##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1527512_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	574470
Sequences flagged as poor quality	0
Sequence length	52
%GC	58
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.611826553170747	31.0	31.0	33.0	30.0	33.0
2	32.06246627326057	33.0	31.0	34.0	31.0	34.0
3	32.740430309676746	34.0	31.0	34.0	31.0	34.0
4	34.810352150678014	35.0	35.0	37.0	33.0	37.0
5	35.6710776890003	37.0	35.0	37.0	35.0	37.0
6	34.84497188713075	35.0	35.0	37.0	33.0	37.0
7	36.03771998537783	37.0	35.0	37.0	35.0	37.0
8	36.561787386634634	37.0	37.0	37.0	35.0	37.0
9	38.67950980904138	39.0	39.0	39.0	39.0	39.0
10	37.813384510940516	39.0	38.0	39.0	35.0	39.0
11	37.510496631677896	39.0	37.0	39.0	35.0	39.0
12	37.43168659808171	39.0	37.0	39.0	35.0	39.0
13	37.319113269622434	39.0	37.0	39.0	35.0	39.0
14	38.126683725869064	40.0	38.0	40.0	35.0	40.0
15	38.30529531568228	40.0	38.0	40.0	35.0	40.0
16	38.370708653193375	40.0	38.0	40.0	35.0	40.0
17	38.38737619022751	40.0	38.0	40.0	35.0	40.0
18	38.270069803471024	40.0	38.0	40.0	35.0	40.0
19	38.240837641652305	40.0	38.0	40.0	34.0	40.0
20	38.1133653628562	40.0	38.0	40.0	34.0	40.0
21	38.11836997580379	40.0	38.0	40.0	34.0	40.0
22	38.113669991470395	40.0	38.0	40.0	34.0	40.0
23	37.99499886852229	40.0	38.0	40.0	34.0	40.0
24	37.91074729750901	40.0	37.0	40.0	34.0	40.0
25	37.783910386965374	40.0	37.0	40.0	34.0	40.0
26	37.76933521332707	40.0	37.0	40.0	34.0	40.0
27	37.652093233763296	40.0	37.0	40.0	34.0	40.0
28	37.62861942312044	40.0	37.0	40.0	34.0	40.0
29	37.61210855223075	40.0	37.0	40.0	34.0	40.0
30	37.41624801991401	40.0	36.0	40.0	33.0	40.0
31	37.3332045189479	39.0	36.0	40.0	33.0	40.0
32	37.19732623113479	39.0	35.0	40.0	33.0	40.0
33	36.92209340783679	39.0	35.0	40.0	32.0	40.0
34	36.540398976447854	39.0	35.0	40.0	31.0	40.0
35	36.50642331192229	39.0	35.0	40.0	31.0	40.0
36	36.39835500548332	38.0	35.0	40.0	31.0	40.0
37	36.22145107664456	38.0	35.0	40.0	31.0	40.0
38	36.38449701463958	38.0	35.0	40.0	31.0	40.0
39	36.283797239194385	38.0	35.0	40.0	31.0	40.0
40	36.02258429508939	38.0	35.0	40.0	30.0	40.0
41	35.832114818876526	38.0	35.0	40.0	30.0	40.0
42	35.65514822357999	38.0	35.0	40.0	30.0	40.0
43	35.59732971260466	37.0	35.0	40.0	30.0	40.0
44	35.381922467665845	37.0	34.0	40.0	30.0	40.0
45	35.26353856598256	37.0	34.0	40.0	29.0	40.0
46	35.08338294427907	36.0	34.0	40.0	29.0	40.0
47	34.93997423712291	36.0	34.0	40.0	29.0	40.0
48	34.625144916183615	35.0	34.0	39.0	28.0	40.0
49	34.406881125211065	35.0	33.0	39.0	27.0	40.0
50	34.67665848521246	35.0	34.0	39.0	28.0	40.0
51	34.6965951224607	35.0	34.0	39.0	29.0	40.0
52	34.246260030985084	35.0	34.0	39.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	11.0
13	14.0
14	25.0
15	44.0
16	69.0
17	107.0
18	167.0
19	240.0
20	296.0
21	562.0
22	825.0
23	1087.0
24	1571.0
25	2232.0
26	3009.0
27	3922.0
28	5077.0
29	6317.0
30	7820.0
31	10579.0
32	14956.0
33	21126.0
34	41827.0
35	47825.0
36	75535.0
37	119470.0
38	180994.0
39	28757.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	100.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	100.0	0.0
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	99.65028635089735	0.14517729385346492	0.17302905286612008	0.031507302383066135
8	99.39857607882048	0.2752101937437986	0.2825212804846206	0.043692446951102755
