATTTAAGGCGCTGCGCGGCCGGGCGTCTGATTCCCTGG1090.868041729712511No Hit
GTCGGTGGGGAGCCAATAGTGGAAGACATGCATCGCCAGTGGGCAAACCTGA1010.8043322449629688No Hit
GTCGGTGGGACATGCCCACCTGCAACTCCCTCCCTACCTCGCATAGCCAGTG940.7485864458071195No Hit
GTCGGTGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCC920.732659074619734No Hit
GTCGGTGAGGGGGCTGGCGAGATGGCTTAGTGGGTAAGAGCACTGACTGCTC900.7167317034323485No Hit
GTCGGTGGAGAATGAGGCCAGATTTCCTGGGCACAGACTTTGTAAGCCTTTG810.6450585330891137No Hit
GTCGGTGGGGATTTTGCTAATCCAGAGGCTGAGTGGAGCCGAGCTGGTCAGG760.6052401051206499No Hit
GTCGGTGGGATATGTAGAGAATTTTCTAGTTCTCCCAATTATAACTTTGTAA740.5893127339332643No Hit
GTCGGTGGGCTCTTTTACCGAGACCCGCCAACATGGGCCGCGTTCGCACCAA730.5813490483395716No Hit
GTCGGTGGGGTGGGGAGGAGCCTGGACAGGACCAGGACCCTGTGCAGTTGCT720.5733853627458788No Hit
GTCGGTGGGACTGGCTCCAGGCACACAGGCGGAGGGCAAGAGGGTTGGACAC700.5574579915584933No Hit
GTCGGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC690.5494943059648005No Hit
GTCGGTGGGACTTCTAATTCTCTCCGAGCCCTCTTGGTCACCTCTGTGCTTT670.533566934777415No Hit
GTCGGTGGGAGAGGACGACTATAAACAGGAAACCCAAAGCCTATCAGAAATG670.533566934777415No Hit
GTCGGTGGGCAAATCTACCCATTCAGTGCGGGGCTGAAAATAGCAAGCTGTT660.5256032491837221No Hit
GTCGGTGGGTATTGAGGATGGAATTTTTGAGGTCAAATCAACAGCTGGAGAC640.5096758779963367No Hit
GTCGGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG630.501712192402644No Hit
GTCGGTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGGGGGGGTGCAATAAA630.501712192402644No Hit
GTCGGTGGGGTAGCATTTCTGTCATGTAAGAGGTGCTGACAGCCACTTCCGT630.501712192402644No Hit
GTCGGTGGGAGCATGTGCTCTACCATCATGCTATGCTTCCAGCTCAGGGGGG610.4857848212152584No Hit
GTCGGTGGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACA610.4857848212152584No Hit
GTCGGTGGGTGGGTATGGGGGACTTTTGGTATAGCATTGGAAATGTAAATGA600.4778211356215657No Hit
GTCGGTGGGTGTTCAAACACTGATAGAAATTCTAACATGAATAAAGATAATA590.4698574500278729No Hit
GTCGGTGGGGTCGGTGCGGCAGGGATCCCTGCAGGGGGCGTCCTCACCCTCG580.4618937644341801No Hit
GTCGGTGGGGAGGTTGCCTAAAACTCACTATGTAGCTTAGGCTGTGCTCAAA580.4618937644341801No Hit
GTCGGTGGGGGAACAAGCCCGTGGAACCTGGAAGCCAGAGAACACCACCGCT560.44596639324679455No Hit
GTCGGTGGGGGGGCTGGAGAGATGGCTCGGCGGGGAAGAGCACTGACTGTTC560.44596639324679455No Hit
GTCGGTGGGAGGATGGGTTTTTTGATCACAGGCAGTCCAGGGGACTTAAACA550.43800270765310184No Hit
GTCGGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGG550.43800270765310184No Hit
