SRR1527513_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1527513_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	680359
Sequences flagged as poor quality	0
Sequence length	50
%GC	58

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5465	0.803252400570875	No Hit
CTGTGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	3265	0.4798937031772932	No Hit
CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	3039	0.446675946081407	No Hit
CTGTGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	2881	0.4234529123595043	No Hit
CTGTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2456	0.3609858912721078	No Hit
CTGTGTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	1939	0.2849965973846161	No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	1447	0.21268183414932412	No Hit
CTGTGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1437	0.21121202188844418	No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1412	0.20753749123624438	No Hit
CTGTGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1367	0.20092333606228477	No Hit
CTGTGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	1325	0.19475012456658913	No Hit
CTGTGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	1220	0.17931709582734998	No Hit
CTGTGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	1153	0.1694693536794545	No Hit
CTGTGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	1046	0.1537423624880394	No Hit
CTGTGTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	1045	0.1535953812619514	No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	1030	0.15139066287063152	No Hit
CTGTGTGGGAGACCGACCCTCGGCTCCGGAGCGGCAGTGCCGTCCCTCTC	1013	0.14889198202713566	No Hit
CTGTGTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	1010	0.1484510383488717	No Hit
CTGTGTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	1000	0.14698122608799177	No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAA	973	0.14301273298361603	No Hit
CTGTGTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	873	0.1283146103748168	No Hit
CTGTGTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	845	0.12419913604435305	No Hit
CTGTGTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	824	0.12111253029650523	No Hit
CTGTGTGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACT	807	0.11861384945300937	No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAGTGCAGTGCTCTGTGTGG	776	0.11405743144428163	No Hit
CTGTGTGGGGCTCAGCCCGTAGCCCGTCGGTTCCGGAGTAAGTTCCAGGT	774	0.11376346899210565	No Hit
CTGTGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	766	0.11258761918340171	No Hit
CTGTGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	751	0.11038290079208182	No Hit
CTGTGTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	730	0.107296295044234	No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAA	722	0.10612044523553006	No Hit
CTGTGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	714	0.10494459542682615	No Hit
CTGTGTGGGACCTCTGAAGACATTTAAGAGGTTCTCCTGCCCCCGGGCGT	697	0.10244591458333029	No Hit
CTGTGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	686	0.10082912109636237	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATTG	40	8.303687E-9	44.000004	39
TAATATC	40	8.303687E-9	44.000004	39
ACTATAC	40	8.303687E-9	44.000004	41
ACCGGTA	20	7.854213E-4	44.000004	13
TAGGTCG	20	7.854213E-4	44.000004	36
GACCGTT	40	8.303687E-9	44.000004	43
GAATCGA	20	7.854213E-4	44.000004	39
TGTATTC	20	7.854213E-4	44.000004	26
ATATCCA	20	7.854213E-4	44.000004	42
TAACCAC	20	7.854213E-4	44.000004	31
GTTAACC	20	7.854213E-4	44.000004	35
CAAGGCG	20	7.854213E-4	44.000004	37
TTGACCG	20	7.854213E-4	44.000004	12
CGGTGAT	20	7.854213E-4	44.000004	17
CGTACCA	20	7.854213E-4	44.000004	43
TTAGTTA	20	7.854213E-4	44.000004	17
ACGTCCC	20	7.854213E-4	44.000004	19
CTACATG	20	7.854213E-4	44.000004	42
TTACGTC	20	7.854213E-4	44.000004	17
CGTAACT	20	7.854213E-4	44.000004	41

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content