FastQCFastQC Report
Fri 17 Jun 2016
SRR1527512_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527512_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574470
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC47210.8218009643671559No Hit
CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA31080.5410204188208262No Hit
CTGTGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT26240.45676884780754434No Hit
CTGTGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC24800.4317022646961547No Hit
CTGTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG20340.35406548644837854No Hit
CTGTGTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC16560.2882657057809807No Hit
CTGTGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT12520.2179400142740265No Hit
CTGTGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG12160.21167336849617907No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC11760.20471042874301532No Hit
CTGTGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG11510.20035859139728793No Hit
CTGTGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG11220.1953104600762442No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT10440.1817327275575748No Hit
CTGTGTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC10180.17720681671801836No Hit
CTGTGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT9980.17372534684143645No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC8880.15457726252023604No Hit
CTGTGTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA8740.15214023360662873No Hit
CTGTGTGGGAGACCGACCCTCGGCTCCGGAGCGGCAGTGCCGTCCCTCTCGG8610.14987727818685048No Hit
CTGTGTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG8560.149006910717705No Hit
CTGTGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC8380.14587358782878132No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAA7680.1336884432607447No Hit
CTGTGTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG7630.1328180757915992No Hit
CTGTGTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA7280.12672550350758088No Hit
CTGTGTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA7200.12533291555694814No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAA6950.12098107821122078No Hit
CTGTGTGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT6770.11784775532229708No Hit
CTGTGTGGGGCTCAGCCCGTAGCCCGTCGGTTCCGGAGTAAGTTCCAGGTGG6560.11419221195188609No Hit
CTGTGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT6450.11227740351976605No Hit
CTGTGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT6350.1105366685814751No Hit
CTGTGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC6350.1105366685814751No Hit
CTGTGTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA6250.10879593364318414No Hit
CTGTGTGGGACCTCTGAAGACATTTAAGAGGTTCTCCTGCCCCCGGGCGTGG6130.10670705171723502No Hit
CTGTGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC5980.1040959493097986No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTAGT351.0183794E-746.00000421
GTACCGA351.0183794E-746.00000439
GTCGTAC351.0183794E-746.00000431
TACCGAC351.0183794E-746.00000440
TGCCGAT351.0183794E-746.00000418
TCACGGT206.308805E-446.026
TAATATC501.6370905E-1146.039
CGGAAAT206.308805E-446.039
GTATTGC206.308805E-446.09
GCGAATT253.415006E-546.026
CTATCCA253.415006E-546.038
CGCATGA405.6024874E-946.043
ATTACGG206.308805E-446.026
GGTCCTA206.308805E-446.09
CCTACTT206.308805E-446.016
CACGCCA405.6024874E-946.029
TATATTT206.308805E-446.043
ATTAATC405.6024874E-946.035
ATTGTAC206.308805E-446.023
GTCTATT405.6024874E-946.016
Produced by FastQC (version 0.11.5)