##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527513_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	680359
Sequences flagged as poor quality	0
Sequence length	50
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22647308259316	31.0	31.0	33.0	30.0	34.0
2	31.002400203422017	31.0	31.0	34.0	27.0	34.0
3	32.09172804357699	34.0	31.0	34.0	30.0	34.0
4	34.4877307421523	35.0	35.0	37.0	32.0	37.0
5	35.34670225572088	35.0	35.0	37.0	33.0	37.0
6	34.77653562310486	35.0	35.0	37.0	32.0	37.0
7	35.9627549573093	37.0	35.0	37.0	35.0	37.0
8	36.48809084615622	37.0	37.0	37.0	35.0	37.0
9	38.576147298705536	39.0	39.0	39.0	39.0	39.0
10	37.58348313169959	39.0	37.0	39.0	35.0	39.0
11	37.21033601378096	39.0	37.0	39.0	34.0	39.0
12	37.05600278676405	39.0	37.0	39.0	34.0	39.0
13	36.963910229746354	39.0	37.0	39.0	34.0	39.0
14	37.810466239147274	40.0	38.0	40.0	34.0	40.0
15	37.89847565770424	40.0	38.0	40.0	34.0	40.0
16	37.99772914005694	40.0	38.0	40.0	34.0	40.0
17	37.961695222669206	40.0	38.0	40.0	34.0	40.0
18	37.79461284410142	40.0	37.0	40.0	34.0	40.0
19	37.788868817785904	40.0	37.0	40.0	34.0	40.0
20	37.531685477813916	39.0	37.0	40.0	33.0	40.0
21	37.48327721100184	39.0	36.0	40.0	33.0	40.0
22	37.30479937797545	39.0	36.0	40.0	33.0	40.0
23	37.14166491514039	38.0	36.0	40.0	33.0	40.0
24	36.95515161848377	38.0	36.0	40.0	33.0	40.0
25	36.48778806483048	38.0	35.0	40.0	32.0	40.0
26	36.055583890269695	38.0	35.0	39.0	31.0	40.0
27	35.770174275639775	38.0	34.0	39.0	31.0	40.0
28	35.774840929568064	38.0	34.0	39.0	31.0	40.0
29	35.43250107663748	37.0	34.0	39.0	30.0	40.0
30	35.11456598648655	37.0	34.0	38.0	30.0	40.0
31	34.959584572262585	36.0	33.0	38.0	29.0	40.0
32	34.84867400886885	36.0	33.0	38.0	29.0	40.0
33	34.27478581160828	36.0	33.0	38.0	28.0	39.0
34	33.83043510852359	35.0	32.0	38.0	27.0	39.0
35	33.683478869244034	35.0	32.0	38.0	27.0	39.0
36	33.39385824248669	35.0	32.0	38.0	26.0	39.0
37	33.10291478469455	34.0	31.0	38.0	26.0	39.0
38	32.62664563855259	34.0	31.0	37.0	25.0	38.0
39	31.911906802144163	34.0	30.0	36.0	23.0	38.0
40	31.625069999808925	33.0	30.0	36.0	23.0	38.0
41	31.215572954866474	33.0	29.0	36.0	23.0	38.0
42	30.738457196862246	33.0	29.0	35.0	22.0	38.0
43	30.258103442447297	32.0	28.0	35.0	21.0	37.0
44	29.786866933486586	31.0	27.0	34.0	21.0	37.0
45	29.305853233366502	31.0	26.0	34.0	20.0	37.0
46	29.143777035359275	31.0	26.0	34.0	20.0	36.0
47	28.72169398802691	31.0	26.0	34.0	19.0	36.0
48	28.15743600069963	30.0	24.0	33.0	18.0	36.0
49	27.453157818151887	29.0	24.0	33.0	17.0	35.0
50	30.52734365239528	32.0	28.0	35.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	21.0
13	33.0
14	54.0
15	79.0
16	149.0
17	219.0
18	367.0
19	556.0
20	839.0
21	1135.0
22	1800.0
23	2385.0
24	3326.0
25	4604.0
26	6167.0
27	8509.0
28	11235.0
29	14942.0
30	20100.0
31	27347.0
32	41871.0
33	65768.0
34	92954.0
35	160993.0
36	176416.0
37	37884.0
38	601.0
39	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.67634734015424	0.14580537627928786	0.1412489582705601	0.03659832529590995
8	99.37871035732606	0.3058679314891109	0.2589809203670415	0.05644079081778884
9	98.61029250733804	0.8192733542144661	0.2605977138540094	0.3098364245934867
10	64.96482004353584	22.190049664956295	4.75219700187695	8.092933289630915
11	35.55049613512866	28.323429248382105	19.20001058264828	16.926064033840955