GTCGGTGGGAGACTTGCTTTGCTTACGTTCTAACACTGCCTTTCAGAGTTCA550.43800270765310184No Hit
GTCGGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.4300390220594091No Hit
GTCGGTGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA530.4220753364657164No Hit
GTCGGTGGGGGTGGGCCTGGTCTTTTGTGTGTGAGGCCCTTGACCGTGTGGC520.41411165087202356No Hit
GTCGGTGGCCAAAAAAAGAGTGCTGAATTTTTGCTGCACATGCTTAAAAATG510.4061479652783308No Hit
GTCGGTGGGGAAATTATCCATAGTTTTACAGACAGCTCCAGGCTATGAGCCA510.4061479652783308No Hit
GTCGGTGGGACTCGGGAGGGGGGGGCGCCAAGATCCTCTATCCCTGTGGGGT500.3981842796846381No Hit
GTCGGTGGGGCCAGGGACATCTTTGGAGTGAGCACCACACTTCAGGATGACC490.3902205940909453No Hit
GTCGGTGGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGTTC480.3822569084972525No Hit
GTCGGTGGCAGCTTTACAGAGTCTCTTACTGGAGACAGCCCACTGCTATCTC460.366329537309867No Hit
GTCGGTGGGGAGCCTCTGTAAAGGGCAGGATAATAATTTAGACTTTGTTGAC440.3504021661224815No Hit
GTCGGTGGGAAACGCTTTCTGAGTTCGGCTTCTCTGGATCGAATCCGGACAC440.3504021661224815No Hit
GTCGGTGGGCTCCAAATGAGCTGCTATAAAACACCTTTCAGGGGCTGAGATG440.3504021661224815No Hit
GTCGGTGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCA440.3504021661224815No Hit
GTCGGTGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTC430.3424384805287887No Hit
GTCGGTGGGGACAGTGTGCGGAAAACCTCCCATCCTTATGTAGCTGACAGGG420.33447479493509596No Hit
GTCGGTGGGGCTGCTGTGTGGCCTTGGTGGTTAGATGCATCCCAGGTGTTCC410.3265111093414032No Hit
GTCGGTGGGAGCTGCTAATGTTATTTTTGGTCAAACTGGAAAAATACCAGCT400.31854742374771045No Hit
GTCGGTGGGTGGGTATGGGGGACTTTTAGTATAGCATTGGAAATGTAAATGA400.31854742374771045No Hit
GTCGGTGGGGGGGAATTCATCTGCGTGCGAAGAGGTGGCAGCCGTCTCGTTG390.3105837381540177No Hit
GTCGGTGGGAACAGAAATAGCTCCGAGCACCGCTGCTTCCTATCCTGTCAAC390.3105837381540177No Hit
GTCGGTGGGAGGAGCCCCCAGTGCTGCTTCCATCCCAAAGTTAGTGCTTACG390.3105837381540177No Hit
GTCGGTGGGGGGGCACAGTTATGCAGATGAGCGTCTGGGCGCAGGTTCCCGC380.3026200525603249No Hit
GTCGGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.3026200525603249No Hit
GTCGGTGGGATTGTCGAGCTGGAGCAAGACTTGGGCCACTTAAAAGACGAGA370.29465636696663217No Hit
GTCGGTGGGGCATAGAGGGCTCCAACAGCGTCTTCTCTGGTTTCCTACTGTT370.29465636696663217No Hit
GTCGGTGGGCAGTGCTACAATAGGGTTCTTTTTCTCGCTCGCTCTCACCCTC360.2866926813729394No Hit
GTCGGTGGGGCTTTCTGAGCCCCGGCGGTGCGCGCTACGGAGCTCGGCCAGC350.27872899577924665No Hit
