FastQCFastQC Report
Fri 17 Jun 2016
SRR1527511_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1527511_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739689
Sequences flagged as poor quality0
Sequence length52
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTC59160.7997955897681323No Hit
CTGTGTGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCT36580.49453216148949086No Hit
CTGTGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAA35090.47438856059776474No Hit
CTGTGTGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTC32940.44532229085467No Hit
CTGTGTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCG27210.36785730219051516No Hit
CTGTGTGGGGATGCCGACTGGCGATGCGGCGGCGTTATTCCCATGACCCGCC23360.3158084005575316No Hit
CTGTGTGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCG15610.21103463753009713No Hit
CTGTGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCT15550.21022348581633635No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTC14570.19697467449157685No Hit
CTGTGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGG14560.1968394825392834No Hit
CTGTGTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCT13580.18359067121452394No Hit
CTGTGTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCT13040.1762903057906769No Hit
CTGTGTGGGGACGGCAGTGGTGCTGGGAGTCTCGTGTCCGTGGTGCCGTTAC12570.16993628403288408No Hit
CTGTGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAG12390.16750282889160176No Hit
CTGTGTGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGC11710.1583097761356462No Hit
CTGTGTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTC11400.15411882561454882No Hit
CTGTGTGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGA11180.1511446026640926No Hit
CTGTGTGGGAGACCGACCCTCGGCTCCGGAGCGGCAGTGCCGTCCCTCTCGG10750.14533134871547365No Hit
CTGTGTGGGGAGTCCGAGTGGAGCGAGCGAGTCGAGCGGTTGTCTGGTCGCG10410.1407348223374959No Hit
CTGTGTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAA10400.14059963038520243No Hit
CTGTGTGGGGAGATGCGCCACGGCTTCGGTAGCGACCGGCTGTCTTCTGCTG9560.12924350639255147No Hit
CTGTGTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGA9230.12478217196686715No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGGAAAAAAAAAAAAAAAAAAAAA9230.12478217196686715No Hit
CTGTGTGGGGCTCAGCCCGTAGCCCGTCGGTTCCGGAGTAAGTTCCAGGTGG8900.12032083754118285No Hit
CTGTGTGGGGGAGTGGCCTTGTTCTCGATACTTCGTTGTGGTTGTGAACTCT8780.11869853411366128No Hit
CTGTGTGGGGACAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTT8480.11464277554485737No Hit
CTGTGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGC8310.11234451235586848No Hit
CTGTGTGGGACCTCTGAAGACATTTAAGAGGTTCTCCTGCCCCCGGGCGTGG8180.11058701697605346No Hit
CTGTGTGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCC7950.10747760207330379No Hit
CTGTGTGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCAT7930.10720721816871684No Hit
CTGTGTGGGGAGTGCAGTGCTCTGTGTGGGAAAAAAAAAAAAAAAAAAAAAA7920.1070720262164234No Hit
CTGTGTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCA7730.10450337912284756No Hit
CTGTGTGGGGAGACCGACCCTCGGCTCCGGAGCGGCAGTGCCGTCCCTCTCG7580.10247549983844562No Hit
CTGTGTGGGATTGCCTTTTGGCACTCGGGAGACAAGATGCAGGTTCCGTCTC7510.10152915617239136No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTATA206.309987E-446.00000413
TAATATC351.0189069E-746.00000439
AACAACT206.309987E-446.00000413
AGCCGAT351.0189069E-746.00000429
AGTTTAT206.309987E-446.00000415
CGTATAC206.309987E-446.00000420
GGTCGAC405.6061253E-946.0000049
TTAGCTA206.309987E-446.00000434
TTAGATG405.6061253E-946.00000420
GACCGTT206.309987E-446.00000443
TAGCGAT206.309987E-446.00000424
TAAGTTG206.309987E-446.00000417
ATGCTAG206.309987E-446.00000411
GTCTAAA206.309987E-446.00000411
TAAGTCG206.309987E-446.00000443
CCTAAAT405.6061253E-946.00000440
ATCACAA206.309987E-446.00000435
ATACTTA206.309987E-446.00000414
CCGTCGA206.309987E-446.00000444
AGTATAC351.0189069E-746.00000440
Produced by FastQC (version 0.11.5)