9	98.67547478545443	0.770623357181402	0.25797691785471827	0.2959249395094609
10	65.36877469667694	21.92838616463871	4.688669556286664	8.014169582397688
11	35.71552909638449	28.293557539993387	19.13816213205215	16.852751231569968
12	37.48742319007084	15.366859888244816	24.86430971156022	22.281407210124115
13	24.540707086531935	15.299841593120616	31.232962556791477	28.926488763555973
14	31.1361776942225	16.319215972983795	28.646926732466447	23.89767960032726
15	26.451859975281565	14.021271780945915	24.83228018869567	34.69458805507685
16	31.192751579716955	20.629101606698345	27.15442059637579	21.023726217208903
17	38.88540741901231	14.901561439239646	21.32034745069368	24.892683691054362
18	29.193517503089804	15.478092850801609	30.410987518930494	24.917402127178097
19	37.14032064337564	17.051195014535136	20.82284540533013	24.9856389367591
20	27.955506814977284	17.03169878322628	25.432833742406043	29.57996065939039
21	32.33171444984072	16.666492593172837	29.066791999582225	21.935000957404217
22	31.61505387574634	15.563911083259352	24.997475934339477	27.823559106654827
23	33.871046355771405	16.69521471965464	28.2230577749926	21.210681149581355
24	39.157310216373354	16.35107142235452	21.71149059132766	22.78012776994447
25	30.220377043187636	16.12320921893223	28.401134959179764	25.25527877870037
26	35.46747436767803	16.42609709819486	22.77212038922833	25.334308144898777
27	31.061848312357476	15.276689818441348	26.17177572371055	27.489686145490627
28	37.69509286820896	18.839452016641424	18.712900586627672	24.75255452852194
29	37.48394172019427	19.57856807143976	19.24243215485578	23.69505805351019
30	35.65617003498877	19.821922815812837	20.554250004351836	23.967657144846555
31	36.556652218566676	20.812052848712725	18.83301129736975	23.798283635350845
32	36.738210872630425	20.50550942607969	20.237610319076715	22.518669382213172
33	34.149912092885614	20.804045467996588	21.25211064111268	23.79393179800512
34	30.21393632391596	22.75941302417881	24.54749595279127	22.479154699113966
35	29.96918899159225	26.27674204048949	21.935523177885706	21.81854579003255
36	30.197225268508365	26.199453409229378	20.778282590909882	22.825038731352375
37	31.75692377321705	25.805525092694136	19.189339739237905	23.248211394850905
38	30.981252284714607	26.206764495970198	19.568645882291502	23.243337337023693
39	31.839260535798214	23.904120319598935	20.84390829808345	23.4127108465194
40	30.385050568349957	23.30530750082685	19.176284227200725	27.13335770362247
41	26.976865632670112	24.799380298361967	20.55198704893206	27.67176702003586
42	26.76397374971713	21.694257315438577	22.644524518251604	28.897244416592688
43	27.33859035284697	20.760701168033144	24.489181332358523	27.41152714676136
44	28.069350879941513	21.40111755183038	23.44543666335927	27.084094904868834
45	29.378905774017788	21.276132783261094	22.274792417358608	27.070169025362507
46	27.912684735495326	23.798979929326162	22.16669277769074	26.121642557487775
47	27.562100718923528	22.653924486918378	22.41074381603913	27.37323097811896
48	28.682785872195243	23.649973018608456	23.384859087506747	24.282382021689557
49	30.521872334499626	22.508050899089596	21.40929900604035	25.560777760370428
50	26.547426323393736	22.90528661200759	22.99180113844065	27.555485926158024
51	27.26391282399429	24.465681410691595	22.188626037913206	26.08177972740091