12	37.42362488039403	15.362918694395164	25.0051810882196	22.208275336991207
13	24.40241108003275	15.337049998603677	31.375200445647078	28.885338475716498
14	31.177657677784815	16.227315285018644	28.485255578304987	24.109771458891554
15	26.456620695838524	14.058754275316415	24.920519901992918	34.56410512685215
16	31.026854939818538	20.694221727058803	27.124062443504094	21.15486088961857
17	38.88285449299561	14.868767812287336	21.334618929124183	24.91375876559287
18	29.08170539377005	15.578099209387986	30.493607051571303	24.84658834527066
19	37.32999783937598	16.963397265267307	20.80357575926827	24.90302913608845
20	27.810905713013277	17.200477982947238	25.316487325073965	29.672128978965517
21	32.32646294088856	16.679723498917483	29.07612010717871	21.917693453015247
22	31.600669646466056	15.546792208231242	24.82027870580091	28.032259439501793
23	33.773052168046576	16.828909443396796	28.143818190102575	21.254220198454053
24	39.17431826432809	16.46189732185508	21.62975723110887	22.73402718270795
25	30.03267392655936	16.191304884627087	28.38354456985209	25.392476618961464
26	35.462601361928044	16.493057341785732	22.753869648229834	25.290471648056396
27	30.901773916417657	15.297218086333833	26.283182849054693	27.51782514819382
28	37.90734009544961	18.728053865679737	18.60267887982668	24.761927159043974
29	37.5757504493951	19.642424073173135	19.06449389219515	23.717331585236618
30	35.72804945624296	19.901551974766264	20.40555059902199	23.964847969968798
31	36.67534345838006	20.895439025573264	18.838730728923995	23.590486787122682
32	36.82232468446806	20.47198611321376	20.101005498567666	22.60468370375052
33	34.15373354361447	20.86486693054696	21.29728569769783	23.68411382814073
34	30.219781027369375	22.802520434064956	24.387272013745683	22.590426524819986
35	29.961388031906683	26.24070527471526	22.01543596836376	21.782470725014296
36	30.21419574077803	26.270101519932858	20.614263940066934	22.901438799222177
37	31.515714497787194	25.89074297539975	19.339348784979695	23.254193741833358
38	30.849889543608594	26.198521662828007	19.571726103424812	23.37986269013859
39	31.816732048815403	23.865341679907225	20.8435546527642	23.474371618513167
40	30.18200685226476	23.509647112774285	19.124315251212963	27.184030783747993
41	26.989721602859667	24.83424192227927	20.603240348110337	27.57279612675073
42	26.640641190900688	21.832003398205945	22.34996523894003	29.177390171953338
43	27.314844074966306	20.950850947808437	24.316721025223448	27.417583952001813
44	27.84309460152655	21.55509076825617	23.361343055651503	27.240471574565785
45	29.266754757414837	21.42486540194221	22.29793388490488	27.01044595573807
46	28.00668470616248	23.910170953864064	22.168884368399624	25.914259971573834
47	27.71536791605608	22.61423748344624	22.304254077626666	27.366140522871014
48	28.606956033505842	23.740260656506347	23.283589987050952	24.369193322936862
49	30.396452461127137	22.739024544394944	21.264067940601947	25.60045505387597
50	26.636378735344135	22.875423122204598	22.865428398830616	27.622769743620644
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	2.0
7	2.0
8	2.0
9	14.5
10	27.0
11	61.5
12	96.0
13	172.5
14	249.0
15	370.0
16	491.0
17	501.0
18	511.0
19	543.0
20	575.0
21	842.0
22	1109.0