GTCGGTGGGGGAGGGGCTTGTGCTCACACAGCTGGTGGCACCTGGATGGGCT350.27872899577924665No Hit
GTCGGTGGGCTCTCTTGCTTCTTGCTCTCTCTTGCTTCCCTCTCTTGCTTCT350.27872899577924665No Hit
GTCGGTGGGGCAATCCCGGAAACTTTTGTTCACATCCCTGCTGAGTTATTTG340.2707653101855539No Hit
GTCGGTGGGAGAGCTCAAGTCTAAGTGGATCAAAGAACTCCACATAAAACCA330.26280162459186107No Hit
GTCGGTGGGAAGTAAGAAGCACCGTATGTTCCCTTTCGGACGTACCAACTTC330.26280162459186107No Hit
GTCGGTGGGGAGAGTGAAGGACCCGAAAATAATATAGAGAAGGACAGATGGA330.26280162459186107No Hit
GTCGGTGGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCCG330.26280162459186107No Hit
GTCGGTGGGAAACAAACAAGACGAAGGGCAGCACTGGGAAGGTTTTGTAACC320.25483793899816837No Hit
GTCGGTGGGTTTGCGGGAGGAAGCATTTGTACAGTGGCTAATTTAAGGGGAG320.25483793899816837No Hit
GTCGGTGGGATGCCTCTTGTCCTCAGGATCACTGTCATTACAGCTTGCTTTA320.25483793899816837No Hit
GTCGGTGGGATATATATCATGTACATGCCTTTCTATACAACTTTTAAAGCTT320.25483793899816837No Hit
GTCGGTGGGGAGACGTTCTCGCCCAGAGTCGCCGCGGTTTCCTGCTTCAACA320.25483793899816837No Hit
GTCGGTGGGAGTCAAGGACAGAGGAAGGACTCCTGCCCCCTGAGCAGAGAAA310.24687425340447558No Hit
GTCGGTGGGAAGTACTGGAAATCAGATCACAGCTTTCCAATGTGAGACAAAC310.24687425340447558No Hit
GTCGGTGGGATTGAGATTGGTCCTGATGGAAGAGTGACGGGAGAAGCTGATG310.24687425340447558No Hit
GTCGGTGGGACACTCCGCCGCCGGCTTACACTGCGCTTCTTGCCGCTCCTCC310.24687425340447558No Hit
GTCGGTGGGTGCTGTCGGTGGGGGGAATTCCTTCGATTCTGTTTTGCTACCC310.24687425340447558No Hit
GTCGGTGGGGACAGTCTCACTCAGCGAGACGCCGCGCACGGTGCTTCCCCAG300.23891056781078285No Hit
GTCGGTGGGAAAACTGGCCTGTAAAAGCAGCTGTTCTGAGCCCGGTGCCCAT300.23891056781078285No Hit
GTCGGTGGGGCAGATATGATTAGGGTTCCTCCCCTGACAAGACCTATCTTTG300.23891056781078285No Hit
GTCGGTGGGAAGCGCTTCATCCACCGCTGAGAGACATCCCGAGCCAACCTTC300.23891056781078285No Hit
GTCGGTGGGTGGGAAAGAATTATTTAATAGTTGTAACCTTGGTCTCTAACTT300.23891056781078285No Hit
GTCGGTGGGACTGAAAAAATATGTGTATAAATACCTTCTGATTTTTGTGAAC300.23891056781078285No Hit
GTCGGTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAAC300.23891056781078285No Hit
GTCGGTGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCG290.23094688221709006No Hit
GTCGGTGGGAAGGGAAGTGATCGTCGCCGGAGTCAGATCGTCTGGCTCGGGA290.23094688221709006No Hit
GTCGGTGGGAGTGGTGGCTCCATGCTTTGTAAACGGTATTTAATGGCTTTGA290.23094688221709006No Hit
GTCGGTGGGGGGCTAGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGTTCT290.23094688221709006No Hit
GTCGGTGGGCAGAGTGCAGTGCTGTCGGGGGCAGAGTGCAGTGCTGTCGGTA280.22298319662339727No Hit