52	27.588211742997892	23.89559071840131	22.139363239159575	26.376834299441228
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	2.0
7	1.5
8	1.0
9	12.5
10	24.0
11	48.0
12	72.0
13	169.0
14	462.5
15	659.0
16	567.0
17	475.0
18	454.0
19	433.0
20	603.5
21	774.0
22	736.5
23	699.0
24	708.5
25	718.0
26	1046.5
27	1375.0
28	1469.0
29	1563.0
30	2087.0
31	2611.0
32	3633.5
33	4656.0
34	5118.0
35	5580.0
36	6623.5
37	7667.0
38	7813.5
39	8575.0
40	9190.0
41	9037.5
42	8885.0
43	9783.5
44	10682.0
45	11280.0
46	11878.0
47	13013.0
48	14148.0
49	14457.0
50	14766.0
51	17997.5
52	21229.0
53	23286.5
54	25344.0
55	29870.0
56	34396.0
57	40832.0
58	47268.0
59	53970.0
60	60672.0
61	59092.5
62	57513.0
63	61246.0
64	56904.5
65	48830.0
66	43981.0
67	39132.0
68	31046.0
69	22960.0
70	19163.0
71	15366.0
72	13310.5
73	11255.0
74	12039.0
75	12823.0
76	8885.0
77	4947.0
78	3357.5
79	1768.0
80	1177.0
81	586.0
82	423.5
83	261.0
84	146.5
85	32.0
86	23.5
87	15.0
88	10.0
89	4.5
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	574470.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	12.75889080369732
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.61907880375465	8.882622243111042
2	9.443898712071599	2.409873448569986
3	3.5472604234883214	1.3577732518669383
4	1.717692643527614	0.8766341149233206
5	1.0600851342501638	0.6762755235260326
6	0.7981335952848723	0.6109979633401221
7	0.5880266317397947	0.5251797308823785
8	0.4911591355599214	0.5013316622277926
9	0.4338572364112639	0.49819833933886887
>10	8.84768609473914	29.5632496039828
>50	2.2620606854398604	19.798945114627394
>100	1.1405806592447065	27.111424443400008
>500	0.03274394237066143	2.9049384650199315
>1k	0.017736302117441607	4.282556095183387
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC	4721	0.8218009643671559	No Hit
CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA	3108	0.5410204188208262	No Hit
CTGTGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT	2624	0.45676884780754434	No Hit
CTGTGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC	2480	0.4317022646961547	No Hit
CTGTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG	2034	0.35406548644837854	No Hit
CTGTGTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC	1656	0.2882657057809807	No Hit
CTGTGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT	1252	0.2179400142740265	No Hit
CTGTGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG	1216	0.21167336849617907	No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC	1176	0.20471042874301532	No Hit
CTGTGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG	1151	0.20035859139728793	No Hit
CTGTGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG	1122	0.1953104600762442	No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT	1044	0.1817327275575748	No Hit
CTGTGTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC	1018	0.17720681671801836	No Hit
CTGTGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT	998	0.17372534684143645	No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC	888	0.15457726252023604	No Hit
CTGTGTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA	874	0.15214023360662873	No Hit
CTGTGTGGGAGACCGACCCTCGGCTCCGGAGCGGCAGTGCCGTCCCTCTCGG	861	0.14987727818685048	No Hit
CTGTGTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG	856	0.149006910717705	No Hit
CTGTGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC	838	0.14587358782878132	No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAA	768	0.1336884432607447	No Hit
CTGTGTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG	763	0.1328180757915992	No Hit
CTGTGTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA	728	0.12672550350758088	No Hit
CTGTGTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA	720	0.12533291555694814	No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAA	695	0.12098107821122078	No Hit
CTGTGTGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT	677	0.11784775532229708	No Hit
CTGTGTGGGGCTCAGCCCGTAGCCCGTCGGTTCCGGAGTAAGTTCCAGGTGG	656	0.11419221195188609	No Hit
CTGTGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT	645	0.11227740351976605	No Hit
CTGTGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT	635	0.1105366685814751	No Hit
CTGTGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC	635	0.1105366685814751	No Hit
CTGTGTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA	625	0.10879593364318414	No Hit
CTGTGTGGGACCTCTGAAGACATTTAAGAGGTTCTCCTGCCCCCGGGCGTGG	613	0.10670705171723502	No Hit
CTGTGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC	598	0.1040959493097986	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	5.22220481487284E-4	0.0	0.0	0.0	0.0
8	0.001044440962974568	0.0	0.0	0.0	0.0
9	0.0022629554197782305	0.0	0.0	0.0	0.0
10	0.019670304802687694	0.0	0.0	0.0	0.0
11	0.07572196981565617	0.0	0.0	0.0	0.0
12	0.09138858426027469	0.0	0.0	0.0	0.0
13	0.10496631677894407	0.0	0.0	0.0	0.0
14	0.11784775532229708	0.0	0.0	0.0	0.0
15	0.13177363482862464	0.0	0.0	0.0	0.0
16	0.15666614444618518	0.0	0.0	0.0	0.0
17	0.16658833359444358	0.0	0.0	0.0	0.0
18	0.1780771841871638	0.0	0.0	0.0	0.0
19	0.1881734468292513	0.0	0.0	0.0	0.0
20	0.19879192995282607	0.0	0.0	0.0	0.0
21	0.20836597211342628	0.0	0.0	0.0	0.0
22	0.2181140877678556	0.0	0.0	0.0	0.0
23	0.22925479137291765	0.0	0.0	0.0	0.0
24	0.23830661305203057	0.0	0.0	0.0	0.0
25	0.25014361063240903	0.0	0.0	0.0	0.0
26	0.2626769021881038	0.0	0.0	0.0	0.0
27	0.2778212961512351	0.0	0.0	0.0	0.0
28	0.2985360419168973	0.0	0.0	0.0	0.0
29	0.3161174647936359	0.0	0.0	0.0	0.0
30	0.33352481417654534	0.0	0.0	0.0	0.0
31	0.3575469563249604	0.0	0.0	0.0	0.0
32	0.3841802008808119	0.0	0.0	0.0	0.0
33	0.4115097394119797	0.0	0.0	0.0	0.0
34	0.44005779239995124	0.0	0.0	0.0	0.0
35	0.4717391682768465	0.0	0.0	0.0	0.0
36	0.5028983236722544	0.0	0.0	0.0	0.0
37	0.5399759778578516	0.0	0.0	0.0	0.0
38	0.5699166187964558	0.0	0.0	0.0	0.0
39	0.6024683621424965	0.0	0.0	0.0	0.0
40	0.628927533204519	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTAGT	35	1.0183794E-7	46.000004	21
GTACCGA	35	1.0183794E-7	46.000004	39
GTCGTAC	35	1.0183794E-7	46.000004	31
TACCGAC	35	1.0183794E-7	46.000004	40
TGCCGAT	35	1.0183794E-7	46.000004	18
TCACGGT	20	6.308805E-4	46.0	26
TAATATC	50	1.6370905E-11	46.0	39
CGGAAAT	20	6.308805E-4	46.0	39
GTATTGC	20	6.308805E-4	46.0	9
GCGAATT	25	3.415006E-5	46.0	26
CTATCCA	25	3.415006E-5	46.0	38
CGCATGA	40	5.6024874E-9	46.0	43
ATTACGG	20	6.308805E-4	46.0	26
GGTCCTA	20	6.308805E-4	46.0	9
CCTACTT	20	6.308805E-4	46.0	16
CACGCCA	40	5.6024874E-9	46.0	29
TATATTT	20	6.308805E-4	46.0	43
ATTAATC	40	5.6024874E-9	46.0	35
ATTGTAC	20	6.308805E-4	46.0	23
GTCTATT	40	5.6024874E-9	46.0	16
>>END_MODULE