23	1031.0
24	953.0
25	1190.5
26	1428.0
27	1758.5
28	2089.0
29	2388.0
30	2687.0
31	3466.0
32	4245.0
33	5020.0
34	5795.0
35	6257.5
36	6720.0
37	7874.0
38	9028.0
39	10245.5
40	11463.0
41	11285.5
42	11108.0
43	11719.5
44	12331.0
45	13176.5
46	14022.0
47	15254.5
48	16487.0
49	18354.5
50	20222.0
51	22639.5
52	25057.0
53	28513.5
54	31970.0
55	38089.5
56	44209.0
57	52802.5
58	61396.0
59	66826.0
60	72256.0
61	75414.0
62	78572.0
63	77774.5
64	76977.0
65	67469.5
66	57962.0
67	49259.0
68	40556.0
69	31840.0
70	23124.0
71	19400.5
72	15677.0
73	13692.5
74	11708.0
75	12700.5
76	13693.0
77	8810.0
78	3927.0
79	2554.5
80	1182.0
81	746.0
82	310.0
83	220.5
84	131.0
85	70.5
86	10.0
87	5.0
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	680359.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.893841339645688
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.7445836154367	10.107017030714667
2	9.391926201760326	2.609798650418382
3	3.4931448882870684	1.4559960256276465
4	1.674636086662153	0.9306851235891639
5	0.9700829383886256	0.6739089216134423
6	0.6294431279620853	0.5247229771341306
7	0.4898019634394042	0.47636615375118135
8	0.3924763033175355	0.43624027902915957
9	0.3141926201760325	0.392880817333202
>10	6.569482058226134	24.521906816842286
>50	2.1919431279620856	20.932478294547437
>100	1.080103249830738	28.451743858756924
>500	0.03914184157075152	3.5550349153902574
>1k	0.017984089370345294	4.127967734681249
>5k	0.0010578876100203114	0.803252400570875
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5465	0.803252400570875	No Hit
CTGTGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGC	3265	0.4798937031772932	No Hit
CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGC	3039	0.446675946081407	No Hit
CTGTGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCC	2881	0.4234529123595043	No Hit
CTGTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	2456	0.3609858912721078	No Hit
CTGTGTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCG	1939	0.2849965973846161	No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGC	1447	0.21268183414932412	No Hit
CTGTGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1437	0.21121202188844418	No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	1412	0.20753749123624438	No Hit
CTGTGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCG	1367	0.20092333606228477	No Hit
CTGTGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGA	1325	0.19475012456658913	No Hit
CTGTGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTT	1220	0.17931709582734998	No Hit
CTGTGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTG	1153	0.1694693536794545	No Hit
CTGTGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTC	1046	0.1537423624880394	No Hit
CTGTGTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTT	1045	0.1535953812619514	No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGT	1030	0.15139066287063152	No Hit
CTGTGTGGGAGACCGACCCTCGGCTCCGGAGCGGCAGTGCCGTCCCTCTC	1013	0.14889198202713566	No Hit
CTGTGTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGC	1010	0.1484510383488717	No Hit
CTGTGTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCG	1000	0.14698122608799177	No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAA	973	0.14301273298361603	No Hit
CTGTGTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGC	873	0.1283146103748168	No Hit
CTGTGTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAA	845	0.12419913604435305	No Hit
CTGTGTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAG	824	0.12111253029650523	No Hit
CTGTGTGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACT	807	0.11861384945300937	No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAGTGCAGTGCTCTGTGTGG	776	0.11405743144428163	No Hit
CTGTGTGGGGCTCAGCCCGTAGCCCGTCGGTTCCGGAGTAAGTTCCAGGT	774	0.11376346899210565	No Hit
CTGTGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCA	766	0.11258761918340171	No Hit
CTGTGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCG	751	0.11038290079208182	No Hit
CTGTGTGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGAGCACTGACTGCT	730	0.107296295044234	No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAA	722	0.10612044523553006	No Hit
CTGTGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAG	714	0.10494459542682615	No Hit
CTGTGTGGGACCTCTGAAGACATTTAAGAGGTTCTCCTGCCCCCGGGCGT	697	0.10244591458333029	No Hit
CTGTGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCC	686	0.10082912109636237	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	1.4698122608799177E-4	0.0	0.0	0.0	0.0
8	0.0014698122608799177	0.0	0.0	0.0	0.0
9	0.0024986808434958605	0.0	0.0	0.0	0.0
10	0.019254540617526923	0.0	0.0	0.0	0.0
11	0.06525966438306835	0.0	0.0	0.0	0.0
12	0.07775306860054765	0.0	0.0	0.0	0.0
13	0.08907062300932302	0.0	0.0	0.0	0.0
14	0.10229893335724229	0.0	0.0	0.0	0.0
15	0.11376346899210564	0.0	0.0	0.0	0.0
16	0.1341938594183365	0.0	0.0	0.0	0.0
17	0.1447765076966719	0.0	0.0	0.0	0.0
18	0.1537423624880394	0.0	0.0	0.0	0.0
19	0.16403104831419882	0.0	0.0	0.0	0.0
20	0.1732908655577423	0.0	0.0	0.0	0.0
21	0.18549030732304564	0.0	0.0	0.0	0.0
22	0.19548503069702908	0.0	0.0	0.0	0.0
23	0.20636164142754046	0.0	0.0	0.0	0.0
24	0.21944297054937173	0.0	0.0	0.0	0.0
25	0.23281826212337897	0.0	0.0	0.0	0.0
26	0.250456009253938	0.0	0.0	0.0	0.0
27	0.269122624967113	0.0	0.0	0.0	0.0
28	0.2920516962368397	0.0	0.0	0.0	0.0
29	0.31174718053263056	0.0	0.0	0.0	0.0
30	0.33511719548062124	0.0	0.0	0.0	0.0
31	0.3605449475938438	0.0	0.0	0.0	0.0
32	0.38861836177665027	0.0	0.0	0.0	0.0
33	0.4131642265333449	0.0	0.0	0.0	0.0
34	0.4431483966552952	0.0	0.0	0.0	0.0
35	0.48033464685555716	0.0	0.0	0.0	0.0
36	0.5117886292383874	0.0	0.0	0.0	0.0
37	0.5432426116212177	0.0	0.0	0.0	0.0
38	0.5763133874910158	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATTG	40	8.303687E-9	44.000004	39
TAATATC	40	8.303687E-9	44.000004	39
ACTATAC	40	8.303687E-9	44.000004	41
ACCGGTA	20	7.854213E-4	44.000004	13
TAGGTCG	20	7.854213E-4	44.000004	36
GACCGTT	40	8.303687E-9	44.000004	43
GAATCGA	20	7.854213E-4	44.000004	39
TGTATTC	20	7.854213E-4	44.000004	26
ATATCCA	20	7.854213E-4	44.000004	42
TAACCAC	20	7.854213E-4	44.000004	31
GTTAACC	20	7.854213E-4	44.000004	35
CAAGGCG	20	7.854213E-4	44.000004	37
TTGACCG	20	7.854213E-4	44.000004	12
CGGTGAT	20	7.854213E-4	44.000004	17
CGTACCA	20	7.854213E-4	44.000004	43
TTAGTTA	20	7.854213E-4	44.000004	17
ACGTCCC	20	7.854213E-4	44.000004	19
CTACATG	20	7.854213E-4	44.000004	42
TTACGTC	20	7.854213E-4	44.000004	17
CGTAACT	20	7.854213E-4	44.000004	41
>>END_MODULE