GTCGGTGGGGGAAGATGACTGTGTCTTTGGGCCTCCATTTGATTTTCCCCTC280.22298319662339727No Hit
GTCGGTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGGTGGGCTGCAATAAA270.21501951102970454No Hit
GTCGGTGGGATATCAATATGTTATGCTGGCTAGTTTTATGTCAACTTGACAC270.21501951102970454No Hit
GTCGGTGGGGAATTTTGTATTGCTCGTTGGTGGGCAAGTCTTAGTGATGTCA270.21501951102970454No Hit
GTCGGTGGGGTAAGTTTCATGCTACTTCTGTATCTTCATGCTTGTGAATCAT270.21501951102970454No Hit
GTCGGTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGATTGTTCT270.21501951102970454No Hit
GTCGGTGGAGAAGTGGCAGTGCAGAGTGGGGCCACCTTCATCACTCACCTCT260.20705582543601178No Hit
GTCGGTGGGGGTGGTTGGTATAGCTTTGCTTTCTGGTGCTGTGATAAAATAC250.19909213984231905No Hit
GTCGGTGGGACTGGGCTGACGAGGTGGCTCAGTGGGTAAGAGCACCCGACTG250.19909213984231905No Hit
GTCGGTGGGGCTTTTCGGCTTTCGGCTCGGAGGAGGCAACGGGGCAACTTTC250.19909213984231905No Hit
GTCGGTGGGAATATTACCCCTAACAGCCTGCCACCCCAGTCTTAATCAGTGG240.19112845424862626No Hit
GTCGGTGGGAGTGCTTGCCATCTATAGCCAAGTACCACCCTCTCTGCCCTCA240.19112845424862626No Hit
GTCGGTGGGGCCAGGCCTATGGCTTCTCCGGATGGGACATCGCGCTCCTGGT240.19112845424862626No Hit
GTCGGTGGGGCTTTTCGGCTTTCGGCTCGGAGGAGGCAACGGTGCAACTTTC240.19112845424862626No Hit
GTCGGTGGGAGATTTAGAACGTAATTACAGACTGGGCCAAGTGTTATGTTAA230.1831647686549335No Hit
GTCGGTGGGGGGAATTCGTGGAGAAAGAAATGGCTCGGGGGGCTGCCATAAA220.17520108306124074No Hit
GTCGGTGGGGGTTGTCTGTTTTGTTCTCTCCCTCCCAGTACATGTGTGAGGC220.17520108306124074No Hit
GTCGGTGGGAGATGATAGATATCTCCATCTGAAGACTTGGACTTTTTTCTCT220.17520108306124074No Hit
GTCGGTGGGGGGGCGGGAGGCTAACATTTCGATTAATTCCTTGCGGTCTTGT220.17520108306124074No Hit
GTCGGTAGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC210.16723739746754798No Hit
GTCGGTGGGGGGCTGGAGAGATGGCTCAGCGGGTAAGAGCACTGACTGCTCT210.16723739746754798No Hit
GTCGGTGGGGGAACAAATGCCAAGCTGGTGCCATTGGCTGTGTGGCTAGCTG200.15927371187385522No Hit
GTCGGTGGGGGTCGAGACTCCTCTACCCTGTGCTAAGGTGTATGAGTTTCGA200.15927371187385522No Hit
GTCGGTGGGTGAACTAAAGTTTTCAATTTTTTAATTCAATATGATTGTTTCT190.15131002628016246No Hit
GTCGGTAGGGGGACCAAGAATTCCTGAAAGTTGACCAAGGAACACTTTTTGA190.15131002628016246No Hit
GTCGGTGGGACACACATCTGGGGGTAGTGACATACAACTGTGTCTCAGCACC190.15131002628016246No Hit
GTCGGTGGGAGCCTCAGCTGGTTCATCTGACATGGCAAGGTGAGCTGGCCAC190.15131002628016246No Hit
GTCGGTGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTATC180.1433463406864697No Hit
GTCGGTGGGGTCCAGCAGTGTGTGTCTAGGGAAATGCTAGTGATTTTCCTGA180.1433463406864697No Hit
GTCGGTGGGATTGTTATTTTTCTGTTCTGTTACTTTCTGTGAGTGATGATGT180.1433463406864697No Hit
GTCGGTGGGATGACATATTATTTGTGCTCACAAAAATAGTTCTAAAAATAGT170.13538265509277694No Hit
GTCGGTGGGAAGTTTGCTGAGCTTAAAGAAAAGATCGATCGTCGTTCTGGTA160.12741896949908418No Hit
GTCGGTGGGAGGTTTTAAGTTTTAGCATTGAGTATCTTATCTGCCTGCTATC160.12741896949908418No Hit
GTCGGTGGGAGTGGTGGCTCCATGCTTTGTAAACGGTATTTAATGGCTTTGG160.12741896949908418No Hit
GTCGGTGGGAATAAAAGAAACCCTGTTTGAACTAATAATACAGACATTGATC150.11945528390539142No Hit
GTCGGTGGGCTGCTGACATTAAGGAATTCATTTAAAACAAGCTTTTTCTTTG150.11945528390539142No Hit
GTCGGTGGGCGGTGGGTTGGGCAGGTCGAGGAGATGGTCCCTTAAAGTACTT150.11945528390539142No Hit
GTCGGTGGGGGAGTGTTAAGAATAAACAATTTCACTGTGAAAAAAAAAAAAA150.11945528390539142No Hit
GTCGGTGGGAACCTGCATGCTGTAAATGTGTTTAAATAAAGGAAAATCTTTT140.11149159831169864No Hit
GTCGGTGGGAAGTGTTAGGCTTCAGGAGGGAGGGGGGGCTGTAATGTGAAAC140.11149159831169864No Hit
GTCGGTGGGGCCTGACAAAATCAGCCCATGAACCCCATGGAAGCCAACCCCA140.11149159831169864No Hit
GTCGGTGGGGGGAATTCGTGGAGAAAGAAATGGCTCCGGGGGCTGCAATAAA140.11149159831169864No Hit
GTCGGTGGGAGCTATCCCCAGGACACACTGTATTCACAAGCCAGAATGGTGC140.11149159831169864No Hit
GTCGGTGGGGGCCGTGTCCATGGAGAGAAGTTGAGTCCCCACGCTCCAGCCG140.11149159831169864No Hit
GTCGGTGGGGCCCTTTTCCCTTTCTGCCACCGCCATGCCATCCAGACTGAGG140.11149159831169864No Hit
GTCGGTGGGAAGCAGTGGCATCAACGGCAGAGTACAATGCTAATAAAGGGGG130.10352791271800589No Hit
GTCGGTGGGAGTGATTAGCGATGTGTTTAGAGAACAAAAGCCTGACTGTACT130.10352791271800589No Hit
GTCGGTGGGAAGTGTTAGGCTTCAGGAGGGAGGGGGGTCTGTAATGTGAAAC130.10352791271800589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTAT359.1589754E-846.00000410
ATCTATT359.1589754E-846.00000411
TCGGTGA359.1589754E-846.0000042
AGGCGCT359.1589754E-846.00000420
TCTATTT359.1589754E-846.00000412
AAAAGCG359.1589754E-846.00000433
GTGGGCT359.1589754E-846.0000045
GTGGGAC700.046.0000045
TTAAGGC359.1589754E-846.00000417
CGGTGAG359.1589754E-846.0000043
GAATCTA359.1589754E-846.0000049
AAGGCGC359.1589754E-846.00000419
TGGGCAG359.1589754E-846.0000046
TGGGAGC359.1589754E-846.0000046
GGAATCT359.1589754E-846.0000048
TTTAAGG359.1589754E-846.00000416
CTATTTA359.1589754E-846.00000413
TAAGGCG359.1589754E-846.00000418
ATTTAAG359.1589754E-846.00000415
TGATGGT206.0747133E-446.042
Produced by FastQC (version 0.11.5)